RESUMEN
BACKGROUND: Leptin, one of the most prominent mediators released from adipocytes, influences neuronal activity in the central nervous system. The enteric nervous system (ENS) expresses leptin receptors but consequence of activation of these receptors on enteric neuron activity has not been systematically studied. An adipocyte-ENS axis is suggested by close apposition between enteric nerves and adipocytes. The aim of this study was to investigate the effects of leptin on guinea-pig submucous and myenteric neurons. METHODS: Using voltage sensitive dye imaging, we recorded neural responses to application of leptin (0.0625 nmol L(-1)) in myenteric and submucous neurons, nicotine (10 µmol L(-1)) served as a reference for neuronal excitation. Mucosal ion secretion and muscle activity were measured in vitro with Ussing and organ bath techniques, respectively. KEY RESULTS: Leptin induced spike discharge in 13.6% of submucous neurons and in 8.2% of myenteric neurons (1.1 ± 0.9 and 1.2 ± 1.0 Hz, respectively). Although there was an overlap of nicotine and leptin responses, 38.5% of submucous and 25% of myenteric neurons activated by leptin did not respond to nicotine. Leptin did not inhibit ongoing spike discharge or fast excitatory postsynaptic potentials. Leptin (0.0625 nmol L(-1)) did not affect mucosal secretion or muscle activity suggesting a subtle modulatory action of leptin at the level of the ENS. CONCLUSIONS & INFERENCES: Leptin activates submucous and myenteric neurons indicating relevance for adipocyte-ENS signaling. These results set the basis for further studies to reveal the functional correlate of the neural action of leptin in the ENS.
Asunto(s)
Potenciales de Acción/efectos de los fármacos , Sistema Nervioso Entérico/citología , Leptina/farmacología , Plexo Mientérico/efectos de los fármacos , Neuronas/efectos de los fármacos , Plexo Submucoso/efectos de los fármacos , Potenciales de Acción/fisiología , Adipocitos/citología , Adipocitos/fisiología , Animales , Relación Dosis-Respuesta a Droga , Sistema Nervioso Entérico/fisiología , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Potenciales Postsinápticos Excitadores/fisiología , Estimulantes Ganglionares/farmacología , Cobayas , Masculino , Modelos Animales , Plexo Mientérico/citología , Plexo Mientérico/fisiología , Neuronas/citología , Neuronas/fisiología , Nicotina/farmacología , Transducción de Señal/fisiología , Plexo Submucoso/citología , Plexo Submucoso/fisiologíaAsunto(s)
4-Quinolonas , Antiinfecciosos Urinarios/administración & dosificación , Antiinfecciosos , Gonorrea/tratamiento farmacológico , Quinolinas/administración & dosificación , Quinolonas , Uretritis/tratamiento farmacológico , Adolescente , Adulto , Femenino , Gonorrea/complicaciones , Humanos , Masculino , Persona de Mediana Edad , Uretritis/complicacionesRESUMEN
Disorder by hemorrhoids can occasionally be caused by sacral herpes zoster. Only exact local inspection in connection with the anamnestic statement of neuralgic pain as well as hurting sensations of the rectum leads to the proper diagnosis.