Mortality associated with acute respiratory distress syndrome, 2009-2019: a systematic review and meta-analysis.

Background: Acute respiratory distress syndrome (ARDS) occurs commonly in intensive care units. The reported mortality rates in studies evaluating ARDS are highly variable. Objective: To investigate mortality rates due to ARDS from before the 2009 H1N1 influenza pandemic began until the start of coronavirus disease 2019 (COVID-19) pandemic. Design: We performed a systematic search and then ran a proportional meta-analysis for mortality. We ran our analysis in three ways: for randomised controlled trials only, for observational studies only, and for randomised controlled trials and observational studies combined. Data sources: MEDLINE and Embase, using a highly sensitive criterion and limiting the search to studies published from January 2009 to December 2019. Review methods: Two of us independently screened titles and abstracts to first identify studies and then complete full text reviews of selected studies. We assessed risk of bias using the Cochrane RoB-2 (a risk-of-bias tool for randomised trials) and the Cochrane ROBINS-1 (a risk-of-bias tool for non-randomised studies of interventions). Results: We screened 5844 citations, of which 102 fully met our inclusion criteria. These included 34 randomised controlled trials and 68 observational studies, with a total of 24 158 patients. The weighted pooled mortality rate for all 102 studies published from 2009 to 2019 was 39.4% (95% CI, 37.0-41.8%). Mortality was higher in observational studies compared with randomised controlled trials (41.8% [95% CI, 38.9-44.8%] v 34.5% [95% CI, 30.6-38.5%]; P = 0.005). Conclusions: Over the past decade, mortality rates due to ARDS were high. There is a clear distinction between mortality in observational studies and in randomised controlled trials. Future studies need to report mortality for different ARDS phenotypes and closely adhere to evidence-based medicine. PROSPERO registration: CRD42020149712 (April 2020).

Effect of potassium fertilizer on the growth, physiological parameters, and water status of Brassica juncea cultivars under different irrigation regimes.

Abiotic stress, especially a lack of water, can significantly reduce crop yields. In this study, we evaluated the physiological and biochemical effects of potassium sulfate (K2SO4) fertilizer and varied irrigation regimes on the economically significant oilseed crop, Brassica juncea L, under open field conditions. Two cultivars (RH-725 and RH-749) of B. juncea were used in a randomized complete block design experiment with three replicates. Irrigation regimes consisted of a control (double irrigation: once at the 50% flowering and another at 50% fruiting stages), early irrigation (at 50% flowering only), late irrigation (at 50% fruiting only) and stress (no irrigation). The K2SO4 applications were: control (K0, no fertilization); K1, 10 kg ha-1; and K2, 20 kg ha-1. We measured growth via fresh and dry plant weight, plant height, root length, and leaf area. All the growth parameters were higher in RH-749. The physiological attributes, including the membrane stability index and relative water content, were higher at the 50% flowering stage in RH-749. The amount of antioxidant enzymes (catalase (CAT), guaiacol peroxidase (POX), ascorbate peroxidase (APX), and superoxide dismutase (SOD)) was enhanced when both plants were fertilized during water stress. All of these enzymes had higher activity in RH-749. The total chlorophyll content and photosynthesis rate were considerably higher in RH-749, which leaked fewer electrolytes and maintained a less destructive osmotic potential under limited water conditions. The results indicated that it is water-stress tolerant when given a high concentration of K2SO4, which alleviated the adverse effects of water stress on growth and physiology.

Mitigation of saline conditions in watermelon with mycorrhiza and silicon application.

Citrullus lanatus L. is critical vegetable for salinity stress. Arbuscular mycorrhizal fungi (AMF) and silicon treatments are known to help as bio-ameliorator of saline soils that can improve salinity tolerance in plants. But their combined effect has never been examined on watermelon therefore, present study investigated the effect of inoculation with the Arbuscular mycorrhizal fungi (AMF) along with silicon on the growth and yield parameters, antioxidant enzyme activities, pigment and mineral content of Citrullus lanatus L. plants grown during salt stress conditions. Outcomes from the study point out that salt stressed watermelon plants showed the best morphological and biochemical values when inoculated with Silicon (4 mM) + Glomus mosseae + Gigaspora gigantean. In addition, the plants inoculated by similar treatment demonstrated less osmotic activity, electrolyte leakage, as well as peroxide content. Treatments comprising Silicon (4 mM) with either Glomus mosseae and Gigaspora gigantean also performed significantly similar for most of the traits studied in the present investigation and better than the treatment only with either one of Glomus mosseae and Gigaspora gigantean. Antioxidant efficiency of melon was certainly appreciably enhanced after incubation with AMF and Si combination in salinity stress. Overall, the application of mycorrhiza and silicon can be considered to overcome the salinity stress in watermelon.

Mycorrhizal fungi and Pseudomonas fluorescens application reduces root-knot nematode (Meloidogyne javanica) infestation in eggplant.

Eggplant cultivation is subjected to attacks by numbers of pests and diseases from the nursery stage until harvest. Root-knot nematode (M. javanica) is one of the most significant restrictions in the successful cultivation of eggplant as it damages the crop year-round. One of the most essential classes of plant symbionts is arbuscular mycorrhizal fungi (AMF) and phosphate solubilizing bacteria (PSB), which significantly impact plant development, feeding, disease tolerance, and resistance to M. javanica. Eggplant seedlings were inoculated with two mycorrhizal fungi, Glomus mosseae (Gm) and Gigaspora gigantea (Gg), together with the phosphate-solubilizing bacteria (PSB) Pseudomonas fluorescens (Pf; ATCC-17400) under the presence of nematodes inoculation of Meloidogyne javanica as 1000 eggs of M. javanica in each pot. Observations were recorded for 9 morphological traits, 6 fruit morphometric traits using Tomato Analyzer (version 4) software program, and 4 fruit biochemical traits. Along with the data recorded for mycorrhization (%), number of galls and reaction to RKN. Plants inoculated with the consortium (Pf + Gm + Gg) performed substantially better for most traits. Furthermore, the eggplant plants treated with consortium developed the highest levels of fruit biochemical content along with the highest level of mycorrhization (68.20%). Except for certain fruit morphometric traits, the treatment containing Pf + Gg outperformed the treatment containing Pf + Gm. Overall, this research showed that AM fungi could be a sustainable solution to the eggplant RKN problem.

Effect of the diverse combinations of useful microbes and chemical fertilizers on important traits of potato.

The belowground soil environment is an active space for microbes, particularly Arbuscular Mycorrhizal Fungi (AMF) and P hosphate Solubilizing Bacteria (PSB) that can colonize with roots of higher plants. In the present experiment, we evaluated the combination of microbial inoculants with the different doses of urea and superphosphate in a complete randomized block design (CRBD). Three different doses of urea and superphosphate were tested, i.e., recommended dose, 75% of the recommended dose and 125% of the recommended dose, independently and in combination with three microbial groups viz. Glomus mosseae (AMF), Bacillus subtilis (PSB) and Nitrifying microorganisms (Nitrosomonas + Nitrobacter, NN). Overall, there were 16 treatment combinations used, and studied the number of tubers per plant, the weight of tubers, moisture content, and the number of nodes per tubers which were best in treatment comprising of AMF + PSB + NN + 75% of urea + superphosphate. From our results, it is suggested for the growers to use a lesser quantity of fertilizers from the recommended dose along with some bioinoculants to maintain the soil fertility and also to achieve the yield targets by decreasing the cost of chemical fertilizers.

Effects of arbuscular mycorrhizal fungi and P-solubilizing Pseudomonas fluorescence (ATCC-17400) on morphological traits and mineral content of sesame.

Sesame (Sesamum indicum L.) is an important staple crop of the family Pedaliaceae. The commercial production of sesame is still dependent on the applications of chemical fertilizers. Mycorrhiza inoculum resulted in better morphological and biochemical traits in vegetables. Thus, here the outcome of arbuscular mycorrhizal fungi (AMF) and Pseudomonas fluorescence (ATCC-17400) inoculation was studied in the pot culture experiment. Primarily, there seems to be a promising opportunity of AMF in sesame under pot and field trials because of enhanced morphological parameters, especially root weight, and disparities in nutrients and metabolites. The AMF appears to be an option to boost plant growth, mineral content, and sesame yield. The AMF treatment with Pseudomonas fluorescence strain (ATCC-17400) determined the maximum values for the morphological traits and mineral content. Overall, our study highlights mycorrhizal fungi and other microbes efficacy in achieving a successful sesame production.

Acacia from a Breeding and Biotechnological Perspective: A Review.

Members of genus Acacia comprises of trees used as fuelwood, timber and fodder. Moreover, some parts of plants are also used for their therapeutic properties. The development and applications of breeding and biotechnological tools are advancing at a significantly fast rate. Molecular markers and genomics offer vital information with regard to the inherited variation. The aim of this study to complied and discussed the developments in molecular maker technology, genomics and genetic engineering concerning genus Acacia. Overall, this information will be useful to gain awareness about the crucial trees in the genus Acacia from breeding and a biotechnological perspective.

Significance of Arbuscular Mycorrhizal Fungi for Acacia: A Review.

Microbes play a vital role in ecosystem stability. Here, microbes-Acacia association is discussed with particular reference to Arbuscular Mycorrhizal Fungi (AMF) which help in the establishment of crop-plants, especially in arid and semi-arid areas. The association helps to restore the structural composition of soil from the hazardous impact of agrochemicals, increase resistance against various pathogenic attack as well as several abiotic stresses. Further, a comparative account of microbes found in the rhizosphere of Acacia is illustrated. Among these, Rhizobia, Acetobacter, Bradyrhizobium, Bacillus, Pseudomonas and Trichoderma were described in detail. All these microbes can be regarded as Plant Growth Promoting Rhizospheric Microbes (PGPM), some of PGPM are Phosphate Solubilizing Microbe (PSM). Both of them help AMF for infecting mycorrhizal hyphae inside the plant cell. Overall, microbes can be used as biofertilizers along with other organic compounds, that can compensate for the nutrient's availability.