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1.
Allergy Asthma Clin Immunol ; 18(1): 12, 2022 Feb 17.
Artículo en Inglés | MEDLINE | ID: mdl-35177102

RESUMEN

BACKGROUND: Epidemiological studies have revealed a link between atopic dermatitis (AD) and asthma. AS1517499, a selective signal transducer and activation of transcription 6 (STAT6) inhibitor, has been shown to effectively block this connection. In this study, we further explored the underlying mechanism by constructing an AD mouse model. METHODS: Female BALB/c mice were randomly divided into four groups (n = 10/group). The AD mouse model was established by 2,4-dinitrochlorobenzene induction with repeated ovalbumin challenge. AS1517499 and corn oil were used as treatment interventions. The features of airway inflammation, remodeling, and hyperactivity were analyzed. RESULTS: Active use of AS1517499 in AD mice effectively reduced Th2-related cytokine levels, alleviated airway eosinophil and lymphocyte infiltration, and regulated GATA3/Foxp3 levels and subepithelial collagen deposition. These changes might be due to specific blockade of the STAT6 signaling pathway. CONCLUSION: AS1517499 could partially block the association between AD and asthma by specifically inhibiting the STAT6 signaling pathway.

2.
Allergol Immunopathol (Madr) ; 49(3): 100-107, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33938194

RESUMEN

BACKGROUND: In this study, we investigated the relationship between long-chain non-coding RNAs (lncRNAs) and respiratory syncytial virus (RSV)-exacerbated asthma. METHODS: Transcriptome microarray was used to detect differentially expressed lncRNAs in dendritic cells (DCs) co-cultured with RSV-infected human airway epithelial cells and DCs infected with RSV. The identified downregulation of lncRNA n337374 was validated using fluorescence RT-qPCR. LncRNA n337374-overexpressing DCs and RSV-exacerbated asthmatic mouse models were established. Airway hyper-reactivity and bronchoalveolar lavage fluid (BALF) were examined, and pathological changes in lung tissues were observed in mice. Surface molecules in DCs were detected by flow cytometry and RT-qPCR and the expression of CD86 and mitogen-activated protein kinases was determined by western blot. RESULTS: In an RSV-exacerbated asthmatic mouse model, the airway wall was thickened, luminal stenosis was observed, a large number of inflammatory cells were infiltrated in the lung tissue, lung function was impaired, and counts of inflammatory cells in the BALF were increased. The overexpression of lncRNA n337374 ameliorated these pathological changes and improved impaired lung function and inflammation in an asthmatic mouse model. In DCs co-cultured with RSV-infected human airway epithelial cells, CD86 expression was promoted and ERK was markedly phosphorylated. When lncRNA n337374-overexpressing DCs were used in the co-cultures, the expression of CD86 and phosphorylated ERK was decreased. CONCLUSION: The results suggest that lncRNA n337374 overexpression may suppress DC maturation by downregulating the CD86 and ERK pathway, subsequently relieving the symptoms of RSV-induced asthma. LncRNA n337374 may be a promising target in the treatment of RSV infection-induced asthma.


Asunto(s)
Asma/metabolismo , Antígeno B7-2/metabolismo , Células Dendríticas/metabolismo , Sistema de Señalización de MAP Quinasas , ARN Largo no Codificante/metabolismo , Infecciones por Virus Sincitial Respiratorio/complicaciones , Animales , Asma/virología , Líquido del Lavado Bronquioalveolar/citología , Fenómenos Fisiológicos Celulares , Células Dendríticas/fisiología , Células Dendríticas/virología , Modelos Animales de Enfermedad , Regulación hacia Abajo , Femenino , Perfilación de la Expresión Génica , Humanos , Lentivirus/metabolismo , Pulmón/patología , Ratones , Ratones Endogámicos BALB C , Fosforilación , ARN Largo no Codificante/análisis , Hipersensibilidad Respiratoria/diagnóstico , Virus Sincitial Respiratorio Humano , Regulación hacia Arriba
3.
J Nanobiotechnology ; 18(1): 138, 2020 Sep 29.
Artículo en Inglés | MEDLINE | ID: mdl-32993684

RESUMEN

BACKGROUND: Neutrophil-based drug delivery system possesses excellent advantages in targeting at tumour because neutrophils are easily recruited by chemotactic factor in tumor microenvironment. Herein, we developed a novel tactic of multistage neutrophils-based nanoparticle delivery system for promoting photothermal therapy (PTT) of lung cancer. RESULTS: Au nanorod (AuNR) was successfully modified with bovine serum albumin (AuNRB) and further conjugated with RGD (AuNRBR), followed by neutrophil internalisation to obtain neutrophils-based delivery system (AuNRBR/N). The engineered neutrophils efficiently migrated across the epithelial cells due to inflammatory signal. They exhibited better toxicity against Lewis cells with laser irradiation in vitro. Moreover, AuNRBR/N showed significantly more targetability to tumour tissue compared with cell carrier-free AuNRBR, as demonstrated in Lewis tumour-bearing mice. The enhanced tumour homing efficiency of AuNRBR/N together with subsequently released AuNRBR from the neutrophils was favourable for further deep tissue diffusion and contributed to the inhibition of the tumour growth in PTT and improved survival rate (over 120 days). CONCLUSIONS: Overall results illustrated that the design of cell-based nanoparticle delivery system for PTT of cancer is promising.


Asunto(s)
Sistemas de Liberación de Medicamentos/métodos , Nanopartículas/uso terapéutico , Neutrófilos/efectos de los fármacos , Terapia Fototérmica/métodos , Animales , Línea Celular Tumoral , Terapia Combinada , Femenino , Oro/farmacología , Humanos , Ratones , Ratones Endogámicos C57BL , Nanotubos , Fotoquimioterapia/métodos , Fototerapia , Tasa de Supervivencia
4.
Aging (Albany NY) ; 12(20): 20198-20211, 2020 08 27.
Artículo en Inglés | MEDLINE | ID: mdl-32852284

RESUMEN

We aimed to elucidate the roles of the long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3)/microRNA-7b (miR-7b)/NLR pyrin domain containing 3 (NLRP3) axis in lipopolysaccharide (LPS)-induced acute lung injury (ALI). Mouse alveolar macrophage NR8383 and mice were administrated with LPS to establish ALI models in vitro and in vivo. NLRP3 was silenced while miR-7b was overexpressed in LPS-induced NR8383 cell model of ALI. The interleukin-18 (IL-18) and IL-1ß, as well as caspase-1, tumor necrosis factor-α (TNF-α) and IL-6 protein levels were assayed. To further investigate the underlying mechanisms of NLRP3 in ALI, lncRNA MEG3 was silenced and miR-7b was overexpressed in LPS-induced NR8383 cell model of ALI, after which in vivo experiments were performed for further verification. NLRP3 was highly expressed in LPS-induced NR8383 cell model of ALI. Silencing NLRP3 or overexpressing miR-7b inhibited IL-18 and IL-1ß, as well as caspase-1, TNF-α and IL-6. LncRNA MEG3 could sponge miR-7b, and lncRNA MEG3 silencing or miR-7b overexpression downregulates NLRP3 expression, thus reducing IL-18 and IL-1ß, as well as caspase-1, TNF-α and IL-6 levels. The in vivo experiments further confirmed the aforementioned findings. Silencing lncRNA MEG3 augments miR-7b binding to NLRP3 and downregulates NLRP3 expression, which ultimately improves LPS-induced ALI.


Asunto(s)
Lesión Pulmonar Aguda/prevención & control , Inflamasomas/metabolismo , Pulmón/metabolismo , Macrófagos Alveolares/metabolismo , MicroARNs/metabolismo , Proteína con Dominio Pirina 3 de la Familia NLR/metabolismo , Interferencia de ARN , ARN Largo no Codificante/metabolismo , Lesión Pulmonar Aguda/inducido químicamente , Lesión Pulmonar Aguda/genética , Lesión Pulmonar Aguda/metabolismo , Animales , Caspasa 1/genética , Caspasa 1/metabolismo , Línea Celular , Citocinas/genética , Citocinas/metabolismo , Modelos Animales de Enfermedad , Regulación de la Expresión Génica , Inflamasomas/genética , Lipopolisacáridos , Pulmón/patología , Macrófagos Alveolares/patología , Masculino , Ratones Endogámicos C57BL , MicroARNs/genética , Proteína con Dominio Pirina 3 de la Familia NLR/genética , ARN Largo no Codificante/genética , Transducción de Señal
5.
Cell Physiol Biochem ; 41(4): 1370-1382, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28427045

RESUMEN

BACKGROUND: Non-small-cell lung cancer (NSCLC) is a deadly cancer with high mortality rate. Drug resistance represents a main obstacle in NSCLC treatment. High mobility group box-1 (HMGB1) protein promotes drug resistance in NSCLC cells by activating protective autophagy. METHODS: In the current study, we investigated the regulatory role of microRNA-142-3p (miR-142-3p) in HMGB1-mediated autophagy of NSCLC cells and its impact on drug resistance of NSCLC in vitro and in vivo. HMGB1 was identified as a putative target gene of miR-142-3p by in silico analysis. Our luciferase reporter assay results confirmed that miR-142-3p directly targets the 3'-UTR of HMGB1 in NSCLC cells. RESULTS: MiR-142-3p overexpression suppressed while miR-142-3p knockdown increased HMGB1 mRNA and protein expression. Starvation induced HMGB1 expression and activated autophagy in NSCLC cells. The starvation-induced autophagy was inhibited by miR-142-3p overexpression or HMGB1 knockdown. Moreover, miR-142-3p overexpression or HMGB1 knockdown increased PI3K, Akt, and mTOR phosphorylation. Inhibition of PI3K or mTOR restored starvation-induced autophagy inhibited by miR-142-3p overexpression or HMGB1 knockdown. CONCLUSIONS: These results demonstrated that miR-142-3p regulates starvation-induced autophagy of NSCLC cells by directly downregulating HMGB1 and subsequently activating the PI3K/Akt/mTOR pathway. Further, miR-142-3p overexpression inhibited anticancer drug-induced autophagy and increased chemo-sensitivity of NSCLC in vitro and in vivo. These findings shed light on the therapeutic potential of miR-142-3p in combating acquired NSCLC chemo-resistance.


Asunto(s)
Autofagia , Carcinoma de Pulmón de Células no Pequeñas/metabolismo , Resistencia a Antineoplásicos , Proteína HMGB1/metabolismo , Neoplasias Pulmonares/metabolismo , MicroARNs/biosíntesis , Proteínas de Neoplasias/metabolismo , ARN Neoplásico/biosíntesis , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Carcinoma de Pulmón de Células no Pequeñas/genética , Carcinoma de Pulmón de Células no Pequeñas/patología , Línea Celular Tumoral , Proteína HMGB1/genética , Humanos , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patología , MicroARNs/genética , Proteínas de Neoplasias/genética , ARN Neoplásico/genética
7.
Inflammation ; 40(2): 546-554, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28063089

RESUMEN

In this paper, we aimed to explore the potential mechanism underlying atopic dermatitis (AD) and its association with asthma. The BALB/c mice were randomly assigned to three groups, including the vehicle control (VD) group, the AD group, and the treatment (TR) group. The AD mice model was successfully constructed in the AD and TR group. The dermatitis severity scores and skin lesions were significantly increased in AD mice after DNCB application. Airway responsiveness in the AD group was significantly higher than in the TR group. The number of inflammatory cells was increased in skin lesions and bronchoalveolar lavage fluid (BALF) of AD mice. The levels of IL-4, IL-5, IFN-γ, and OVA-IgE in BALF supernatants of mice in the AD group were higher than those in the VC group. All the changes in AD mice were decreased by tacrolimus. These results indicate that AD may be a significant risk factor for atopic asthma development.


Asunto(s)
Asma/etiología , Dermatitis Atópica/complicaciones , Animales , Líquido del Lavado Bronquioalveolar/inmunología , Dinitroclorobenceno/farmacología , Eccema , Ratones , Ratones Endogámicos BALB C , Tacrolimus/farmacología
8.
J Cell Commun Signal ; 11(3): 219-226, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28101818

RESUMEN

Autophagy, a catabolic process by which cytoplasmic components are degraded in lysosomes, plays an important role in the maintenance of cellular homeostasis. Dysregulation of autophagy is associated with several diseases. However, few studies have addressed the role of autophagy in the lung, and its role in lung diseases remains unclear. In the present study, we examined the effect of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on autophagy in A549 cells and explored the underlying mechanisms. We showed that TRAIL promoted autophagosome formation, as detected by the levels of LC3-II, and its effect on promoting autophagy was dependent on the expression of the autophagy related genes (ATGs) Atg5, Atg7, and beclin-1. TRAIL-induced ATG expression was attenuated by JNK silencing or treatment with the JNK inhibitor SP600125, indicating the involvement of the JNK pathway. Crosstalk between autophagy and apoptosis was demonstrated by silencing the autophagy related genes Atg5, Atg7, and beclin-1, and the dependence of TRAIL-induced apoptosis on autophagy-related gene expression. Taken together, our results indicate that TRAIL promotes autophagy in A549 cells via a mechanism involving the modulation of ATG expression through the JNK pathway. Inhibition of autophagy enhanced TRAIL-induced cell proliferative inhibition and apoptosis in A549 cells.

9.
Artif Organs ; 40(12): E253-E265, 2016 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-27911030

RESUMEN

We aimed to investigate whether acellular endocardium can be used as a useful biomaterial for the intima of engineered small-caliber vascular grafts. Fresh endocardium was harvested from the swine left atrium and was decellularized by digestion with the decellularization solution of Triton X-100 and SDS containing DNase I and RNase A. Surface morphological characteristics and Young's modulus were evaluated. To analyze the effect of mechanical characteristics on cell adhesion, the decellularized endocardium was stiffened with 2.5% glutaraldehyde. Small-caliber vascular grafts were constructed using decellularized endocardium treated with or without glutaraldehyde as the intima. CD34+ cells were seeded onto the luminal surface of the vascular grafts and linked to bioreactors that simulate a pulsatile blood stream. Acellular endocardium had distinct surface morphological characteristics, which were quite different from those of other materials. The compliance of acellular endocardium was higher than that of other materials tested by Young's modulus. CD34+ cells formed a monolayer structure and adhered to the inner face of the acellular endocardium. The glutaraldehyde treatment stiffened the acellular endocardium but had little impact on the surface morphological characteristics or static adhesiveness of the cells. Data from the bioreactor study showed that the detachment of the cells from the surface of glutaraldehyde-treated acellular endocardium increased dramatically when the pressure was equal or higher than 40 mm Hg, while the cells on the untreated acellular endocardium remained well and formed confluent monolayers and tight junctions under the same pressure. Acellular endocardium has distinct structures and mechanical characteristics that are beneficial for CD34+ cell adhesion and retention under dynamic fluid perfusion. Thus, it can be used as a useful biomaterial for the construction of the intima of engineered small-caliber vascular grafts.


Asunto(s)
Materiales Biocompatibles/química , Prótesis Vascular , Endocardio/química , Andamios del Tejido/química , Túnica Íntima/química , Animales , Antígenos CD34/análisis , Bioprótesis , Células de la Médula Ósea/citología , Arterias Carótidas/fisiología , Adhesión Celular , Proliferación Celular , Células Cultivadas , Colágeno/química , Módulo de Elasticidad , Glutaral/química , Nanofibras/química , Poliuretanos/química , Porosidad , Porcinos
10.
Mol Med Rep ; 12(4): 5568-72, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26151622

RESUMEN

Non­small­cell lung cancer (NSCLC) accounts for ~85% of all lung cancer cases, with a 5­year survival rate of <15%. 1­O­acetylbritannilactone (ABL), a natural chemical component obtained from inula britannica, a Chinese traditional medicine, has been demonstrated to have anticancer activity. In the present study, the antiproliferative and proapoptotic abilities of ABL alone or in combination with gemcitabine in a human NSCLC cell line were investigated. A549 cells were treated in vitro with ABL, gemcitabine, and a combination of ABL and gemcitabine for 72 h. The results demonstrated that ABL and gemcitabine inhibited cell growth and induced apoptosis of A549 cells. These effects were more potent following the combination of ABL and gemcitabine treatment than either agent alone. Furthermore, the signal transduction analysis revealed nuclear factor (NF)­κB expression was significantly decreased by ABL and the combination treatment. The inhibitor nuclear factor κBα (IκBα) and Bax levels were upregulated whereas Bcl­2 was substantially downregulated following treatment. The present findings suggest that ABL combined with gemcitabine elicits potent apoptosis of lung cancer cells and therefore, ABL has the potential to be developed as a chemotherapeutic agent.


Asunto(s)
Antineoplásicos/farmacología , Desoxicitidina/análogos & derivados , Células Epiteliales/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica , Lactonas/farmacología , Apoptosis/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Desoxicitidina/farmacología , Combinación de Medicamentos , Sinergismo Farmacológico , Células Epiteliales/metabolismo , Células Epiteliales/patología , Humanos , Proteínas I-kappa B/agonistas , Proteínas I-kappa B/genética , Proteínas I-kappa B/metabolismo , Inhibidor NF-kappaB alfa , FN-kappa B/antagonistas & inhibidores , FN-kappa B/genética , FN-kappa B/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Mucosa Respiratoria/efectos de los fármacos , Mucosa Respiratoria/metabolismo , Mucosa Respiratoria/patología , Transducción de Señal , Proteína X Asociada a bcl-2/agonistas , Proteína X Asociada a bcl-2/genética , Proteína X Asociada a bcl-2/metabolismo , Gemcitabina
11.
Mol Med Rep ; 10(3): 1400-8, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24938646

RESUMEN

This study investigated the effect of Panax notoginseng saponins (PNS) on acute lung injury (ALI) induced by oleic acid (OA) and lipopolysaccharide (LPS). A total of 28 Wistar rats were divided into four groups: sham; sham + PNS; OA­LPS­induced ALI and ALI + PNS. Lung tissue histology, lung wet­to­dry (W/D) weight ratio, extravascular lung water (EVLW) and epithelial sodium channel α (αENaC) mRNA and protein expression were examined. In addition, levels of inflammatory cytokines, including tumor necrosis factor α (TNF­α), interleukin (IL)­6 and IL­10, as well as total leukocyte and neutrophil counts, were analyzed in rat bronchoalveolar lavage fluid (BALF) and serum. ALI + PNS rats were observed to exhibit significantly lower pulmonary parenchymal damage and EVLW compared with ALI rats. Furthermore, total leukocyte and neutrophil counts, and levels of inflammatory cytokines were significantly decreased following PNS administration in ALI rats. In addition, the decrease in αENaC mRNA and protein expression observed in the lung tissue of ALI rats was partially restored following PNS treatment. PNS treatment was demonstrated to ameliorate OA­LPS­induced ALI, potentially through restoration of αENaC mRNA and protein expression and through PNS­induced anti­inflammatory effects.


Asunto(s)
Lesión Pulmonar Aguda/tratamiento farmacológico , Antiinflamatorios/farmacología , Lipopolisacáridos/efectos adversos , Ácido Oléico/efectos adversos , Panax notoginseng/química , Saponinas/farmacología , Lesión Pulmonar Aguda/inducido químicamente , Animales , Líquido del Lavado Bronquioalveolar , Modelos Animales de Enfermedad , Canales Epiteliales de Sodio/genética , Canales Epiteliales de Sodio/metabolismo , Interleucina-10/sangre , Interleucina-6/sangre , Leucocitos/citología , Leucocitos/efectos de los fármacos , Lipopolisacáridos/administración & dosificación , Pulmón/efectos de los fármacos , Pulmón/patología , Masculino , Neutrófilos/citología , Neutrófilos/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Factor de Necrosis Tumoral alfa/sangre
12.
Immunology ; 142(1): 78-88, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-24329550

RESUMEN

Aspergillus fumigatus is often associated in asthmatic patients with the exacerbation of asthma symptoms. The pathomechanism of this phenomenon has not been fully understood. Here, we evaluated the immunological mechanisms and the role of the prostaglandin D2 / Chemoattractant Receptor-Homologous Molecule Expressed on Th2 Cells (CRTH2) pathway in the development of Aspergillus-associated asthma exacerbation. We studied the effects of A. fumigatus on airway inflammation and bronchial hyper-responsiveness in a rat model of chronic asthma. Inhalation delivery of A. fumigatus conidia increased the airway eosinophilia and bronchial hyper-responsiveness in ovalbumin-sensitized, challenged rats. These changes were associated with prostaglandin D2 synthesis and CRTH2 expression in the lungs. Direct inflammation occurred in ovalbumin-sensitized, challenged animals, whereas pre-treatment with an antagonist against CRTH2 nearly completely eliminated the A. fumigatus-induced worsening of airway eosinophilia and bronchial hyper-responsiveness. Our data demonstrate that production of prostaglandin D2 followed by eosinophil recruitment into the airways via a CRTH2 receptor are the major pathogenic factors responsible for the A. fumigatus-induced enhancement of airway inflammation and responsiveness.


Asunto(s)
Aspergillus fumigatus/patogenicidad , Asma/metabolismo , Hiperreactividad Bronquial/metabolismo , Pulmón/metabolismo , Prostaglandina D2/metabolismo , Aspergilosis Pulmonar/metabolismo , Receptores Inmunológicos/metabolismo , Receptores de Prostaglandina/metabolismo , Animales , Antiinflamatorios/farmacología , Asma/inmunología , Asma/microbiología , Asma/fisiopatología , Hiperreactividad Bronquial/inmunología , Hiperreactividad Bronquial/microbiología , Hiperreactividad Bronquial/fisiopatología , Modelos Animales de Enfermedad , Eosinófilos/inmunología , Eosinófilos/metabolismo , Pulmón/efectos de los fármacos , Pulmón/inmunología , Pulmón/microbiología , Pulmón/fisiopatología , Masculino , Ovalbúmina , Aspergilosis Pulmonar/inmunología , Aspergilosis Pulmonar/microbiología , Aspergilosis Pulmonar/fisiopatología , Eosinofilia Pulmonar/inmunología , Eosinofilia Pulmonar/metabolismo , Eosinofilia Pulmonar/microbiología , Ratas , Ratas Wistar , Receptores Inmunológicos/antagonistas & inhibidores , Receptores de Prostaglandina/antagonistas & inhibidores , Transducción de Señal
13.
Am J Respir Cell Mol Biol ; 45(1): 95-100, 2011 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20855649

RESUMEN

The pathogenesis of allergic asthma is characterized by airway inflammation, eosinophilia, and airway hyperresponsiveness. In the present study, we investigated the anti-inflammatory effects of mastic, obtained from the stem and the leaves of Pistacia lentiscus trees, on allergic asthma. In an ovalbumin-induced mouse asthma model, mastic significantly inhibited eosinophilia, while reducing airway hyperresponsiveness and suppressing the production of inflammatory cytokines (IL-5 and IL-13) as well as chemokines (eotaxin, eotaxin2, and regulated upon activation, normal T-cell expressed and secreted) in bronchoalveolar lavage fluid. Moreover, mastic potently inhibited eotaxin-induced eosinophil chemotaxis in vitro without influencing eotaxin receptor, chemokine receptor 3, expression. These results suggest that mastic may contribute to the treatment of inflammatory diseases.


Asunto(s)
Asma/tratamiento farmacológico , Eosinófilos/metabolismo , Pistacia/química , Hojas de la Planta/química , Tallos de la Planta/química , Resinas de Plantas/farmacología , Animales , Asma/patología , Lavado Broncoalveolar , Quimiotaxis/efectos de los fármacos , Citocinas/metabolismo , Modelos Animales de Enfermedad , Eosinófilos/patología , Resina Mástique , Ratones , Ratones Endogámicos BALB C , Eosinofilia Pulmonar/tratamiento farmacológico , Eosinofilia Pulmonar/metabolismo , Eosinofilia Pulmonar/patología
14.
Immunol Cell Biol ; 89(2): 231-8, 2011 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-20603637

RESUMEN

The respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections in children, the elderly and in people who are immune suppressed, and is also the cause for the development of asthma primarily in infants. However, the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aim of this study was to examine the influence of RSV-infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells (mDCs).We found that the exposure of primary rat airway epithelial cells (PRAECs) to RSV induced a rapid (6 h), high (12 h) and persistent (18 h) increase in thymic stromal lymphopoietin (TSLP) mRNA compared with untreated PRAECs. TSLP protein expression was also enhanced by RSV infection. Functional maturation of mDCs was induced by RSV-treated PRAECs, as shown by their enhanced levels of OX40L and thymus- and activation-regulated chemokine (TARC) mRNAs, which increased the expressions of major histocompatibility complex II (MHCII) and CD86 costimulatory molecules and promoted enhanced T-cell proliferation in mixed lymphocyte reactions. These activities were inhibited in cocultures with RSV-infected RTECs (rat tracheal epithelial cells, an immortalized cell strain) that had been pretreated with TSLP-targeted small interfering RNA. These results suggest that RSV can induce epithelial cells to produce TSLP, which in turn promotes the maturation of mDCs that might support Th2 cell polarization.


Asunto(s)
Citocinas/metabolismo , Células Dendríticas/inmunología , Células Epiteliales/virología , Células Mieloides/citología , Virus Sincitiales Respiratorios/fisiología , Tráquea/citología , Animales , Biomarcadores , Diferenciación Celular , Proliferación Celular , Células Cultivadas , Técnicas de Cocultivo , Citocinas/genética , Células Dendríticas/citología , Ratas , Ratas Wistar , Infecciones por Virus Sincitial Respiratorio/inmunología , Infecciones por Virus Sincitial Respiratorio/virología , Células TH1/citología , Células TH1/inmunología , Células Th2/citología , Células Th2/inmunología , Receptor Toll-Like 3/metabolismo , Regulación hacia Arriba , Linfopoyetina del Estroma Tímico
15.
Respirology ; 14(3): 360-70, 2009 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-19207119

RESUMEN

BACKGROUND AND OBJECTIVE: Epidemiological evidence indicates a close link between exposure to fungi and deterioration of asthma. However, the role of fungi as an exogenous precipitant for initiation and progression of asthma has been incompletely explored. In this study, the effects of Aspergillus fumigatus exposure on airway inflammation and remodelling in a rat model of chronic asthma were investigated. METHODS: The rat model of chronic asthma was established by systemic sensitization and repeated challenge with ovalbumin (OVA). The asthmatic rats were exposed to chronic intranasal inhalation of A. fumigatus spores. Changes in airway inflammation, remodelling and BHR were measured after exposure to the fungus. RESULTS: Chronic inhalation of A. fumigatus spores elevated the production of T helper 2 (Th2) cytokines, increased the concentration of total serum IgE, and resulted in the recruitment of eosinophils and lymphocyte infiltration into the airways of asthmatic rats. Goblet cell hyperplasia, mucus hyperproduction and subepithelial collagen deposition were also induced by inhalation of the fungus. The remodelling changes induced by inhalation of the fungus paralleled the changes in BHR in this rat model of asthma. CONCLUSIONS: Chronic exposure to A. fumigatus aggravated Th2 airway inflammation, promoted airway remodelling and increased BHR in OVA-sensitized and -challenged rats.


Asunto(s)
Aspergillus fumigatus , Asma/microbiología , Asma/patología , Neumonía/microbiología , Neumonía/patología , Administración Intranasal , Animales , Aspergillus fumigatus/patogenicidad , Asma/inducido químicamente , Movimiento Celular , Enfermedad Crónica , Colágeno/metabolismo , Citocinas/metabolismo , Modelos Animales de Enfermedad , Eosinófilos/patología , Inmunoglobulina E/sangre , Masculino , Ovalbúmina , Neumonía/metabolismo , Ratas , Ratas Wistar , Hipersensibilidad Respiratoria/metabolismo , Hipersensibilidad Respiratoria/microbiología , Hipersensibilidad Respiratoria/patología , Esporas Fúngicas , Células Th2/metabolismo , Células Th2/patología
16.
Zhonghua Nei Ke Za Zhi ; 43(10): 764-8, 2004 Oct.
Artículo en Chino | MEDLINE | ID: mdl-15631831

RESUMEN

OBJECTIVE: A case of primary pigmented nodular adrenal disease (PPNAD) was first diagnosed in Ruijin Hospital, Shanghai, China and molecular genetic research was then carried on the proband and his family members. METHODS: History and laboratory tests were routinely taken. Liddle's test, adrenal CT and pituitary magnetic resonance imaging were also carried out. Complete family history was obtained and eight of the family members donated their blood for DNA extraction. Polymerase chain reaction was done on all the exons of PRKAR1A gene and the product of the reaction was sequenced with ABI 3700. The right adrenal of the patient was then resected, part of the tissue was preserved in liquid nitrogen for DNA/RNA extraction and the remaining sent to Department of Pathology. RESULTS: The patient presented an atypical appearance of Cushing's syndrome. His father had a typical history of cardiac myoma. Cortisone level could not be refrained in Liddle's test for the patient. Imaging examination presented a nodular adrenal and a full pituitary. A novel mutation of PRKAR1A-S147N was found in both the patient's and his father's gene. CONCLUSIONS: This is the first patient diagnosed as PPNAD based on his clinical manifestations, laboratory tests and imaging and pathological examinations. According to the history of his father and the results of molecular genetic analysis, the diagnosis of Carney complex can be established on this patient and his father. It is also the first time that this kind of point mutation was found in Chinese people.


Asunto(s)
Enfermedades de la Corteza Suprarrenal/genética , Síndrome de Cushing/genética , Proteínas Quinasas Dependientes de AMP Cíclico/genética , Trastornos de la Pigmentación/genética , Enfermedades de la Corteza Suprarrenal/diagnóstico , Adulto , Secuencia de Bases , Síndrome de Cushing/diagnóstico , Subunidad RIalfa de la Proteína Quinasa Dependiente de AMP Cíclico , Exones/genética , Humanos , Masculino , Linaje , Trastornos de la Pigmentación/diagnóstico , Mutación Puntual
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