Bacteriophage-based cocktail modulates selected immunological parameters and post-challenge survival of rainbow trout (Oncorhynchus mykiss).

Recently, a rapid increase in the resistance of pathogenic bacteria to antibiotics and chemotherapeutics admitted for use in aquaculture has been observed. This happens especially often in intensive breeding. The use of drugs in closed circuits is problematic because it can damage biological filters. Therefore, in recent years, there has been a growing interest in natural methods of combating pathogens. These include bacteriophages. The aim of the study was to determine the safety of the new BAFADOR® bacteriophage-based preparation, its effect on selected immunological parameters and the effectiveness of prophylactic and therapeutic use after experimental infections with pathogenic bacteria Aeromonas hydrophila and Pseudomonas fluorescens. The use of BAFADOR® increased the activity of lysozyme, total protein level and immunoglobulin level. The level of ceruloplasmin in the rainbow trout serum remained unchanged regardless of the route of administration of the preparation. Potential killing activity and metabolic activity of spleen phagocytes and proliferation of pronephros lymphocytes were higher compared to the control group. Both therapeutic and prophylactic application of the preparation after mixed experimental infection of A. hydrophila and P. fluorescens limited the mortality of rainbow trout.

Influence of bacteriophages cocktail on European eel (Anguilla anguilla) immunity and survival after experimental challenge.

Inland fishery belongs to those branches of animal production that use very large amounts of chemotherapeutics, in particular antibiotics. The accumulation of chemotherapeutic agents in bottom sediments is a direct threat to the aquatic environment and directly affects the condition and health of the fish. Finding a preparation that could be used both prophylactically to increase the resistance of fish and therapeutically in case of infection with pathogenic bacteria, without side effects for fish and aquatic environment could be a great solution to this problem. Our aim was to determine influence of BAFADOR® the new bacteriophage-based preparation on European eel immunity and survival after experimental challenge. Application of BAFADOR® increased total protein level, immunoglobulin level, lysozyme activity and ceruloplasmin level in European eel serum. Potential killing activity and metabolic activity of spleen phagocytes as well as pronephros lymphocyte proliferation of was higher compared to control. The preparation also reduced mortality after experimental infections with the pathogenic bacteria Aeromonas hydrophila and Pseudomonas fluorescens. Our results showed that preparation BAFADOR® is well tolerated by the fish organism causing stimulation of cellular and humoral immunity parameters and reduces the mortality of the European eel after experimental challenge.

Influence of ß-glucan Leiber(®)Beta-S on selected innate immunity parameters of European eel (Anguilla anguilla) in an intensive farming system.

Nutritional support plays an important role in promoting high cellular and humoral innate immunity activity and in preventing outbreaks of disease. The effects of ß-glucan Leiber(®)Beta-S dietary supplementation on selected nonspecific immune parameters in juvenile European eel (Anguilla anguilla) in an intensive culture system were studied. The fish were fed commercial pellets containing either 0 (control group) or 200 mg Leiber(®)Beta-S kg-1 of feed (glucan-fed group). After four and eight weeks of feeding, the levels of the following immunological parameters were measured: phagocyte respiratory burst activity, phagocyte potential killing activity, lymphocyte proliferation stimulated by concanavaline A or lipopolysaccharide, serum lysozyme activity, and total immunoglobulin (Ig) serum levels. After four and eight weeks of feeding 200 mg Leiber(®)Beta-S kg feed-1 the levels of all immune parameters were statistically significantly higher (p < 0.05) in the glucan-treated group than in the control group. After eight weeks of feeding the fish 200 mg Leiber(®)Beta-S kg feed-1 and after an additional eight weeks in ponds, the levels of all immune parameters, excluding lymphocyte proliferation stimulated by concanavaline A, were statistically significantly higher (p < 0.05) in the glucan-fed group than in the control group. These data suggest that feeding juvenile eel Leiber(®)Beta-S for four and eight weeks might improve innate immunity.

Mercury toxicity and the protective role of selenium in eel, Anguilla anguilla.

The aim of the study was to determine the impact trace metals, mainly toxic ones, on the condition of eel (Anguilla anguilla) inhabiting four regions of Poland. Metal concentrations in eel muscle tissues were studied as functions of size, region, and season 2011-2012. The levels of metals were also used for risk assessment on consumer health. Copper and zinc occurred at concentrations that could only have positive impacts on eel condition. Low levels of cadmium and lead did not impair the condition of the fish. However, mercury occurred at high levels and increased with fish length and season. The mercury levels in eels were compared with the threshold of toxicity (500-1,200 µg kg(-1)), which can cause changes in biochemical processes and impair fish reproduction. The concentration of mercury was 1,010 µg kg(-1) in one specimen of the 120 samples examined, and in 16 specimens, it exceeded 500 µg kg(-1). The toxic effects of the mercury could have been attenuated by the selenium in the muscles of the eel, especially in the muscles of smaller specimens in which the Se/Hg molar ratio was higher than 1 with a positive correlation between these two elements. In larger specimens measuring in excess of 70 cm, this coefficient was below 1, and the mercury to selenium correlation was negative, which meant that the protective effects of selenium were weaker. The mercury in the muscles of large specimens at levels exceeding 500 µg kg(-1) could have weakened eel condition and also posed a threat to consumer health. The cadmium and lead in the muscles of the eel did not affect the condition of the fish. Mercury weakened the condition of large eel, A. anguilla. Selenium protected small- and medium-sized eel against the toxic effects of mercury.

The occurrence of organic contaminants in European eel (Anguilla anguilla) in Poland: an environmental quality assessment.

The objective of the present study was to provide information on the levels of pollutants in the tissues of eels caught in Polish waters. The contaminants included in the study are those which have not yet been widely studied in eel stocks, but which arouse concern in relation to the environment. An overview of the pollutant levels in eels caught in other European waters was also conducted. The results are evaluated in terms of environmental quality and consumer health. The mean concentrations of ΣPBDEs and ΣHBCDs in muscles of eels sampled in Polish waters were between 1 and 2 ng g(-1) ww. The mean TBT concentrations were between 2 and 4 ng g(-1)ww with the exception of samples from the Szczecin Lagoon, in which the mean TBT concentration was about tenfold higher.