RESUMEN
We report blue- and green-emitting two-photon probes derived from naphthalene and fluorene derivatives (as fluorophores) and an endoplasmic reticulum (ER) retrieval peptide (KDEL; as an ER-targeting moiety) that can detect the ER in a live cell by both one-photon and two-photon microscopy (TPM) and in a live tissue by TPM.
Asunto(s)
Retículo Endoplásmico/química , Colorantes Fluorescentes/química , Microscopía de Fluorescencia por Excitación Multifotónica , Fotones , Fluorenos/química , Células HeLa , Humanos , Estructura Molecular , Naftalenos/química , Imagen Óptica , Péptidos/químicaRESUMEN
Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other.
