[The quality control of nutrient medium in laboratory bacteriologic diagnostics of diphtheria].

The article deals with the results of studying the growth characteristics of nine nutrient mediums for primary plating of pathologic material. It is demonstrated that for successful and proper functioning of bacteriologic laboratory providing bacterial analysis for diphtheria the permanent quality control is needed to monitor the nutrient mediums for primary plating. The quality control is applied to evaluate the growth characteristics on such criteria as germination of isolated culture, intensity of its growth in 24 and 48 hours, optimal size of colonies characterized by their cultural characteristics, inhibiting activity concerning concurrent microflora.

[Characteristics of Bordetelia pertussis strains isolated from pertussis patients in Moscow by using multilocus sequencing].

AIM: Genotyping of B. pertussis strains isolated from pertussis patients in Moscow. MATERIALS AND METHODS: 53 strains of B. pertussis isolated from pertussis patients in Moscow in 2007 - 2010 as well as 3 vaccine strains currently used in Russia for the production of DTP vaccine were studied by multilocus sequencing (MLST) based on allele combinations of ptxA, ptxC and tcfA genes. RESULTS: A genetic characteristic of B. pertussis strains isolated from pertussis patients in Moscow by using MLST is presented. Allele profile analysis of the studied B. pertussis strains was performed, 4 sequence types (ST) were identified--ST1, ST2, ST3 and ST5, most of the circulating strains (86.7%) were shown to belong to ST5, equal percentage of cases (5.7%)--to ST2 and ST3, and 1.9%--to ST1, while 2 vaccine production strains belong to ST2 and 1 - to ST1. CONCLUSION: Clonal structure of contemporary Moscow strains was shown to be different from strain structure used for the production of DTP vaccine.