Low-cost and sensitive chemiluminescence detection of phthalates in environment by signal sensing of carbon-based materials from PVC/coal gangue dechlorination.

BACKGROUND: The detection of plasticizers in the environment is important to prevent environmental risks and people's health hazards. Improving recycling efficiency of waste PVC still faced challenges. RESULTS: In this work, it was found that solid products from waste PVC/coal gangue dechlorination in subcritical water (dPVC) had strong catalysis activity for luminol-H2O2 chemiluminescence (CL) reaction. Phthalates, common plasticizers, could bond and adsorb on dPVC, which greatly inhibited the luminol-H2O2-dPVC CL reaction. Based on this, a low-cost CL analysis was constructed for the detection of phthalates combinations (PACs) and di-(2-ethylhexyl) phthalate (DEHP) in the environment. The detection limit for PACs and DEHP was 0.048 ng/L and 0.13 ng/L, respectively. Compared with HPLC standard method, the dPVC CL analysis had accuracy and reliability for the detection of phthalates in actual environmental samples. Besides, the results of life cycle assessment (LCA) revealed that dPVC for CL sensing materials had significantly small global warming potential (GWP). SIGNIFICANCE: The use of dPVC for CL sensing not only improved the recycling efficiency of PVC, but also reduced carbon emissions of obtaining CL sensing materials.

Upcycling of waste disposable medical masks to high value-added gasoline fuel and surfactants products by sub/supercritical water degradation and partial oxidation.

The amount of waste disposable medical masks (DMMs) and the potential environmental risk increased significantly due to the huge demand of disposable medical surgical masks. In this study, two effective and environmentally friendly processes, supercritical water degradation (SCWD) and subcritical water partial oxidation (SubCWPO), were proposed for the upcycling of DMMs. The optimal conditions for the SCWD process (conversion ratio>98 %) were 410 â„ƒ, 15 min, and 1:5 g/mL. The oil products obtained from the SCWD process were mainly small molecule hydrocarbons (C7-C12) with a content of 86 % and could be recycled as fuel feedstock for gasoline. Alkyl radicals in the SCWD reaction formed double bonds and ring structures through hydrogen capture reactions, ß-scission, and dehydrogenation reactions, and aromatic hydrocarbons were formed by olefin cyclization and cycloalkane dehydrogenation. The introduction of an oxidant (H2O2) to the reaction system could significantly reduce the reaction temperature and shorten the reaction time. At 350 â„ƒ, 15 min, 1:20 g/mL, V(H2O2): V (H2O) of 1:1, the conversion ratio of the SubCWPO process was 88 %, which was higher than that of the SCWD process at 400 â„ƒ (71.49 %). Oil products produced from the SubCWPO process were rich in alcohols and esters, which could be used as raw materials for nonionic surfactant of polyol and fatty acid ester. The abundant hydroxyl radical in the SubCWPO system trapped hydrogen atoms on PP and reacted with the resulting alkyl radical to form alkanols, which was oxidized to form acids. The esterification of acids and alkanols formed high level of esters. The SCWD and SubCWPO processes proposed in this study are believed to be promising strategies for DMMs degradation and the recovery of high value-added hydrocarbons.

CRISPR/Cas9-based genome-wide screening for metastasis ability identifies FCGR1A regulating the metastatic process of ovarian cancer by targeting LSP1.

BACKGROUND: Metastasis is a main cause of death from ovarian cancer (OC). Identifying key markers involved in OC metastasis can aid in the effective detection of early postoperative metastasis. However, the role of FCGR1A in OC metastasis has yet to be fully established. A genome-wide CRISPR/Cas9-based screening system was used to identify regulatory factors involved in metastasis. METHODS: The expression of FCGR1A and LSP1 in ovarian cancer cell lines was examined by quantitative real-time polymerase chain reaction (qRT‒PCR). The functions of FCGR1A and LSP1 in OC cell migration, invasion and proliferation were determined using wound healing, Transwell invasion and CKK-8 assays. A transcription-activated library was used to identify the potential downstream genes of FCGR1A. FCGR1A expression was detected by immunohistochemistry and the immunity risk score (IRS) scores were calculated. RESULTS: FCGR1A was upregulated in OC cells compared with normal ovarian cells. Downregulation of FCGR1A inhibited metastasis, proliferation and epithelial-mesenchymal transition (EMT) progression in OC cells in vitro and intraperitoneal metastasis in vivo. Moreover, downregulation of FCGR1A was accompanied by decreased LSP1 expression. Overexpression of LSP1 partially reversed the tumor suppressive effect of FCGR1A downregulation. Higher FCGR1A expression was related to metastasis, higher grade, higher stage, and lymph node metastasis in OC. Survival analysis suggested that the group with higher FCGR1A expression had a lower tumor-free survival rate and a lower overall survival rate than did the group with low FCGR1A expression. CONCLUSIONS: FCGR1A enhances OC metastasis by regulating LSP1, and FCGR1A is associated with poor prognosis, suggesting that FCGR1A is a potential predictive factor for detecting early postoperative metastasis.

A modified cultivation strategy to enhance biomass production and lipid accumulation of Tetradesmus obliquus FACHB-14 with copper stress and light quality induction.

In this study, a two-stage culture strategy was refined to concurrently enhance the growth and lipid accumulation of Tetradesmus obliquus. The results unveiled that, during the initial stage, the optimal conditions for biomass accumulation were achieved with 0.02 mg·L-1 Cu2+ concentration and red light. Under these conditions, biomass accumulation reached 0.628 g·L-1, marking a substantial 23.62 % increase compared to the control group. In the second stage, the optimal conditions for lipid accumulation were identified as 0.5 mg·L-1 Cu2+ concentration and red light, achieving 64.25 mg·g-1·d-1 and marking a 128.38 % increase over the control. Furthermore, the fatty acid analysis results revealed an 18.85 % increase in the saturated fatty acid content, indicating enhanced combustion performance of microalgae cultivated under the dual stress of red light and 0.5 mg·L-1 Cu2+. This study offers insights into the potential application of Tetradesmus obliquus in biofuel production.

A novel subcritical water synergistic co-treatment of brominated epoxy resin and copper-based spent catalysts: debromination, phenol production, and copper recovery.

In this study, a high-efficiency co-treatment strategy for brominated epoxy resin (BER) and copper-based spent catalyst (CBSC) was developed by using subcritical water (SubCW) process. Multivalent species of copper released from CBSC could accelerate the electron transfer of the SubCW system and efficiently catalyze radical reactions to promote the debromination and decomposition of BER, and had an effect on the capture and binding of bromine species. Meanwhile, the formation of HBr by the BER debromination resulted in a decrease in the system pH and markedly enhanced the leaching/recovery of Cu from CBSC. The optimal conditions of the SubCW co-treatment process were as follows: reaction temperature of 350 °C, solid-to-liquid ratio of 1:30 g/mL, BER-to-CBSC mass ratio of 10:1 g/g, and reaction time of 60 min. Under the optimal conditions, 97.12 % of the Br could be removed from BER by the SubCW co-treatment process and a high-purity phenol (64.09 %) could be obtained in the oil phase product, and 86.44 % of Cu in the CBSC could be leached and recovered. The introduction of CBSC significantly changed the decomposition path of BER. Compared to the SubCW process without CBSC, bromine-free oils products could be obtained by the co-treatment process of BER and CBSC at low-temperature. This study provided a novel understanding of resource conversion mechanism of BER and CBSC in subcritical water medium via the synergistic effect between the two different waste streams to improve treatment efficiency and synchronously recover high-value products.

Exploring the drivers of agricultural wages growth in China: A comprehensive framework utilizing input-output and structural decomposition methods.

This study explores the factors driving agricultural wages growth in China from 1981 to 2020. We propose a comprehensive framework that combines input-output analysis and structural decomposition analysis to investigate the drivers of agricultural wages growth from four perspectives: supply, demand, industrial linkages, and agricultural support policies. The findings indicate that changes in consumer demand, investment demand, and labor mobility play significant roles in driving the growth of agricultural wages in China. Additionally, agricultural support policies have contributed to an increase in agricultural wages to some extent. However, changes in industrial linkages negatively affect agricultural wages growth. A notable strength of this study lies in the methodology employed, which ensures a comprehensive and systematic analysis encompassing diverse factors rather than a restricted perspective.

Different effects of CO2 laser and estrogen treatment on vaginal mucosa microbiota and function in genitourinary syndrome of menopause patients.

AIM: To characterize the effects of CO2 laser treatment and estrogen treatment on vaginal microbiota in patients with genitourinary syndrome of menopause (GSM). METHODS: Sixty-four patients with genitourinary syndrome were divided into the estrogen group, the CO2 laser group, and the control group. The control group did not receive any treatment. Vaginal mucosa was collected after 3 and 12 months of treatment. The former was used for 16S rRNA sequencing, and the latter was used for pathological evaluation. Vaginal health and voiding function were assessed using the vaginal health index (VHI) scale and the UDI-6 scale at 3 and 12 months after treatment. RESULTS: The results showed that both treatments reduced alpha diversity in the vaginal flora. Additionally, the abundance of 65 genera differed significantly between the treatment and control groups, with an increase in potentially beneficial bacteria such as Lactobacillus, IheB3_7, Mycoplasma urealyticum, and Streptococcus. In addition, the VHI and UDI-6 scores improved in both treatment groups compared to the control group after 3 months. Whereas VHI and UDI-6 scores were close to baseline in the estrogen group, and remained significantly improved in the CO2 laser group after 12 months. Pathological results showed that both methods improved the vaginal health status of patients with GSM after 12 months of treatment. However, the CO2 group exhibited a more significant increase in type III collagen. CONCLUSIONS: Both CO2 laser and estrogen therapies can regulate the vaginal flora imbalance of GSM and improve the corresponding symptoms. However, the long-term efficacy of CO2 laser therapy is superior compared to estrogen therapy.

Chemiluminescence sensing for Hg2+ in environment water using carbon materials from PVC dechlorination as signal initiator.

Chemiluminescence (CL) sensing with good performance remains a challenge. The utilization of secondary residues from polyvinyl chloride (PVC) treatment is the key to improve PVC recycling rate. Herein, dechlorinated carbon materials from PVC/iron scrap co-treatment in subcritical water were used as CL sensing element. It was found that tiny changes in the spatial structure of aptamer could cause huge changes in CL signal of the residue-luminol system. A CL biosensor was constructed for mercury in environment water for the first time. The detection limit was estimated to be 0.37 pM. High sensitivity was mainly due to strong CL triggering and signal amplification from residues and effective regulating residue activity by aptamer space dimension. For real water samples, the results by residue CL analysis were consistent with that by cold vapor atom adsorption spectroscopy (CVAAS). Most strikingly, the used material was secondary residues from the treatment of PVC waste, which reduced the time and energy consumption of CL sensing. This research proposed the approach for routine monitoring mercury in environment but also provided the reference for developing other environmentally beneficial analysis platforms.

Coated cysteamine, a potential feed additive for ruminants - An updated review.

For sustainable development, better performance, and less gas pollution during rumen fermentation, there is a need to find a green and safe feed additive for ruminants. Cysteamine (CS) is a biological compound naturally produced in mammalian cells. It is widely used as a growth promoter in ruminants because of its ability to control hormone secretions. It mainly controls the circulating concentration of somatostatin and enhances growth hormone production, leading to improved growth performance. CS modulates the rumen fermentation process in a way beneficial for the animals and environment, leading to less methane production and nutrients loss. Another beneficial effect of using CS is that it improves the availability of nutrients to the animals and enhances their absorption. CS also works as an antioxidant and protects the cells from oxidative damage. In addition, CS has no adverse effects on bacterial and fungal alpha diversity in ruminants. Dietary supplementation of CS enhances the population of beneficial microorganisms. Still, no data is available on the use of CS on reproductive performance in ruminants, so there is a need to evaluate the effects of using CS in breeding animals for an extended period. In this review, the action mode of CS was updated according to recently published data to highlight the beneficial effects of using CS in ruminants.

Housing wealth appreciation and heterogeneous household consumption: Evidence from China.

In this paper, we develop a DSGE model including heterogeneous households, introduce the financial friction of credit constraint mechanism, and study the impact of house price shocks on the consumption of heterogeneous household. Based on this, the CHFS data in 2011, 2013, 2015, 2017, and 2019 were used to test the marginal propensity to consume for housing wealth appreciation under different credit constraints. Results show that: Firstly, the financial accelerator mechanism plays an important role in the transmission of housing price shocks to household consumption. The looser the degree of credit constraints, the more obvious the rise in housing prices will be to the consumption expenditure of borrowing household. Secondly, the impact of housing wealth appreciation on household consumption under different credit constraints is heterogeneous. Among them, housing wealth appreciation has a significant positive impact on household consumption expenditure with multiple houses, credit cards, non-loan restrictions, while the marginal effect on the consumption expenditure of households with only one house, loan limited, and no credit cards decreases. Thirdly, for every 1% increase in the housing wealth appreciation, household consumption will increase significantly by 0.10-0.14%.

An alkali-enhanced subcritical water treatment strategy of short-chain chlorinated paraffins: Dechlorination and hydrocarbons recovery.

As persistent organic pollutants, short-chain chlorinated paraffins (SCCPs) have attracted wide attention in the field of environmental health risk and hazardous waste management. Efficient dechlorination of high content of SCCPs in plastic waste is the committed step for its detoxification and safety treatment. In this study, a high-efficiency and low-temperature process for dechlorination and hydrocarbons recovery from typical SCCPs (52#SCCPs) by subcritical water (SubCW) with alkali enhancer was developed. The introduction of alkali enhancer in the SubCW process had significantly enhanced effect on the dechlorination of 52#SCCPs, and the order of the enhanced effect of alkali enhancer for the dechlorination was NaOH > Na2CO3 > NaHCO3 > NH3·H2O > KOH. The dechlorination behaviors of 52#SCCPs in the NaOH-enhanced SubCW process were studied systematically under different conditions including temperature, residence time, alkali concentration, and volume ratio. The results showed that high-efficiency dechlorination (100 %) of 52#SCCPs could be achieved by the NaOH-enhanced SubCW process at low temperature for a short time (250 °C, 5 min). All of the chlorine released from the molecular chain of 52#SCCPs was transferred to the aqueous phase in the form of inorganic chlorine. The continuous HCl elimination reaction was the primary dechlorination mechanism for 52#SCCPs in the NaOH-enhanced SubCW process. After the dechlorination of 52#SCCPs, high value-added hydrocarbons such as 2,4-hexadiyne (31.74 %) could be obtained. The alkali-enhanced SubCW process proposed in this study is believed to be an environmentally friendly and high-efficiency method for dechlorination/detoxification and resource recovery of SCCPs.

Value of Thoracic Echocardiography in the Diagnosis of Acute Heart Failure: Systematic Review and Meta-analysis.

Context: For patients with acute heart failure (AHF), the methods of clinical diagnosis of pulmonary edema mainly include clinical symptoms, laboratory results, and an imaging examination. The common diagnostic methods, such as chest X-rays and computerized tomography (CT) scanning, haven't been completely satisfactory. Objective: The study intended to systematically, quantitatively, and comprehensively evaluate the value of a lung (pulmonary) ultrasound, performed at a patient's bedside, in the diagnosis of acute heart failure (AHF), to provide an objective basis for its clinical application and further research. Design: The research team searched PubMed, Excerpta Medica Database (EMBASE), ScienceDirect, Cochrane Library, China Journal Full-text Database (CNKI), VIP Full-text Database, Wanfang Database, and China Biomedical Literature Database (CBM) for relevant literature, from January 2010 to the present, about the use of a lung ultrasound for diagnosis of AHF patients. The team used keywords to search literature: ultrasound, AHF diagnosis, cardiogenic pulmonary edema, ultrasonic examination, AHF diagnosis, and cardiogenic pulmonary edema. The research team then conducted a meta-analysis of the collected data according to the Cochrane Handbook 5.3 with RevMan 5.3 statistical software. Setting: The study took place at Jinan. Outcome Measures: The research team: (1) evaluated the quality of the included studies; (2) examined the accuracy of a lung ultrasound in the diagnosis of AHF compared to computerized tomography (CT) as well as to the conventional ultrasonic cardiogram (echocardiogram) that a cardiologist performs; (3) determined the sensitivity, specificity, and predictive value of lung ultrasound using data from two of the included studies; (4) evaluated the data by drawing funnel charts; and (5) examined the publication bias of the included studies. Results: The research team selected six controlled clinical studies, with 345 data samples, for the meta-analysis. The team performed heterogeneity tests for the included research data. For the first test, the team compared the accuracy of lung ultrasound and CT in diagnosing AHF and found obvious heterogeneity, with χ2 = 11.40, df = 3, P = .010, and I2 = 74%. Based on an analysis using a random effects model, the team found no significant differences between the two methods in the diagnosis of AHF (P = .35). For the second test, the team compared the accuracy of lung ultrasound and an ultrasonic cardiogram in diagnosing AHF and found that the data didn't differ significantly, with χ2 = 0.08, df = 1, P = .78, I2 = 0%. Based on an analysis using a fixed effects model, the team found that the accuracy of the lung ultrasound in diagnosing AHF was significantly higher than that of ultrasonic cardiogram (P = .01). In the two studies, the sensitivity and specificity were high. The majority of the funnel charts were symmetrical, but a few were asymmetrical, suggesting a publication bias, which the heterogeneity in the studies and the limited number of examined examples may explain. Conclusions: Lung ultrasound is of great value in the diagnosis of AHF. It's highly efficient, has prospects for broad clinical application, and is worth popularizing, benefiting patients. Scholars need to verify the current study's findings in follow-up studies and in more high-quality case-control trials.

Ultrafast colorimetric detection of Cr(VI) based on competition of 8-HQ to Cr(VI) and TMB oxides using GO/AuNPs nanocomposites as peroxidase mimic.

Rapid detection of ultra-trace heavy metal chromium is very important for ecological environment. Herein, a rapid colorimetric assay was constructed for detecting hexavalent chromium (Cr(VI)) in environment water through the strong peroxidase mimicking activity of graphene oxide/gold nanoparticles (GO/AuNPs) nanocomposites and competition of Cr(VI) to 3,3',5,5'-tetramethylbenzidine (TMB) oxides and 8-hydroxyquinoline (8-HQ). Cr(VI) could effectively prevent the reaction between 8-HQ and TMB oxides to restore the blue color of the system. The detection limit for Cr(VI) was as low as0.018 µM by spectroscopic absorption. Paper-based colorimetric analysis had the detection limit of0.153 µM. The high sensitivity was basically due to the strong peroxidase mimicking activity of GO/AuNPs nanocomposite from synergistic coupling action and the firm chelation between 8-HQ and Cr(VI) from inner-sphere surface complexation. The detection results for real water sample showed that the analysis had feasibility in practical application. It is worth mentioning that the assay is performed by one-step mixing mode at room temperature, and a single test can be completed in half a minute. Indeed, this work not only provided an extremely easy method for real-time detecting Cr(VI) in the environment, but also verified the vitality of colorimetric strategy based on the strong peroxidase mimicking activity and competitive reaction.

Treatment of DEHP-rich PVC waste in subcritical urine wastewater: Efficient dechlorination, denitrification, plasticizer decomposition, and preparation of high-purity phthalic acid crystals.

It is difficult to dispose diethylhexyl phthalate-rich polyvinyl chloride (DEHP-rich PVC) waste due to the high level of chlorine and plasticizer. On the other hand, the denitrification of urine wastewater with high nitrogen content also faces great challenges. In this study, a synergistic treatment strategy was developed for the DEHP-rich PVC waste and urine wastewater by a subcritical water process. Subcritical urine wastewater (SUW) was used as a reaction medium in the synergistic treatment. PVC dechlorination, DEHP decomposition, and denitrification of urine wastewater were synchronously achieved in the one pot SUW. Under the optimal conditions (300 °C, 15 min, 1:5 g/mL), the PVC dechlorination ratio, urine wastewater denitrification ratio and DEHP decomposition ratio could reach 98.4%, 64.9%, and 99.2%, respectively. The decomposition of DEHP mainly included hydrolysis, nucleophilic substitution, and acylation. DEHP could be converted into phthalic acid crystal at 220 °C with a yield of 66.25% due to the efficient hydrolysis action of SUW. All the removed Cl was transferred from PVC matrix to aqueous phase. Hydroxyl nucleophilic substitution is the principal dechlorination path of PVC. The reactions between N-containing species and DEHP in SUW resulted in the high-efficiency denitrification of urine wastewater, and the N element was fixed in solid residue or transferred to oil phase as amides compounds. It is believed that the proposed SUW process is a promising technology for the synergistic treatment of DEHP-rich PVC waste and urine wastewater.

Terminal repeats impact collagen triple-helix stability through hydrogen bonding.

Nearly 30% of human proteins have tandem repeating sequences. Structural understanding of the terminal repeats is well-established for many repeat proteins with the common α-helix and ß-sheet foldings. By contrast, the sequence-structure interplay of the terminal repeats of the collagen triple-helix remains to be fully explored. As the most abundant human repeat protein and the most prevalent structural component of the extracellular matrix, collagen features a hallmark triple-helix formed by three supercoiled polypeptide chains of long repeating sequences of the Gly-X-Y triplets. Here, with CD characterization of 28 collagen-mimetic peptides (CMPs) featuring various terminal motifs, as well as DSC measurements, crystal structure analysis, and computational simulations, we show that CMPs only differing in terminal repeat may have distinct end structures and stabilities. We reveal that the cross-chain hydrogen bonding mediated by the terminal repeat is key to maintaining the triple-helix's end structure, and that disruption of it with a single amide to carboxylate substitution can lead to destabilization as drastic as 19 °C. We further demonstrate that the terminal repeat also impacts how strong the CMP strands form hybrid triple-helices with unfolded natural collagen chains in tissue. Our findings provide a spatial profile of hydrogen bonding within the CMP triple-helix, marking a critical guideline for future crystallographic or NMR studies of collagen, and algorithms for predicting triple-helix stability, as well as peptide-based collagen assemblies and materials. This study will also inspire new understanding of the sequence-structure relationship of many other complex structural proteins with repeating sequences.

A novel conversion strategy for organic compounds in waste liquid crystal displays based on the near/supercritical methanol process.

Waste liquid crystal displays (LCD) contain a large number of organic compounds such as cellulose triacetate (CTA), poly(vinyl alcohol) (PVA), triphenyl phosphate (TPP), and liquid crystal (LC). It is important to recover organic compounds from waste LCD due to their value and environmental toxicity. However, it is challenging to recover organic compounds from waste LCD because of the heterogeneous mixture of glass, organics and metals contained therein. In this study, an environment-friendly near/supercritical methanol (NSCM) process was developed as a closed cycle technology for the conversion of organic compounds from waste LCD. The acid-base catalytic activity and nonpolar property of the NSCM could efficiently promote the conversion of organic compounds from waste LCD. TPP could be extracted below 200 °C in the NSCM process. Below 250 °C, the conversion ratio of organic compounds from waste LCD ranged from 5 % to 68 % due to the extraction or decomposition of TPP, LC, and PVA. The main products obtained at 250 °C included long-chain alcohols and alkanes with a similar composition to industrial liquid paraffin, which could be widely used in other industrial processes. Under the optimal operation parameters (300 °C, 30 min, and 1:20 g/ml), the conversion ratio of organic compounds could reach 98 % due to the efficient decomposition of CTA. The main products obtained included ketones and esters chemicals, which could be further used as a chemical feedstock. No secondary pollutant was generated in the whole process. The low-boiling methanol could easily be recycled, which could make the NSCM a clean process for the production of high value-added organic products from waste LCD.

Effect of supraglottic airway devices versus endotracheal intubation general anesthesia on outcomes in patients undergoing mechanical thrombectomy: A prospective randomized clinical trial.

BACKGROUND: There are still controversies about the optimal anesthesia protocol for patients with acute ischemic stroke (AIS) undergoing mechanical thrombectomy (MT). The aim of this study was to explore the effect of supraglottic airway device (SAD) versus endotracheal intubation (EI) general anesthesia on clinical and angiographic outcomes in patients with AIS undergoing MT. METHODS: One hundred sixteen patients with large-vessel occlusion stroke were randomized to receive either SAD or EI general anesthesia. The primary outcome was the rate of occurrence of >20% fall in mean arterial pressure (MAP). Secondary outcomes included hemodynamics, successful recanalization, time metrics, satisfaction score of neurointerventionalist, number of passes performed, the conversion rate from SAD to EI, the National Institutes of Health Stroke Scale score, and Alberta Stroke Program Early CT Score before and 24 hours after surgery, length of stay in the stroke unit and hospital, complications and functional independence at discharge, and 90 days after stroke. RESULTS: Both the lowest systolic blood pressure and lowest diastolic blood pressure were significantly lower in the EI group (P = .001). The consumption of vasoactive agents, the occurrence of >20% reduction in MAP and time spent with >20% fall in MAP were significantly higher in the EI group (P < .05). Compared with the EI group, the time for door-to-puncture was significantly shorter in the SAD group (P = .015). There were no significant differences with respect to puncture-to-reperfusion time, number of passes performed, rates of successful recanalization, National Institutes of Health Stroke Scale score, and Alberta Stroke Program Early CT Score 24 hours after surgery. The satisfaction score of neurointerventionalist was significantly lower in the EI group (P = .043). Conversion rate from SAD to EI was 7.41%. There were no significant differences with respect to complications, mortality, and mean Modified Rankin Scale scores both at discharge and 90-day after stroke. However, length of stroke unit and hospital stays were significantly shorter in the SAD group (P < .05). CONCLUSION: AIS patients undergoing MT with SAD general anesthesia led to more stable hemodynamics, higher satisfaction score of neurointerventionalist, shorter door-to-puncture time, length of stroke unit, and hospital stay. However, there were no significant differences between the 2 groups on the angiographic and functional outcomes both at discharge and 90 days after stroke.

Relationship between Coronary VH-IVUS Plaque Characteristics and CTRP9, SAA, and Hcy in Patients with Coronary Heart Disease.

Coronary heart disease is a common disease threatening human health. In recent years, the incidence of coronary heart disease in China has only increased. It is the most common type of organ disease caused by coronary atherosclerosis, which is observed in the aorta, carotid artery, and femoral artery. The main clinical treatments for coronary heart disease include coronary artery bypass grafting and drug treatment. To investigate the relationship of serum adipocytokine C1q/tumor necrosis factor-related protein 9 (CTRP9), amyloid A (SAA), and plasma homocysteine (Hcy) with coronary artery plaque characteristics in patients with coronary heart disease. Overall, 143 patients with coronary heart disease admitted to our hospital are selected as research participants. The proportion of plaque necrosis core volume is higher in group A than in group B, and the differences are statistically significant (P < 0.05). In group A, necrotic core volume percentage is negatively correlated with CTRP9 levels and positively correlated with SAA and Hcy levels (P < 0.05). Logistic regression analysis revealed that increased systolic blood pressure, increased number of coronary artery lesions, decreased CTRP9 levels, and increased Hcy levels are independent risk factors for thin fibrous cap atherosclerosis in patients with coronary heart disease (P < 0.05). Decreased CTRP9 levels and increased Hcy levels are independent risk factors for coronary heart disease patients with thin fibrous cap atherosclerosis.

Efficacy and Safety of Ultrasound Guided-Deep Serratus Anterior Plane Blockade With Different Doses of Dexmedetomidine for Women Undergoing Modified Radical Mastectomy: A Randomized Controlled Trial.

BACKGROUND: Ultrasound guided-deep serratus anterior plane block (USG-DSAPB) has been used for pain management of patients undergoing modified radical mastectomy (MRM), but evidence supporting their adjuvant analgesic benefits is limited. We explored the efficacy and safety of preemptive use of ropivacaine combined with different doses of dexmedetomidine (DEX) in USG-DSAPB for patients undergoing MRM. METHODS: Ninety-five female patients undergoing unilateral MRM were allocated randomly to two groups. Group RD1 had 20 mL of 0.5% ropivacaine with 5 mg of dexamethasone and 0.5 µg·kg-1 DEX in USG-DSAPB. Group RD2 had 20 mL of 0.5% ropivacaine with 5 mg of dexamethasone and 1 µg·kg-1 DEX in USG-DSAPB. The primary outcome was sufentanil consumption 72 h after USG-DSAPB. Secondary outcomes were: postoperative pain scores and level of sedation; intraoperative hemodynamics; duration of post-anesthesia care unit (PACU) stay; prevalence of moderate-to-severe pain; one-time puncture success; procedure time of blockade; time to first rescue analgesia; requirement of rescue analgesia; satisfaction scores of patients and surgeons; duration of hospital stay; adverse events; prevalence of chronic pain; quality of postoperative functional recovery. RESULTS: Compared with the RD1 group, the visual analog scale score for coughing was significantly lower at 4, 8, 12 h and sufentanil consumption was significantly lower at 4, 8, 12, 24, and 48 h after surgery in the RD2 group (P < 0.05). The time to first rescue analgesia was significantly longer in the RD2 group (P < 0.05). The requirement for rescue analgesia was significantly higher in the RD1 group (P < 0.05). The prevalence of moderate-to-severe pain, number of patients using vasoactive agents, duration of PACU stay, as well as consumption of propofol, remifentanil, and DEX were significantly lower in the RD2 group (P < 0.05). There were no significant differences between the two groups with respect to one-time puncture success, procedure time of blockade, total dermatomal spread, satisfaction scores of patients and surgeons, postoperative complications, duration of hospital stay, 40-item Quality of Recovery questionnaire (QoR-40) score, or prevalence of chronic pain (P > 0.05). CONCLUSIONS: We discovered that 1 µg·kg-1 (not 0.5 µg·kg-1) DEX combined with 20 mL of 0.5% ropivacaine and 5 mg of dexamethasone in USG-DSAPB could provide superior postoperative analgesia for patients undergoing MRM. However, the quality of postoperative functional recovery and prevalence of chronic pain were similar.Clinical Trial Registration: http://www.chictr.org.cn/showproj.aspx?proj=54929, identifier: ChiCTR2000033685.

Rapid color-fading colorimetric sensing of Hg in environmental samples: regulation mechanism from DNA dimension.

It was found that dimension change of aptamer DNA significantly weakened the mimicking activity of gold nanozyme, which was contrary to previous research. Based on this, a rapid colorimetric method for the detection of low concentrations of mercury in environmental media was fabricated. It was observed that 40 nM Hg2+ causes color changes in solution. The detection limit of absorbance measurements was estimated to be 9.3 × 10-11 M. The assay was fast and could complete a single test in half an hour. The detection results for real environment samples confirmed the reliability of the colorimetric analysis in practical application. The proposed assay provides an alternative method for real-time monitoring of mercury in the environment. In particular, the charge effect on the affinity of nanozyme consummated the DNA regulation mechanism for the simulated enzyme activity.