Transcriptional and metabolic response against hydroxyethane-(1,1-bisphosphonic acid) on bacterial denitrification by a halophilic Pannonibacter sp. strain DN.

Biological denitrification is an environmentally sound pathway for the elimination of nitrogen pollution in wastewater treatment. Extreme environmental conditions, such as the co-existence of toxic organic pollutants, can affect biological denitrification. However, the potential underlying mechanism remains largely unexplored. Herein, the effect of a model pollutant, hydroxyethane-(1,1-bisphosphonic acid) (HEDP), a widely applied and consumed bisphosphonate, on microbial denitrification was investigated by exploring the metabolic and transcriptional responses of an isolated denitrifier, Pannonibacter sp. strain DN. Results showed that nitrate removal efficiency decreased from 85% to 50% with an increase in HEDP concentration from 0 to 3.5 mM, leading to nitrite accumulation of 204 mg L-1 in 3.5 mM HEDP. This result was due to the lower bacterial population count and reduction in the live cell percentage. Further investigation revealed that HEDP caused a decrease in membrane potential from 0.080 ±â€¯0.005 to 0.020 ±â€¯0.002 with the increase in HEDP from 0 to 3.5 mM. This hindered electron transfer, which is required for nitrate transformation into nitrogen gas. Moreover, transcriptional profiling indicated that HEDP enhanced the genes involved in ROS (O2-) scavenging, thus protecting cells against oxidative stress damage. However, the suppression of genes responsible for the production of NADH/FADH2 in tricarboxylic acid cycle (TCA), NADH catalyzation (NADH dehydrogenase) in (electron transport chain) ETC system and denitrifying genes, especially nor and nir, in response to 2.5 mM HEDP were identified as the key factor inhibiting transfer of electron from TCA cycle to denitrifying enzymes through ETC system.

Electro-photocatalytic degradation of acid orange II using a novel TiO2/ACF photoanode.

A novel photoanode was prepared by immobilizing TiO(2) film onto activated carbon fibers (TiO(2)/ACF) using liquid phase deposition (LPD) to study the electro-photocatalytic (EPC) degradation of organic compounds exemplified by an azo-dye, namely, Acid Orange II (AOII). Results demonstrated that by applying a 0.5 V bias (vs. SCE) across the TiO(2)/ACF electrode, the AOII degradation rate was increased significantly compared to that of photocatalytic (PC) oxidation. The application of an electric field promotes the separation of photogenerated electrons and holes as confirmed by electrochemical impedance spectroscopy (EIS) measurements. The structural and surface morphology of the TiO(2)/ACF electrode was characterized by field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD). SEM images showed that TiO(2) was deposited on almost every carbon fiber with an average thickness of about 200 nm with the inner space between neighboring fibers being maintained unfilled. The morphological features of the photo-anode facilitated the passage of solution as well as UV light through the felt-form electrode and created a three-dimensional environment favorable to EPC oxidation. Both the large outer surface area of the 3D electrode and the good organic adsorption capacity of the ACF support promoted high contact efficiency between AOII and TiO(2) surface. Anatase was the major crystalline TiO(2) deposited. UV-vis spectrophotometry, TOC (total organic carbon) analysis, and HPLC technique were used to monitor the concentration change of AOII and intermediates as to gain insight into the EPC degradation of AOII using the TiO(2)/ACF electrode.