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Current diagnostic and therapeutic approaches for gliomas have limitations hindering survival outcomes. We propose spectroscopic magnetic resonance imaging as an adjunct to standard MRI to bridge these gaps. Spectroscopic MRI is a volumetric MRI technique capable of identifying tumor infiltration based on its elevated choline (Cho) and decreased N-acetylaspartate (NAA). We present the clinical translatability of spectroscopic imaging with a Cho/NAA ≥ 5x threshold for delineating a biopsy target in a patient diagnosed with non-enhancing glioma. Then, we describe the relationship between the undertreated tumor detected with metabolite imaging and overall survival (OS) from a pilot study of newly diagnosed GBM patients treated with belinostat and chemoradiation. Each cohort (control and belinostat) were split into subgroups using the median difference between pre-radiotherapy Cho/NAA ≥ 2x and the treated T1-weighted contrast-enhanced (T1w-CE) volume. We used the Kaplan-Meier estimator to calculate median OS for each subgroup. The median OS was 14.4 months when the difference between Cho/NAA ≥ 2x and T1w-CE volumes was higher than the median compared with 34.3 months when this difference was lower than the median. The T1w-CE volumes were similar in both subgroups. We find that patients who had lower volumes of undertreated tumors detected via spectroscopy had better survival outcomes.
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Glioblastoma , Glioma , Ácidos Hidroxámicos , Sulfonamidas , Humanos , Proyectos Piloto , Análisis Espectral , Biopsia , Imagen por Resonancia Magnética , ColinaRESUMEN
Brainstem tissue microstructural properties change across the adult lifespan. However, studies elucidating the biological processes that govern brainstem maturation and degeneration in-vivo are lacking. In the present work, conducted on a large cohort of 140 cognitively unimpaired subjects spanning a wide age range of 21 to 94 years, we implemented a multi-parameter approach to characterize the sex- and age differences. In addition, we examined regional correlations between myelin water fraction (MWF), a direct measure of myelin content, and diffusion tensor imaging indices, and transverse and longitudinal relaxation rates to evaluate whether these metrics provide information complementary to MWF. We observed region-dependent differences in myelin content and axonal density with age and found that both exhibit an inverted U-shape association with age in several brainstem substructures. We emphasize that the microstructural differences captured by our distinct MRI metrics, along with their weak associations with MWF, strongly indicate the potential of using these outcome measures in a multi-parametric approach. Furthermore, our results support the gain-predicts-loss hypothesis of tissue maturation and degeneration in the brainstem. Indeed, our results indicate that myelination follows a temporally symmetric time course across the adult life span, while axons appear to degenerate significantly more rapidly than they mature.
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Tronco Encefálico/patología , Longevidad , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Tronco Encefálico/diagnóstico por imagen , Estudios de Cohortes , Imagen de Difusión Tensora , Femenino , Humanos , Modelos Lineales , Masculino , Persona de Mediana Edad , Vaina de Mielina/metabolismo , Caracteres Sexuales , Agua , Adulto JovenRESUMEN
Adequate cerebral blood flow (CBF) is essential to a healthy central nervous system (CNS). Previous work suggests that CBF differs between men and women, and declines with age and certain pathologies, but a highly controlled systematic study across a wide age range, and incorporating white matter (WM) regions, has not been undertaken. Here, we investigate age- and sex-related differences in CBF in gray matter (GM) and WM regions in a cohort (N = 80) of cognitively unimpaired individuals over a wide age range. In agreement with literature, we find that GM regions exhibited lower CBF with age. In contrast, WM regions exhibited higher CBF with age in various cerebral regions. We attribute this new finding to increased oligodendrocyte metabolism to maintain myelin homeostasis in the setting of increased myelin turnover with age. Further, consistent with prior studies, we found that CBF was higher in women than in men in all brain structures investigated. Our work provides new insights into the effects of age and sex on CBF. In addition, our results provide reference CBF values for the standard ASL protocol recommended by the ISMRM Perfusion Study Group and the European ASL in Dementia consortium. Thus, these results provide a foundation for further investigations of CNS perfusion in a variety of settings, including aging, cerebrovascular diseases, and dementias.
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Envejecimiento , Circulación Cerebrovascular/fisiología , Sustancia Gris/fisiología , Imagen por Resonancia Magnética , Sustancia Blanca/fisiología , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Femenino , Voluntarios Sanos , Humanos , Masculino , Persona de Mediana Edad , Factores SexualesRESUMEN
BACKGROUND: Myelin loss and cerebral blood flow (CBF) decline are central features of several neurodegenerative diseases. Myelin maintenance through oligodendrocyte metabolism is an energy-demanding process, so that myelin homeostasis is particularly sensitive to hypoxia, hypoperfusion or ischaemia. However, in spite of its central importance, little is known about the association between blood supply and myelin integrity. OBJECTIVE: To assess associations between cortical and subcortical CBF, and subcortical myelin content, in critical brain white matter regions. MATERIALS AND METHODS: MRI was performed on a cohort of 67 cognitively unimpaired adults. Using advanced MRI methodology, we measured whole-brain longitudinal and transverse relaxation rates (R1 and R2 ), sensitive but non-specific markers of myelin content, and myelin water fraction (MWF), a direct surrogate of myelin content, as well as regional CBF, from each of these participants. RESULTS: All quantitative relaxometry metrics were positively associated with CBF in all brain regions evaluated. These associations between MWF or R1 and CBF, and, to a lesser extent, between R2 and CBF, were statistically significant in most brain regions examined, indicating that lower regional cortical or subcortical CBF corresponds to a decrease in local subcortical myelin content. Finally, all relaxometry metrics exhibited a quadratic, inverted U-shaped, association with age; this is attributed to the development of myelination from young to middle age, followed by progressive loss of myelin in later years. CONCLUSIONS: In this first study examining the association between local blood supply and myelin integrity, we found that myelin content declines with CBF across a wide age range of cognitively normal subjects.
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Previous in-vivo magnetic resonance imaging (MRI)-based studies of age-related differences in the human brainstem have focused on volumetric morphometry. These investigations have provided pivotal insights into regional brainstem atrophy but have not addressed microstructural age differences. However, growing evidence indicates the sensitivity of quantitative MRI to microstructural tissue changes in the brain. These studies have largely focused on the cerebrum, with very few MR investigations addressing age-dependent differences in the brainstem, in spite of its central role in the regulation of vital functions. Several studies indicate early brainstem alterations in a myriad of neurodegenerative diseases and dementias. The paucity of MR-focused investigations is likely due in part to the challenges imposed by the small structural scale of the brainstem itself as well as of substructures within, requiring accurate high spatial resolution imaging studies. In this work, we applied our recently developed approach to high-resolution myelin water fraction (MWF) mapping, a proxy for myelin content, to investigate myelin differences with normal aging within the brainstem. In this cross-sectional investigation, we studied a large cohort (nâ¯=â¯125) of cognitively unimpaired participants spanning a wide age range (21-94 years) and found a decrease in myelination with age in most brainstem regions studied, with several regions exhibiting a quadratic association between myelin and age. We believe that this study is the first investigation of MWF differences with normative aging in the adult brainstem. Further, our results provide reference MWF values.
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Envejecimiento , Agua Corporal/diagnóstico por imagen , Tronco Encefálico/diagnóstico por imagen , Imagen por Resonancia Magnética , Vaina de Mielina/ultraestructura , Neuroimagen , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Estudios Transversales , Femenino , Humanos , Imagen por Resonancia Magnética/métodos , Masculino , Persona de Mediana Edad , Neuroimagen/métodos , Adulto JovenRESUMEN
Changes in longitudinal relaxation time (T1) and proton density (PD) are sensitive indicators of microstructural alterations associated with various central nervous system diseases as well as brain maturation and aging. In this work, we introduce a new approach for rapid and accurate high-resolution (HR) or ultra HR (UHR) mapping of T1 and apparent PD (APD) of the brain with correction of radiofrequency field, B1, inhomogeneities. The four-angle method (FAM) uses four spoiled-gradient recalled-echo (SPGR) images acquired at different flip angles (FA) and short repetition times (TRs). The first two SPGR images are acquired at low-spatial resolution and used to accurately map the active B1+ field with the recently introduced steady-state double angle method (SS-DAM). The estimated B1+ map is used in conjunction with the two other SPGR images, acquired at HR or UHR, to map T1 and APD. The method is evaluated with numerical, phantom, and in-vivo imaging measurements. Furthermore, we investigated imaging acceleration methods to further shorten the acquisition time. Our results indicate that FAM provides an accurate method for simultaneous HR or UHR mapping of T1 and APD in human brain in clinical high-field MRI. Derived parameter maps without B1+correction suffer from large inaccuracies, but this issue is well-corrected through use of the SS-DAM. Furthermore, the use of SPGR imaging with short TR and phased-array coil acquisition permits substantial imaging acceleration and enables robust HR or UHR T1 and APD mapping in a clinically acceptable time frame, with whole brain coverage obtained in less than 2â¯min or 5â¯min, respectively. The method exhibits high reproducibility and benefits from the use of the conventional SPGR sequence, available in all preclinical and clinical MRI machines, and very simple modeling to address a critical outstanding issue in neuroimaging.
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Mapeo Encefálico/métodos , Encéfalo/anatomía & histología , Procesamiento de Imagen Asistido por Computador/métodos , Imagen por Resonancia Magnética/métodos , Adulto , Algoritmos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Fantasmas de Imagen , Protones , Reproducibilidad de los Resultados , Tiempo , Adulto JovenRESUMEN
The relationship between regional brain myelination and aging has been the subject of intense study, with magnetic resonance imaging perhaps the most effective modality for elucidating this. However, most of these studies have used nonspecific methods to probe myelin content, including diffusion tensor imaging, magnetization transfer ratio, and relaxation times. In the present study, we used the BMC-mcDESPOT analysis, a direct and specific method for imaging of myelin water fraction (MWF), a surrogate of myelin content. We investigated age-related differences in MWF in several brain regions in a large cohort of cognitively unimpaired participants, spanning a wide age range. Our results indicate a quadratic, inverted U-shape, relationship between MWF and age in all brain regions investigated, suggesting that myelination continues until middle age followed by decreases at older ages. We also observed that these age-related differences vary across different brain regions, as expected. Our results provide evidence for nonlinear associations between age and myelin in a large sample of well-characterized adults, using a direct myelin content imaging method.
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Envejecimiento/metabolismo , Agua Corporal/metabolismo , Encéfalo/metabolismo , Imagen por Resonancia Magnética/métodos , Vaina de Mielina/metabolismo , Anciano , Anciano de 80 o más Años , Femenino , Humanos , MasculinoRESUMEN
BACKGROUND: Cerebral blood flow (CBF) is an emerging biomarker for normal aging and neurodegenerative diseases. Arterial spin labeling (ASL) perfusion MRI permits noninvasive quantification of CBF. However, high-quality mapping of CBF from ASL imaging is challenging, largely due to noise. NEW METHOD: We demonstrate the ability of the recently introduced nonlocal estimation of multispectral magnitudes (NESMA) filter to greatly improve determination of CBF estimates from ASL imaging data. We evaluated the results of NESMA-ASL for CBF mapping from data obtained on human brain (n = 10) across a wide age range (21-74 years) using a standard clinical protocol. Results were compared to those obtained from unfiltered images or filtered images using conventional and advanced filters. Quantitative analyses for different spatial image resolutions and signal-to-noise ratios, SNRs, were also conducted. RESULTS: Our results demonstrate the potential of NESMA-ASL to permit high-quality high-resolution CBF mapping. NESMA-ASL substantially reduces random variation in derived CBF estimates while preserving edges and small structures, with minimal bias and dispersion in derived CBF estimates. COMPARISON WITH EXISTING METHODS: NESMA-ASL outperforms all evaluated filters in terms of noise reduction and detail preservation. Further, unlike other filters, NESMA-ASL is straightforward to implement requiring only one user-defined parameter, which is relatively insensitive to SNR or local image structure. CONCLUSIONS: In-vivo estimation of CBF in the human brain from ASL imaging data was markedly improved through use of the NESMA-ASL filter. The use of NESMA-ASL may contribute significantly to the goal of high-quality high-resolution CBF mapping within a clinically feasible acquisition time.
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Mapeo Encefálico/métodos , Encéfalo/fisiología , Procesamiento de Imagen Asistido por Computador/métodos , Angiografía por Resonancia Magnética/métodos , Adulto , Algoritmos , Encéfalo/irrigación sanguínea , Simulación por Computador , Femenino , Humanos , Masculino , Persona de Mediana Edad , Procesamiento de Señales Asistido por Computador , Relación Señal-Ruido , Marcadores de Spin , Adulto JovenRESUMEN
PURPOSE: To extend the null signal method (NSM) for B1 mapping to 3â¯T magnetic resonance imaging (MRI). BACKGROUND: The NSM operates in the steady state regime and exploits the linearity of the spoiled gradient recalled echo (SPGR) signal around the 180° flip angle (FA). Using linear regression, B1 maps are derived from three SPGR images acquired at different FAs with a short repetition time. While the conventional NSM allows accurate mapping of B1 for moderate B1 variation, we observed that this method fails for the larger B1 variations typical of high-field MRI. METHODS: We analyzed the effect of the FA range of the acquired SPGR images on B1 determination using the NSM for 3â¯T MRI through extensive numerical and in vivo analyses. B1 maps derived from the extended angle-range NSM (EA-NSM) were calculated and compared to those derived from the conventional, more restricted angle range, NSM, and to those derived from the reference, but much more time-consuming, double angle method (DAM). Furthermore, we investigated the compatibility of EA-NSM B1 mapping and the half-scan and SENSE reconstruction methods for accelerating acquisition time. RESULTS: Our results show that the use of the conventional FA range leads to substantial inaccuracies in B1 determination. Both numerical and in vivo analyses demonstrate that expanding the FA range of the acquired SPGR images substantially improves the accuracy of B1 maps. Furthermore, B1 maps derived from EA-NSM were demonstrated to be quantitatively comparable to those derived from the lengthy DAM protocol. We also found that B1 maps derived from SPGR images using the EA-NSM and imaging acceleration methods were comparable to those derived from images acquired without acceleration. Finally, the use of half scanning combined with SENSE reconstruction permits whole-brain B1 mapping in ~1â¯min. CONCLUSIONS: The EA-NSM permits accurate, fast, and practical B1 mapping in a 3â¯T clinical setting.