Effect of biogenic bismuth nanoparticle on the expression of New Delhi metallo-ß-lactamase (NDM) gene in Multidrug-Resistant Klebsiella pneumoniae.

Aim and background: The emergence of Multidrug-Resistant Klebsiella pneumoniae is a global concern due to high mortality and treatment challenges. One of the most important genes for resistance is NDM, which makes the organism resistant to most antibiotics. Today, the use of nanoparticles as therapeutic options has stimulated researchers around the world to investigate its effects. The aim of this study was to investigate the effects of biosynthesized bismuth nanoparticles (Bi NPs) on the expression of NDM genes in multidrug-resistant K. pneumoniae. Materials and methods: In this study, 5 multidrug-resistant K. pneumoniae clinical isolates from patients referred to Afzalipour Hospital in Kerman, Iran, were used. Antibiotic sensitivity test was performed by disc diffusion method. The presence of the NDM gene was checked in isolates using a PCR reaction. The isolates containing the NDM gene were exposed to the biosynthesized and characterized bismuth nanoparticles, and the effects on the expression of the NDM gene was investigated using real-time PCR. Results: The results showed that 3 isolates of K. pneumoniae had NDM genes. In TEM and SEM analysis showed that the nanoparticles had a spherical structure and an average size of 22.36 nm. The investigation of biogenic Bi NPs on the expression of the NDM gene demonstrated that the samples treated with bismuth nanoparticles decreased the expression of the NDM gene by 1.6 times compared to the control group (p < 0.011). Conclusion: Our findings showed that biosynthesized Bi NPs have a high potential to deal with antibiotic resistance genes and can be a promising for treatment.

The use of phages for the biosynthesis of metal nanoparticles and their biological applications: A review.

Nowadays, the use of biological methods of synthesis of nanoparticles as substitutes for methods that use high energy and consumption of expensive and dangerous materials is of interest to researchers all over the world. Biological methods of synthesising metal nanoparticles are very important because they are easy, affordable, safe, environmentally friendly and able to control the size and shape of nanoparticles. One of the methods that is of interest today is the use of bacteriophages as the most abundant organisms in nature in the synthesis of metal nanoparticles. Nanomaterials biosynthesized from phages have shown various clinical applications, including antimicrobial activities, biomedical sensors, drug and gene delivery systems, cancer treatment and tissue regeneration. Therefore, the purpose of this review is to investigate the biosynthesis of metal nanoparticles with phages and their biomedical applications.

The use of Ephedra herbs in the treatment of COVID-19.

Objective: Ephedra herbs are the only extant genus in its family, Ephedraceae, and order, Ephedrales. It has been prescribed in traditional medicine for improving headaches and respiratory infections. On the other hand, because the coronavirus disease 2019 (COVID-19) causes respiratory problems and COVID-19 pandemic is the most widespread outbreak that has affected humanity in the last century, the current review aims using literature search to investigate the effects of the Ephedra herbs compounds on COVID-19 to supply a reference for its clinical application in the inhibition and remedy of COVID-19. Materials and Methods: This review was performed using articles published in various databases, including Web of Science, PubMed, Scopus, and Google Scholar, without a time limit. For this paper, the following keywords were used: "Ephedra", "coronavirus disease 2019", "COVID-19", "Severe acute respiratory syndrome coronavirus 2" or "SARS CoV 2". Results: The results of this review show that the Ephedra herbs have effectiveness on COVID-19 and its compounds can bind to angiotensin-converting enzyme 2 (ACE2) with a high affinity and act as a blocker and prevent the binding of the virus. Conclusion: Some plants used in traditional medicine, including the Ephedra herbs, with their active compounds, can be considered a candidate with high potential for the control and prevention of COVID-19.

The impact of COVID-19 on liver injury in various age.

The coronavirus disease 2019 (COVID-19) disease was first detected in December 2019 in Wuhan, China. This disease is currently one of the most important global health problems. The novel coronavirus COVID-19 is a respiratory illness, that has caused a deadly pandemic that is spreading rapidly around the world. It is not only a respiratory system virus that causes severe lung disease, but also a systemic disease agent that can affect all systems. People with COVID-19 disease usually have respiratory signs, however, the liver disorder is not an uncommon presentation. In addition, many studies around the world have revealed that the liver is injured to various degrees in patients with severe acute respiratory syndrome coronavirus 2 disease. This review mainly focuses on the impact of COVID-19 on Liver Injury at various ages.

An insight into biofabrication of selenium nanostructures and their biomedical application.

Evidence shows that nanoparticles exert lower toxicity, improved targeting, and enhanced bioactivity, and provide versatile means to control the release profile of the encapsulated moiety. Among different NPs, inorganic nanoparticles (Ag, Au, Ce, Fe, Se, Te, Zn, etc.) possess a considerable place owing to their unique bioactivities in nanoforms. Selenium, an essential trace element, played a vital role in the growth and development of living organisms. It has attracted great interest as a therapeutic factor without significant adverse effects in medicine at recommended dose. Selenium nanoparticles can be fabricated by physical, biological, and chemical approaches. The biosynthesis of nanoparticles is shown an advance compared to other procedures, because it is environmentally friendly, relatively reproducible, easily accessible, biodegradable, and often results in more stable materials. The effect of size, shape, and synthesis methods on their applications in biological systems investigated by several studies. This review focused on the procedures for the synthesis of selenium nanoparticles, in particular the biogenesis of selenium nanoparticles and their biomedical characteristics, such as antibacterial, antiviral, antifungal, and antiparasitic properties. Eventually, a comprehensive future perspective of selenium nanoparticles was also presented.

COVID-19 (Coronavirus Disease 2019): A New Coronavirus Disease.

Coronavirus disease 2019 (COVID-19) is a type of viral pneumonia with an uncommon outbreak in Wuhan, China, in December 2019, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV2). SARS-CoV-2 is extremely contagious and has resulted in a fast pandemic of COVID-19. Currently, COVID-19 is on the rise around the world, and it poses a severe threat to public health around the world. This review provides an overview about the COVID-19 virus to increase public awareness and understanding of the virus and its consequences in terms of history, epidemiology, structure, genome, clinical symptoms, diagnosis, prevention, and treatment.