FBG array based wavelength calibration scheme for Fourier domain mode-locked laser with pm resolution and hourly stability.

We demonstrate a fiber Bragg grating (FBG) array based wavelength calibration scheme for Fourier domain mode-locked (FDML) laser. The wavelength interval and the temperature feedback module of the FBG array are designed to ensure the reference stability of the wavelength calibration scheme. Combined with the calibration scheme, the FDML laser with a tunable wavelength range of ∼60 nm, a center wavelength of 1300 nm and a sweep frequency of 39.63 kHz is built up to demonstrate its feasibility. The FBG wavelength demodulation based on the calibrated FDML laser system shows a wavelength resolution of 2.76 pm and hourly stability of 10.22 pm.

Novel Method to Assess Endothelial Function via Monitoring of Perfusion Response to Local Heating by Imaging Photoplethysmography.

Endothelial dysfunction is one of the most important markers of the risk of cardiovascular complications. This study is aimed to demonstrate the feasibility of imaging photoplethysmography to assess microcirculation response to local heating in order to develop a novel technology for assessing endothelial function. As a measure of vasodilation, we used the relative dynamics of the pulsatile component of the photoplethysmographic waveform, which was assessed in a large area of the outer surface of the middle third of the subject's forearm. The perfusion response was evaluated in six healthy volunteers during a test with local skin heating up to 40-42 °C and subsequent relaxation. The proposed method is featured by accurate control of the parameters affecting the microcirculation during the prolonged study. It was found that in response to local hyperthermia, a multiple increase in the pulsation component, which has a biphasic character, was observed. The amplitude of the first phase of the perfusion reaction depends on both the initial skin temperature and the difference between the basal and heating temperatures. The proposed method allows the assessment of a reproducible perfusion increase in response to hyperthermia developed due to humoral factors associated with the endothelium, thus allowing detection of its dysfunction.

Non-steady-state photo-EMF in ß-Ga2O3 crystals at λ = 457 nm.

The non-steady-state photoelectromotive force is excited in a monoclinic gallium oxide crystal at wavelength λ = 457 nm. The crystal grown in an oxygen atmosphere is insulating and highly transparent for a visible light, nevertheless, the formation of dynamic space-charge gratings and observation of the photo-EMF signal is achieved without application of any electric field to the sample. The dependencies of the signal amplitude on the frequency of phase modulation, light intensity, spatial frequency and light polarization are measured. The material demonstrates the anisotropy along the [100] and [010] directions, namely, there is a small difference in the transport parameters and a pronounced polarization dependence of the signal. The crystal's photoconductivity, responsivity and diffusion length of electrons are estimated for the chosen light wavelength and compared with the ones for other wide-bandgap crystals.

Animal model of assessing cerebrovascular functional reserve by imaging photoplethysmography.

Assessment of the cerebral blood-flow-reserve in patients with cerebrovascular diseases is extremely important in terms of making prognosis, determining treatment tactics, and controlling the revascularization outcome in the case of reconstructive interventions on the brain vessels. However, there is no easy-to-use, contactless method for either assessing the functional reserve of the cortical vascular network or intraoperative monitoring of surgical intervention. Our study aims to demonstrate feasibility of green-light imaging photoplethysmography (iPPG) to estimate cerebrovascular functional reserve in animal model of craniosurgical intervention. Custom-made iPPG system was exploited to visualize intracranial vessels in anesthetized Wistar rats (n = 15). Video frames of rat's cortex were recorded concurrently with systemic blood pressure, end-tidal CO2, and electrocardiogram. We found that injection of dorzolamide (carbonic-anhydrase inhibitor) significantly increased the blood-pulsations amplitude in all animals by 35 ± 19% (p < 0.001). Such an increase negatively correlated with significant decrease in end-tidal CO2 by 32 ± 7% (p < 0.001). It is noteworthy that the dorzolamide injection did not lead to significant changes in systemic blood pressure. Concluding, pulsations amplitude is a marker of the vascular tone that can be used to evaluate the functional cerebrovascular reserve. Imaging PPG is a simple and convenient method to assess cerebral blood flow, including during various neurosurgical interventions.

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends.

Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

Theoretical Study of Electrochemical and Electrochromic Properties of Novel Viologen Derivatives: Effects of Donors and π-Conjugation.

We propose a linkage approach by merging ambipolar electrochromic (EC) materials in both π-acceptor-π (π-A-π) and donor-acceptor-donor (D-A-D) configurations and investigated their electrochemical and spectroelectrochemical properties using density functional theory calculations. Here, we considered anthracene, toluene, and pyrene as π-conjugated molecules, triphenylamine (TPA) as a donor, and viologen as an acceptor moiety for π-A-π and D-A-D configurations. We have also explored the substitutional effects in the donor moiety on the overall electrochromism during both oxidation and reduction processes. Here, we mainly focused on the relationship between the structure, substitution of functional groups, electronic and spectral properties, as well as redox potential of the designed monomers. Our calculations indicate that the designed monomers have attractive absorption properties and show clear color switching upon the redox process. We find that the substitution of stronger electron-donating and π-spacer groups create new absorption peaks in the oxidation states. These designed viologen derivatives will be potential candidates, which can be used in both oxidation and reduction processes for upcoming EC devices.

Origin of Infrared Light Modulation in Reflectance-Mode Photoplethysmography.

We recently pointed out the important role of dermis deformation by pulsating arterial pressure in the formation of a photoplethysmographic signal at green light. The aim of this study was to explore the role of this novel finding in near-infrared (NIR) light. A light-emitting diode (LED)-based imaging photoplethysmography (IPPG) system was used to detect spatial distribution of blood pulsations under frame-to-frame switching green and NIR illumination in the palms of 34 healthy individuals. We observed a significant increase of light-intensity modulation at the heartbeat frequency for both illuminating wavelengths after a palm was contacted with a glass plate. Strong positive correlation between data measured at green and NIR light was found, suggesting that the same signal was read independently from the depth of penetration. Analysis of the data shows that an essential part of remitted NIR light is modulated in time as a result of elastic deformations of dermis caused by variable blood pressure in the arteries. Our observations suggest that in contrast with the classical model, photoplethysmographic waveform originates from the modulation of the density of capillaries caused by the variable pressure applied to the skin from large blood vessels. Particularly, beat-to-beat transmural pressure in arteries compresses/decompresses the dermis and deforms its connective-tissue components, thus affecting the distance between the capillaries, which results in the modulation of absorption and scattering coefficients of both green and NIR light. These findings are important for the correct interpretation of this widely used medical technique, which may have novel applications in diagnosis and treatment monitoring of aging and skin diseases.

Influence of a skin status on the light interaction with dermis.

We present experimental evidence that the parameters of green light remitted from a human tissue in-vivo strongly depend on skin contact status. In case when the skin is free of any contact, simultaneous recording of imaging photoplethysmogram (iPPG) and electrocardiogram revealed that contactless iPPG fails in correct estimates of the heart rate in almost half of the cases. Meanwhile, the number of successful correlations between ECG and iPPG is significantly increased when the skin is in contact with a glass plate. These observations are in line with the recently proposed model in which pulsatile arteries deform the connective-tissue components of the dermis thus resulting in temporal modulation of the capillary density interacting with slightly penetrating light.

A new look at the essence of the imaging photoplethysmography.

Photoplethysmography (PPG) is a noninvasive optical method accepted in the clinical use for measurements of arterial oxygen saturation. It is widely believed that the light intensity after interaction with the biological tissue in vivo is modulated at the heartbeat frequency mainly due to pulsatile variations of the light absorption caused by arterial blood-volume pulsations. Here we report experimental observations, which are not consistent with this model and demonstrate the importance of elastic deformations of the capillary bed in the formation of the PPG waveform. These results provide new insight on light interaction with live tissue. To explain the observations we propose a new model of PPG in which pulse oscillations of the arterial transmural pressure deform the connective-tissue components of the dermis resulting in periodical changes of both the light scattering and absorption. These local changes of the light-interaction parameters are detected as variations of the light intensity returned to a photosensitive camera. Therefore, arterial pulsations can be indirectly monitored even by using the light, which slightly penetrates into the biological tissue.

Fast photogalvanic response of a Bi12SiO20 crystal.

A photoelectric response of a Bi12SiO20 crystal grown in an argon atmosphere on a linearly polarized light (which is referred to as the linear photogalvanic effect) is reported for the first time in the nanosecond-time domain. Optimal geometry for detection of the photo-induced current concerning the orientation of the polarization state of the incident light in respect to the crystallographic axes of a sample was determined considering both the natural optical activity and light absorption of sillenite crystals. Spectral dependence of the photogalvanic current was measured in the visible part (410 - 610 nm) of the spectrum. Temporal shape of light-induced electric-current pulses observed at different experimental conditions is discussed. Obtained results are believed to show that sillenite crystals are very prospective for development of different ultra-fast optoelectronics devices.

Fiber sensors multiplexing using vectorial wave mixing in a photorefractive crystal.

We propose a novel multiplexing system for sensing of dynamic strains excited in different multimode optical fibers. Multiplexing of the sensors is implemented by using vectorial wave mixing technique in the reflection geometry of hologram formation in a photorefractive crystal of CdTe:V. We analyzed different mechanisms of the crosstalk between measuring channels and showed that system performance is strongly affected by residual stresses of the photorefractive crystal.

Sensing of multimode-fiber strain by a dynamic photorefractive hologram.

We present a strain sensor in which a multimode fiber is used as a sensitive element. High sensitivity to dynamic strains is achieved by means of vectorial wave mixing in a photorefractive CdTe:V crystal. It was found that the largest source of noise in our sensor is related to the instability of the polarization state of speckles emerging from the fiber. This noise is significantly diminished in fiber with a core of large diameter (550 microm).

Fast adaptive interferometer on dynamic reflection hologram in CdTe:V.

We present an adaptive interferometer based on the reflection dynamic hologram recorded in photorefractive CdTe:V crystal with no external electric field. Linear phase-to-intensity transformation is achieved by vectorial mixing of two waves with different polarization states (linear and elliptical) in the anisotropic diffraction geometry. Comparison of reflection and transmission geometries considering both sensitivity and adaptability is carried out. It is shown that the reflection geometry is characterized by better combination of these parameters provided that the crystal possesses high enough concentration of photorefractive centers.