Molecular markers in oral lichen planus: A systematic review.

Oral lichen planus (OLP) is a chronic inflammatory mucosal disease that is usually detected in 0.5-2.2% of the human population. Among these, only 0.5-2.9% of the lesions progress to carcinoma. However, there are no prognostic markers available presently to recognize the increased risk in malignant transformation of the lesions. Selected markers for cell proliferation, adhesion, apoptosis and lymphocytic infiltration were analyzed by immunohistochemistry in addition to static cytometry for DNA content. The concept linking OLP and oral squamous cell carcinoma states that chronic inflammation results in crucial DNA damage, which further progresses to development of carcinoma. Even though in the past decade, enormous information has been accumulated on malignant potential of OLP, its transformation still remains unclear. Hence, the purpose of this article was to review cellular and molecular markers to understand the pathogenesis of OLP and its progression toward malignancy.

Perceived barriers to the provision of preventive care among dentists of Udaipur, India.

AIM: To investigate the practice-, patient- and dentist related barriers to the provision of preventive dental care as perceived by dentists of Udaipur city, Rajasthan, India. SETTINGS AND DESIGN: - A cross sectional descriptive survey was conducted among 120 dentists of Udaipur city, Rajasthan. MATERIAL AND METHODS: Mean Content Validity Ratio (CVR) was calculated as 0.87 based on the opinions expressed by a panel of total six academicians. Cronbach's coefficient was found to be 0.88, which showed a high internal reliability of the questionnaire. The questionnaire consisted of demographic questions and 12 specific research questions. STATISTICAL ANALYSIS USED: - Student's t-test and ANOVA test were applied for the statistical evaluation of means. Level of significance was set at 0.05. RESULTS: The barriers correlated strongly with each other (0.60 to 0.85). A significant gender difference was observed in mean sums of scores of practice and patient related barriers. Practice, dentist and patient related barriers for very much hindrance were reported by 8 to 13%, 5 to19% and 0 to 29% of the dentists respectively. A significant difference was observed among mean of sum scores of practice and patient related barriers with age and experience. Qualification was significantly related to practice related barriers. CONCLUSIONS: Perception of dentists showed that patient related barriers were found to be the foremost to the provision of preventive care. Also, dentist's attitude towards health promotion and disease prevention needs a radical transformation. Key words:Dental care, dentists, patients, perception.

Assessment of oral health among seafarers in Mundra Port, Kutch, Gujarat: a cross-sectional study.

BACKGROUND: A seafarer is a person who navigates waterborne vessels or assists as a crewmember in their operation and maintenance in all tough weather, but little research has been done to identify conditions that may lead to assess seafarer general health as well as oral health. AIM: To assess oral diseases including dental caries and periodontal conditions among seafarer's population arrived in Mundra Port, Kutch, Gujarat, India. MATERIALS AND METHODS: A descriptive cross-sectional survey was conducted to assess oral health condition of seafarer community of Mundra Taluka of Kutch District, Gujarat, India, from July 2014 to September 2014. RESULTS: Total of 385 subjects participated in the survey. Adverse habits show the overall 72.3% prevalence among the study population. Occurrence rate of caries, periodontal disease and prosthetic status were 88%, 75.1% and 6.5%, respectively. The best predictors for Decayed Missing Filled Teeth (DMFT), Community Periodontal Index (CPI) and prosthetic status were oral hygiene practices, adverse habit and educational status. CONCLUSIONS: Findings of the present study suggest that oral health condition of seafarer community was relatively poor, with high caries prevalence and poor periodontal health. This epidemiological survey has provided baseline information to underpin the implementation of oral health programmes.

Assessment and comparison of clinical dental status and its impact on oral health-related quality of life among rural and urban adults of Udaipur, India: A cross-sectional study.

BACKGROUND: Dental diseases negatively influence people's oral health-related quality of life (OHRQoL) and thus their perceived need for dental care. QoL is increasingly acknowledged as a valid, appropriate and significant indicator of service need and intervention outcomes in contemporary public health research and practice. OBJECTIVES: (1) To assess the psychometric properties of oral health impact profile-14 (OHIP-14) scale among rural and urban OHIP of Udaipur population. (2) To assess and compare clinical dental status (dental caries, periodontal disease and prosthetic status) and its impacts on OHRQoL rural and urban population of Udaipur. MATERIALS AND METHODS: A cross-sectional descriptive survey was conducted among rural (600) and urban (600) population of Udaipur that have age ranges between 20 and 79 years, chosen from outpatient department of Pacific Dental College and Hospital. The OHIP-14 was tested for validity and reliability. Chi-square, Student's t-test, analysis of variance and multiple logistic regression analysis were employed for statistical analysis. RESULTS: The Cronbach's alpha of the scale was found to be 0.85 among the rural population and 0.89 among the urban population. Prevalence of periodontal disease (community periodontal index and loss of attachment) was found greater among the rural population than the urban population. Urban population showed significantly greater proportion of subjects with prosthesis (including partial, fixed and total) as compared to the rural population. Among study population, OHIP-14 was significantly (P ≤ 0.05) associated with age, gender, presence of decayed teeth (DT), missing teeth (MT), and location. Significantly greater odds ratio (OR) (OHIP-14) were revealed among males (OR = 1.35, P = 0.02), urban residents (OR = 1.13, P = 0.002), those < 45 years of age (OR = 1.23, P = 0.01), those without DT (OR = 1.48, P = 0.002) and without MT (OR = 1.08, P = 0.03). CONCLUSION: The rural and urban study subjects had a fair clinical status. The presence of dental caries had greatest impacts on OHRQoL. In addition, rural subjects faced greater impact than urban subjects.

Relationship between oral clinical conditions and daily performances among young adults in India - a cross sectional study.

Objective of the present study was to investigate relationship between oral health-related quality of life using Oral Impacts on Daily Performances (OIDP) scale and specific clinical dental measures. A cross sectional descriptive survey was conducted among 800 students. Oral health status and impacts were assessed using WHO guidelines and OIDP index respectively. Chi square test and multiple logistic regressions were employed for statistical analysis. Participants with caries were significantly (p⩽0.05) more likely to have an impact on cleaning (OR=2.487) and sleeping and relaxing (OR=8.996). Similarly participants with oral mucosal conditions were more likely to have an impact on eating (OR=3.97), cleaning (OR=2.966) and physical activities (OR=11.190). Dental Aesthetic Index (DAI) impacted on cleaning (OR=2.134), emotional stability (OR=3.957) and social contact (OR=3.21). OIDP Index showed acceptable psychometric properties in the context of an oral health survey. Subjects presented a strong and consistent relationship between dental status and perceived impacts.

Antimicrobial Effect of Jasminum grandiflorum L. and Hibiscus rosa-sinensis L. Extracts Against Pathogenic Oral Microorganisms--An In Vitro Comparative Study.

PURPOSE: To assess and compare the antimicrobial potential and determine the minimum inhibitory concentration (MIC) of Jasminum grandiflorum and Hibiscus rosa-sinensis extracts as potential anti-pathogenic agents in dental caries. MATERIALS AND METHODS: Aqueous and ethanol (cold and hot) extracts prepared from leaves of Jasminum grandiflorum and Hibiscus rosa-sinensis were screened for in vitro antimicrobial activity against Streptococcus mutans and Lactobacillus acidophilus using the agar well diffusion method. The lowest concentration of every extract considered as the minimum inhibitory concentration (MIC) was determined for both test organisms. Statistical analysis was performed with one-way analysis of variance (ANOVA). RESULTS: At lower concentrations, hot ethanol Jasminum grandiflorum (10 µg/ml) and Hibiscus rosa-sinensis (25 µg/ml) extracts were found to have statistically significant (P≤0.05) antimicrobial activity against S. mutans and L. acidophilus with MIC values of 6.25 µg/ml and 25 µg/ml, respectively. A proportional increase in their antimicrobial activity (zone of inhibition) was observed. CONCLUSION: Both extracts were found to be antimicrobially active and contain compounds with therapeutic potential. Nevertheless, clinical trials on the effect of these plants are essential before advocating large-scale therapy.

Effect of Chocobar Ice Cream Containing Bifidobacterium on Salivary Streptococcus mutans and Lactobacilli: A Randomised Controlled Trial.

PURPOSE: To examine the effect of chocobar ice cream containing bifidobacteria on salivary mutans streptococci and lactobacilli. MATERIALS AND METHODS: A double-blind, randomised controlled trial was conducted with 30 subjects (18 to 22 years of age) divided into 2 groups, test (chocobar ice cream with probiotics) and control (chocobar ice cream without probiotics). The subjects were instructed to eat the allotted chocobar ice cream once daily for 18 days. Saliva samples collected at intervals were cultured on Mitis Salivarius agar and Rogosa agar and examined for salivary mutans streptococci and lactobacilli, respectively. The Mann-Whitney U-test, Friedman and Wilcoxon signed-rank tests were used for statistical analysis. RESULTS: Postingestion in the test group, a statistically significant reduction (p < 0.05) of salivary mutans streptococci was recorded, but a non-significant trend was seen for lactobacilli. Significant differences were was also observed between follow-ups. CONCLUSION: Short-term daily ingestion of ice cream containing probiotic bifidobacteria may reduce salivary levels of mutans streptococci in young adults.

Prevalence of malocclusion and orthodontic treatment needs among 12-15-year-old schoolchildren of fishermen of Kutch coast, Gujarat, India.

BACKGROUND: Malocclusion is one of the most common dental problems in mankind. Planning orthodontic treatment as well as an interceptive approach within a public health system requires information on the prevalence of malocclusions. AIM: The aim of the study was to assess the prevalence of malocclusion and orthodontic treatment needs among 12-15-year-old school children of fishermen of Kutch coast, Gujarat, India. MATERIALS AND METHODS: A cross-sectional descriptive survey was conducted among 947 school children offishermen of Kutch coast, Gujarat, India aged 12-15 years. The prevalence of malocclusion and orthodontic treatment needs was assessed using Dental Aesthetic Index. General information on demographic data was also recorded. A c2 test, analysis of variance (ANOVA) and Sheffe's test were employed for statistical analysis. RESULTS: Malocclusion and orthodontic treatment need was reported among 33.4% of the participants. Younger age group and female gender had significantly greater treatment need. Males and older age groups had significantly lesser prevalence of anterior crowding and largest anterior maxillary irregularity. CONCLUSIONS: Orthodontic treatment need among 33.4% calls for developing school based oral health promotion programmes for children with an inculcation of orthodontic treatment and educational programmes for parents (fishermen) addressing prevention and early interceptive treatment of malocclusion.

Correlation between oral lesions and opportunistic infections among human immunodeficiency virus - infected individuals in Indian population.

BACKGROUND: Human immunodeficiency virus (HIV) infection is a major global health problem. Tuberculosis and cryptococcal meningitis are the leading cause of death among people living with HIV. AIM: The purpose of this study was to determine whether any relationship exists between the occurrence of oral lesions and opportunistic infections among HIV-infected patients in Indian population. MATERIALS AND METHODS: A cross-sectional analytical study was performed in 232 HIV-infected persons (148 males and 84 females, aged 20-60 years, mean 33.6 ± 2.3 years). c2 test and logistic regression were used for statistical analysis. RESULTS: Oral candidiasis was the most common oral lesion seen in 28.4% males and 22.6% females of HIV-infected persons, followed by hairy leukoplakia in 27% males and 20.2% females which was statistically significant. Tuberculosis (21.6%) followed by cryptococcosis (9.9%) and pneumocystis carinii pneumonia (4.7%) were the most commonly found opportunistic infections. Logistic regression analysis revealed a significant association, between the occurrence of tuberculosis and candidiasis (OR 2.3; 95% CI 1.4-2.9), cryptococcosis and candidiasis (OR 1.4; 95% CI 1.0-1.9), and pneumocystis carinii pneumonia with hairy leukoplakia (OR 1.6; 95% CI 1.0-2.9). Mean CD4 count was also less. CONCLUSIONS: The results suggest a definite relationship in occurrence of oral lesions and opportunistic infections among HIV-infected patients.

Comparison of the Debonding Characteristics of Conventional and New Debonding Instrument used for Ceramic, Composite and Metallic Brackets - An Invitro Study.

BACKGROUND: Debonding procedure is time consuming and damaging to the enamel if performed with improper technique. Various debonding methods include: the conventional methods that use pliers or wrenches, an ultrasonic method, electrothermal devices, air pressure impulse devices, diamond burs to grind the brackets off the tooth surface and lasers. Among all these methods, using debonding pliers is most convenient and effective method but has been reported to cause damage to the teeth. Recently, a New Debonding Instrument designed specifically for ceramic and composite brackets has been introduced. As this is a new instrument, little information is available on efficacy of this instrument. The purpose of this study was to evaluate the debonding characteristics of both "the conventional debonding Pliers" and "the New debonding instrument" when removing ceramic, composite and metallic brackets. MATERIALS AND METHODS: One Hundred Thirty eight extracted maxillary premolar teeth were collected and divided into two Groups: Group A and Group B (n = 69) respectively. They were further divided into 3 subGroups (n = 23) each according to the types of brackets to be bonded. In subGroups A1 and B1{stainless steel};A2 and B2{ceramic};A3 and B3{composite}adhesive precoated maxillary premolar brackets were used. Among them {ceramic and composite} adhesive pre-coated maxillary premolar brackets were bonded. All the teeth were etched using 37% phosphoric acid for 15 seconds and the brackets were bonded using Transbond XT primer. Brackets were debonded using Conventional Debonding Plier and New Debonding Instrument (Group B). After debonding, the enamel surface of each tooth was examined under stereo microscope (10X magnifications). Amodifiedadhesive remnant index (ARI) was used to quantify the amount of remaining adhesive on each tooth. RESULTS: The observations demonstrate that the results of New Debonding Instrument for debonding of metal, ceramic and composite brackets were statistically significantly different (p = 0.04) and superior from the results of conventional debonding Pliers. CONCLUSION: The debonding efficiency of New Debonding Instrument is better than the debonding efficiency of Conventional Debonding Pliers for use of metal, ceramic and composite brackets respectively.

Cariogram Model in Assessment of Dental Caries among Mentally Challenged and Visually Impaired Individuals of Udaipur, India.

OBJECTIVES: To assess the caries risk and contribution of diet, bacteria, circumstances and susceptibility sectors among special groups in comparison to the normal group of Udaipur using the Cariogram model. MATERIALS AND METHODS: A Cariogram model was used to identify risk factors among 160 subjects (40 mentally challenged, 60 visually impaired and 60 normal healthy individuals) aged 7-36 years. Statistical analysis was done using Chi-square/ Fischer's Exact followed by Marascuilo procedure and Stepwise multiple linear regression. RESULTS: Compared to the normal group (74%), mentally challenged (33%) and visually impaired (41%) groups showed less chances of avoiding future caries. Group (R= 0.660) was found to be strongest predictor for caries risk. Susceptibility sector contributed 61% for caries risk in all the groups. CONCLUSION: The caries risk was high among special groups compared to normal group. Susceptibility sector illustrated the highest contribution for caries risk in all groups.

The oral health status and the treatment needs of salt workers at sambhar lake, jaipur, India.

BACKGROUND: Salt workers are exposed to the adversities of environmental conditions such as direct sunlight, salt dust and contact with brine, which have an impact on the health of workers. Since oral health is an integral part of the general health, we planned to determine its effect on the oral cavity. OBJECTIVES: To assess the oral health status and the treatment needs among the workers of Sambhar Salts Limited at Sambhar Lake, Jaipur, India. MATERIAL AND METHODS: A cross sectional, descriptive survey was conducted among 979 subjects (509 males; 470 females) who were aged between 19-68 years, who were the workers of Sambhar Salts Limited, Sambhar Lake, Jaipur, India. An interview on the demographic profile followed a clinical examination for recording the oral health status, based on the World Health Organization guidelines. The Chi-square test, t-test, One way Analysis of Variance and a Stepwise multiple linear regression analysis were used for the statistical analysis. RESULTS: Females had a significantly greater prevalence of dental fluorosis (71.7%) and periodontal disease (96.4%) as compared to males (p= 0.001). The mean number of healthy sextants (0.71 ± 0.09) and the mean DMFT (5.19 ± 4.11) were also significantly higher in females as compared to those in males (p=0.001). One surface filling (78.2%), followed by pulp care and restoration (76.1%) were the most prevalent treatment needs. The gender and oral hygiene practices for dental caries and periodontal disease were respectively identified as the best predictors. CONCLUSION: Considerable percentages of salt workers have demonstrated a higher prevalence of oral diseases. Higher unmet treatment needs suggest a poor accessibility and availability of oral health care, in addition to a low utilization of preventive or therapeutic oral health services.

Knowledge and attitude towards smoking among Indian students of dentistry.

UNLABELLED: Active cigarette smoking is the major cause of lung cancer and an important established cause of cardiovascular disease mortality. Risks have been shown to increase with even light or intermittent active smoking. OBJECTIVES: To assess the prevalence of smoking, age of initiation and the knowledge and attitude towards smoking among dental college students of Rajasthan, India. METHODS: A cross-sectional study was conducted among 1,383 undergraduate and postgraduate dental college students of Rajasthan using self-administered closed-ended questionnaires consisting of 14 questions divided into four sections. A chi-square test was used to determine the association between dependent and independent variables. RESULTS: Of all the participants, 777 (56.2%) were male and 606 (43.8%) were female. Among these 258 (33.2%) males and 51 (8.4%) females had tried or experimented smoking. Majority of males (24.7%) initiated this habit at a very young age, before 16 years. About 79.5% males and 72.3% females felt that it was difficult to quit smoking. According to 88.1% females and 48.7% males, people feel less comfortable at public places or social gatherings. CONCLUSION: The study has shown that the smoking prevalence among dental college students is high and has managed to contribute additional information regarding their attitude towards smoking. Comprehensive tobacco education and smoking cessation programmes should be aggressively promoted in dental settings.

Assessment of the Periodontal Status among Kota Stone Workers in Jhalawar, India.

CONTEXT: Periodontal disease is one of the most prevalent dental diseases which affects the adult population of the world, varying only in degree from mild to severe. Its prevalence, which was reported in various studies which were conducted in different parts of the globe, signifies its need for a control. AIMS: To assess the periodontal status among the Kota stone workers in Jhalawar and to provide a baseline data for the planning and evaluation of the oral health care promotion programmes. SETTINGS AND DESIGN: A cross-sectional descriptive survey was conducted among the Kota stone workers who were working in the Kota stone factories which were located in the Ricco Industrial Area in Jhalawar. METHOD AND MATERIALS: The study population comprised 420 subjects who were in the age group of 21 to 60 years. The data were collected by means of a close ended, self administered questionnaire and clinical examination with the use of the CPI index. Statistical analyses which were used: Chi-square, Student's t-test, One way Analysis of Variance (ANOVA), and Multiple Logistic Regression. RESULTS: The socio-demographic characteristics, except the gender, were found to be significantly associated with the periodontal status in the study population (P≤0.05). Multivariate analysis showed high CPI scores which were associated with males (OR=1.313), the 21-40 year age group (OR=0.548), education upto 10th class (OR=4.776), oral hygiene practices other than toothbrush with toothpaste (OR=0.774) and smoking (OR=3.994). CONCLUSION: The observations of this study indicate the need for a reorientation of the dental care services, with further emphasis on the preventive care in such a population.