RESUMEN
Luminescent europium complexes are used in a broad range of applications as a result of their particular emissive properties. The synthesis and application of bright, highly water-soluble, and negatively charged sulfonic- or carboxylic acid derivatives of para-substituted aryl-alkynyl triazacyclononane complexes are described. Introduction of the charged solubilizing moieties suppresses cellular uptake or adsorption to living cells making them applicable for labeling and performing assays on membrane receptors. These europium complexes are applied to monitor fluorescent ligand binding on cell-surface proteins with time-resolved Förster resonance energy transfer (TR-FRET) assays in plate-based format and using TR-FRET microscopy.
Asunto(s)
Compuestos Aza/análisis , Complejos de Coordinación/análisis , Europio/análisis , Transferencia Resonante de Energía de Fluorescencia/métodos , Sustancias Luminiscentes/análisis , Microscopía/métodos , Piperidinas/análisis , Receptores Acoplados a Proteínas G/metabolismo , Compuestos Aza/metabolismo , Complejos de Coordinación/metabolismo , Europio/metabolismo , Células HEK293 , Humanos , Ligandos , Sustancias Luminiscentes/metabolismo , Piperidinas/metabolismo , Unión Proteica , Receptores Acoplados a Proteínas G/análisis , Solubilidad , Agua/químicaRESUMEN
The conjugation of arginine residues at the ends of a metallo-supramolecular triple-helical cylinder enables absolute control over the helicity of the cylinder core, and boosts the DNA junction recognition by the complexes and their activity against a cancer cell line.
