Negligible adsorption and toxicity of microplastic fibers in disinfected secondary effluents.

Wastewater treatment plants play a crucial role in controlling the transport of pollutants to the environment and often discharge persistent contaminants such as synthetic microplastic fibers (MFs) to the ecosystem. In this study, we examined the fate and toxicity of polyethylene terephthalate (PET) MFs fabricated from commercial cloth in post-disinfection secondary effluents by employing conditions that closely mimic disinfection processes applied in wastewater treatment plants. Challenging conventional assumptions, this study illustrated that oxidative treatment by chlorination and ozonation incurred no significant modification to the surface morphology of the MFs. Additionally, experimental results demonstrated that both pristine and oxidized MFs have minimal adsorption potential towards contaminants of emerging concern in both effluents and alkaline water. The limited adsorption was attributed to the inert nature of MFs and low surface area to volume ratio. Slight adsorption was observed for sotalol, sulfamethoxazole, and thiabendazole in alkaline water, where the governing adsorption interactions were suggested to be hydrogen bonding and electrostatic forces. Acute exposure experiments on human cells revealed no immediate toxicity; however, the chronic and long-term consequences of the exposure should be further investigated. Overall, despite the concern associated with MFs pollution, this work demonstrates the overall indifference of MFs in WWTP (i.e., minor effects of disinfection on MFs surface properties and limited adsorption potential toward a mix of trace organic pollutants), which does not change their acute toxicity toward living forms.

Risk assessment framework for microplastic in marine environments.

Constantly raising microplastic (MP) contamination of water sources poses a direct threat to the gentle balance of the marine environment. This study focuses on a multifactor hazard evaluation of conventional (polyethylene - PE, polypropylene - PP, and polystyrene - PS) and alternative (polyethylene terephthalate with 25 % or 50 % recycled material and polylactic acid) plastics. The risk assessment framework explored included MP abundance, water acidification potential, surface oxidation, fragmentation, and bacterial growth inhibition. Based on MP monitoring campaigns worldwide, we conclude that PE-based plastics are the most abundant MPs in water samples (comprise up to 82 % the MP in those samples). A year-long weathering experiment showed that PS-based and PP-based plastics were oxidized to a higher extent, resulting in the highest water acidification with pH reduction of up to three orders of magnitude. Finally, our laboratory experiments showed that weathered PS was the most fragile plastic during mechanical degradation, while both PP- and PS-based plastic extracts showed a significant growth inhibition toward the marine microorganisms (Bacillus sp. and Pseudoaltermonas sp). Using the examined factors as weighted inputs into our framework, this holistic evaluation of hazards suggest that PP-based plastic products were the most hazardous compared to the other conventional and alternative plastic types.

Mediterranean microplastic contamination: Israel's coastline contributions.

This study provides an analysis of the current state of microplastic (MP) contamination along the Mediterranean coastline of Israel. Six strategic sites were monitored in this study - each representing a unique coastal environment. We conclude that Tel Aviv and Hadera, both located near stream estuaries, were highly contaminated (18,777 particles/m3) with MP compared to the other locations. The MP detected included both secondary MP and pristine polymeric pellets. In-depth characterization of the MP illustrated a large percentage of both fragmented and film MP morphologies and the most common MP polymers were polyethylene and polypropylene. Further particle analysis showed that MPs were contaminated with biofilm, including microorganisms such as diatoms, as well as metal residues. Through the spatial analysis presented herein we suggest that local rivers are significant contributors to MP contamination along the Mediterranean Sea coastline of Israel and may pose a direct threat to environment and human health.

Slow release of copper from jellyfish-based hydrogels for soil enrichment.

Nanotechnology has shown great potential to increase global food production and enhance food security. However, large-scale application of nano-enabled plant agriculture necessitates careful adjustments in design to overcome barriers associated with targeted nanomaterial delivery and their safety concerns. The research herein proposes the delivery of copper (Cu) from immobilized and non-immobilized copper oxide nanoparticles (Cu2O), an active nanomaterial with antifungal and micro-nutrient properties. A benign and biodegradable jellyfish-based hydrogel was used as a platform during Cu2O delivery to soils. The delivery kinetics and Cu dissolution from the nanocomposite were compared to those obtained with crosslinked ionic Cu in hydrogel, which was found to be a less controlled composite. In addition, changing environmental conditions from DI to soil extracts resulted in a decrease in the Cu dissolution rate (from 0.025 to 0.015 h-1) and an increase in the overall normalized Cu release (0.27 to 0.76 mg g-1). Use of hydrogels from natural sources allowed biodegradability over several months, adding nutrients (in the form of elements such as sulfur, nitrogen, and carbon) back to the environment, which ultimately minimizes nanomaterial required for a given desired nanomaterial yield and enhances the overall performance. Altogether, this work demonstrates the potential of Cu2O embedded hydrogels as a benign composite for Cu slow-release and therefore bolsters the field of nano-enabled plant agriculture and supports its safe deployment at large scales.

Interactions of microplastics and organic compounds in aquatic environments: A case study of augmented joint toxicity.

High levels of persistent contaminants such as microplastics (MPs) and trace organic compounds (TrOCs) in the aquatic environment have become a major threat on the ecosystem and human health. While MP's role as a vector of environmental TrOCs is widely discussed in the literature, the corresponding implications of the interaction between these two compounds on human health (i.e., their joint toxic effect) have not been illustrated. Using a TrOCs model (Triclosan, TCS) and primary MPs (polystyrene microbeads), this work evaluates the sorption and desorption potential of TCS and MPs in simulated environmental and cellular conditions, respectively, and estimates the single and joint toxicity of these interactions toward human cells (Caco-2). Surface functionality of the microbeads highly increased their adsorption capacity of TCS, from 2.3 mg TCS for non-functionalized microbeads to 4.6 mg and 6.1 mg TCS per gram of microbeads for amino- and carboxyl-functionalized MPs, respectively. Using non-functionalized MPs, non-specific "hydrophobic-like" interactions and π-π interactions dominated the sorption mechanism of TCS; however, the addition of hydrogen interactions between functionalized microbeads and TCS increased the microbeads' overall sorption capacity. TCS was desorbed from both functionalized and non-functionalized MPs when changing from environmental conditions to cellular conditions. Desorption was found to be dependent on the matrix complexity and protein content as well as microbead functionality. Finally, toxicity tests suggested that while low concentrations of TCS and MPs (separately) have minor toxic effect toward Caco-2 cells, TCS-sorbed MPs at similar concentrations have an order of magnitude higher toxicity than pristine MPs, potentially associated with the close interaction of both MP and TCS with the cells. Overall, this study not only elucidates the role of MPs as a TrOC vector, but also demonstrates a realistic scenario in which co-presence of these environmental contaminants poses risks to the environment and human health.

Engineered Polystyrene-Based Microplastics of High Environmental Relevance.

Microplastic (MP) pollution-an emerging environmental challenge of the 21st century-refers to accumulation of environmentally weathered polymer-based particles with potential environmental and health risks. Because of technical and practical challenges when using environmental MPs for risk assessment, most available data are generated using plastic models of limited environmental relevancy (i.e., with physicochemical characteristics inherently different from those of environmental MPs). In this study, we assess the effect of dominant weathering conditions-including thermal, photo-, and mechanical degradation-on surface and bulk characteristics of polystyrene (PS)-based single-use products. Further, we augment the environmental relevance of model-enabled risk assessment through the design of engineered MPs. A set of optimized laboratory-based weathering conditions demonstrated a synergetic effect on the PS-based plastic, which was fragmented into millions of 1-3 µm MP particles in under 16 h. The physicochemical properties of these engineered MPs were compared to those of their environmental counterpart and PS microbeads often used as MP models. The engineered MPs exhibit high environmental relevance with rough and oxidized surfaces and a heterogeneous fragmented morphology. Our results suggest that this top-down synthesis protocol combining major weathering mechanisms can fabricate improved, realistic, and reproducible PS-based plastic models with high levels of control over the particles' properties. Through increased environmental relevancy, our plastic model bolsters the field of risk assessment, enabling more reliable estimations of risk associated with an emerging pollutant of global concern.

Questioning the suitability of available microplastics models for risk assessment - A critical review.

The rise of microplastic (MP) pollution in the environment has been bolstering concerns regarding MPs' unknown environmental fate, transport, and potential toxicity toward living forms. However, the use of real environmental plastics for risk assessment is often hindered due to technical and practical challenges such as plastics' heterogeneity and their wide size distribution in the environment. To overcome this issue, most available data in the field is generated using plastic models as surrogates for environmental samples. In this critical review, we describe the gaps in risk assessments drawn from these plastic models. Specifically, we compare physicochemical properties of real environmental plastic particles to synthesized polymeric micro-beads, one of the most commonly used plastic models in current literature. Several surface and bulk characteristics including size, surface chemistry, polymer type, and morphology are shown to not only be inherently different between environmental MP's and synthesized micro-beads, but also drive behavior in fate, transport, and toxicity assays. We highlight the importance of expressing real-world physicochemical characteristics in representative MP models and outline how current state-of-the-art models are limited in this regard. To address this issue, we suggest future areas of research such as combinations of mechanical, photochemical, and thermal degradation processes to simulate real-world weathering, all in an effort to increase realism of plastic modeling and allow more robust and reliable environmental MP risk assessment in the future.

High-Voltage, Pulsed Electric Fields Eliminate Pseudomonas aeruginosa Stable Infection in a Mouse Burn Model.

Objective: The incidence of severe infectious complications after burn injury increases mortality by 40%. However, traditional approaches for managing burn infections are not always effective. High-voltage, pulsed electric field (PEF) treatment shortly after a burn injury has demonstrated an antimicrobial effect in vivo; however, the working parameters and long-term effects of PEF treatment have not yet been investigated. Approach: Nine sets of PEF parameters were investigated to optimize the applied voltage, pulse duration, and frequency or pulse repetition for disinfection of Pseudomonas aeruginosa infection in a stable mouse burn wound model. The bacterial load after PEF administration was monitored for 3 days through bioluminescence imaging. Histological assessments and inflammation response analyses were performed at 1 and 24 h after the therapy. Results: Among all tested PEF parameters, the best disinfection efficacy of P. aeruginosa infection was achieved with a combination of 500 V, 100 µs, and 200 pulses delivered at 3 Hz through two plate electrodes positioned 1 mm apart for up to 3 days after the injury. Histological examinations revealed fewer inflammatory signs in PEF-treated wounds compared with untreated infected burns. Moreover, the expression levels of multiple inflammatory-related cytokines (interleukin [IL]-1α/ß, IL-6, IL-10, leukemia inhibitory factor [LIF], and tumor necrosis factor-alpha [TNF-α]), chemokines (macrophage inflammatory protein [MIP]-1α/ß and monocyte chemoattractant protein-1 [MCP-1]), and inflammation-related factors (vascular endothelial growth factor [VEGF], macrophage colony-stimulating factor [M-CSF], and granulocyte-macrophage colony-stimulating factor [G-CSF]) were significantly decreased in the infected burn wound after PEF treatment. Innovation: We showed that PEF treatment on infected wounds reduces the P. aeruginosa load and modulates inflammatory responses. Conclusion: The data presented in this study suggest that PEF treatment is a potent candidate for antimicrobial therapy for P. aeruginosa burn infections.

Selective Inactivation of Pseudomonas aeruginosa and Staphylococcus epidermidis with Pulsed Electric Fields and Antibiotics.

Objective: Increasing numbers of multidrug-resistant bacteria make many antibiotics ineffective; therefore, new approaches to combat microbial infections are needed. In addition, antibiotics are not selective-they kill pathogenic organisms as well as organisms that could positively contribute to wound healing (bio flora). Approach: Here we report on selective inactivation of Pseudomonas aeruginosa and Staphylococcus epidermidis, potential pathogens involved in wound infections with pulsed electric fields (PEFs) and antibiotics (mix of penicillin, streptomycin, and nystatin). Results: Using a Taguchi experimental design in vitro, we found that, under similar electric field strengths, the pulse duration is the most important parameter for P. aeruginosa inactivation, followed by the number of pulses and pulse frequency. P. aeruginosa, a potential severe pathogen, is more sensitive than the less pathogenic S. epidermidis to PEF (alone or in combination with antibiotics). Applying 200 pulses with a duration of 60 µs at 2.8 Hz, the minimum electric fields of 308.8 ± 28.3 and 378.4 ± 12.9 V/mm were required to inactive P. aeruginosa and S. epidermidis, respectively. Addition of antibiotics reduced the threshold for minimum electric fields required to inactivate the bacteria. Innovation: This study provides essential information, such as critical electric field parameters for bacteria inactivation, required for developing in vivo treatment and clinical protocols for using PEF for wound healing. Conclusion: A combination of PEFs with antibiotics reduces the electric field threshold required for bacteria disinfection. Such an approach simplifies devices required to disinfect large areas of infected wounds.

IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa.

Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm-1, when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.