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Carcinoma Basocelular , Dermoscopía , Microscopía Confocal , Neoplasias Cutáneas , Humanos , Carcinoma Basocelular/diagnóstico por imagen , Carcinoma Basocelular/cirugía , Carcinoma Basocelular/patología , Dermoscopía/métodos , Neoplasias Cutáneas/diagnóstico por imagen , Neoplasias Cutáneas/patología , Neoplasias Cutáneas/cirugía , Microscopía Confocal/métodos , Ultrasonografía/métodos , Masculino , Femenino , Anciano , Persona de Mediana EdadRESUMEN
Lymphatic malformation is a benign lesion, seldom affecting the gingiva. Gingival lesions are characterized by pebbly hyperplasia, occasional pain, and bleeding. The treatment for large and exceptional areas of involvement may face difficulties. Herein we report a rare case of gingival lymphatic malformation in a 10-year-old girl.
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Encía , Cabeza , Niño , Femenino , Encía/patología , Cabeza/patología , Humanos , Hiperplasia/patologíaAsunto(s)
Dermatitis Atópica , Eccema , Animales , Dermoscopía , Eccema/diagnóstico , Humanos , Lactante , InsectosRESUMEN
BACKGROUND: In China, the prevalence of superficial fungal infections of the foot is high and recurrence is common. However, a prospective, large-scale and multicentre study on the aetiology of superficial fungal infections of the foot is still lacking. OBJECTIVES: To study the epidemiology of aetiological agents of superficial fungal infections of the foot in urban outpatients in mainland China, as well as to understand the aetiology features of the pathogenic agent. METHODS: The study was designed as a multicentre, prospective epidemiological survey. A total of 1704 subjects were enrolled from seven geographical areas in mainland China. For each subject, one mycological sample and one bacterial sample were collected. KOH wet mount examination and culture were performed at local laboratories. The bacterial results were only reported in those with positive mycology. Further morphological identification and, if necessary, molecular biological identification were conducted in a central laboratory. RESULTS: Of 1704 enrolled subjects, 1327 (77.9%) subjects had positive fungal culture results. The incidence of dermatophytes, yeasts and moulds was 90.1%, 8.1% and 1.1%, respectively. The most frequently isolated aetiological agent (fungus) was Trichophyton rubrum. Moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections. The most frequently isolated bacterial genus in patients was Staphylococcus. CONCLUSION: This study prospectively investigated the clinical and mycological features of human dermatophytosis in mainland China. T rubrum was the most frequently isolated fungus, and moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections.
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Dermatomicosis , Pie/microbiología , Adulto , Arthrodermataceae/aislamiento & purificación , Arthrodermataceae/patogenicidad , China/epidemiología , Dermatomicosis/epidemiología , Dermatomicosis/etiología , Dermatomicosis/patología , Femenino , Pie/patología , Hongos/aislamiento & purificación , Hongos/patogenicidad , Humanos , Incidencia , Masculino , Micosis/epidemiología , Micosis/etiología , Micosis/patología , Pacientes Ambulatorios , Prevalencia , Estudios Prospectivos , Levaduras/aislamiento & purificación , Levaduras/patogenicidadRESUMEN
The 2019 novel coronavirus infection has brought a great challenge in prevention and control of the national epidemic of coronavirus disease 2019 (COVID-19) in China. During the fight against the epidemic of COVID-19, properly carrying out pre-examination and triage for patients with skin lesions and fever has been a practical problem encountered in hospitals for skin diseases as well as clinics of dermatology in general hospitals. Considering that certain skin diseases may have symptom of fever, and some of the carriers of 2019 novel coronavirus and patients with COVID-19 at their early stage may do not present any symptoms of COVID-19, to properly deal with the visitors to clinics of dermatology, the Chinese Society of Dermatology organized experts to formulate the principles and procedures for pre-examination and triage of visitors to clinics of dermatology during the epidemic of COVID-19.
RESUMEN
We describe a 28-year-old man with linear atrophoderma of Moulin (LAM), whose serum immunological markers were abnormal (including antinuclear antibody, ribonucleoprotein, immunoglobulin M and anti-SM antibody). In addition, however, a histological analysis identified unexpected connective tissue disease changes in this patient. We speculate that the pathogenesis of LAM is associated with immunity or that LAM itself is a kind of connective tissue disease.
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Hiperpigmentación/inmunología , Piel/patología , Adulto , Anticuerpos Antinucleares/sangre , Atrofia/inmunología , Atrofia/patología , Enfermedades del Tejido Conjuntivo/patología , Humanos , Hiperpigmentación/sangre , Hiperpigmentación/patología , Inmunoglobulina M/sangre , Masculino , Proteínas Nucleares snRNP/inmunologíaRESUMEN
Abstract Tinea faciei is a relatively uncommon dermatophyte infection entailing atypical clinical symptoms, usually misdiagnosed and treated with corticosteroids. The authors describe a case of tinea faciei on the right eyebrow caused by Trichophyton interdigitale. The patient was an 18-year-old girl, who had an inflammatory plaque with a scaly, pustular surface on the right eyebrow and upper eyelid, which had persisted for over 1 month. She was once misdiagnosed as having eczema and was treated using corticosteroid cream. A diagnosis of tinea faciei was made based on direct microscopy and culture. The sequencing of the nuclear ribosomal ITS region and β-tubulin gene of the isolate established its T. interdigitale lineage. The patient was cured by treatment with systemic terbinafine in combination with topical application of 1% naftifine-0.25% ketaconazole cream for 2 weeks.
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Humanos , Femenino , Adolescente , Tiña/patología , Trichophyton/aislamiento & purificación , Cejas/microbiología , Cejas/patología , Dermatosis Facial/microbiología , Dermatosis Facial/patología , Tiña/tratamiento farmacológico , Ureasa/análisis , Microscopía Electrónica de Rastreo , Resultado del Tratamiento , Dermoscopía , Dermatosis Facial/tratamiento farmacológico , Antifúngicos/uso terapéutico , Naftalenos/uso terapéuticoRESUMEN
BACKGROUND: Itraconazole has been used to treat fungal infections, in particular invasive fungal infections in infants or neonates in many countries. DATA SOURCES: Literature search was conducted through Ovid EMBASE, PubMed, ISI Web of Science, CNKI and Google scholarship using the following key words: "pediatric" or "infant" or "neonate" and "fungal infection" in combination with "itraconazole". Based on the literature and our clinical experience, we outline the administration of itraconazole in infants in order to develop evidence-based pharmacotherapy. RESULTS: Of 45 articles on the use of itraconazole in infancy, 13 are related to superficial fungal infections including tinea capitis, sporotrichosis, mucosal fungal infections and opportunistic infections. The other 32 articles are related to systemic fungal infections including candidiasis, aspergillosis, histoplasmosis, zygomycosis, trichosporonosis and opportunistic infections as caused by Myceliophthora thermophila. CONCLUSION: Itraconazole is safe and effective at a dose of 5 mg/kg per day in a short duration of therapy for superficial fungal infections and 10 mg/kg per day for systemic fungal infections in infants. With a good compliance, it is cost-effective in treating infantile fungal infections. The profiles of adverse events induced by itraconazole in infants are similar to those in adults and children.
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Antifúngicos/uso terapéutico , Fungemia/tratamiento farmacológico , Fungemia/microbiología , Itraconazol/uso terapéutico , Micosis/tratamiento farmacológico , Micosis/microbiología , Adolescente , Factores de Edad , Niño , Preescolar , China , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Medicina Basada en la Evidencia , Femenino , Fungemia/fisiopatología , Humanos , Lactante , Masculino , Micosis/fisiopatología , Seguridad del Paciente/estadística & datos numéricos , Índice de Severidad de la Enfermedad , Factores Sexuales , Resultado del TratamientoAsunto(s)
Dermoscopía/métodos , Lupus Eritematoso Discoide/diagnóstico , Adulto , Humanos , MasculinoRESUMEN
Tinea faciei is a relatively uncommon dermatophyte infection entailing atypical clinical symptoms, usually misdiagnosed and treated with corticosteroids. The authors describe a case of tinea faciei on the right eyebrow caused by Trichophyton interdigitale. The patient was an 18-year-old girl, who had an inflammatory plaque with a scaly, pustular surface on the right eyebrow and upper eyelid, which had persisted for over 1 month. She was once misdiagnosed as having eczema and was treated using corticosteroid cream. A diagnosis of tinea faciei was made based on direct microscopy and culture. The sequencing of the nuclear ribosomal ITS region and ß-tubulin gene of the isolate established its T. interdigitale lineage. The patient was cured by treatment with systemic terbinafine in combination with topical application of 1% naftifine-0.25% ketaconazole cream for 2 weeks.
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Cejas/microbiología , Cejas/patología , Dermatosis Facial/microbiología , Dermatosis Facial/patología , Tiña/patología , Trichophyton/aislamiento & purificación , Adolescente , Antifúngicos/uso terapéutico , Dermoscopía , Dermatosis Facial/tratamiento farmacológico , Femenino , Humanos , Microscopía Electrónica de Rastreo , Naftalenos/uso terapéutico , Terbinafina , Tiña/tratamiento farmacológico , Resultado del Tratamiento , Ureasa/análisisAsunto(s)
Dermatosis del Pie/tratamiento farmacológico , Morfolinas/uso terapéutico , Onicomicosis/tratamiento farmacológico , Administración Tópica , Estudios de Seguimiento , Dermatosis del Pie/diagnóstico , Humanos , Lactante , Laca , Masculino , Onicomicosis/diagnóstico , Índice de Severidad de la Enfermedad , Resultado del TratamientoRESUMEN
BACKGROUND: Extranodal natural killer/T-cell (NK/T cell) lymphoma, nasal-type, is a rare lymphoma. Skin is the second most common site of involvement after the nasal cavity/nasalpharynx. The aim of this study was to investigate the clinicopathologic features, immunophenotype, T cell receptor (TCR) gene rearrangement, the association with Epstein-Barr virus (EBV) infection and p53 gene mutations of the lymphoma. METHODS: The clinicopathologic analysis, immunohistochemistry, in situ hybridization for EBER1/2, TCR gene rearrangement by polymerase chain reaction (PCR), mutations of p53 gene analyzed by PCR and sequence analysis were employed in this study. RESULTS: In the 19 cases, the tumor primarily involved the dermis and subcutaneous layer. Immunohistochemical staining showed that most of the cases expressed CD45RO, CD56, CD3ε, TIA-1 and GrB. Three cases were positive for CD3 and two cases were positive for CD30. Monoclonal TCRγ gene rearrangement was found in 7 of 18 cases. The positive rate of EBER1/2 was 100%. No p53 gene mutation was detected on the exon 4 - 9 in the 18 cases. Fifteen cases showed Pro (proline)/Arg (arginine) single nucleotide polymorphisms (SNPs) on the exon 4 at codon 72. The expression of p53 protein was 72% (13/18) immunohistochemically. CONCLUSIONS: Cutaneous NK/T-cell lymphoma is a rare but highly aggressive lymphoma with poor prognosis. No p53 gene mutation was detected on the exon 4 - 9, and Pro/Arg SNPs on p53 codon 72 were detected in the cutaneous NK/T-cell lymphoma. The overexpression of p53 protein may not be the result of p53 gene mutation.
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Infecciones por Virus de Epstein-Barr/diagnóstico , Linfoma de Células T/diagnóstico , Receptores de Antígenos de Linfocitos T/metabolismo , Neoplasias Cutáneas/diagnóstico , Proteína p53 Supresora de Tumor/genética , Adolescente , Adulto , Anciano , Niño , Infecciones por Virus de Epstein-Barr/genética , Infecciones por Virus de Epstein-Barr/metabolismo , Femenino , Humanos , Inmunohistoquímica , Inmunofenotipificación , Hibridación in Situ , Linfoma de Células T/genética , Linfoma de Células T/metabolismo , Masculino , Persona de Mediana Edad , Mutación , Receptores de Antígenos de Linfocitos T/genética , Neoplasias Cutáneas/genética , Neoplasias Cutáneas/metabolismo , Adulto JovenRESUMEN
OBJECTIVE: To establish a new method using cremophor EL test for the identification of Malassezia. METHODS: The suspension of Malassezia fur fur was incubated in a broth containing different concentrations of cremophor EL at 32 degrees C. Cell growth of the yeast was counted by hematocytometer at the 4th, 6th, 8th, 10th and 12th day, respectively. The optimum concentration of cremophor EL for Malassezia was determined by means of variance analysis. Then the 11 species of Malassezia were inoculate in the agar containing the optimum concentration of cremophor EL, and the growth of colony of each species were observed and compared with those inoculated under traditional conditions. RESULTS: The optimum concentration of cremophor EL for Malassezia fur fur was 4% (P < 0.05). The growth of the 11 species in the agar containing 4% cremophor EL were identical with those inoculated under the traditional conditions. The new method was easy to perform and demanded less culture medium and strains of yeast. CONCLUSION: The cremophor EL test can improve the efficiency and reduce the cost of identification of Malassezia species.
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Glicerol/análogos & derivados , Malassezia/aislamiento & purificación , Medios de Cultivo , Glicerol/farmacología , Malassezia/clasificación , Malassezia/crecimiento & desarrolloRESUMEN
OBJECTIVE: To amplify the nucleotide sequences of desmoglein 4 (Dsg4) extracellular domains (EC1-4) from human skin tissue, and then to investigate their roles in pemphigus vulgans (PV) pathogenesis. METHODS: RNA was obtained from normal human skin tissue and then cDNA was synthesized by RT-PCR. The target gene fragments of desmoglein 4 extracellular domains (EC1-4) were amplified by PCR. With the technique of gene recombination, these target gene fragments were inserted into pET32a plasmids respectively by T4 DNA ligase, which formed the recombinant plasmids used to transform the E. coli DH5alpha competent germs. Screening of transformant germs was done by LB medium with Ampicillin. The DNA sequences of positive recombinants were then identified. The epitopes of four recombinant proteins of Dsg4 in PV patients were analyzed by ELISA. RESULTS: Four DNA bands with all the length of 350 bp were obtained by RT-PCR. Consequently four expression plasmids of desmoglein 4 extracellular domains were constructed, of which the nucleotide sequences and open reading frames were proved to be correct. It showed that the recombinant proteins of Dsg4 domains EC1, EC2, EC3 and EC4 reacted to PV patients' sera, but not to normal sera. CONCLUSION: The above data indicate that the epitopes of Dsg4 may play a role in the pathogenesis of PV.
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Desmogleínas/genética , Desmogleínas/inmunología , Pénfigo/genética , Estudios de Casos y Controles , Clonación Molecular , Desmogleínas/química , Desmogleínas/aislamiento & purificación , Electroforesis en Gel de Poliacrilamida , Epítopos/inmunología , Escherichia coli/genética , Espacio Extracelular/genética , Humanos , Pénfigo/patología , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Proteínas Recombinantes/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
OBJECTIVE: To investigate the effect of ultraviolet B (UVB) on Interleukin-8 secretion in human keratinocyte cell line. METHODS: The concentration of IL-8 was detected by ELISA kit 24 h after human keratinocytes (HKC) were irradiated by different doses of UVB, and the level of IL-8 was also determined at different times after the same dose of UVB irradiation. RESULTS: The secretion of IL-8 was increased after the HKC were irradiated by UVB, the effect was dose-dependent when UVB ranged from 10 to 40 mJ/cm2, and there was statistically significant difference between the IL-8 level of UVB groups (20-70 mJ/cm2) and the control (0 mJ/cm2) (P<0.01). The level of IL-8 was increased 1 h after the irradiation of 30 mJ/cm2 UVB, and it reached the peak at 12 h. There was statistically significant difference between the IL-8 levels detected at different times (3 h, 6 h, 12 h, 24 h after irradiation) and that at 0 h (P<0.01). CONCLUSION: UVB increase the secretion of IL-8, and the effect is dose-dependent to some extent.
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Interleucina-8/metabolismo , Queratinocitos/metabolismo , Rayos Ultravioleta , Línea Celular , Relación Dosis-Respuesta en la Radiación , Humanos , Interleucina-8/efectos de la radiación , Queratinocitos/efectos de la radiación , Piel/citologíaRESUMEN
OBJECTIVE: To purify and identify recombinant Varicella-Zoster Virus Glycoprotein E. METHODS: The recombinant plasmid pGEX-VZVgE was induced by isopropyl-beta-D-thiogalactoside (IPTG), the fusion protein was purified with affinity chromatography column; then the purified fusion protein was cleaved by thrombin, and the product's antigenicity was examined by Western blot. RESULTS: The product of pGEX-VZVgE induced by IPTG was separated from the mixture proteins by the affinity chromatography column, the expressed fusion protein's relative molecular mass was about 98 x 10(3). After cleavage, the obtained VZV Glycoprotein E's relative molecular mass was about 72 x 10(3); the purified fusion protein and VZV Glycoprotein E were single band by SDS-PAGE. The available antigenicity of Glycoprotein E was confirmed by Western blot. CONCLUSION: Purification of VZV Glycoprotein E with affinity chromatography is an effective method. It provides a foreground for studies on the application of VZV gE.