Anti-Inflammatory and Analgesic Effects and Potential Targets of Shenzhu Jiedu Granule Against Novel Coronavirus Pneumonia Based on Network Pharmacology.

Objective: The objective of this study was to investigate the preventive and therapeutic effects of Shenzhu Jiedu Granule on COVID-19 using network pharmacology and animal experiments. Methods: Obtain the chemical components of Shenshu Jiedu Granule from the online pharmacology database and analysis platform (ETCM) of the Chinese traditional medicine system, obtain the potential target of the compound through the UniProt database, and obtain the related target of COVID-19 from GeneCards and OMIM databases; Construct a component target network diagram using Cytascape 3.7.0 software, import the protein interaction (PPI) of intersection targets into Cytascape software through STRING database, and use the Metascape platform to conduct gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomics (KEGG) enrichment analysis on intersection targets.To explore its anti-inflammatory and analgesic effects through animal ear swelling, hot plate and torsion experiments. Results: Analysis revealed 72 key target proteins associated with the effects of Shenzhu Jiedu Granule demonstrated that mainly interleukin-6 (IL-6), interleukin-1 ß (IL 1 ß), B cells κ Light peptide gene enhancer nuclear factor inhibitor 1 (NFKB1), B cells κ Light peptide gene enhancer nuclear factor inhibitor 1B (NFKB1A), interferon ß IFNB1, tumor necrosis factor TNF, recombinant human mitogen activated protein kinase 12 (MAPK12), serine/threonine kinase 1 (AKT1), B cells κ Light peptide gene enhancer inhibitor kinase ß (IKBKB), etc.The analysis found that it is mainly related to multiple biological processes such as intercellular immune regulation, inflammatory cytokines, and ion channels in the microenvironment; KEGG analysis showed that COVID-19 pathway, influenza virus pathway and multiple immune inflammatory response pathways were mainly involved. Obtained 91 effective ingredients of Shenshu Jiedu Granule, 10 anti-inflammatory, bactericidal, and antiviral compounds, and 4 immune enhancing compounds. Shenzhu Jiedu Granule demonstrated an inhibitory effect on xylene-induced ear swelling in mice and significantly enhanced the anti-inflammatory and analgesic effects by reducing body twists and prolonging the time mice licked their feet. Conclusions: It is suggested that Shenzhu Jiedu Granule has anti COVID-19, influenza virus, antibacterial and anti-inflammatory effects, and can significantly enhance the anti-inflammatory and analgesic effects of mice, which highlight the significance of the study in the context of current global health concerns.

[Study of Jinye Baidu granule in treatment of wind-warmth lung heat disease (heat in lung-wei pattern)： a randomized, double-blind, parallel-controlled trial].

A randomized, double-blind, active parallel controlled trial will be conducted to include patients with acute upper respiratory infection and wind-warmth lung heat disease (heat in lung-wei pattern). Patients with serious bacterial infection (white blood cell count>1.2×1010, neutrophil>80%) will be excluded.so as to further identify Jinye Baidu Granule indications of Wind-warmth lung heat disease (heat in lung-wei pattern).According to the subjects of symptoms of fever and sore throats,Patients will be divided into three blocks： fever, swelling and sore throat, combined fever and swelling and sore throat. Subjects in three blocks will be divided into treatment group and control group with stratified blocked randomization. The treatment group will be treated with Jinye Baidu Granule, and the control group will be treated with Fufang Shuanghua granule. Primary outcome measure of patients with fever will be body temperature recovery time. Primary outcome measure of patients with swelling and sore throat will be throat symptom score. Primary outcome measures of patients combined with fever and swelling and sore throat will be body temperature recovery time and throat symptom score. Before and after all of the patients in the group were collected blood and urine routine, liver and renal function, electrocardiogram (ECG) data as the safety index while observing it adverse events. A total of 623 patients were included. Compared with control group,sore throat symptoms of patients with sore throat and pharyngeal symptoms total score have significantly different. so Jinny Baidu granule have a significant advantage in the treatment of sore throat. All of the experiments showed that the safety of the drug was good. Nausea, vomiting, stomach pain, diarrhea, digestive tract symptoms may occur only occasionally. We should pay attention to the changes when using in patients with spleen deficiency cold to avoid adverse reaction.

[Treatment of upper respiratory infection by fangfeng tongsheng granule: a randomized controlled study].

OBJECTIVE: To evaluate the clinical effectiveness and safety of Fangfeng Tongsheng Granule (FTG) in the treatment of upper respiratory infection (superficial cold and interior heat syndrome, exterior and interior excess syndrome). METHODS: A randomized, double-blinded, multi-centered, placebo-parallel-controlled clinical trial was adopted. Totally 324 patients were enrolled and assigned to two groups, 216 patients in the treatment group and 108 patients in the control group. Those in the treatment group took FTG at the daily dose of 3 g, twice per day, the therapeutic course being 3 days. Those with axillary temperature more than 37 degrees C took one more time before medication. Those in the control group took simulated agent granules the same dose and dosage as the treatment group. The effect of Chinese medical syndrome (ECMS), the rate of temperature-dropping-to-normal (RT), the time of temperature-dropping-to-normal (TT), the curative effect of single symptom (CESS) and adverse reactions were observed. RESULTS: Totally 203 completed the trial in the treatment group and 101 in the control group. In the treatment group, the cured-effective rate was 55.67% (113/ 101), the total effective rate was 93.10% (189/101), the ECMS score decreased by 9.24 +/- 4.46, while they were 5.94% (6/101), 36.63% (37/101), and 3.27 +/- 3.29, respectively in the control group (P < 0.01). The RT was 87.50% (98/112) in the treatment group and 58.49% (31/53) in the control group (P < 0.01). The TT in the treatment group was superior to that of the control group (P < 0.01). As for CESS, all of the three primary symptoms and nine secondary symptoms were improved more obviously in the treatment group than in the control group. The integral decreased obviously, showing statistical difference (P < 0.01). The decrease was more obvious in the treatment group than in the control group (P < 0.01). There was no adverse event related to FTG. CONCLUSION: FTG was effective and safe in treating upper respiratory infection (superficial cold and interior heat syndrome, exterior and interior excess syndrome).

[Evaluation on effect of integrative medical treatment on quality of life of rehabilitation stage in 85 patients with SARS].

OBJECTIVE: To evaluate the effect of integrative medical treatment (IMT) with serial Chinese recipes on quality of life (QOF) of rehabilitation stage in SARS patients. METHODS: Eighty-five SARS patients of rehabilitation stage were enrolled in the clinical study. They were divided into the IMT group (62 patients received serial Chinese recipes and western medicine) and the control group (23 patients received western medicine alone). The serial Chinese recipes were given according to patients' syndrome, one dose per day for oral intake for 3 weeks. QOF scoring in patients was observed. RESULTS: QOF scoring in the IMT group before treatment was not significantly different from that in the control group. After 3 weeks treatment, it improved to some extent in both groups, but the improvement in the IMT group was superior to that in the control group in respect of total score and score of psychologic emotional factors. CONCLUSION: Serial Chinese recipe could improve QOF of rehabilitation stage in SARS patients.