Expression profile of immunoregulatory factors in canine tumors.

Cancers utilize a variety of molecules to escape host immune responses. Better understanding the immune environment surrounding cancer may facilitate application of innovative cancer immunotherapies, such as immune checkpoint inhibitors, to dogs as well as humans. In this study, we screened the expression of 20 immune regulatory molecules in diverse canine tumors (n = 59). Quantitative RT-PCR (qPCR) analysis revealed that some immune regulatory molecules, such as LGALS9 (coding Galectin-9) and CD48, were expressed in most canine tumors, but other molecules, such as CD274 (coding PD-L1), IL4I1, PVR, TNFSF18, ICOSLG, and TNFSF4, were rarely expressed. NECTIN2 was highly expressed in epithelial tumors but was low in non-epithelial tumors. In contrast, VSIR and CD200 expressions were low in epithelial tumors but high in non-epithelial tumors. Interestingly, several tumors expressed distinctive immunoregulatory factors. Hepatocellular carcinomas expressed FGL1, mast cell tumors expressed PDCD1LG2 (coding PD-L2), transitional cell carcinomas expressed VTCN1 (coding B7x), and lymphomas and squamous cell carcinomas expressed CD70. Consistent with qPCR results, immunofluorescence staining confirmed that hepatocellular carcinomas expressed FGL-1 protein. Thus, this study reveals the expression profile of immunoregulatory molecules in canine tumors and opens the door to better understanding the relationship between canine tumors and host immunity.

Modified Toyoda technique for total cystectomy and cutaneous ureterostomy in a cat.

OBJECTIVES: To report the technique and outcome of a cat treated with a total cystectomy and bilateral cutaneous ureterostomy. ANIMALS: A 16-year-old male castrated domestic shorthair cat. STUDY DESIGN: Case report. CASE PRESENTATION: A cat was referred after a 2-week history of pollakiuria and hematuria. Transitional cell carcinoma (TCC) was suspected based on ultrasonographic, cytologic, and contrast computed tomographic (CT) findings. A total cystectomy was combined with a modified cutaneous ureterostomy: the ureter, incised like a fish-mouth aperture, was anastomosed to the skin after the creation of a rectangular-shaped defect. Complete excision of a TCC was confirmed histologically. RESULTS: Ureteral stents were removed 7 days (left) and 28 days (right) postoperatively. The cat's incontinence was managed with an absorbent diaper surrounding the ureteral stomata. The right ureter became obstructed 14 months after surgery, and the cat died at home approximately 16 months after surgery. In spite of the urinary incontinence, the owner was satisfied with the surgery and evaluated the cat's quality of life as satisfactory. CONCLUSIONS: The total cystectomy and cutaneous ureterostomy described here allowed urinary excretion and resulted in long-term survival of a cat with bladder TCC.

Assessment of changes in blood pancreatic lipase activities using FDC-v-LIP in dogs that underwent various surgical procedures.

OBJECTIVE: To describe the perioperative changes in blood pancreatic lipase activity and explore the contributing clinical factors associated with these changes. DESIGN: Prospective observational study. SETTING: University teaching hospital. ANIMALS: One hundred and four dogs underwent various surgical procedures under general anesthesia. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Blood pancreatic lipase activities, which were measured using FUJI DRI-CHEM v-Lip-P (FDC-v-Lip), significantly increased postoperatively compared to preoperative measurements (premedian 58.5 U/L [range, 23-157] vs. postmedian 80 U/L [range, 22-1000], P < 0.0001). The patient with a postoperative increase in FDC-v-Lip over the normal range (35 dogs [33.6%]) had significantly higher preoperative FDC-v-Lip values. CONCLUSIONS: In this study, dogs had significantly increased pancreas-specific lipase activities after surgical procedures under general anesthesia. Direct contributors to the increase and its relevance to clinical and histological pancreatitis should be determined in the future.

Local Hemodynamic Changes Immediately after Correction of an Aberrant Right Subclavian Artery in a Dog: A Contrast Computed Tomographic Study.

A 1-year-old female Akita dog was referred for intermittent regurgitation. Computed tomographic angiography (CTA) showed an aberrant right subclavian artery (ARSA), resulting in constriction of the esophagus. After surgical ligation of the ARSA, CTA showed that the ARSA was not enhanced by contrast medium, and that sufficient collateral circulation of the right forelimb was supplied through the vertebral artery. Furthermore, the right and left vertebral arteries merged into the basilar artery at the level of the atlas, and no abnormal expansion of the ventral spinal artery was observed. Overall, we demonstrated the importance of post-surgical CTA for identification of surgical complications, including the formation of abnormal vessel alterations.