The influence of potassium nanoparticles as a foliar fertilizer on onion growth, production, chemical content, and DNA fingerprint.

Potassium is an important macro-fertilizer for plant growth but can be lost from the soil after application via irrigation. Slow-release nano-fertilizers can achieve sustainable crop cultivation and production, so this study evaluated the influence of potassium nanoparticles (K-NPs) with various concentrations (0, 50, 100, and 200 mg/l) on onion development, production, pigments, chemical content, and DNA fingerprint during two sequential agriculture seasons in 2021 and 2022 at a private farm in Zagazig, Sharkia Governorate, Egypt. Spraying onion plants with K-NPs (200 mg/l) significantly improved the vegetative characteristics of onion plant growth and production, as well as increasing the plant pigments and the content of carbohydrate, oil, total indole, and phosphorus in onion bulbs. Similarly, 50 mg/l of K-NPs considerably increased the content of nitrogen, potassium, protein, antioxidant activity, and phenols in the onion bulbs. The content of total flavonoids and anthocyanin was increased with 100 mg/l of K-NPs. In conclusion, the foliar application of K-NPs improves the onion plant yield and quality and can achieve agricultural sustainability.

Effect of foliar application of phosphorus nanoparticles on the performance and sustainable agriculture of sweet corn.

Traditional phosphorus fertilizers are necessary for plant growth but about 80-90% are lost into the surrounding environment via irrigation, therefore nano-fertilizers have been developed as slow-release fertilizers to achieve sustainable agriculture. This trial investigated the impact of the foliar application of hydroxyapatite nanoparticles (HA-NPs) as a source of nano-phosphorus (P-NPs) on two cultivars of sweet corn (yellow and white) throughout two seasons. The morphology and structure of the prepared HA-NPs were characterized via transmission electron microscopy (TEM) and X-ray diffractometry (XRD). In addition, agro-morphological criteria, chemical contents (i.e., photosynthetic pigments, phenols, indoles, minerals, etc.), and genomic template stability percentage (GTS%) were evaluated in the produced sweet corn. The application of 50 mg/l HA-NPs improved the growth characteristics, yield per hectare, leaf pigments, and chemical content of yellow sweet corn, whereas the application of 100 mg/l of HA-NPs to white sweet corn enhanced the vegetative characteristics, production, photosynthetic pigments, phenols, and indoles. The difference in results may be due to the presence of a +ve unique band with SCoT-4 and SCot-2 primers at 1250 and 470 bp in yellow and white corn treated with 50 and 100 mg/l, respectively. The minimum GTS% was recorded at a concentration of 75 mg/l for both white and yellow corn. The HA-NPs can be applied as a foliar source of P-NPs to achieve agricultural sustainability.

The impact of nanofertilizer on agro-morphological criteria, yield, and genomic stability of common bean (Phaseolus vulgaris L.).

The use of agricultural fertilizers is one of the methods to beat the desired enormous increase in universal food production. The application of nanotechnology in agriculture is regarded as one of the promising approaches to elevate crop production. Whereas mineral nutrients play a crucial role in the growth and yield of the common bean. The experiments were conducted to investigate the application effect of micronutrients as nanoparticles (MN-NPs) on the common ben plants. The trial was performed in the field in El-Menofya, Egypt, through two seasons (2019 & 2020) in a randomized complete block design with three replicates and four combinations of MN-NPs (ZnO, MnO2 and MoO3) with concentrations 0, 10, 20, 30, 40 mg/L as a foliar application. The data exhibited that the foliar application of MN-NPs significantly upgraded the vegetative growth characters, flower number/plant, photosynthetic pigments, and yield. The concentration of 40 mg/L of MN-NPs leads to improving the vegetative growth, flowering number, and yield characteristics of the common bean. While the biochemical components varied in their response to MN-NPs combinations. The recommended MN-NPs concentration to ameliorate the common bean growth and yield was 40 mg/L.

The effect of exposure to MoO3-NP and common bean fertilized by MoO3-NPs on biochemical, hematological, and histopathological parameters in rats.

Nanotechnologies has been used to introduce several beneficial tools in the agricultural field. Herein, the effect of molybdenum oxide nanoparticles (MoO3-NPs) was investigated by evaluating the hematological, biochemical, and histopathological parameters in rats orally exposed to MoO3-NPs or fed common beans (CB) fertilized by MoO3-NPs. In the first study, 18 rats were randomly divided into 3 groups: G1 (control group) was given water orally, while G2 and G3 were administered 10 and 40 ppm MoO3-NPs by oral gavage tube, respectively. There was a significant increase in the levels of alanine aminotransferase (ALT), albumin, and total protein; however, there was a a significant decrease in body weight change (BWC), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine, creatine kinase-MB (CK-MB), thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), and testosterone levels in G3 compared to G1. In the second study, 24 rats were divided into 4 groups: the control (C) group was fed a balanced diet, and three groups were fed on a balanced diet plus 10% CB that was fertilized with 0, 10, and 40 ppm MoO3-NPs, resulting in nCB, CB10, and CB40 groups, respectively. This revealed a significant increase in BWC and total food intake (TFI) but a significant decrease in relative kidney weight in all the CB groups compared to the control group. In CB10 and CB40 groups ALT, LDH, TSH, FT3, and testosterone levels were significantly lower than the respective levels in the control group. We concluded that high doses of MoO3-NPs caused more side effects than low doses in both experiments.

Applications of nanotechnology on vegetable crops.

Agriculture is the backbone of most developing countries, and most of their people depend on it for their livelihood. The world population is increased by approximately 83 million people each year, so there is a need to increase agricultural productivity. At present, productivity growth can be achieved either by expanding the area cultivated or increasing crop yields through improving the efficiency of fertilizers used. Therefore, there has been a trend to use modern technologies, such as nanotechnology (NT), to increase the productivity of plants. Where, it is involved in the food production process, from planting to packaging. NT improves plants' ability to absorb nutrients, and the agronomic properties of soil, which improves plant growth and productivity. Economically, NT increased the efficiency of nano-fertilizers, and so contributed to increasing productivity and the production of crops. However, the study of the effect of nanotechnology on the environment of soils and plants did not receive the required study. In this review, a comprehensive survey is exhibited on NT as an effective method in dealing with the problem of fertilizer loss during irrigation. This review discusses the technologies and applications of the latest research findings in this field. Furthermore, this review deals with the forms and types of nanoparticles and the methods of their transmission in plants, as well as their effect on plants (physiological and DNA) as well as on those who eat those plants.

Template-free microwave-assisted hydrothermal synthesis of manganese zinc ferrite as a nanofertilizer for squash plant (Cucurbita pepo L).

Manganese, zinc, and iron are the most essential micronutrients required for plant growth and applied as foliar fertilizers. Herein, a simple template-free microwave-assisted hydrothermal green synthesis technique was adapted to produce manganese zinc ferrite nanoparticles (Mn0.5Zn0.5Fe2O4 NPs) at different temperatures (100, 120, 140, 160 and 180 °C). The prepared nanomaterials were employed at different concentrations (0, 10, 20, and 30 ppm) as foliar nanofertilizers during the squash (Cucurbita pepo L) planting process. X-ray diffraction patterns of the prepared nanomaterials confirmed successful production of the nanoferrite material. The prepared nanofertilizers showed type IV adsorption isotherm characteristic for mesoporous materials. FE-SEM and HR-TEM imaging showed that the nanoparticles were cubic shaped and increased in particle size with the increase in microwave temperature during production. The impact of application of the synthesized ferrite nanoparticles on vegetative growth, proximate analysis, minerals content and the yield of squash plant was investigated for two consecutive successful planting seasons. The nanoferrite synthesized at 160 °C and applied to the growing plants at a concentration of 10 ppm gave the highest increase in % yield (49.3 and 52.9%) compared to the untreated squash for the two consecutive seasons, whereas the maximum organic matter content (73.0 and 72.5%) and total energy (260 and 258.3 kcal/g) in squash leaves were obtained in plants treated with 30 ppm ferrite nanoparticles synthesized at 180 °C. On the other hand, the maximum organic matter content (76.6 and 76.3%) and total energy (253.6 and 250.3 kcal/g) in squash fruits were attained with plants supplied by 20 ppm ferrite nanoparticles synthesized at 160 °C. These results indicate that the simple template-free microwave-assisted hydrothermal green synthesis technique for the production of manganese zinc ferrite nanoparticles yields nanoparticles appropriate for use as fertilizer for Cucurbita pepo L.

The influence of MoO3-NPs on agro-morphological criteria, genomic stability of DNA, biochemical assay, and production of common dry bean (Phaseolus vulgaris L.).

Molybdenum is considered one of the most important micronutrients applied as a foliar fertilizer for common dry bean. In this study, molybdenum oxide nanoparticles (MoO3-NPs) were applied in different concentrations (0, 10, 20, 30 and 40 ppm) over two sequent seasons, 2018 and 2019, to investigate their effect on the plant morphological criteria, yield, and the genomic stability of DNA. The results showed that the application of 40 ppm MoO3-NPs as a foliar fertilizer showed preferable values of plant morphological criteria, such as the number of leaves and branches per plant, as well as the fresh and dry weight with regard to the common bean plant. In addition, the seed yield increased by 82.4% and 84.1% with 40 ppm, while the shoot residue increased by 32.2% and 32.1% with 20 ppm of MoO3-NPs during two seasons, 2018 and 2019, respectively. Furthermore, the common bean treated with 20 and 40 ppm MoO3-NPs had positive unique bands with ISSR primer 848 at 1400 bp (Rf 0.519) and with primer ISSR2M at 200 bp (Rf 0.729), respectively. In addition, SDS-PAGE reveald some proteins in seedlings which were absent in the flowering stage at 154, 102, 64, 37 and 34 KDa, which may be due to differences in plant proteins required for metabolic processes in each stage. In conclusion, the application of 40 ppm MoO3-NPs was more effective on the productivity of the common bean plants.

The effect of white kidney bean fertilized with nano-zinc on nutritional and biochemical aspects in rats.

This study aims to estimate the safety of white kidney bean (WKB) fertilized by zinc oxide nanoparticles (ZnO-NPs) via studying changes of liver and kidney function, lipid profile and histological examination for the liver and kidney tissue in rats fed on it. Twenty Four male albino rats were used in this study divided into four groups; the first fed balanced diet (control group), the second fed WKB treated with normal ZnO (nWKB), the third fed WKB treated with 20 ppm ZnO-NPs (tWKB-1), and the fourth fed WKB treated with 40 ppm ZnO-NPs (tWKB-2). The results revealed that WKB treated with ZnO-NPs reduced body weight, food efficiency ratio, relative liver weight, and relative spleen weight were increased as well as the most biochemical parameters exhibited non-significant changes as compared to control group. Meanwhile, tWKB-2 group demonstrated a decrease in alkaline phosphatase and aspartate transaminase activities as compared to nWKB group.

Preparation and characterization of chitosan/polyacrylic acid/copper nanocomposites and their impact on onion production.

Chitosan nanoparticles (CS-NPs) and chitosan/polyacrylic acid hydrogel nanoparticles (CS/PAA-HNPs) were obtained by ionic gelation with tripolyphosphate anions and copolymerization of CS with acrylic acid (AA), respectively. The prepared NPs were loaded by different concentrations of copper (1, 2 and 3% with respect to CS) to obtain chitosan/copper nanocomposites (CS/Cu-NCs) and chitosan/polyacrylic acid/copper hydrogel nanocomposites (CS/PAA/Cu-HNCs). The prepared NPs and their NCs were characterized by different techniques. The swelling properties and copper release from CS/Cu-NCs and CS/PAA/Cu-HNCs were evaluated. The antibacterial activity of the prepared samples against bacteria (e.g., Staphylococcus aureus and Pseudomonas aeruginosa), fungi, and yeast were investigated. The results displayed that the copper release, as well as the swelling percentage of CS/PAA/Cu-HNCs, were higher than that of CS/Cu-NCs. Furthermore, the impact of using CuSO4, CS/Cu-NCs, and CS/PAA/Cu-HNCs as a different source of copper on chlorophyll content, vegetative growth, minerals content, and the yield of onion plants during two seasons 2016 and 2017 were studied. It was found that the yield, plant growth, and nutrient content of onion bulbs were improved using CS/PAA-HNPs, which was loaded with 75 ppm copper, as foliar spray for onion plants.