Lipids signaling and unsaturation of fatty acids participate in ramie response to submergence stress and hypoxia-responsive gene regulation.

Ramie is a valuable crop that produces high-quality fibers and holds promise in ecological management and potential therapeutic properties. The damage of submergence during the fertile period seriously affects the growth of ramie. This study used transcriptomics and UPLC-QTOF/MS-based lipidomics analysis to reveal the lipids remodeling and stress adaptation mechanism in ramie response to submergence. The results of subcellular distribution showed that lipids in ramie leaf cells mostly aggregate in the inter-chloroplast cytoplasm to form lipid droplets under submergence stress. High-performance thin-layer chromatography (HPTLC) and lipidomics analysis showed that the composition and content of lipids in ramie leaves significantly changed under submergence stress, and the content of fatty acids (FAs) gradually accumulated with the extension of the submergence treatment time. Further analysis revealed that the content of 18:3 (n3) Coenzyme A (C18:3-CoA) increased significantly with the prolongation of submergence stress, and the exogenous addition of C18:3-CoA activated the expression of hypoxia-responsive marker genes such as BnADH1, BnPCO2, BnADH1, and BnPDC1. These results suggest that the ramie lipid metabolism pathways were significantly affected under submergence, and the C18:3-CoA may act directly or indirectly on the hypoxia-responsive genes to activate their transcriptional activities, thereby enhancing the tolerance of ramie to submergence stress.

Comprehensive Analysis of WUSCEL-Related Homeobox Gene Family in Ramie (Boehmeria nivea) Indicates Its Potential Role in Adventitious Root Development.

A WUSCHEL-related homeobox (WOX) gene family has been implicated in promoting vegetative organs to embryonic transition and maintaining plant embryonic stem cell identity. Using genome-wide analysis, we identified 17 candidates, WOX genes in ramie (Boehmeria nivea). The genes (BnWOX) showed highly conserved homeodomain regions typical of WOX. Based on phylogenetic analysis, they were classified into three distinct groups: modern, intermediate, and ancient clades. The genes displayed 65% and 35% collinearities with their Arabidopsis thaliana and Oryza sativa ortholog, respectively, and exhibited similar motifs, suggesting similar functions. Furthermore, four segmental duplications (BnWOX10/14, BnWOX13A/13B, BnWOX9A/9B, and BnWOX6A/Maker00021031) and a tandem-duplicated pair (BnWOX5/7) among the putative ramie WOX genes were obtained, suggesting that whole-genome duplication (WGD) played a role in WOX gene expansion. Expression profiling analysis of the genes in the bud, leaf, stem, and root of the stem cuttings revealed higher expression levels of BnWOX10 and BnWOX14 in the stem and root and lower in the leaf consistent with the qRT-PCR analysis, suggesting their direct roles in ramie root formation. Analysis of the rooting characteristics and expression in the stem cuttings of sixty-seven different ramie genetic resources showed a possible involvement of BnWOX14 in the adventitious rooting of ramie. Thus, this study provides valuable information on ramie WOX genes and lays the foundation for further research.

A further study on coherence between virtual signal and physical signals in remote acoustic sensing (L).

A recent paper by Zhang, Wang, Duan, Tao, Zou, and Qiu [(2022). J. Acoust. Soc. Am. 152, 2840-2848] proposed that the average distance between physical microphones in remote acoustic sensing should be no larger than half the wavelength to ensure the coherence between the virtual signal and physical signals no less than 0.9 in complex sound fields. In this letter, the effects of the sound sources' distance on the coherence between the virtual signal and physical signals are further investigated, and it is found that the required average distance among physical microphones becomes smaller when sound sources are closer to the microphones.

Active control of sound transmission through a floor-level slit.

The floor-level slit between the door and the floor is one weak point in building noise insulation. In this paper, an active noise control system is proposed to reduce the sound transmission through a floor-level slit with evenly distributed secondary sources on its top boundary. The system performance is first investigated based on the analytical and numerical models, and simulation results indicate a decrease in active control performance with increasing frequency. The upper limit frequency of 10 dB effective control increases with a higher number of secondary sources, and the corresponding wavelength of the upper limit frequency is approximately the interval between the secondary sources when a plane wave is incident normally. Although the upper limit frequency decreases with the slit height, it approaches a constant when the slit height becomes significantly smaller than the wavelength in the incident sound. The experimental results based on a typical floor-level door slit support the findings in the numerical simulations. For a slit with a width of 0.9 m and a height of 0.005 m, the upper limit frequency of 10 dB noise reduction can reach up to 2830 Hz when ten secondary sources are employed in the experiments.

Genome-Wide Identification and Expression Analysis of BnPP2C Gene Family in Response to Multiple Stresses in Ramie (Boehmeria nivea L.).

The protein phosphatase 2C (PP2C), a key regulator of the ABA signaling pathway, plays important roles in plant growth and development, hormone signaling, and abiotic stress response. Although the PP2C gene family has been identified in many species, systematic analysis was still relatively lacking in ramie (Boehmeria nivea L.). In the present study, we identified 63 BnPP2C genes from the ramie genome, using bioinformatics analysis, and classified them into 12 subfamilies, and this classification was consistently supported by their gene structures and conserved motifs. In addition, we observed that the functional differentiation of the BnPP2C family of genes was restricted and that fragment replication played a major role in the amplification of the BnPP2C gene family. The promoter cis-regulatory elements of BnPP2C genes were mainly involved in light response regulation, phytohormone synthesis, transport and signaling, environmental stress response and plant growth and development regulation. We identified BnPP2C genes with tissue specificity, using ramie transcriptome data from different tissues, in rhizome leaves and bast fibers. The qRT-PCR results showed that the BnPP2C1, BnPP2C26 and BnPP2C27 genes had a strong response to drought, high salt and ABA, and there were a large number of stress-responsive elements in the promoter region of BnPP2C1 and BnPP2C26. The results suggested that BnPP2C1 and BnPP2C26 could be used as the candidate genes for drought and salt tolerance in ramie. These results provide a reference for further studies on the function of the PP2C gene and advance the development of the mechanism of ramie stress response, with a view to providing candidate genes for the molecular breeding of ramie for drought and salt tolerance.

Comparative genome and metabolome analyses uncover the evolution and flavonoid biosynthesis between Apocynum venetum and Apocynum hendersonii.

Apocynum species have great application prospects in textile and phytoremediation of saline soil, are rich in flavonoids, and possess medicinal significance. Here, we report the draft genomes of Apocynum venetum and Apocynum hendersonii, and elucidate their evolutionary relationship. The high synteny and collinearity between the two suggested that they have experienced the same WGD event. Comparative analysis revealed that flavone 3-hydroxylase (ApF3H) and differentially evolved flavonoid 3-O-glucosyl transferase (ApUFGT) genes are critical for determining natural variation in flavonoid biosynthesis between the species. Overexpression of ApF3H-1 enhanced the total flavonoid content and promoted the antioxidant capacity of transformed plants compared to the wild-type. ApUFGT5 and 6 explained the diversification of flavonoids or their derivatives. These data provide biochemical insight and knowledge on the genetic regulation of flavonoid biosynthesis, supporting the adoption of these genes in breeding programs aimed at the multipurpose utilization of the plants.

Genome-Wide Investigation of the NAC Transcription Factor Family in Apocynum venetum Revealed Their Synergistic Roles in Abiotic Stress Response and Trehalose Metabolism.

NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) are one of the most prominent plant-specific TF families and play essential roles in plant growth, development and adaptation to abiotic stress. Although the NAC gene family has been extensively characterized in many species, systematic analysis is still relatively lacking in Apocynum venetum (A. venetum). In this study, 74 AvNAC proteins were identified from the A. venetum genome and were classified into 16 subgroups. This classification was consistently supported by their gene structures, conserved motifs and subcellular localizations. Nucleotide substitution analysis (Ka/Ks) showed the AvNACs to be under the influence of strong purifying selection, and segmental duplication events were found to play the dominant roles in the AvNAC TF family expansion. Cis-elements analysis demonstrated that the light-, stress-, and phytohormone-responsive elements being dominant in the AvNAC promoters, and potential TFs including Dof, BBR-BPC, ERF and MIKC_MADS were visualized in the TF regulatory network. Among these AvNACs, AvNAC58 and AvNAC69 exhibited significant differential expression in response to drought and salt stresses. The protein interaction prediction further confirmed their potential roles in the trehalose metabolism pathway with respect to drought and salt resistance. This study provides a reference for further understanding the functional characteristics of NAC genes in the stress-response mechanism and development of A. venetum.

Transmission loss and directivity of sound transmitted through a slit on ground.

An analytical model is proposed for sound transmission through a slit on a rigid ground based on the modal superposition method to investigate the transmission loss (TL). A simple formula is derived for estimation of the TL for plane waves with and without the ground, which gives a more precise prediction than existing approaches. It is found that a larger slit height generally decreases the TL, except at the resonant frequencies of the slit. The slit width has little effect on the TL at high frequencies, and the slit depth affects the resonant frequencies significantly even though it has little effect on the overall TL. Compared with the same size slit in the free field, the rigid ground reduces the TL at most frequencies, and that reduction is a constant between 3 and 9 dB in the low frequency range. It is also found that the sound transmitted through the slit is almost omnidirectional at low frequencies, while most of the sound energy at high frequencies falls within the range where the long side of the slit is located. The experimental results demonstrate the validity of the analytical model and the findings in numerical simulations.

Genome-Wide Analysis of AP2/ERF Gene Superfamily in Ramie (Boehmeria nivea L.) Revealed Their Synergistic Roles in Regulating Abiotic Stress Resistance and Ramet Development.

AP2/ERF transcription factors (TFs) are one of the largest superfamilies in plants, and play vital roles in growth and response to biotic/abiotic stresses. Although the AP2/ERF family has been extensively characterized in many species, very little is known about this family in ramie (Boehmeria nivea L.). In this study, 138 AP2/ERF TFs were identified from the ramie genome and were grouped into five subfamilies, including the AP2 (19), RAV (5), Soloist (1), ERF (77), and DREB (36). Unique motifs were found in the DREB/ERF subfamily members, implying significance to the AP2/ERF TF functions in these evolutionary branches. Segmental duplication events were found to play predominant roles in the BnAP2/ERF TF family expansion. Light-, stress-, and phytohormone-responsive elements were identified in the promoter region of BnAP2/ERF genes, with abscisic acid response elements (ABRE), methyl jasmonate response elements, and the dehydration response element (DRE) being dominant. The integrated transcriptome and quantitative real-time PCR (qPCR) revealed 12 key BnAP2/ERF genes positively responding to waterlogging. Five of the genes are also involved in ramet development, with two (BnERF-30 and BnERF-32) further showing multifunctional roles. The protein interaction prediction analysis further verified their crosstalk mechanism in coordinating waterlogging resistance and ramet development. Our study provides new insights into the presence of AP2/ERF TFs in ramie, and provides candidate AP2/ERF TFs for further studies on breeding varieties with coupling between water stress tolerance and high yield.

A study on coherence between virtual signal and physical signals in remote acoustic sensing.

Remote acoustic sensing can be used to estimate the error signals in human ears without placing any physical microphones there. In this paper, the coherence between the signals picked up by physical microphones over a sphere surface and the signal obtained at the sphere center is investigated. Based on the multiple channel coherence formulas in the time domain and frequency domain, the relationship between the coherence and the placement of physical microphones is analyzed by numerical simulations first, then the experimental results obtained in a reverberation chamber and a car cabin are presented to verify the simulation results. Finally, a placement of physical microphones for active control of road noise in car cabins is discussed. Both the numerical and experimental results show that an upper limit frequency exists for accurate sound pressure estimation at the center of a sphere with the sound pressure on the sphere surface. For a sufficiently complex sound field such as that in a reverberation room or in a car, half the wavelength of the upper limit frequency is about the average distance among the physical microphones.

Scattering by a rigid sphere of audio sound generated by a parametric array loudspeaker.

This work investigates the scattering by a rigid sphere of audio sound generated by a parametric array loudspeaker (pal). A computationally efficient method utilizing a spherical harmonic expansion is developed to calculate the quasilinear solution of audio sound fields based on both Kuznetsov and Westervelt equations. The accuracy of using the Westervelt equation is examined, and the rigid sphere scattering effects are simulated with the proposed method. It is found the results obtained using the Westervelt equation are inaccurate near the sphere at low frequencies. Contrary to conventional loudspeakers, the directivity of the audio sound generated by a pal severely deteriorates behind a sphere, as the ultrasounds maintaining the directivity of the audio sound are almost completely blocked by the sphere. Instead, the ultrasounds are reflected and generate audio sound on the front side of the sphere. It means that a listener in front of the pal will hear the audio sound scattered back after introducing the sphere as if it is reflected by the sphere. The experiment results are also presented to validate the numerical results.

Improving the performance of an active staggered window with multiple resonant absorbers.

The active noise control (ANC) technique has been applied in staggered windows to improve the noise reduction at low frequencies. The control performance of such a system deteriorates significantly at some frequencies where the secondary source cannot radiate effectively due to the reflection at the boundaries of the staggered window. A resonant absorber consisting of a perforated panel and coiled up tubes is proposed to solve the problem. By designing a combination of different absorbers, a proper sound absorption coefficient is achieved around the ineffective frequency. Numerical simulations show that the active sound power reduction increases by 13.5 dB at the frequency with the absorbers attached on one end of the staggered window, and the overall sound power reduction between 100 and 500 Hz increases from 25.9 to 31.2 dB. Attaching the sound absorbers elsewhere in the upstream of the secondary source, for example, on the side walls of the duct also works. The active sound power reduction at 435 Hz increases by 6.3 dB after attaching the absorbers in the experiments, and the noise reduction increment at the evaluation point is 13.6 dB, which agrees with simulation results and demonstrates the feasibility of the proposed sound absorbers.

Quiet zone generation in an acoustic free field using multiple parametric array loudspeakers.

This paper investigates the feasibility of remotely generating a quiet zone in an acoustic free field using multiple parametric array loudspeakers (PALs). A primary sound field is simulated using point monopoles located randomly in a two-dimensional plane, or three-dimensional (3D) space, whereas the secondary sound field is generated by multiple PALs uniformly distributed around the circumference of a circle sitting on the same plane as the primary sources, or on the surface of a sphere for 3D space. A quiet zone size is defined as the diameter of the maximal circular zone within which the noise reduction is greater than 10 dB. The size of this quiet zone is found to be proportional to 0.19λN for N secondary sources with a wavelength λ when the primary and secondary sources are in the same plane, whereas it is found to be 0.55λN1/2 for the 3D case. The size of the quiet zones generated by PALs is similar to that observed with traditional omnidirectional loudspeakers; however, the effects of using PALs on the sound field outside the target zone is much smaller due to their sharp radiation directivity and slow decay rate along the propagation distance. Experimental results are also presented to validate these numerical simulations.

An experimental study on transfer function estimation using acoustic modelling and singular value decomposition.

Transfer functions relating sound source strengths and the sound pressure at field points are important for sound field control. Recently, two modal domain methods for transfer function estimation have been compared using numerical simulations. One is the spatial harmonic decomposition (SHD) method, which models a sound field with a series of cylindrical waves; while the other is the singular value decomposition (SVD) method, which uses prior sound source location information to build an acoustic model and obtain basis functions for sound field modelling. In this paper, the feasibility of the SVD method using limited measurements to estimate transfer functions over densely spaced field samples within a target region is demonstrated experimentally. Experimental results with various microphone placements and system configurations are reported to demonstrate the geometric flexibility of the SVD method compared to the SHD method. It is shown that the SVD method can estimate broadband transfer functions up to 3099 Hz for a target region with a radius of 0.083 m using three microphones, and allow flexibility in system geometry. Furthermore, an application example of acoustic contrast control is presented, showing that the proposed method is a promising approach to facilitating broadband sound zone control with limited microphones.

Regulating role of abscisic acid on cadmium enrichment in ramie (Boehmeria nivea L.).

Abscisic acid (ABA) is known as an important hormone regulating plant stress resistance, such as salt, drought and heavy metal resistance. However, the relationship between ABA and cadmium (Cd) enrichment in ramie (Boehmeria nivea L.) is still unclear to date. This study aimed to reveal the effect of ABA on Cd enrichment in ramie, and we received the following results: (1) Under Cd treatment, the Cd uptake of ramie increased with the increase of Cd concentration, but the chlorophyll content decreased. Under Cd treatment, the ABA content was highest in roots of ramie, followed by that in old leaves, and lowest in new leaves. Long-time treatment of high Cd concentration reduced the ability of endogenous ABA biosynthesis. (2) Spraying ABA on ramie plants (SORP) and adding ABA directly to the culture solution (ADCS) with low concentration can promote the growth of ramie and increase the amount of Cd uptake, and the effect of SORP is better. (3) The molecular reason for the decrease of chlorophyll content due to Cd stress, may be resulted from the down-regulated expression of the chlorophyll synthesis genes (BnPAO and BnNYC1) and the up-regulated expression of the chlorophyll degradation genes (BnCHLH, BnCHLG, BnHAP3A and BnPPR1). The elevated ABA content in ramie plants may due to the up-regulated expression of the ABA synthesis related genes (BnABA1, BnNCED3, and BnNCED5) and the genes (BnABCG40, BnNFXL2, BnPYL9, BnGCR2, BnGTG1, BnBGLU1, BnUTG1, BnVHAG1 and BnABI5) that encoding ABA transport and response proteins, which was consistent with the enhance the Cd uptake in ramie. Our study revealed the relationship between ABA and Cd uptake in ramie, which provided a reference for improving the enrichment of Cd in ramie.

Using a Retro-Reflective Membrane and Laser Doppler Vibrometer for Real-Time Remote Acoustic Sensing and Control.

Microphones have been extensively studied for many decades and their related theories are well-established. However, the physical presence of the sensor itself limits its practicality in many sound field control applications. Laser Doppler vibrometers (LDVs) are commonly used for the remote measurement of surface vibration that are related to the sound field without the introduction of any such physical intervention. This paper investigates the performance and challenges of using a piece of retro-reflective film directly as an acoustic membrane pick-up with an LDV to sense its vibration to form a remote acoustic sensing apparatus. Due to the special properties of the retro-reflective material, the LDV beam can be projected to the target over a wide range of incident angles. Thus, the location of the LDV relative to the pick-up is not severely restricted. This is favourable in many acoustic sensing and control applications. Theoretical analysis and systematic experiments were conducted on the membrane to characterise its performance. One design has been selected for sensing sound pressure level above 20 dB and within the 200 Hz to 4 kHz frequency range. Two example applications-remote speech signal sensing/recording and an active noise control headrest-are presented to demonstrate the benefits of such a remote acoustic sensing apparatus with the retro-reflective material. Particularly, a significant 22.4 dB noise reduction ranging from 300 Hz to 6 kHz has been achieved using the demonstrated active control system. These results demonstrate the potential for such a solution with several key advantages in many applications over traditional microphones, primarily due to its minimal invasiveness.

Comparative transcriptome analysis reveals key genes associated with pigmentation in radish (Raphanus sativus L.) skin and flesh.

Radish (Raphanus sativus) is an important vegetable worldwide that exhibits different flesh and skin colors. The anthocyanins responsible for the red and purple coloring in radishes possess nutritional value and pharmaceutical potential. To explore the structural and regulatory networks related to anthocyanin biosynthesis and identify key genes, we performed comparative transcriptome analyses of the skin and flesh of six colored radish accessions. The transcript profiles showed that each accession had a species-specific transcript profile. For radish pigmentation accumulation, the expression levels of anthocyanin biosynthetic genes (RsTT4, RsC4H, RsTT7, RsCCOAMT, RsDFR, and RsLDOX) were significantly upregulated in the red- and purple-colored accessions, but were downregulated or absent in the white and black accessions. The correlation test, combined with metabolome (PCC > 0.95), revealed five structural genes (RsTT4, RsDFR, RsCCOAMT, RsF3H, and RsBG8L) and three transcription factors (RsTT8-1, RsTT8-2, and RsPAR1) to be significantly correlated with flavonoids in the skin of the taproot. Four structural genes (RsBG8L, RsDFR, RsCCOAMT, and RsLDOX) and nine transcription factors (RsTT8-1, RsTT8-2, RsMYB24L, RsbHLH57, RsPAR2L, RsbHLH113L, RsOGR3L, RsMYB24, and RsMYB34L) were found to be significantly correlated with metabolites in the flesh of the taproot. This study provides a foundation for future studies on the gene functions and genetic diversity of radish pigmentation and should aid in the cultivation of new valuable radish varieties.

Insertion loss of regular finite cylinder arrays with porous layers between the rows.

It has been demonstrated recently via simulations in a duct that the sound transmission loss of porous materials can be increased with embedded periodic scatterers [Jena and Qiu, J. Acoust. Soc. Am. 147, 978-983 (2020)]. In this paper, the insertion loss (IL) of two types of finite size structures constructed by installing two parallel porous sheets within rows of periodic scatterers is investigated in free field. One structure uses rigid cylindrical shells, while the other uses split ring shells, which are obtained by making uniform vertical slits in cylindrical shells. The simulation results show that the mixed structure has larger IL than the summation of the ILs of the individual periodic scatterers and two parallel porous sheets in the frequency range after the first bandgap due to Bragg diffraction, and an additional peak in IL is introduced for the split ring scatterers due to their local resonance. The amplitude, frequency, and bandwidth of the resonance peak can be tuned by changing the geometry of the slit for some specific broadband sound absorption. Finally, the experiment results obtained in an anechoic chamber are presented to support the simulation results and analyses.

The near field, Westervelt far field, and inverse-law far field of the audio sound generated by parametric array loudspeakers.

The near and far fields of traditional loudspeakers are differentiated by whether the sound pressure amplitude is inversely proportional to the propagating distance. However, the audio sound field generated by a parametric array loudspeaker (PAL) is more complicated, and in this article it is proposed to be divided into three regions: near field, Westervelt far field, and inverse-law far field. In the near field, the audio sound experiences strong local effects and an efficient quasilinear solution is presented. In the Westervelt far field, local effects are negligible so that the Westervelt equation is used, and in the inverse-law far field, a simpler solution is adopted. It is found that the boundary between the near and Westervelt far fields for audio sound lies at approximately a2/λ - λ/4, where a is transducer radius and λ is ultrasonic wavelength. At large transducer radii and high ultrasonic frequencies, the boundary moves close to the PAL and can be estimated by a closed-form formula. The inverse-law holds for audio sound in the inverse-law far field and is more than 10 meters away from the PAL in most cases. With the proposed classification, it is convenient to apply appropriate prediction models to different regions.

Broadband noise insulation of windows using coiled-up silencers consisting of coupled tubes.

It has been demonstrated that a staggered window achieves better noise reduction performance than a traditional single glazing one at middle to high frequencies while maintaining a degree of natural ventilation. There is, however, little improvement in the low frequency range. In contrast, this work proposes to apply coiled-up silencers consisting of coupled tubes on the side walls of staggered windows to obtain noise attenuation in a broad band, especially in the low frequency range. Each element in the silencer consists of two coupled tubes with different cross sections so that noise at more frequencies can be attenuated than that with a uniform cross section. The simulation results show that 8.8 dB overall insertion loss can be obtained between 100 and 500 Hz after applying a combination of silencers designed at 7 different frequencies, and the insertion loss of the staggered window is increased from 6.7 to 15.6 dBA between 100 and 2000 Hz for normal incident traffic noise with the proposed silencers installed. The design is validated by the experiments with a 1:4 scale down model.