RESUMEN
Cutaneous sclerotic chronic graft-versus-host disease (cGVHD) is a common and highly morbid complication of allogeneic hematopoietic stem cell transplantation. Our goals were to identify signals active in the skin of patients with sclerotic cGVHD in an effort to better understand how to treat this manifestation and to explore the heterogeneity of the disease. We identified genes that are significantly upregulated in the skin of patients with sclerotic cGVHD (n = 17) compared with those in the skin of patients who underwent allogeneic hematopoietic stem cell transplantation without cutaneous cGVHD (n = 9) by bulk RNA sequencing. Sclerotic cGVHD was most associated with T helper 1, phagocytic, and fibrotic pathways. In addition, different transcriptomic groups of affected patients were discovered: those with fibrotic and inflammatory/T helper 1 gene expression (the fibroinflammatory group) and those with predominantly fibrotic/TGFß-associated expression (the fibrotic group). Further study will help elucidate whether these gene expression findings can be used to tailor treatment decisions. Multiple proteins encoded by highly induced genes in the skin (SFRP4, SERPINE2, COMP) were also highly induced in the plasma of patients with sclerotic cGVHD (n = 16) compared with those in plasma of control patients who underwent allogeneic hematopoietic stem cell transplantation without sclerotic cGVHD (n = 17), suggesting these TGFß and Wnt pathway mediators as candidate blood biomarkers of the disease.
Asunto(s)
Fármacos Dermatológicos , Psoriasis , Enfermedades Cutáneas Vesiculoampollosas , Humanos , Proteína Antagonista del Receptor de Interleucina 1/efectos adversos , Psoriasis/tratamiento farmacológico , Fármacos Dermatológicos/uso terapéutico , Enfermedades Cutáneas Vesiculoampollosas/tratamiento farmacológicoRESUMEN
Although autoimmune manifestations can be associated with various lymphomas, they are distinctly unusual with primary cutaneous anaplastic large cell lymphoma (PCALCL). We present the case of a 76-year-old woman who, over the course of 2 years, presented with a variety of autoimmune disorders including minimal change disease and focal glomerulosclerosis, visual loss due to retinal vasculitis, immune mediated thrombocytopenia, autoimmune hemolytic anemia, and inflammatory polyarthritis in conjunction with elevated rheumatoid factor, cryoglobulins, and hypocomplementemia. She ultimately developed PCALCL that took an aggressive course and to which she ultimately succumbed. Our case adds to the growing literature demonstrating autoimmune manifestations associated with a variety of lymphoid malignancies. We propose that immune dysregulation can predispose patients to the development of both autoimmune and lymphoproliferative disease. Suspicion of underlying malignancy in patients presenting with otherwise unexplained autoimmune phenomena may prompt earlier diagnosis.
Asunto(s)
Linfoma Anaplásico Cutáneo Primario de Células Grandes , Neoplasias Cutáneas , Anciano , Femenino , HumanosRESUMEN
Chronic graft-versus-host disease (GVHD) can be associated with significant morbidity, in part because of nonreversible fibrosis, which impacts physical functioning (eye, skin, lung manifestations) and mortality (lung, gastrointestinal manifestations). Progress in preventing severe morbidity and mortality associated with chronic GVHD is limited by a complex and incompletely understood disease biology and a lack of prognostic biomarkers. Likewise, treatment advances for highly morbid manifestations remain hindered by the absence of effective organ-specific approaches targeting "irreversible" fibrotic sequelae and difficulties in conducting clinical trials in a heterogeneous disease with small patient numbers. The purpose of this document is to identify current gaps, to outline a roadmap of research goals for highly morbid forms of chronic GVHD including advanced skin sclerosis, fasciitis, lung, ocular and gastrointestinal involvement, and to propose strategies for effective trial design. The working group made the following recommendations: (1) Phenotype chronic GVHD clinically and biologically in future cohorts, to describe the incidence, prognostic factors, mechanisms of organ damage, and clinical evolution of highly morbid conditions including long-term effects in children; (2) Conduct longitudinal multicenter studies with common definitions and research sample collections; (3) Develop new approaches for early identification and treatment of highly morbid forms of chronic GVHD, especially biologically targeted treatments, with a special focus on fibrotic changes; and (4) Establish primary endpoints for clinical trials addressing each highly morbid manifestation in relationship to the time point of intervention (early versus late). Alternative endpoints, such as lack of progression and improvement in physical functioning or quality of life, may be suitable for clinical trials in patients with highly morbid manifestations. Finally, new approaches for objective response assessment and exploration of novel trial designs for small populations are required.
Asunto(s)
Enfermedad Injerto contra Huésped , Enfermedad Crónica , Consenso , Humanos , Incidencia , National Institutes of Health (U.S.) , Calidad de Vida , Estados UnidosAsunto(s)
Interacciones Huésped-Patógeno , Infecciones por Polyomavirus/complicaciones , Poliomavirus/aislamiento & purificación , Prurito/etiología , Infecciones Tumorales por Virus/complicaciones , Adulto , Femenino , Humanos , Inmunidad Innata , Infecciones por Polyomavirus/inmunología , Prurito/inmunología , Prurito/virologíaRESUMEN
Graft-versus-host disease (GVHD) is a multisystemic disorder that affects 30%-80% of patients who undergo allogeneic hematopoietic stem cell transplantation 10%-15% of GVHD patients develop sclerotic features affecting the skin or deeper tissues, leading to functional limitations and poor quality of life. There is limited literature regarding the indications and efficacy of specific rehabilitative interventions in sclerotic GVHD (sclGVHD). In this article, we summarize the current evidence supporting rehabilitation intervention in sclGVHD and offer our approach to the multidisciplinary management of this disease. In addition, we review techniques that have been employed in other sclerotic skin diseases (eg, iontophoresis, extracorporeal shock waves, botulinum toxin A, adipose derived stromal vascular fraction), but that require further validation in the sclGVHD setting. Ultimately, optimal care for this complex disease requires a multidisciplinary approach that includes a rehabilitation and adaptive program tailored to each patient's needs.
Asunto(s)
Enfermedad Injerto contra Huésped/rehabilitación , Trasplante de Células Madre Hematopoyéticas , Terapia Ocupacional , Grupo de Atención al Paciente , Modalidades de Fisioterapia , Enfermedades de la Piel/rehabilitación , Fascia/patología , Humanos , Calidad de Vida , EsclerosisRESUMEN
Human parvovirus B19 (B19V) is well known for its infectivity. However, the risk for communicability to previously unexposed healthcare professionals is controversial. We report here a small outbreak of B19V infection among physicians and family members in an adult rheumatology practice that occurred after providing care for a patient with B19V arthropathy. As B19V-infected patients who demonstrate findings of erythema infectiosum or viral arthritis are generally beyond the period of transmissability, strict handwashing and droplet precautions remain imperative when there is contact with potentially pre-symptomatic family members.
Asunto(s)
Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Eritema Infeccioso/transmisión , Familia , Transmisión de Enfermedad Infecciosa de Paciente a Profesional , Adulto , Diagnóstico Diferencial , Eritema Infeccioso/diagnóstico , Eritema Infeccioso/virología , Femenino , Humanos , Parvovirus B19 HumanoAsunto(s)
Calcinosis/etiología , Enfermedad Injerto contra Huésped/complicaciones , Enfermedades de la Piel/etiología , Adolescente , Adulto , Anticuerpos Antinucleares/sangre , Calcinosis/sangre , Enfermedad Crónica , Femenino , Enfermedad Injerto contra Huésped/sangre , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Enfermedades de la Piel/sangre , Trasplante de PielAsunto(s)
Enfermedades del Cabello/virología , Trasplante de Riñón/efectos adversos , Infecciones por Polyomavirus/virología , Antivirales/uso terapéutico , Biopsia , Femenino , Enfermedades del Cabello/diagnóstico , Enfermedades del Cabello/tratamiento farmacológico , Enfermedades del Cabello/inmunología , Humanos , Huésped Inmunocomprometido , Inmunosupresores/efectos adversos , Persona de Mediana Edad , Infecciones por Polyomavirus/diagnóstico , Infecciones por Polyomavirus/tratamiento farmacológico , Infecciones por Polyomavirus/inmunología , Resultado del TratamientoRESUMEN
Allergic diseases represent a significant burden in industrialized countries, but why and how the immune system responds to allergens remain largely unknown. Because many clinically significant allergens have proteolytic activity, and many helminths express proteases that are necessary for their life cycles, host mechanisms likely have evolved to detect the proteolytic activity of helminth proteases, which may be incidentally activated by protease allergens. A cysteine protease, papain, is a prototypic protease allergen that can directly activate basophils and mast cells, leading to the production of cytokines, including IL-4, characteristic of the type 2 immune response. The mechanism of papain's immunogenic activity remains unknown. Here we have characterized the cellular response activated by papain in basophils. We find that papain-induced IL-4 production requires calcium flux and activation of PI3K and nuclear factor of activated T cells. Interestingly, papain-induced IL-4 production was dependent on the immunoreceptor tyrosine-based activation motif (ITAM) adaptor protein Fc receptor γ-chain, even though the canonical ITAM signaling was not activated by papain. Collectively, these data characterize the downstream signaling pathway activated by a protease allergen in basophils.
Asunto(s)
Alérgenos/farmacología , Basófilos/metabolismo , Interleucina-4/biosíntesis , Papaína/farmacología , Transducción de Señal/efectos de los fármacos , Subunidades del Complejo de Proteínas Adaptadoras/fisiología , Animales , Basófilos/efectos de los fármacos , Basófilos/inmunología , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/fisiología , Señalización del Calcio/efectos de los fármacos , Señalización del Calcio/inmunología , Células Cultivadas , Inhibidores de Cisteína Proteinasa/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Inmunización , Interleucina-13/biosíntesis , Interleucina-13/genética , Interleucina-33 , Interleucina-4/genética , Interleucinas/farmacología , Leucina/análogos & derivados , Leucina/farmacología , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Sistema de Señalización de MAP Quinasas/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Noqueados , Factores de Transcripción NFATC/metabolismo , Papaína/inmunología , Fosfatidilinositol 3-Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-akt/fisiología , Receptores de IgE/genética , Receptores de IgE/fisiología , Receptores de IgG/genética , Receptores de IgG/fisiología , Transducción de Señal/inmunología , Organismos Libres de Patógenos EspecíficosRESUMEN
At the intersection of atopy and autoimmunity, we present a patient with seronegative rheumatoid arthritis (RA) who developed hypereosinophilia, without evidence of other etiologies, as she became rheumatoid factor (RF) positive. Although the magnitude of eosinophilia in patients with RA has been thought to reflect the severity or activity of the RA, in our patient, eosinophilia developed at a time when the patient's synovitis was well controlled. Although eosinophilia may reflect associated drug hypersensitivity, discontinuation of the medications utilized to control our patient's disease, adalimumab and methotrexate, did not promote clinical improvement. Probably the most curious aspect of our patient was the concomitant development of rheumatoid factor seropositivity in the setting of previously seronegative RA. The temporal relationship between the development of peripheral eosinophilia and seroconversion suggests a possible connection between these events. We speculate that the T cell cytokine production that can induce eosinophilia may simultaneously activate RF production.
Asunto(s)
Artritis Reumatoide/complicaciones , Autoinmunidad/inmunología , Síndrome Hipereosinofílico/complicaciones , Adalimumab , Anciano de 80 o más Años , Anticuerpos Monoclonales Humanizados/uso terapéutico , Antirreumáticos/uso terapéutico , Artritis Reumatoide/tratamiento farmacológico , Artritis Reumatoide/inmunología , Femenino , Humanos , Síndrome Hipereosinofílico/inmunología , Metotrexato/uso terapéutico , Factor Reumatoide/inmunologíaRESUMEN
Diverse cellular responses to external cues are controlled by a small number of signal-transduction pathways, but how the specificity of functional outcomes is achieved remains unclear. Here we describe a mechanism for signal integration based on the functional coupling of two distinct signaling pathways widely used in leukocytes: the ITAM pathway and the Jak-STAT pathway. Through the use of the receptor for interferon-γ (IFN-γR) and the ITAM adaptor Fcγ as an example, we found that IFN-γ modified responses of the phagocytic antibody receptor FcγRI (CD64) to specify cell-autonomous antimicrobial functions. Unexpectedly, we also found that in peritoneal macrophages, IFN-γR itself required tonic signaling from Fcγ through the kinase PI(3)K for the induction of a subset of IFN-γ-specific antimicrobial functions. Our findings may be generalizable to other ITAM and Jak-STAT signaling pathways and may help explain signal integration by those pathways.
Asunto(s)
Motivo de Activación del Inmunorreceptor Basado en Tirosina/inmunología , Janus Quinasa 2/metabolismo , Listeriosis/inmunología , Macrófagos/inmunología , Receptor Cross-Talk/inmunología , Factor de Transcripción STAT1/metabolismo , Animales , Carga Bacteriana , Células Cultivadas , Fragmentos Fc de Inmunoglobulinas/genética , Fragmentos Fc de Inmunoglobulinas/metabolismo , Motivo de Activación del Inmunorreceptor Basado en Tirosina/genética , Interferón gamma/inmunología , Interferón gamma/metabolismo , Janus Quinasa 2/genética , Ratones , Ratones Endogámicos , Ratones Noqueados , Óxido Nítrico Sintasa de Tipo II/genética , Óxido Nítrico Sintasa de Tipo II/metabolismo , Fagocitosis/genética , Inhibidores de las Quinasa Fosfoinosítidos-3 , Ingeniería de Proteínas , Receptores de IgG/genética , Receptores de IgG/metabolismo , Receptores de Interferón/metabolismo , Factor de Transcripción STAT1/genética , Transducción de Señal/efectos de los fármacos , Transducción de Señal/genética , Activación Transcripcional/efectos de los fármacos , Receptor de Interferón gammaRESUMEN
Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin.
Asunto(s)
Venenos de Abeja/enzimología , Inmunidad Innata/inmunología , Inmunoglobulina E/biosíntesis , Proteínas de Insectos/inmunología , Lisofosfolípidos/inmunología , Fosfolipasas A2/inmunología , Receptores de Interleucina/inmunología , Células Th2/inmunología , Anafilaxia/etiología , Anafilaxia/inmunología , Anafilaxia/prevención & control , Animales , Venenos de Abeja/toxicidad , Venenos de Crotálidos/inmunología , Genes Reporteros , Inmunoglobulina E/inmunología , Inmunoglobulina G/biosíntesis , Inmunoglobulina G/inmunología , Proteína 1 Similar al Receptor de Interleucina-1 , Interleucina-33 , Interleucina-4/biosíntesis , Interleucina-4/genética , Interleucinas/inmunología , Activación de Linfocitos , Meliteno/inmunología , Lípidos de la Membrana/metabolismo , Ratones , Ratones Endogámicos BALB C , Ratones Noqueados , Factor 88 de Diferenciación Mieloide/deficiencia , Factor 88 de Diferenciación Mieloide/fisiología , Ovalbúmina/inmunología , Fosfolípidos/metabolismo , Receptores de IgE/inmunologíaRESUMEN
Allergies are generally thought to be a detrimental outcome of a mistargeted immune response that evolved to provide immunity to macroparasites. Here we present arguments to suggest that allergic immunity has an important role in host defence against noxious environmental substances, including venoms, haematophagous fluids, environmental xenobiotics and irritants. We argue that appropriately targeted allergic reactions are beneficial, although they can become detrimental when excessive. Furthermore, we suggest that allergic hypersensitivity evolved to elicit anticipatory responses and to promote avoidance of suboptimal environments.