Asunto(s)
Angiopatía Amiloide Cerebral/diagnóstico , Encefalitis/diagnóstico , Fiebre/etiología , Cefalea/etiología , Adulto , Angiopatía Amiloide Cerebral/complicaciones , Angiopatía Amiloide Cerebral/patología , Diagnóstico Diferencial , Encefalitis/complicaciones , Encefalitis/patología , Humanos , Imagen por Resonancia Magnética , Masculino , Lóbulo Parietal/diagnóstico por imagen , Lóbulo Parietal/patologíaRESUMEN
KANSL2 is an integral subunit of the nonspecific lethal (NSL) chromatin-modifying complex that contributes to epigenetic programs in embryonic stem cells. In this study, we report a role for KANSL2 in regulation of stemness in glioblastoma (GBM), which is characterized by heterogeneous tumor stem-like cells associated with therapy resistance and disease relapse. KANSL2 expression is upregulated in cancer cells, mainly at perivascular regions of tumors. RNAi-mediated silencing of KANSL2 in GBM cells impairs their tumorigenic capacity in mouse xenograft models. In clinical specimens, we found that expression levels of KANSL2 correlate with stemness markers in GBM stem-like cell populations. Mechanistic investigations showed that KANSL2 regulates cell self-renewal, which correlates with effects on expression of the stemness transcription factor POU5F1. RNAi-mediated silencing of POU5F1 reduced KANSL2 levels, linking these two genes to stemness control in GBM cells. Together, our findings indicate that KANSL2 acts to regulate the stem cell population in GBM, defining it as a candidate GBM biomarker for clinical use. Cancer Res; 76(18); 5383-94. ©2016 AACR.
Asunto(s)
Neoplasias Encefálicas/patología , Carcinogénesis/metabolismo , Glioblastoma/patología , Histona Acetiltransferasas/metabolismo , Células Madre Neoplásicas/metabolismo , Adulto , Anciano , Animales , Biomarcadores de Tumor/análisis , Western Blotting , Separación Celular , Femenino , Citometría de Flujo , Técnicas de Silenciamiento del Gen , Xenoinjertos , Humanos , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos NOD , Ratones SCID , Persona de Mediana Edad , Células Madre Neoplásicas/patología , Proteínas Nucleares , Análisis de Secuencia por Matrices de Oligonucleótidos , Reacción en Cadena en Tiempo Real de la Polimerasa , Regulación hacia ArribaRESUMEN
Gerstmann-Sträussler-Scheinker syndrome (GSS) is a dominantly inherited disorder belonging to the group of transmissible human spongiform encephalopathies or prion diseases. Several families affected by GSS with patients carrying mutations in the prion protein gene have been described worldwide. We report clinical, genealogical, neuropathology and molecular study results from two members of the first Argentine kindred affected by GSS. Both family members presented a frontotemporal-like syndrome, one with and the other without ataxia, with different lesions on neuropathology. A Pro to Leu point mutation at codon 102 (P102L) of the prion protein gene was detected in one of the subjects studied. The pathogenic basis of phenotypic variability observed in this family remains unclear, but resembles that observed in other P102L GSS patients from the same family.
Asunto(s)
Enfermedad de Gerstmann-Straussler-Scheinker/diagnóstico , Enfermedad de Gerstmann-Straussler-Scheinker/genética , Priones/genética , Adulto , Encéfalo/patología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Mutación , Linaje , Fenotipo , Proteínas PriónicasRESUMEN
Most of the mutations in the presenilin-1 gene (PS-1) are associated with familial Alzheimer's disease (AD). However, certain examples can be associated with frontotemporal dementia (FTD). We performed a clinical evaluation of individuals belonging to a family with the FTD phenotype, and additional molecular studies and neuropathological assessment of the proband. The PS-1 M146V mutation was found in the 50-year-old subject (the proband) with family history of early-onset FTD. Neuropathological examination showed abundant amyloid plaques, widespread neurofibrillary pathology, Pick bodies in the hippocampus and cortex, cortical globose tangles and ubiquitin-positive nuclear inclusions in white matter oligodendrocytes. We report a kindred with clinical features of FTD, whose proband bore the PS-1 M146V mutation and showed diffuse Alzheimer's type pathology and Pick bodies on post-mortem neuropathological examination. As with other mutations within the same codon, this substitution may predispose to both diseases by affecting APP and/or tau processing.
Asunto(s)
Demencia/genética , Demencia/patología , Salud de la Familia , Lóbulo Frontal/patología , Presenilina-1/genética , Lóbulo Temporal/patología , Adulto , Análisis Mutacional de ADN , Electroencefalografía , Femenino , Humanos , Cuerpos de Inclusión/genética , Cuerpos de Inclusión/patología , Imagen por Resonancia Magnética , Masculino , Metionina/genética , Persona de Mediana Edad , Pruebas Neuropsicológicas , Placa Amiloide/patología , Tomógrafos Computarizados por Rayos X , Valina/genéticaRESUMEN
In Rosai-Dorfman disease (RDD), exclusive extranodal involvement with lesions limited to the kidneys is very uncommon and has been described only in adult patients. Occasionally, human herpesvirus 6 (HHV-6) has also been detected in RDD tissue samples. We present the case of a 7-year-old boy referred to our center presenting a single solid mass in the right kidney measuring 3.4 cm, detected both on contrast computed tomography and magnetic resonance imaging. Surgical excision was successfully completed, and the pathology report informed characteristic histopathology and immmunohistochemistry features of RDD. Human herpesvirus 6 was detected and amplified by polymerase chain reaction, as well as by immunohistochemistry. We discuss imaging and histology-based differential diagnoses in the pediatric age group. Although RDD is a rare histiocytic disorder of unknown etiology and pathogenesis, the presence of HHV-6 observed in this case supports the possibility of an abnormal immunologic response linked to viral presence.
Asunto(s)
Herpesvirus Humano 6/aislamiento & purificación , Histiocitosis Sinusal/diagnóstico , Enfermedades Renales/diagnóstico , Infecciones por Roseolovirus/diagnóstico , Niño , ADN Viral/análisis , Diagnóstico Diferencial , Herpesvirus Humano 6/genética , Histiocitosis Sinusal/cirugía , Histiocitosis Sinusal/virología , Humanos , Inmunohistoquímica , Riñón/diagnóstico por imagen , Riñón/patología , Enfermedades Renales/cirugía , Enfermedades Renales/virología , Imagen por Resonancia Magnética , Masculino , Reacción en Cadena de la Polimerasa , Infecciones por Roseolovirus/cirugía , Infecciones por Roseolovirus/virología , Tomografía Computarizada por Rayos X , Resultado del TratamientoRESUMEN
The Baltimore Longitudinal Study of Aging (BLSA) was established in 1958 and is one the oldest prospective studies of aging in the USA and the world. The BLSA is supported by the National Institute of Aging (NIA) and its mission is to learn what happens to people as they get old and how to sort out changes due to aging from those due to disease or other causes. In 1986, an autopsy program combined with comprehensive neurologic and cognitive evaluations was established in collaboration with the Johns Hopkins University Alzheimer's Disease Research Center (ADRC). Since then, 211 subjects have undergone autopsy. Here we review the key clinical neuropathological correlations from this autopsy series. The focus is on the morphological and biochemical changes that occur in normal aging, and the early neuropathological changes of neurodegenerative diseases, especially Alzheimer's disease (AD). We highlight the combined clinical, pathologic, morphometric, and biochemical evidence of asymptomatic AD, a state characterized by normal clinical evaluations in subjects with abundant AD pathology. We conclude that in some individuals, successful cognitive aging results from compensatory mechanisms that occur at the neuronal level (i.e., neuronal hypertrophy and synaptic plasticity) whereas a failure of compensation may culminate in disease.
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Envejecimiento/fisiología , Encéfalo/patología , Demencia/epidemiología , Demencia/patología , Anciano , Anciano de 80 o más Años , Enfermedad de Alzheimer/epidemiología , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/metabolismo , Baltimore/epidemiología , Encéfalo/metabolismo , Demencia/metabolismo , Femenino , Estudios de Seguimiento , Humanos , Masculino , Enfermedad de Parkinson/epidemiología , Enfermedad de Parkinson/metabolismo , Enfermedad de Parkinson/patología , Donantes de Tejidos , Proteínas tau/metabolismoAsunto(s)
Síndrome Acrocallosal/patología , Neoplasias Encefálicas/patología , Lipoma/patología , Síndrome Acrocallosal/complicaciones , Neoplasias Encefálicas/complicaciones , Neoplasias Encefálicas/cirugía , Preescolar , Humanos , Lactante , Lipoma/complicaciones , Lipoma/cirugía , Imagen por Resonancia Magnética , Masculino , Neoplasias Primarias Múltiples/patología , Neoplasias Primarias Múltiples/cirugía , Neoplasias de los Tejidos Blandos/patologíaRESUMEN
We describe a 60 year-old woman presenting with visual loss of her left eye. No lymphadenopathies, fever, or weight loss were detected. Neuroimaging studies revealed an extra-axial mass along the posterior aspect of the left optic nerve. The mass was resected and showed xanthomatous histiocytes that were positive for CD-68, occasionally positive for S-100, and negative for CD-1. The lesion was diagnosed as Erdheim-Chester disease (ECD) affecting the CNS. The patient is under systemic evaluation in order to discover other ECD lesions. Microscopic findings and differential diagnoses are discussed.
Asunto(s)
Ceguera/etiología , Enfermedad de Erdheim-Chester/diagnóstico , Histiocitos/patología , Neoplasias del Nervio Óptico/diagnóstico , Antígenos CD/análisis , Antígenos de Diferenciación Mielomonocítica/análisis , Diagnóstico Diferencial , Enfermedad de Erdheim-Chester/complicaciones , Enfermedad de Erdheim-Chester/metabolismo , Femenino , Histiocitos/química , Humanos , Imagen por Resonancia Magnética , Persona de Mediana Edad , Neoplasias del Nervio Óptico/complicaciones , Neoplasias del Nervio Óptico/metabolismo , Proteínas S100/análisisRESUMEN
Alzheimer disease is the most common dementia in older Americans, but its impact on blacks is not clearly understood. We examined prospectively 200 autopsy brains at the Office of the Chief Medical Examiner in Maryland and compared the frequency and severity of Alzheimer lesions in blacks and whites. Histologic sections of the hippocampus and entorhinal and neocortices were immunostained for Abeta and tau proteins. Subjects were genotyped for ApoE. Abeta deposits were rated as none, sparse, moderate, or frequent; tau lesions were rated into 4 groups corresponding to Braak scores; and Abeta angiopathy was classified as present or absent. Outcome scores were treated as ordinal variables and analyzed by proportional odds logistic regression. Abeta plaques were present in 60% of black males, 58% of white males, 74% of black females, and 74% of white females. Tau lesions were present in 96% of black males, 88% of white males, 96% of black females, and 96% of white females. Neither race nor gender was a significant factor in the frequency or severity of Alzheimer lesions, and ApoE4 increased the risk for Alzheimer lesions similarly in blacks and whites.