Celiac Disease: A Review from Genetic to Treatment.

Celiac disease (CD) is a complex disorder influenced by genetic and environmental factors. When people with a genetic predisposition to CD consume gluten, an inflammatory response is triggered in the small intestine, and this reaction can be alleviated by the elimination of gluten from the diet. The clinical manifestations of CD vary greatly from person to person and begin at a young age or in adulthood. Influence of genetic factors on CD development is evident in carriers of the DQ2 and/or DQ8 allele. HLA genotypes are associated with gut colonization by bacteria, particularly in individuals suffering from CD. In addition, beneficial gut microbes are crucial for the production of DPP-4, which plays a key role in immune function, as well as metabolic and intestinal health. Therefore, probiotics have been recommended as a complementary food supplement in CD.

Genotypic and Phenotypic-Based Assessment of Antibiotic Resistance and Profile of Staphylococcal Cassette Chromosome mec in the Methicillin-Resistant Staphylococcus aureus Recovered from Raw Milk.

BACKGROUND: Multidrug resistant methicillin-resistant Staphylococcus aureus (MRSA) bacteria are determined to be one of the chief causes of foodborne diseases around the world. PURPOSE: This research was done to assess the genotypic and phenotypic profiles of antibiotic resistance and distribution of Staphylococcus cassette chromosome mec (SCCmec) types amongst the MRSA bacteria recovered from raw milk. METHODS: Five-hundred and ninety raw milk samples were collected and examined. MRSA bacteria were recognized using susceptibility evaluation toward oxacillin and cefoxitin disks. Profile of antibiotic resistance genes and SCCmec types were determined using the PCR. Antibiotic resistance pattern of isolates was examined using the disk diffusion. RESULTS: Thirty-nine out of 590 raw milk samples (6.61%) were positive for S. aureus. Twenty-eight out of 39 (71.79%) bacteria were defined as MRSA bacteria. Raw buffalo (80%) milk samples had the maximum incidence of MRSA, while raw camel (33.33%) had the minimum. MRSA bacteria harbored the maximum incidence of resistance toward penicillin (100%), tetracycline (100%), erythromycin (82.14%), gentamicin (78.57%) and trimethoprim-sulfamethoxazole (78.57%). Incidence of resistance toward more than eight classes of antibiotic agents was 28.57%. The most frequently distinguished antibiotic resistance markers were blaZ (100%), tetK (85.71%), dfrA1 (71.42%), aacA-D (67.85%), ermA (50%) and gyrA (42.85%). SCCmec IVa (29.62%), V (25%), III (14.81%) and IVb (11.11%) were the most frequently distinguished types. CONCLUSION: Raw milk of dairy animals maybe sources of multidrug resistant MRSA which pose a hygienic threat concerning the consumption of raw milk in Iran. Nevertheless, further investigations are necessary to understand supplementary epidemiological features of MRSA in raw milk.

Genetic association study of promoter variation rs3761549 in the FOXP3 gene of Iranian patients diagnosed with brain tumour.

Forkhead box P3 (FOXP3) gene (Gene ID: 50943, Xp11.23) is an X-linked gene that encodes FOXP3 protein, an essential transcription factor in CD4+ CD25+ FOXP3 regulatory T (Treg) cells. FOXP3 mutation has been linked with the pathogenesis of several tumours; however, little is known about the role of single-nucleotide polymorphism (SNP) in its promoter region and its correlation with brain tumour. In the present study, we have investigated the association between SNPs in the promoter region of FOXP3 gene, a promoter SNP, -2383 C/T (rs3761549) with susceptibility to brain cancer in a population of Iran. The distribution of case, control, age and sex was balanced and with rs3761549 C/T allele frequencies distribution also falling in Hardy-Weinberg equilibrium (P = 0.053 and 0.062). The allele C of rs3761549 is lower in the brain tumour cases when compared with the controls (364 vs 392, P = 0.005). The frequency of combined T variant genotype (TT + CT) was significantly higher in the brain cancer cases compared with the controls (28 vs 8, P = 0.001), which was consistent with the T allele distribution. When we used the CC genotype as a reference, we found that both CT and TT genotypes were associated with a higher risk of developing brain tumour (odds ratio [OR], 0.3583; 95% confidence interval [CI], 0.164-0.8197 and OR, 0; 95% CI, 0-0.4118, respectively).