Critical evaluation of electroactive wetlands: traditional and modern advances.

In the field of sustainable wastewater management, electroactive wetlands (EW), or constructed wetland-microbial fuel cells (CW-MFC), are an emerging technology. With the growing problem of untreated wastewater, the emphasis must shift to decentralisation of wastewater treatment infrastructure, and CW-MFC can be an excellent choice. This review provides a chronologically organized account of the design and configuration of CW-MFCs developed between 2010 and 2023. The research on CW-MFC has mainly focused on material, positioning and number of electrodes; use of electroconductive media and filler materials; flow regime; algal-based CW-MFC and multistage setups. Compared to traditional constructed wetlands (CW) and microbial fuel cells (MFC), CW-MFCs have a number of advantages, including better treatment efficiency, faster organic matter utilisation, lower capital and land requirements and a smaller carbon footprint. However, there are some limitations as well, such as upscaling and viable electricity generation, which are covered in more detail in the article. Moreover, the economics of this technology is also evaluated. The microbiology of a CW-MFC and its influence on its performance are also elaborated. Recent advancements in this field in terms of design, configuration and performance are discussed. Finally, the knowledge gaps that must be addressed before this technique can be successfully implemented on a large scale are highlighted, along with specific recommendations. This article aims to advocate for EWs as an ideal decentralised wastewater treatment technique, while also shedding light on the areas that still need to be worked on.

Interaction of physico-chemical parameters with Shannon-Weaver Diversity Index based on phytoplankton diversity in coastal water of Diu, India.

Phytoplankton acts as carbon sinks due to photosynthetic efficacy and their diversity is expressed by SWDI (Shannon-Weaver Diversity Index), which depends on water quality parameters. The coastal water of Diu was studied for three seasons, and the relationship between different parameters and SWDI was established. Subsequently, an attempt was made to build up a prediction model of SWDI based on multilayer perceptron Artificial neural network (ANN) using the R programme. Analysis shows interrelationship between the water quality parameters and phytoplankton diversity is same in linear principal component analysis (PCA) and neural network model. Variations of different parameters depend on seasonal changes. The ANN model shows that ammonia and phosphate are key parameters that influence the SWDI of phytoplankton. Seasonal variation in SWDI is related to variation in water quality parameters, as explained by both ANN and PCA. Hence, the ANN model can be an important tool for coastal environmental interaction study.

Pre-to-post Diwali air quality assessment and particulate matter characterization of a western coastal place in India.

Diwali has become an occasion of air and noise pollution, and the release of particulate matter and toxic gases has chronic and acute effects on people and their environment. Thus, an air quality assessment study was done by CSIR-CSMCRI covering the pre-to-post Diwali 2021 period (5 days) in the three locations (traffic, residential, and control) of Bhavnagar. The average 24-h concentration of PM10 (380 µg/m3), PM2.5 (182.2 µg/m3), and SPM (403 µg/m3) was significantly higher during Diwali, exceeding the National Ambient Air Quality Standards (NAAQS). The concentrations of SO2 and NO2 were 121.8 µg/m3 and 102.1 µg/m3. Metals like Zn, Al, Pb, and Mn were found in higher concentrations during the study. The air quality index (AQI) was maximum on Diwali, resembling very poor air quality. More elements and oxides were detected in PM2.5 (S, Al, Mg, Ba, and Zn and their oxides) than in PM10 (Fe and S) through WDXRF. Water-soluble anions like SO42-, Cl-, and NO3- were observed during the study, with a higher SO42- (64%) on Diwali. The PM10 morphology and mapping of elements were done using SEM-EDX. Emerging contaminants, specifically phthalate groups, were detected through GCMS. The enrichment factor (EF) showed Zn and Pb originating from anthropogenic activities. The air quality data was validated using a variance test, least significance difference (LSD), correlation, and principal component analysis (PCA). This paper is the first to highlight the air quality assessment during Diwali for a western coastal place in India. It is time to implement regulations on burning firecrackers for pollution reduction, aiming to achieve a sustainable atmosphere.

Remediation and characterization of emerging and environmental pollutants from residential wastewater using a nature-based system.

The nature-based systems (NBS) are nature inspired, unflagging, efficient, and budget friendly ideas that evolved as ideal technologies for wastewater treatment. The present study deals with the purification of residential wastewater through the NBS, covering three seasons. The NBS embedded with the Canna lily effectively eliminated organic matter, nutrients, and heavy metals. Nearly 57.2-75.2% COD, 69.9-83.2% BOD, 73.4-90.6% TSS, 51.1-71.6% PO43--P, 66.3-84.8% NH4+-N, 52-61.5% NO3--N, and 68-70.6% NO2--N removal were achieved. Heavy metals like Al, Cr, Mn, Fe, Ni, Cu, Zn, Mo, and Pb were removed, with a 98.25% reduction in the total bacterial count. The pollutant removal's kinetics was calculated using first-order kinetics. The mass removal rate of BOD was high in monsoon (22.3 g/m2/d), and COD was high in summer (36.4 g/m2/d). Organic compound removal (65.2%), including emerging pollutants, was observed by gas chromatography-mass spectrometry (GCMS) analysis of water and Canna samples. Wavelength dispersive X-ray fluorescence spectrometer (WDXRF) studied the elements and oxides retention by media and accumulation by the plant. The CHN content of the Canna and its morphological study was checked using the carbon CHNS analyzer and scanning electron microscope-energy dispersive X-ray (SEM-EDX), respectively. The performance of the NBS was validated using variance, correlation, and principal component analysis (PCA). This study shows the NBS effects on the remediation of environmental and emerging contaminants from residential wastewater and further use it for horticultural activities, thereby achieving sustainable development goals.

Monitoring and assessment of Dracaena-based constructed vertical flow wetlands treating textile dye wastewater.

The monitoring and assessment of multiple constructed vertical flow wetlands (CVFWs) treating textile dye wastewater (metanil yellow as dye) are studied covering three seasons. Three CVFWs (CVFW-1, dye-5 mg/l; CVFW-2, dye-50 mg/l; and CVFW-3, dye-100 mg/l) and a control (dye-5 mg/l) were used. The CVFWs with Dracaena (an ornamental plant) efficiently removed contaminants like dye, COD, NH4+-N, and PO43--P from the wastewater under varying inlet dye concentrations, indicating its dependence on meteorological conditions. Substantial dye removal was observed to be maximum in summer (control, 44.3%; CVFW-1, 75.1%; CVFW-2, 76.1%; CVFW-3, 46%), but lesser in winter (control, 45%; CVFW-1, 73.1%; CVFW-2, 76.8%; CVFW-3, 42.6%) and minimum in monsoon (control, 40.8%; CVFW-1, 63.5%; CVFW-2, 51.6%; CVFW-3, 37.1%), respectively. Efficiency was less in CVFW-3 as it observed plant stress due to higher inlet dye concentration. COD removal was higher in winter, followed by summer and monsoon. A first-order kinetic model was used to investigate the efficiency of the CVFW system w.r.t. contaminant removal. Various functional groups were characterized using Fourier transform infrared spectroscopy (FTIR) from the inlet and outlet water samples of different CVFWs. The Dracaena accumulated various elements and oxides during the treatment with no stress on its health. No effects on plant health highlight the suitability of Dracaena for textile wastewater treatment. The results were validated using statistical tools like the Mann-Whitney U test and principal component analysis (PCA).

Pre-to-post COVID-19 lockdown and their environmental impacts on Ghoghla beach and Somnath beach, India.

Environmental impact of COVID-19 imposed lockdown (2020) and the new normal condition (2021) on two different beaches of India (Ghoghla beach, Diu and Somnath beach, Veraval) were compared with the pre-lockdown era, 2013. The lockdown phase favored the natural restoration of the beaches and showed infinitesimal values of the parameters tested when compared with the pre-lockdown regime. However, the new normal situation in 2021 opened up the accessibility of these beaches to the tourists and pilgrims resulting in significant changes of water quality. The release of diluted sewage mixed with freshwater from the Somnath town to the sea has led to the drastic change in beach water quality. The mean cadmium concentration increased drastically in beach waters (Ghoghla: 1.35, 0.28 and 7.09 µg/L; Somnath: 0.45, 0.28 and 0.58 µg/L) during pre-to-post lockdown, respectively. However, post-lockdown resulted in the rise of toxic heavy metals in the sediments of Somnath beach but Ghoghla beach remained to be pristine which may be due to the Blue Flagship status. The total number of marine bacteria was higher during 2013 and 2021 when compared during lockdown describing greater human interventions. For instance, Vibrio spp. count in Ghoghla beach water during pre-lockdown phase was 7733 CFU/mL and this value reduced to 70 and 5 CFU/mL in the lockdown and post-lockdown phases. Interestingly, the diversity of planktonic and benthic components showed a different trend from pre-to-post lockdown due to significant change in the inorganic nutrients and metal bioaccumulation. To our knowledge, this will be the first comprehensive assessment to report the environmental and ecological health of Ghoghla beach and Somnath beach during the pre-to-post lockdown.

Impact of 2nd wave of COVID-19-related lockdown on coastal water quality at Diu, western coast of India and role of total alkalinity on bacterial loads.

A detailed coastal water monitoring near Diu coast, western part of India was performed from October, 2020 to May, 2021 covering the 2nd lockdown time. Average monthly fluctuation from 7 different sampling stations of total 9 physico-chemical parameters such as pH, salinity, turbidity, nitrite (NO2), nitrate (NO3), ammonia (NH3), phosphate (PO4), total alkalinity and silicate were recorded. Initially, Mann-Kendall trend test for all the 9 parameters showed non-zero trend, which may be either linear or non-linear. During 2nd lockdown period, there was a fluctuation of value for parameters like pH, salinity, nitrate, nitrite and phosphate. Average total bacterial count and differential bacterial count also gradually decreased from March, 2021 sampling. Principal component analysis (PCA) plot covering all the physico-chemical parameters as well as the differential bacterial count showed a distinct cluster of all bacterial count with total alkalinity value. Subsequently, mathematical equation was formulated between total alkalinity value and all differential bacterial count. Upto our knowledge, this is the first report where mathematical equation was formulated to obtain value of different bacterial load based on the derived total alkalinity value of the coastal water samples near Diu, India.

In-depth performance study of an innovative decentralized multistage constructed wetland system treating real institutional wastewater.

The performance of an innovative decentralized multistage constructed wetland (DMCW) treating institutional wastewater is studied covering three seasons. The DMCW system with Canna lily efficiently removed organics contaminants like COD and BOD, and nutrients from the wastewater, showing its dependency on meteorological factors. Overall the performance is maximum in summer and least in monsoon, with a COD removal of 85.6% in summer followed by 82.5% in winter and 61.2% in monsoon. Removal of TSS (67.7-85.5%), PO43--P (52.1-64.4%), NH4+-N (56.6-71.6%), NO3--N (47.3-63.4%) and NO2--N (62-75.4%) were achieved along with heavy metals like Cr, Mn, Fe, Ni, Cu, Zn, As, Cd, Hg and Pb. Removal of pathogens like Vibrio is >98%, E. coli 95%, Pseudomonas 99%, and Aeromonas 63% was observed. Mass removal rate of COD was maximum in summer (97.3 g/m2/d) followed by winter (78.7 g/m2/d) and monsoon (43.5 g/m2/d). Majority of organics removal during the treatment was highlighted through Gas Chromatography-Mass Spectrometry (GCMS) and Fourier Transform Infrared Spectroscopy (FTIR) confirmed wastewater to be complex. The Canna lily accumulated various elements and oxides during the treatment with no stress on its health. The treated water quality is within the permissible limits and stands suitable for irrigational purposes. Better plant health and increased microbial diversity in the garden proves the suitability of treated water for irrigational activities. The results were validated using statistical tools like Mann-Whitney U test and principal component analysis.

New normal baseline data during nationwide lock down due to Covid 19 pandemic in the world's largest ship recycling yard at Alang, India.

A detailed study to generate the new normal baseline data has been carried out during nationwide lockdown (May 12 to May 16, 2020) covering sampling for ambient air, coastal water, coastal sediments, fish and bioaccumulation of heavy metals, in an around Alang, the world's biggest ship recycling yard. The lockdown data were compared with 2018 and 2019 observed data. PM10 values during lockdown were reduced by 3.75 to 4.5 times as compared with previous 2 years. Similarly, four-fold reduction of PM2.5 and SPM values was observed during lockdown. The gaseous pollutants like NO2 and O3 are within safe limit. Overall air quality index (AQI) improved significantly during lockdown. Similarly, there was drastic reduction in the majority of the nutrient parameters in the coastal water. Different heavy metal concentration in the coastal sediments samples also showed strong reduction during lockdown sampling in comparison with other two sampling. This proves that the coastal environment has its efficient self-cleaning potentials if there is considerable reduction in the anthropogenic as well as industrial activities. Diversity of phytoplankton and zooplankton also increased. The results were validated using statistical techniques like analysis of variance and least significance difference (LSD).

Kinetics of carbon and nitrogen assimilation by heterotrophic microorganisms during wastewater treatment.

The present study highlights microbial assimilation of carbon (glucose) and nitrogen (NH3-N) from wastewater using heterotrophic bioconversion process. Experiments were conducted to study the role of heterotrophic microbes towards removal of carbon and nitrogen at varying initial concentrations of carbon (COD, 533 to 1600 mg/l) and nitrogen (NH3-N, 73 to 249 mg/l) keeping the initial biomass of microorganisms constant. Removal of COD and ammonia from wastewater represented a first-order rate reaction, upon analysis of kinetics, indicating that the rate of reaction is dependent on the initial concentration of nutrients available. Rate equations were developed using the Monod model, and the maximum specific consumption rate (k4) and half saturation constant (Ks) values for NH3-N and COD were found to be 2.59 mg/l/h and 64.13 mg/l/h and 38.46 mg/l and 2162.69 mg/l, respectively. Assimilation of NH3-N followed the Freundlich isotherm model. The mass transfer coefficient for COD and NH3-N were found to be 0.13 h-1 and 0.81 h-1 respectively. The NH3-N is converted to N2O during nitrification, and observed values of N2O coincided with the empirically predicted values indicating the activity of heterotrophic nitrifiers. The regeneration/doubling time of heterotrophic microbial biomass varied from 26 to 121 h. Statistical techniques, viz. analysis of variance, multi-linear regression analysis and principal component analysis, validated the results.

Removal of phosphorous and nitrogen from wastewater in Brachiaria-based constructed wetland.

Considering the prevalence of eutrophication of water bodies, sustainable treatment technologies like constructed wetlands (CWs) have come up as a promising alternate for nutrient removal and wastewater treatment. The present study was undertaken to investigate the potential of Brachiaria-based constructed wetland for removal of phosphorus and nitrogen in different seasons of a sub-tropical region. The CW cell could efficiently remove phosphate and nitrogen under varying influent concentrations across different seasons. Average removal of total phosphate increased from 55.2% (winter) to 78.5% (spring), 80.7% (autumn), and 85.6% (summer), and maximum removal rate was 384.4 mg/m2-day during the summer season. The soluble/available phosphate was removed on priority owing to its easy bio-availability. The removal efficiency of Brachiaria increased with increasing influent phosphate concentration (5-20 mg/l), if supplemented with nitrogen maintaining the N:P ratio of 5:1. This highlighted the characteristic of Brachiaria to absorb chemical shocks w.r.t. phosphate. The neutral pH (6.2-8.3) and oxidising conditions in rhizosphere ruled out possibility of binding of phosphate with cations (Ca, Fe, and Al) in sediments. Ambient temperature and sunshine hours regulated evapotranspiration and hence nutrient removal. Simultaneous removal of nitrogen (75.6-84.6%) by Brachiaria indicated that it can serve dual purpose of nutrient removal and fodder-production for livestock, thus serving as a sustainable prototype for rural communities in sub-tropical regions.

Role of microorganisms in emission of nitrous oxide and methane in pulse cultivated soil under laboratory incubation condition.

Soil from a pulse cultivated farmers land of Odisha, India, have been subjected to incubation studies for 40 consecutive days, to establish the impact of various nitrogenous fertilizers and water filled pore space (WFPS) on green house gas emission (N2O & CH4). C2H2 inhibition technique was followed to have a comprehensive understanding about the individual contribution of nitrifiers and denitrifiers towards the emission of N2O. Nevertheless, low concentration of C2H2 (5 ml: flow rate 0.1 kg/cm(2)) is hypothesized to partially impede the metabolic pathways of denitrifying bacterial population, thus reducing the overall N2O emission rate. Different soil parameters of the experimental soil such as moisture, total organic carbon, ammonium content and nitrate-nitrogen contents were measured at regular intervals. Application of external N-sources under different WFPS conditions revealed the diverse role played by the indigenous soil microorganism towards green house gas emission. Isolation of heterotrophic microorganisms (Pseudomonas) from the soil samples, further supported the fact that denitrification might be prevailing during specific conditions thus contributing to N2O emission. Statistical analysis showed that WFPS was the most influential parameter affecting N2O formation in soil in absence of an inhibitor like C2H2.