Association between HOTAIR Polymorphisms and Lymphoma.

INTRODUCTION: The long non-coding RNA, HOTAIR, involved in cancer initiation and development. OBJECTIVE: The aim of present study was to investigate the association of single nucleotide polymorphisms in the HOTAIR gene with lymphoma. METHODS: We conducted a case-control study of 156 individuals with non-Hodgkin Lymphoma (NHL), 53 individuals with Hodgkin's lymphoma (HL), and 245 unrelated healthy individuals to identify the genotype frequencies of each polymorphism. Genotyping of the SNPs (rs920778 T>C, rs1899663 G>T, rs4759314 A>G and rs12826786 C>T) was carried out using the polymerase chain reaction-restriction fragment length polymorphism. RESULTS AND CONCLUSION: The finding showed that rs1899663 variant of HOTAIR gene significantly decreased the risk of NHL in codominant, dominant, over-dominant and allelic inheritance models. We did not find any association between HOTAIR rs12826786, rs920788 and rs4759314 variants and NHL. The results indicated that neither the overall chi-square comparison of the cases and controls, nor the logistic regression analysis showed any association between HOTAIR polymorphisms and HL. Conclusively, our findings showed that rs1899663 of HOTAIR significantly decreased the risk of non-Hodgkin Lymphoma.

Hippocampal microRNA-191a-5p Regulates BDNF Expression and Shows Correlation with Cognitive Impairment Induced by Paradoxical Sleep Deprivation.

The aim of this study was to investigate the effect of paradoxical sleep deprivation (PSD) on the BDNF-related miRNA expression in ovariectomized (OVX) rats. The animals were randomly divided into eight groups (control, PSD, wide platform, sham surgery, anti-miR-191, anti-miR-191/PSD, scrambled and PSD in intact). Bilateral-ovariectomy was performed one month before the experiment in the OVX rats. For the induction of 72 h of PSD, the multiple platform method was applied. The Morris water maze (MWM) test was carried out 30 min after PSD to test spatial learning and memory. Finally, the rats were euthanized 24 h after the last experiment. We quantified miR-10a-5P, miR-10b-5P, miR-125a-3p, miR-155-5p, miR-191a-5p, BDNF and TMOD-2 level using real-time PCR. BNDF protein was also measured by Western blotting. Hippocampal miR-191a and miR-155 were significantly up-regulated (P ˂ .01) and BDNF down-regulated (P ˂ .05) in the PSD group. PSD rats with up-regulated miR-191a swam longer distance and spent more time to find a hidden platform (positive correlation) and showed the lowest percentage of time and distance in the target quadrant (negative correlation). The negative correlation between miR-191a and BDNF levels in the PSD condition provided more evidence for the role of miR-191a in cognitive function. Intracerebroventricular (ICV) injection of anti-miR-191a improved the down-regulation of BDNF and attenuated PSD-induced cognitive impairment. Hippocampal BDNF is probably one of the targets of miR-191a in sleep-deprived OVX rats. Our results suggest that miR-191a may be increased in the sleep-deprived OVX rats to regulate BDNF levels.

Abnormal hippocampal miR-1b expression is ameliorated by regular treadmill exercise in the sleep-deprived female rats.

OBJECTIVES: The protective effect of regular running on sleep deprivation (SD)-induced cognitive impairment has been revealed. In this study, we focused on the effects of regular exercise, sleep deprivation and both of them together on the microRNA-1b (miR-1b) expression and their relation to the behavioral parameters and brain-derived neurotrophic factor (BDNF) expression. MATERIALS AND METHODS: We used ovariectomized (OVX) female rats. The exercise program was mild-moderate treadmill training for 4 weeks. 72 hr SD was achieved using the multiple platform method and the spatial learning and memory parameters have been evaluated by the Morris water maze (MWM) test. The levels of studied genes were quantified by real-time PCR. RESULTS: SD down-regulated pri-miR-1b, miR-1b (P˂0.05), and BDNF mRNA (P˂0.01) in the hippocampus. Furthermore, female rats under exercise conditions showed significant up-regulation of the miR-1b and BDNF mRNA (P˂0.001). In addition, miR-1b positively correlated with cognitive function (P˂0.05) and BDNF mRNA (P˂0.01). CONCLUSION: Our data demonstrated that regular treadmill exercise could reverse the down-regulation of hippocampal miR-1b, which has a probable role in the SD-induced cognitive impairment.