Frequent detection of Saffold cardiovirus in adenoids.

Saffold virus (SAFV) is classified into the Cardiovirus genus of the Picornaviridae family. Up to now, eleven genotypes have been identified however, their clinical significance remains unclear. Here, we investigated the presence of SAFV in asymptomatic patients admitted for adenoidectomy. A total of 70 adenoid tissue samples were collected from children with clinical symptoms caused by hypertrophy of adenoids but without symptoms of airway infection. Samples were investigated for SAFV by RT-nested PCR and sequence analysis. Eleven of 70 (15.7%) samples were positive for SAFV. Nasopharyngeal swabs were available from 45 children just before surgery. SAFV was rarely found and only in children with SAFV-positive adenoids 2/8. Our findings indicate that the presence of SAFV seems to be more frequent in adenoid tissue than expected. This could support the notion of a longer than previously anticipated persistence of SAFV nucleic acids in the respiratory tract and possibly a chronic infection. Further investigations are necessary to establish the role of SAFV infection in humans.

Evaluation of cellulose pads as a method to detect cytomegalovirus DNA in neonatal urine.

Background Several approaches exist to screen neonates for congenital cytomegalovirus infection. We here describe a new method using cellulose pads for urine collection and its evaluation in an experimental and a clinical setting. Methods We systematically tested the effect of storage duration of the pads after exposure to cytomegalovirus-positive urine, meconium contamination and specimen handling on the cytomegalovirus load and the detection rate. Further, the method was tested in clinical practice in a cohort of 500 neonates. Results Following exposure of urine pads with cytomegalovirus-positive urine, the viral load decreased after 15 min, 12 h, 24 h, and 7 days to 63.2%, 42.1%, 31.6%, and 9.3% of the baseline value. Cytomegalovirus detection rate after seven days was 100%. Contamination with meconium resulted in a comparable reduction of the viral load. The detection rate for dried urine pads after seven days was 93.3%. In clinical practice, urine collection from pads was successful in 73.6% by the first attempt and in 26.4% by the second attempt. Conclusions Urine collection using cellulose pads seems feasible regardless of a reduction of the cytomegalovirus load due to exposure to the pad itself or to meconium. Drying of the exposed urine pad should be avoided.

Two cases of sepsis-like illness in infants caused by human parechovirus traced back to elder siblings with mild gastroenteritis and respiratory symptoms.

Sepsis and sepsis-like illness in neonates and infants are serious emergencies. Recently, human parechovirus type 3 (HPeV-3) has been identified as a further etiologic agent of these conditions. We report two unlinked cases of infant HPeV-3 sepsis-like illness whose sources could be traced back to elder siblings with mild gastroenteritis and respiratory symptoms.

Human parvovirus 4 in nasal and fecal specimens from children, Ghana.

Nonparenteral transmission might contribute to human parvovirus 4 (PARV4) infections in sub-Saharan Africa. PARV4 DNA was detected in 8 (0.83%) of 961 nasal samples and 5 (0.53%) of 943 fecal samples from 1,904 children in Ghana. Virus concentrations ≤ 6-7 log(10) copies/mL suggest respiratory or fecal-oral modes of PARV4 transmission.

Performance of the novel Qiagen artus QS-RGQ viral load assays compared to that of the Abbott RealTime system with genetically diversified HIV and hepatitis C Virus plasma specimens.

We compared two novel Qiagen QS-RGQ viral load assays with the established Abbott RealTime assays on a highly diversified panel of 121 human immunodeficiency virus (HIV) and 107 hepatitis C virus (HCV) specimens. The quantifications correlated well for all HIV and HCV types, but Qiagen yielded higher HCV concentrations than Abbott, predominantly in genotypes 4 and 5.

Poor clinical sensitivity of rapid antigen test for influenza A pandemic (H1N1) 2009 virus.

Influenza A pandemic (H1N1) 2009 virus RNA was detected by reverse transcription-PCR in 144 clinical samples from Bonn, Germany. A common rapid antigen-based test detected the virus in only 11.1% of these samples. The paramount feature of rapid test-positive samples was high virus concentration. Antigen-based rapid tests appear unsuitable for virologic diagnostics in the current pandemic.

Persistence of novel human parvovirus PARV4 in liver tissue of adults.

Human parvovirus 4 (PARV4) is a recently identified virus whose biology, epidemiology and pathogenic potential have yet to be determined. Recently, it was reported that PARV4 DNA persists in tissues of some HIV-infected individuals, whilst PARV4 DNA was not detected in tissues of subjects not infected with HIV. In the present study, liver tissue from 87 individuals, none of who were infected with HIV, with the exception of a single subject, was analyzed for the presence of PARV4 DNA. Overall, PARV4 DNA was detected in 13 specimens (15%). In other tissues examined, PARV4 genotype 2 (also termed PARV5) DNA was detected in one of four paired bone marrow specimens. Tissue viral loads did not exceed 100 copies per microg of genomic DNA. In addition, serum samples from 40 of these individuals all tested negative for PARV4 DNA. In the subjects analyzed in this study, PARV4 genotype 2 appeared to be genetically more heterogeneous than PARV4 genotype 1. The results show that PARV4 DNA can be detected in liver, and that infection with PARV4 is not restricted to HIV-infected individuals. Previous studies showing the presence of PARV4 in plasma, suggest that during infection with PARV4, a viraemic stage occurs, allowing systemic spread to a variety of tissues.