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1.
Artículo en Ruso | MEDLINE | ID: mdl-15024981

RESUMEN

The immunomodulating activity of acellular pertussis vaccine (APV) and adsorbed DPT vaccine with acellular pertussis component (DPTA vaccine) was studied. The study revealed that only large doses of APV, 10 immunizing doses (ID), suppressed humoral and cell-mediated response to sheep red blood cells (SRBC). 1 ID produced no influence on the formation of antibody producing cells, but increased the development of delayed hypersensitivity (DH) to SRBC. The modulation of cell-mediated immune response, induced by APV, returned to normal after the injection of purified staphylococcal toxoid, used as immunomodulator, in doses of 0.15 BU per mouse and 1.5 BU per mouse. DPTA vaccine containing 1 ID, as well as 10 ID, produced no immunomodulating effect. This was established by the evaluation of humoral response to SRBC in CBA mice and the study of the formation of DH to SRBC in BALB/c mice. As indicated by the total of the presented data, the inclusion of APV into DPTA vaccine enhanced the immunological safety of its pertussis component.


Asunto(s)
Adyuvantes Inmunológicos/administración & dosificación , Vacunas contra Difteria, Tétanos y Tos Ferina Acelular/administración & dosificación , Inmunización , Vacunas Acelulares/administración & dosificación , Tos Ferina/prevención & control , Animales , Células Productoras de Anticuerpos/inmunología , Vacuna contra Difteria, Tétanos y Tos Ferina/administración & dosificación , Vacunas contra Difteria, Tétanos y Tos Ferina Acelular/inmunología , Relación Dosis-Respuesta Inmunológica , Evaluación Preclínica de Medicamentos , Eritrocitos/inmunología , Hipersensibilidad Tardía/etiología , Inmunidad Celular , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos CBA , Ovinos , Toxoide Estafilocócico/administración & dosificación , Vacunas Acelulares/inmunología , Tos Ferina/inmunología
2.
Artículo en Ruso | MEDLINE | ID: mdl-12886628

RESUMEN

Toxic properties of acellular pertussis vaccine (APV) and morphological changes in white mice in response to intramuscular injection of APV (without or with immunomodulator glucosaminylmuramyl dipeptide-GMDP) were under study. APV used in these experiments was developed at the Mechnikov Research Institute for Vaccines and Sera (the Russian Acad. Med. Sci.) on the basis of Bordetella pertussis cultures in synthetic fluid culture media. In experiments on acute and chronic toxicity of APV (without GMDP) increased tissue immunity reactions in spleen, thymus, liver, lungs and intestinal wall was detected. There was no difference in immunomorphological reactions in mice receiving APV with different doses of GMDP, but some difference was observed in time dynamics of tissue immunity reactions. A small dose of GMDP should be preferred (0.0001 microgram) which results in gradual growth of tissue immunity reactions less pronounced toxic reactions caused be the APV injection.


Asunto(s)
Intestinos/patología , Hígado/patología , Pulmón/patología , Vacuna contra la Tos Ferina/farmacología , Bazo/patología , Timo/patología , Adyuvantes Inmunológicos/farmacología , Animales , Inmunidad Celular , Intestinos/inmunología , Hígado/inmunología , Pulmón/inmunología , Ratones , Especificidad de Órganos , Vacuna contra la Tos Ferina/inmunología , Bazo/inmunología , Timo/inmunología , Vacunas Acelulares/inmunología , Vacunas Acelulares/farmacología
3.
Artículo en Ruso | MEDLINE | ID: mdl-11881494

RESUMEN

The humoral response of mice and rabbits to the injection of whole-cell pertussis vaccine (PV) and acellular pertussis vaccine (APV), developed at the Mechnikov Research Institute for Vaccines and Sera (Russian Acad. Med. Sci.) in Moscow, was studied. In the sera of immunized animals antibodies to the antigenic complex were determined in the direct hemagglutination (DHA) test, specific antibodies to filamentous hemagglutinin (FHA) and pertussis toxin (PT)--in the enzyme immunoassay (EIA) and antibodies neutralizing PT in a cytopathogenic dose (CPD)--in neutralization test on Chinese hamster ovary (CHO) cells. In mice and rabbits immunized with APV the antibody titers determined in the DHA test were higher than those in the animals immunized with PV. Specific antibodies titers to FHA and PT in the sera of rabbits immunized with APV were also higher than those in the sera of rabbits immunized with PV. High dilutions of sera taken from the animals immunized with APC neutralized 4-16 doses of PT in the neutralization test on CHO cells. The most important result of this study was the detection of a more pronounced immune response in the animals immunized with APV in comparison with that induced by PV according to the results obtained in EIA and in the test of PT CPD neutralization on CHO cells.


Asunto(s)
Anticuerpos Antibacterianos/biosíntesis , Bordetella pertussis/inmunología , Inmunización , Vacuna contra la Tos Ferina/inmunología , Adhesinas Bacterianas/inmunología , Animales , Anticuerpos Antibacterianos/sangre , Células CHO , Cricetinae , Pruebas de Hemaglutinación , Hemaglutininas/inmunología , Técnicas para Inmunoenzimas , Ratones , Pruebas de Neutralización , Toxina del Pertussis , Vacuna contra la Tos Ferina/administración & dosificación , Conejos , Vacunación , Vacunas Acelulares/inmunología , Vacunas de Productos Inactivados/inmunología , Factores de Virulencia de Bordetella/inmunología , Tos Ferina/prevención & control
4.
Artículo en Ruso | MEDLINE | ID: mdl-9304336

RESUMEN

The immunogenic and protective properties of acellular pertussis vaccines, prepared on the basis of B. pertussis multicomponent protective complex and the preparation containing only pertussis toxin, were studied. The study revealed that multicomponent preparations containing pertussis toxin (PT), filamentous hemagglutinin, agglutinogens, pertactin and adenylate cyclase possessed more pronounced immunobiological and protective properties in comparison with the monovalent preparation of PT, which was indicative of the expediency of developing acellular pertussis vaccines on the basis of the polyvalent protective complex as minor protective antigens seemed to enhance the protective action of pertussis toxoid, the main protective antigen.


Asunto(s)
Antígenos Bacterianos/inmunología , Bordetella pertussis/inmunología , Vacuna contra la Tos Ferina/inmunología , Toxina de Adenilato Ciclasa , Animales , Anticuerpos Antibacterianos/sangre , Antígenos Bacterianos/aislamiento & purificación , Vacuna contra Difteria, Tétanos y Tos Ferina/inmunología , Vacuna contra Difteria, Tétanos y Tos Ferina/aislamiento & purificación , Inmunización , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Toxina del Pertussis , Vacuna contra la Tos Ferina/aislamiento & purificación , Conejos , Factores de Virulencia de Bordetella/inmunología , Factores de Virulencia de Bordetella/aislamiento & purificación
5.
Artículo en Ruso | MEDLINE | ID: mdl-9304334

RESUMEN

A highly effective technology for obtaining a protective antigenic complex, including the main Bordetella pertussis protective antigens (pertussis toxin, filamentous hemagglutinin, agglutinogens, pertactin and adenylate cyclase) and isolated from the supernatant of B. pertussis cultivation medium, has been developed. A new method for the detoxication of antigenic complex which more available to preserve the protective property was suggested. Experimental batches of monovaccine and adsorbed DPT vaccine with the acellular pertussis component, possessing high protective activity and low histamine-sensitizing properties, have been obtained. The stability of protective properties both in liquid and lyophilized acellular pertussis preparations has been noted.


Asunto(s)
Antígenos Bacterianos/aislamiento & purificación , Bordetella pertussis/inmunología , Vacuna contra la Tos Ferina/aislamiento & purificación , Animales , Antígenos Bacterianos/inmunología , Bordetella pertussis/patogenicidad , Medios de Cultivo , Vacuna contra Difteria, Tétanos y Tos Ferina/inmunología , Vacuna contra Difteria, Tétanos y Tos Ferina/aislamiento & purificación , Relación Dosis-Respuesta Inmunológica , Inmunización , Inmunización Pasiva , Ratones , Vacuna contra la Tos Ferina/inmunología , Virulencia/inmunología , Tos Ferina/etiología , Tos Ferina/prevención & control
7.
Artículo en Ruso | MEDLINE | ID: mdl-7653146

RESUMEN

Some physicochemical properties of B.pertussis antigenic complexes isolated from synthetic and semisynthetic culture media have been studied. The chemical composition of these complexes has been determined. Proteins, polypeptide subunits and lipopolysaccharide forming these complexes have been characterized. The presence of two main protective substances of B.pertussis has been revealed: fimbrial hemagglutinin and B.pertussis toxin. The influence of culture medium on the level of the synthesis of B.pertussis adenylate cyclase has been studied.


Asunto(s)
Antígenos Bacterianos/análisis , Bordetella pertussis/inmunología , Antígenos Bacterianos/química , Antígenos Bacterianos/aislamiento & purificación , Bordetella pertussis/crecimiento & desarrollo , Fenómenos Químicos , Química Física , Medios de Cultivo , Electroforesis en Gel de Poliacrilamida , Concentración de Iones de Hidrógeno , Peso Molecular , Péptidos/análisis , Factores de Tiempo
9.
Artículo en Ruso | MEDLINE | ID: mdl-8184612

RESUMEN

The preclinical trial of a new cell-free pertussis vaccine has been carried out with a view to its comparison with whole-cell pertussis vaccine in respect of the toxic and protective properties of the preparations under experimental conditions. The whole-cell pertussis vaccine is characterized by pronounced toxic action causing leukocytosis and histamine-sensitization, produces a negative effect on the detoxifying function of the liver and possesses sensitizing activity. The cell-free pertussis vaccine has pronounced protective activity. In animal experiments the supposed immunization dose of this vaccine has proved to be nontoxic.


Asunto(s)
Vacuna contra la Tos Ferina/toxicidad , Animales , Relación Dosis-Respuesta Inmunológica , Evaluación Preclínica de Medicamentos , Almacenaje de Medicamentos , Inmunización , Dosificación Letal Mediana , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Vacuna contra la Tos Ferina/inmunología , Vacuna contra la Tos Ferina/aislamiento & purificación , Factores de Tiempo , Tos Ferina/prevención & control
10.
Zh Mikrobiol Epidemiol Immunobiol ; (3): 66-70, 1992 Mar.
Artículo en Ruso | MEDLINE | ID: mdl-1509855

RESUMEN

Changes in the histamine-sensitizing activity of whole-cell pertussis vaccine (PV) under the action of immunomodulators (IM) of bacterial (peptide and peptidoglycans), synthetic (peptidoglycan) and vegetable origin have been studied. The study has revealed that these IM, introduced orally and parenterally, exhibit histamine-sensitizing activity, depending on the nature of IM and the optimum selection of the doses of IM and PV.


Asunto(s)
Adyuvantes Inmunológicos/farmacología , Histamina/metabolismo , Vacuna contra la Tos Ferina/farmacología , Animales , Interacciones Farmacológicas , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA
11.
Artículo en Ruso | MEDLINE | ID: mdl-1466170

RESUMEN

The possibility of using monolaurates for removing endotoxin from acellular pertussis vaccines developed at the Laboratory of Immunomodulators, Mechnikov Research Institute for Vaccines and Sera (Moscow), has been studied. Monolaurates PEG-400 and PEG-600 obtained, respectively, from Fluka Chemie AG and Ferak GmbH (Germany) have been used. The use of monolaurate PEG-600 ensures the decrease of the toxicity of acellular pertussis vaccines by 2.34-6.3 times.


Asunto(s)
Lauratos , Vacuna contra la Tos Ferina/aislamiento & purificación , Factores de Virulencia de Bordetella/aislamiento & purificación , Animales , Evaluación Preclínica de Medicamentos , Indicadores y Reactivos , Métodos , Ratones , Vacuna contra la Tos Ferina/inmunología , Vacuna contra la Tos Ferina/toxicidad , Polietilenglicoles , Factores de Virulencia de Bordetella/toxicidad
12.
Artículo en Ruso | MEDLINE | ID: mdl-1466172

RESUMEN

Two vaccine preparations obtained from Bordetella pertussis, whole-cell vaccine constituting one of the components of adsorbed DPT vaccine and acellular vaccine, were tested for mutagenicity. The doses of the preparations covered the range 1-100 ED50. Ames' test and the metaphase analysis of marrow cells of C57BL/6J mice were used. The acellular preparation was also tested on thymectomized mice, taking into consideration chromosomal aberrations in marrow metaphases. Whole-cell and acellular pertussis vaccines did not induce mutations in Salmonella typhimurium and chromosomal aberrations in mouse marrow cells.


Asunto(s)
Mutagénesis/efectos de los fármacos , Vacuna contra la Tos Ferina/toxicidad , Animales , Médula Ósea/efectos de los fármacos , Médula Ósea/ultraestructura , Aberraciones Cromosómicas , Relación Dosis-Respuesta a Droga , Genes Bacterianos/efectos de los fármacos , Masculino , Ratones , Ratones Endogámicos C57BL , Pruebas de Mutagenicidad , Salmonella typhimurium/efectos de los fármacos , Salmonella typhimurium/genética , Vacunas de Productos Inactivados/toxicidad
13.
Artículo en Ruso | MEDLINE | ID: mdl-2552711

RESUMEN

The heterogeneity of the population of B. pertussis laboratory strains with respect to the capacity of individual clones to lyse erythrocytes has been established. The complete or partial reduction of the antigens under study in nonhemolytic bacteria has been shown. The use of hemolytic cultures for the preparation of acellular pertussis vaccine makes it possible to increase the content of B. pertussis toxin in these vaccines.


Asunto(s)
Bordetella pertussis/aislamiento & purificación , Vacuna contra la Tos Ferina/aislamiento & purificación , Antígenos Bacterianos/análisis , Bordetella pertussis/crecimiento & desarrollo , Hemólisis
14.
Artículo en Ruso | MEDLINE | ID: mdl-2904201

RESUMEN

The physicochemical and biological properties of antigenic complexes isolated from the supernatant fluid of the culture medium of B. pertussis, strains 305 and 475, were studied. The preparations obtained from both strains contained proteins, carbohydrates, nucleic acids and lipids. Electrophoresis in polyacrylamide gel revealed the presence of filamentous hemagglutinin, 4 subunits of B. pertussis toxin and agglutinogens in the antigenic complexes of both strains. The preparations of both strains possessed similar toxic properties and, after their detoxification, produced a pronounced protective effect.


Asunto(s)
Antígenos Bacterianos/análisis , Bordetella pertussis/inmunología , Animales , Antígenos Bacterianos/inmunología , Antígenos Bacterianos/aislamiento & purificación , Antígenos Bacterianos/toxicidad , Fenómenos Químicos , Química Física , Cricetinae , Electroforesis en Gel de Poliacrilamida , Inmunización , Ratones , Ratones Endogámicos CBA , Peso Molecular , Tos Ferina/prevención & control
15.
Zh Mikrobiol Epidemiol Immunobiol ; (6): 38-43, 1987 Jun.
Artículo en Ruso | MEDLINE | ID: mdl-2888247

RESUMEN

The biological properties of Bordetella pertussis antigenic complex, obtained by a technologically simple method from the medium used for the cultivation of B. pertussis, were studied. The preparation was characterized by pronounced hemagglutinating activity, toxicity, histamine-sensitizing and leukocytosis-stimulating activity and produced a cytopathogenic effect on the culture of Chinese hamster ovary cells. The detoxified preparations showed pronounced protective activity in experiments on the active and passive protection of mice. The ED50 of the preparation was 0.146 microgram of protein. In the proposed human immunization dose containing 10 micrograms of protein the detoxified preparation showed no hemagglutinating, leukocytosis-stimulating or histamine-sensitizing activity and proved to be nontoxic in the weight loss test on mice.


Asunto(s)
Vacuna contra la Tos Ferina/inmunología , Animales , Antígenos Bacterianos/inmunología , Antígenos Bacterianos/aislamiento & purificación , Antígenos Bacterianos/toxicidad , Bordetella pertussis/inmunología , Células Cultivadas , Relación Dosis-Respuesta Inmunológica , Inmunización , Inmunización Pasiva , Dosificación Letal Mediana , Ratones , Ratones Endogámicos CBA , Vacuna contra la Tos Ferina/aislamiento & purificación , Vacuna contra la Tos Ferina/toxicidad , Factores de Virulencia de Bordetella/toxicidad
16.
Artículo en Ruso | MEDLINE | ID: mdl-3012909

RESUMEN

The influence of extracts from oak bark, St. John's-wort leaves and pine buds on natural immunity characteristics of mice has been studied. The injection of these extracts into mice has been found to enhance their resistance to infection with Staphylococcus aureus and Bordetella pertussis virulent cultures, to decrease the enzymatic activity of 5'-nucleotidase in the peritoneal exudate macrophages of mice and to increase the level of lysozyme in their blood. The action of these extracts has proved to depend on their dosage and the time of observation.


Asunto(s)
Inmunidad Innata/efectos de los fármacos , Extractos Vegetales/uso terapéutico , 5'-Nucleotidasa , Animales , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos , Cobayas , Dosificación Letal Mediana , Macrófagos/enzimología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Muramidasa/sangre , Nucleotidasas/análisis , Plantas Medicinales , Infecciones Estafilocócicas/prevención & control , Factores de Tiempo , Árboles , Tos Ferina/prevención & control
17.
Artículo en Ruso | MEDLINE | ID: mdl-3705806

RESUMEN

The influence of blastolysin, the cell-wall glycopeptide of Lactobacillus bulgaricus, on the main immunobiological properties of pertussis vaccine, its immunogenicity and toxicity, has been studied. The use of blastolysin, introduced as a stimulating agent in definite doses and following definite administration schedules, has been shown to permit achieving not only an increase in the immunogenicity of the corpuscular pertussis vaccine, but also a considerable decrease in its toxicity and histamine-sensitizing activity.


Asunto(s)
Adyuvantes Inmunológicos/uso terapéutico , Antibacterianos , Tos Ferina/inmunología , Animales , Relación Dosis-Respuesta a Droga , Relación Dosis-Respuesta Inmunológica , Evaluación Preclínica de Medicamentos , Interacciones Farmacológicas , Glicopéptidos/uso terapéutico , Histamina/inmunología , Inmunidad/efectos de los fármacos , Inmunización , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Vacuna contra la Tos Ferina/inmunología , Vacuna contra la Tos Ferina/toxicidad , Tos Ferina/prevención & control
18.
Antibiot Med Biotekhnol ; 30(6): 442-6, 1985 Jun.
Artículo en Ruso | MEDLINE | ID: mdl-2998270

RESUMEN

The effect of blastolysin, a glycopeptide of the cell walls of L. bulgaricus, on the resistance of mice to infections caused by S. typhimurium and B. pertussis, lysozyme levels in the blood serum and 5'-nucleotidase activity of peritoneal macrophages was studied. The changes in the nonspecific resistance of mice due to the effect of blastolysin depended on the administration route and the type of the developing infection. It activated the enzymatic activity of the peritoneal macrophages and changed the lysozyme levels in the blood serum of mice. The optimal protective effect of blastolysin was achieved on intramuscular injection in a dose of 100 micrograms.


Asunto(s)
Antibacterianos , Antibióticos Antineoplásicos/farmacología , Inmunidad Innata/efectos de los fármacos , 5'-Nucleotidasa , Animales , Glicopéptidos/farmacología , Macrófagos/enzimología , Ratones , Muramidasa/sangre , Nucleotidasas/metabolismo , Cavidad Peritoneal/citología , Infecciones por Salmonella/inmunología , Salmonella typhimurium , Estimulación Química , Tos Ferina/inmunología
19.
Zh Mikrobiol Epidemiol Immunobiol ; (6): 68-73, 1984 Jun.
Artículo en Ruso | MEDLINE | ID: mdl-6087580

RESUMEN

The reactogenic properties of adsorbed DPT vaccine containing Bordetella pertussis cultures, grown by submerged cultivation, as the pertussis component were studied. The study revealed that the vaccine was low-reactogenic: no severe postvaccinal reactions were recorded in immunized children. The frequency and intensity of febrile and local reactions in children immunized with adsorbed DPT vaccine under trial and the commercial preparation commonly used in the USSR were identical, but considerably less pronounced than in children immunized with foreign vaccines having similar composition.


Asunto(s)
Bordetella pertussis/inmunología , Toxoide Diftérico/inmunología , Vacuna contra la Tos Ferina/inmunología , Toxoide Tetánico/inmunología , Adsorción , Temperatura Corporal/efectos de los fármacos , Ensayos Clínicos como Asunto , Toxoide Diftérico/efectos adversos , Vacuna contra Difteria, Tétanos y Tos Ferina , Combinación de Medicamentos/efectos adversos , Combinación de Medicamentos/inmunología , Humanos , Hiperemia/epidemiología , Hiperemia/etiología , Lactante , Vacuna contra la Tos Ferina/efectos adversos , Toxoide Tetánico/efectos adversos , Factores de Tiempo
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