RESUMEN
In recent years, many strategies have been developed for the biological synthesis of different types of metal nanoparticles, which have been successfully synthesized from various plant extracts and analyzed. Recent studies have demonstrated that nanoparticles have highly promising antimicrobial, antiviral, and anti-cancer properties. In the present study, biological synthesis of Ricinuscommunis leaves was performed with iron and silver nanoparticles. The synthesized iron and silver nanoparticles were characterized by UV-Vis spectroscopy, Fourier transform infrared (FT-IR), X-Ray Diffraction (XRD), Scanning electron microscopy (SEM) with Energy dispersive spectroscopy (EDS), and Transmission electron microscopy (TEM). GC-MS analysis of the Ricinus communis revealed the secondary metabolites of total phenolic and flavonoid contents of the extract, which are responsible for the bio-reduction reaction during nanoparticle synthesis. The UV-Vis spectrum shows Plasmon peaks at 340 nm and 440 nm for iron and silver nanoparticles, respectively. XRD results revealed crystalline structure, while TEM, SEM, and EDS identified iron and silver with mostly cuboidal and spherical shapes. Antimicrobial activity was also performed, and it was found that both nanoparticles were active against Salmonella typhi (6 ± 0.073) and (7 ± 0.040), Staphylococcus aureus, and Aspergillus flavus. MIC was also performed, and AgNPs gave a better bactericidal effect against Staphylococcus aureus.
RESUMEN
Aspergillus oryzae, isolated from the starch rich litter soil, produced amylase when banana fruit stalk was used as substrate in a solid state fermentation system. The effects of incubation period, pH, temperature and various carbon sources on the production of amylase were studied. A maximum yield of 380U/mg was recorded on 96th hour of incubation. The amylase is tolerant to wide range of initial culture pH values (3 to 8) and temperature (25 to 35 degrees C), with an optimum pH of 5 and temperature of 35 degrees C. In SSF addition of starch (1%) increased the amylase production, when compared with other carbon sources used.
