The effect of intergenerational rearing on young children's self-control: The intervention effect of sports games.

Objective: To investigate the effect of different rearing arrangements on children's self-control and verify the promoting effect sports games might have on children's self-control in intergenerational rearing. Methods: A total of 72 intergenerational rearing children and 66 non-intergenerational rearing children were included in Experiment 1, in which the delay-of-gratification task was used to examine the differences in self-control among children with different rearing arrangements. In experiment 2, 70 intergenerational rearing children were included as subjects, and sports games were used to improve their self-control. Results: The results of experiment 1 showed that compared with non-intergenerational rearing children, the self-control ability of intergenerational rearing children was poor. In experiment 2, it was found that after the intervention with sports games, the self-control ability of the children in the intergenerational rearing group was effectively improved. Conclusion: Although intergenerational rearing arrangements have a certain negative impact on the self-control ability of children, the sports game intervention can be used to effectively develop the self-control ability of intergenerational rearing children.

The Influence of Mixed Teaching Mode on the Learning Effects of Psychology Students' Experiment Course in Higher Vocational Colleges.

In this paper, we are going to analyze the influence of mixed teaching mode on the teaching effect of psychology students' experiment courses in higher vocational colleges. To realize and verify these claims, 80 psychology students of grade 2020 in our school were selected for observation. Class 1 of the psychology major in our school was randomly selected as the observation group (n = 40) and Class 2 as the control group (n = 40). The mixed teaching mode and the traditional teaching mode were adopted, respectively. After intervention, the experimental examination results, experimental report score, general evaluation results of the experiment, and experimental pass rate of the observation group were higher than those before intervention (P < 0.05). The control group had no significant change before and after intervention (P > 0.05). The experimental examination results, experimental report score, general evaluation results of the experiment, and experimental pass rate of the observation group were all higher than those of the control group, with statistical significance (P < 0.05). The results show that in the observation group, 92.50% of the students believed that the mixed teaching mode could arouse the enthusiasm for learning and improve the activity of the experimental class. 97.50% of the students thought that the mixed teaching mode could stimulate students' interest in learning and improve the ability of experimental operation. 100.00% of the students thought the mixed teaching mode could be helpful in understanding the experimental. The evaluation result of the teaching mode was significantly higher than that of the control group, and the comparison result was statistically significant (P < 0.05). After receiving the mixed teaching mode, the observation group had a higher satisfaction with the teaching effect, with a satisfaction rate of 95.00%. The control group had low satisfaction with the teaching effect, with a satisfaction rate of 52.50%, and the difference was statistically significant (P < 0.05). Through the application of the mixed teaching mode combining online and offline, psychology students in higher vocational colleges can achieve better learning results and improve students' evaluation and satisfaction of teaching effect of the mixed teaching mode of psychology experiment teaching mode in higher vocational colleges.

Metabolic rate and substrate oxidation of young males with obesity at the different sleep stages.

OBJECTIVE: Insufficient and poor-quality sleep among young adults is closely related to obesity and may impact metabolic processes. The mobilization and use of endogenous substrates during sleep, especially fat oxidation, is essential for energy metabolism. This study investigated whether there are differences in sleep structure, metabolic rate, substrate oxidation, and the respiratory quotient (RQ) between young males with normal weight and obesity according to sleep stages and overnight sleep. METHODS: Fifteen young males with normal weight and fifteen with obesity posited electrodes of polysomnography (PSG) and slept in the metabolic chamber for estimation of sleep structure, sleep metabolic rate (SMR), carbohydrate oxidation (CHOO), fat oxidation (FATO), and RQ. Fat-free mass (FFM) was measured by bioelectrical impedance analysis. RESULTS: The sleep period time (p = 0.038) and total sleep time (p = 0.032) were significantly shorter in the obesity group than in the normal-weight group. The obesity group also had a longer sleep latency (p = 0.034) and more sleep-turning events (p = 0.018). CHOO/FFM and the RQ were higher in the obesity group while FATO/FFM was significantly higher in the normal-weight group. FATO/FFM was also higher in the normal-weight group in each sleep stage whereas the RQ was higher in the obesity group (p < 0.05). CONCLUSIONS: Young males with obesity showed lower fat oxidation and more dominant carbohydrate-derived fuel oxidation than normal-weight during sleep and experienced shorter sleep periods and total sleep time.

Abnormal expression of FAK and paxillin correlates with oral cancer invasion and metastasis.

Globally, the tenth most common cancer is the oral squamous cell carcinoma (OSCC) and the treatment strategy for improving of OSCC patients survival rate still remains a challenging one. Aberrant regulation of cell to extracellular matrix protein interactions leads to progression of human cancers. The focal adhesion kinase (FAK) and its downstream target paxillin have been implicated in cancer growth, migration, invasion and metastasis of different cancers. However, the clinical significance of FAK and paxillin in OSCC is not well characterized so far. In the present work, we showed that relative mRNA and protein expressions of FAK and paxillin are significantly higher in side population (SP) cells of OSCC cell line SCC-55. Concomitantly, the matrix metalloproteinase-11 (MMP-11) level is also significantly elevated in SP cells. The enhanced expression of paxillin is strongly correlated with increased chemoresistance, proliferation rate, migration and invasion potential of SP cells. In addition, inhibition of paxillin expression by RNAi makes SP cells more sensitive to chemotherapy drugs. Therefore, our results suggest that paxillin over expression might play a significant role in cancer progression, invasion and chemoresistance of OSCC.

Resting Energy Expenditure, Fetal Biometric Parameters by Ultrasound, and Birthweight in Chinese Pregnant Women With Gestational Diabetes Mellitus.

OBJECTIVES: To explore the relationships between resting energy expenditure (REE) according to indirect calorimetry, fetal biometric parameters by ultrasound, and birthweight in gestational diabetes mellitus (GDM). METHODS: Sixty-five women with GDM and 60 in the control group were enrolled. The REE, birthweight, and fetal biometric parameters according to ultrasound, including biparietal diameter, head circumference, abdominal circumference (AC), and femur length, were measured. RESULTS: The AC at 29 to 32 weeks and 37 to 40 weeks was larger in the GDM than in the control group (P < 0.01), birthweight was higher in the GDM than in the control group (P < 0.01), and women in the GDM group had higher REE than those in the control group at all stages of pregnancy (P < 0.01). In the control group, all fetal biometric parameters were correlated with birthweight at 37 to 40 weeks (r = 0.418, 0.678, 0.741, and 0.635 for biparietal diameter, head circumference, AC, and femur length, respectively, P < 0.05); however, in the GDM group, only AC was correlated with birthweight at 37 to 40 weeks (r = 0.707; P < 0.05). In the GDM group, REE was correlated with birthweight at all three stages of pregnancy (r = 0.369, 0.381, and 0.446 for 21 to 24, 29 to 32, and 37 to 40 weeks, respectively, P < 0.05), and REE was correlated with AC at 37 to 40 weeks (r = 0.431; P < 0.05). CONCLUSIONS: REE is correlated with birthweight in women with GDM from the middle to the end of pregnancy. REE by indirect calorimetry might be potential index for medical nutrition therapy in GDM.

Treadmill exercise ameliorates chronic REM sleep deprivation-induced anxiety-like behavior and cognitive impairment in C57BL/6J mice.

Various sleep disorders have deleterious effects on mental and cognitive performance. Exercise, as an alternative therapeutic strategy, exerts beneficial impacts on human health. In the present study, we aimed to evaluate the effects of 4 weeks treadmill exercise (4W-TE) on anxiety-like behavior and cognitive performance in mice exposed to 2 months REM sleep deprivation (2M-SD) (20 h per day). Behavioral performance of mice in elevated plus maze test (EPM), open field test (OFT), Y maze test (YM) and Morris water maze test (MWM) was recorded and analyzed 28 h after the last day of sleep deprivation. After behavioral tests, various neurotransmitters including norepinephrine (NE), dopamine (DA), serotonin (5-HT) and γ-aminobutyric acid (GABA) in mouse hippocampus were quantified using high performance liquid chromatography. The hippocampal levels of insulin-like growth factor-1 (IGF-1) and brain derived neurotrophic factor (BDNF) were further detected using ELISA. Behavioral data indicated that 2M-SD exposure induced anxiety-like behaviors and cognitive impairment, as evidenced by the decreased open-arm entries in EPM, reduced central area travels in OFT, declined spontaneous alteration in YM and prolonged escaping latency in MWM. In addition, 2M-SD exposure increased NE and DA, decreased 5-HT and GABA, and reduced IGF-1 and BDNF levels in mouse hippocampus. Interestingly, all these behavioral, neurochemical and neurobiological changes can be ameliorated by 4W-TE training. In summary, these findings confirm the beneficial impacts of exercise on health and provide further experimental evidence for future application of exercise as an alternative therapy against the mental and cognitive problems in patients with sleep disorders.

Diagnosis of Compressed Nerve Root in Lumbar Disc Herniation Patients by Surface Electromyography.

OBJECTIVE: To establish a logistic regression model using surface electromyography (SEMG) parameters for diagnosing the compressed nerve root at L5 or S1 level in patients with lumbar disc herniation (LDH). METHODS: This study recruited 24 patients with L5 nerve root compression and 23 patients with S1 nerve root compression caused by LDH from May 2014 to May 2016. SEMG signals from the bilateral tibialis anterior and lateral gastrocnemius were measured. The root mean square (RMS), the RMS peak time, the mean power frequency (MPF), and the median frequency (MF) were analyzed. The accuracy, sensitivity, and specificity values were calculated separately. The areas under the curve (AUC) of the receiver-operating characteristic (ROC) curve and the kappa value were used to evaluate the accuracy of the SEMG diagnostic model. RESULTS: The accuracy of the SEMG model ranged from 85.71% to 100%, with an average of 93.57%. The sensitivity, specificity, AUC, and kappa value of the logistic regression model were 0.98 ± 0.05, 0.92 ± 0.09, 0.95 ± 0.04 (P = 0.006), and 0.87 ± 0.11, respectively (P = 0.001). The final diagnostic model was: P=1-11+ey; y = 10.76 - (5.95 × TA_RMS Ratio) - (0.38 × TA_RMS Peak Time Ratio) - (5.44 × 44 × LG_RMS Peak Time Ratio). L5 nerve root compression is diagnosed when P < 0.5 and S1 nerve root compression when P ≥ 0.5. CONCLUSIONS: The logistic regression model developed in this study showed high diagnostic accuracy in detecting the compressed nerve root (L5 and S1 ) in these patients with LDH.

Algorithm to improve accuracy of energy expended in a room calorimeter.

The whole-room indirect calorimeter is considered as important equipment for human energy expenditure measurement, but noise reduction in the system remains a challenge. A selective filtering method (SFM) was designed to improve the accuracy of the computation of O2 consumption rate ([Formula: see text]) and CO2 production rate ([Formula: see text]), based on two facts: (1) the rapid changes of [Formula: see text], [Formula: see text] and respiratory quotient (RQ) in human should be accompanied by physical activity; (2) the oxygen consumption and the carbon dioxide production should not be negative because living humans do not generate oxygen, nor consume carbon dioxide. The performance of SFM was compared with the moving average method, the central difference method and the wavelet de-noising method. The range of [Formula: see text] and [Formula: see text] in the empty room (the background noise) is reduced from -130.00-146.00 ml/min to -26.00-24.00 ml/min, and from -20.50-12.50 ml/min to -3.99-4.19 ml/min, by SFM. The background noise was added to simulated rectangular and sinusoidal signals that were used to evaluate the four methods over different time periods (64, 32, 16 and 8 min). The highest signal-to-noise ratio and the lowest deviation were achieved by SFM. Abnormal metabolic rates and RQs were corrected and compensated with measurement accuracy of 98.51 ± 0.3 % for 24-h alcohol burning tests. The results of the study showed that SFM can significantly improve [Formula: see text] and [Formula: see text] measurements.

Energetic Assessment of the Nonexercise Activities under Free-Living Conditions.

Nonexercise activities (NAs) are common types of physical activity in daily life and critical component in energy expenditure. However, energetic assessment of NA, particularly in free-living subjects, is a technical challenge. In this study, mechanical modeling and portable device were used to evaluate five common types of NA in daily life: sit to stand, lie to sit, bowing while standing, squat, and right leg over left. A human indirect calorimeter was used to measure the activity energy expenditure of NA. Mechanical work and mechanical efficiency of NA were calculated for mechanical modeling. Thirty-two male subjects were recruited for the study (20 subjects for the development of models and 12 subjects for evaluation of models). The average (mean ± SD) mechanical work of 5 NAs was 2.31 ± 0.50, 2.88 ± 0.57, 1.75 ± 0.55, 3.96 ± 1.25, and 1.25 ± 0.51 J/kg·m, respectively. The mean mechanical efficiencies of those activities were 22.0 ± 3.3%, 26.5 ± 5.1%, 19.8 ± 3.7%, 24.0 ± 5.5%, and 26.3 ± 5.5%. The activity energy expenditure estimated by the models was not significantly different from the measurements by the calorimeter (p > 0.05) with accuracies of 102.2 ± 20.7%, 103.7 ± 25.8%, 105.6 ± 14.6%, 101.1 ± 28.0%, and 95.8 ± 20.7%, respectively, for those activities. These findings suggest that the mechanical models combined with a portable device can provide an alternative method for the energetic analysis of nonexercise activities under free-living condition.

[Mechanism of chloroquine in promoting sensitivity of chemotherapeutics in oral squamous cell carcinoma CAL-27 cell line to cisplatin].

PURPOSE: To study the role and mechanism of autophagy in chemotherapy of oral squamous cell carcinoma, and provide theoretical evidence to improve chemotherapeutic efficacy of oral squamous cell carcinoma patients. METHODS: The cell survival rate changes induced by cisplatin (DDP) and chloroquine (CQ) in CAL-27 cells were assayed by methyl thiazolyl tetrazolium method(MTT). The LC3-II expression level was detected by laser scanning confocal microscope; The apoptotic rate was determined by flow cytometry. SPSS17.0 software package was used for statistical analysis. RESULTS: MTT results showed that compared with the control group, the cell survival rate reduced with the increasing time of DDP and CQ treatment; The optimal concentration of CAL-27 cells was 5 mg/L after treatment with CQ. IC50 of the CAL-27 cells was 5 mg/L after treatment with DDP; MTT results showed that the cell survival rate of CQ+DDP group was significantly lower than control group, CQ group and DDP group (P<0.05). With the action of CQ and DDP to CAL-27 cells for 48 hours, immunofluorescence results showed that the average fluorescence intensity of DDP group was significantly higher than the other 3 groups (P<0.05), while it was significantly lower in CQ group than the other 3 groups (P<0.05). With the action of CQ and DDP to CAL-27 cells for 48 hours, flow cytometry results showed that the cell apoptosis rate of DDP group and CQ+DDP group were significantly higher than control group and CQ group. The cell apoptosis rate of CQ+DDP group was significantly higher than DDP group (P<0.05). With the action of CQ and DDP to CAL-27 cells for 48 hours, cells in G1 phase of DDP group and CQ+DDP group increased, indicating G1 phase blockage. The cell count in G1 phase of CQ+DDP group was significantly higher than DDP group (P<0.05). CONCLUSIONS: Inhibition of autophagy can enhance the chemotherapeutic sensitivity of DDP in CAL-27 cells. Autophagy in CAL-27 cells is an important mechanism for chemotherapy resistance of oral squamous cell carcinoma. Autophagy inhibitor may have significant potential to be a novel chemotherapeutic sensitizer for oral squamous cell carcinoma.

[Study of exercise heart rate variability based on correlation dimension].

This paper is mainly devoted to the relationship between the correlation dimension (CD) of exercise heart rate variability (EHRV) and the status of cardiovascular function. Hypertensive patients and healthy people were enrolled in two contrast groups. Dynamic electrocardiograph (ECG) in step exercise was recorded. EHRV was extracted by wavelet transform. The CDs in the whole course of exercise and in three stages of exercise were calculated, the three stages being named rest-before-exercise, during-exercise, and after-exercise. SPSS software was used for statistical analysis. The results revealed all the CDs showed significant difference between two groups except that in the stage of during exercise. By discrimination analysis, the average correct rate was 92.2%. It indicated that the CD in stress status probably could be an effective non-linear parameter for assessing cardiovascular function status.

[Value of three-dimensional contrast-enhanced magnetic resonance angiography in the diagnosis of mesenteric arteriosclerosis].

OBJECTIVE: To assess the clinical value of three-dimensional contrast-enhanced magnetic resonance angiography (3D-CE-MRA) in the diagnosis of mesenteric arteriosclerosis. METHODS: 3D-CE-MRA of the mesenteric arteries was performed in 21 patients with 23 healthy subjects as the control. After 3D image reconstruction and maxi intense projection, and the abnormalities of the mesenteric arteries were observed and analyzed. The diameter and number of the arterial branches were compared between the patients and the control subjects. RESULTS: All the 21 patients suffered arteriosclerosis in the arteries other than the mesenteric arteries. On 3D-CE-MRA, mesenteric arteriosclerosis was characterized by thinning of the arterial trunk, luminal stenosis, irregular arterial margins and homo- or heterogeneous thickening of vascular walls. Multiple filling defects were found in the mesenteric artery lumens with reduced second order branches, which showed rigid lining with dashed line appearance or disappeared in some cases. The inferior mesenteric arteries were seen in only 2 patients. The diameters of superior and inferior mesenteric arteries were 3.8-/+0.32 mm and 1.20-/+0.12 mm in the patients, significantly smaller than those of in the control subjects (6.51-/+1.01 mm and 2.90-/+0.90 mm, respectively, P<0.01). The number of the mesenteric artery branch of the patients was also significantly reduced as compared with that in the control subjects (P<0.05). In som cases, the intestinal enhancement was attenuated with the intestinal contraction, dilatation and lowering of the intestinal tension. CONCLUSION: 3D-CE-MRA can clearly display mesenteric arteriosclerosis and secondary intestinal changes, and provides a useful means for the diagnosis and assisting the therapy of mesenteric arteriosclerosis.