Video in Survey Interviews: Effects on Data Quality and Respondent Experience.

This study investigates the extent to which video technologies - now ubiquitous - might be useful for survey measurement. We compare respondents' performance and experience (n = 1,067) in live video-mediated interviews, a web survey in which prerecorded interviewers read questions, and a conventional (textual) web survey. Compared to web survey respondents, those interviewed via live video were less likely to select the same response for all statements in a battery (non-differentiation) and reported higher satisfaction with their experience but provided more rounded numerical (presumably less thoughtful) answers and selected answers that were less sensitive (more socially desirable). This suggests the presence of a live interviewer, even if mediated, can keep respondents motivated and conscientious but may introduce time pressure - a likely reason for increased rounding - and social presence - a likely reason for more socially desirable responding. Respondents "interviewed" by a prerecorded interviewer, rounded fewer numerical answers and responded more candidly than did those in the other modes, but engaged in non-differentiation more than did live video respondents, suggesting there are advantages and disadvantages for both video modes. Both live and prerecorded video seem potentially viable for use in production surveys and may be especially valuable when in-person interviews are not feasible.

INTERVIEWER EFFECTS IN LIVE VIDEO AND PRERECORDED VIDEO INTERVIEWING.

Live video (LV) communication tools (e.g., Zoom) have the potential to provide survey researchers with many of the benefits of in-person interviewing, while also greatly reducing data collection costs, given that interviewers do not need to travel and make in-person visits to sampled households. The COVID-19 pandemic has exposed the vulnerability of in-person data collection to public health crises, forcing survey researchers to explore remote data collection modes-such as LV interviewing-that seem likely to yield high-quality data without in-person interaction. Given the potential benefits of these technologies, the operational and methodological aspects of video interviewing have started to receive research attention from survey methodologists. Although it is remote, video interviewing still involves respondent-interviewer interaction that introduces the possibility of interviewer effects. No research to date has evaluated this potential threat to the quality of the data collected in video interviews. This research note presents an evaluation of interviewer effects in a recent experimental study of alternative approaches to video interviewing including both LV interviewing and the use of prerecorded videos of the same interviewers asking questions embedded in a web survey ("prerecorded video" interviewing). We find little evidence of significant interviewer effects when using these two approaches, which is a promising result. We also find that when interviewer effects were present, they tended to be slightly larger in the LV approach as would be expected in light of its being an interactive approach. We conclude with a discussion of the implications of these findings for future research using video interviewing.

A polycystin-2 protein with modified channel properties leads to an increased diameter of renal tubules and to renal cysts.

Mutations in the PKD2 gene cause autosomal-dominant polycystic kidney disease but the physiological role of polycystin-2, the protein product of PKD2, remains elusive. Polycystin-2 belongs to the transient receptor potential (TRP) family of non-selective cation channels. To test the hypothesis that altered ion channel properties of polycystin-2 compromise its putative role in a control circuit controlling lumen formation of renal tubular structures, we generated a mouse model in which we exchanged the pore loop of polycystin-2 with that of the closely related cation channel polycystin-2L1 (encoded by PKD2L1), thereby creating the protein polycystin-2poreL1. Functional characterization of this mutant channel in Xenopus laevis oocytes demonstrated that its electrophysiological properties differed from those of polycystin-2 and instead resembled the properties of polycystin-2L1, in particular regarding its permeability for Ca2+ ions. Homology modeling of the ion translocation pathway of polycystin-2poreL1 argues for a wider pore in polycystin-2poreL1 than in polycystin-2. In Pkd2poreL1 knock-in mice in which the endogenous polycystin-2 protein was replaced by polycystin-2poreL1 the diameter of collecting ducts was increased and collecting duct cysts developed in a strain-dependent fashion.

The time it takes to reveal embarrassing information in a mobile phone survey.

To explore socially desirable responding in telephone surveys, this study examines response latencies in answers to 27 questions in 319 audio-recorded iPhone interviews from Schober et al. (2015). Response latencies were compared when respondents (a) answered questions on sensitive vs. nonsensitive topics (as classified by online raters); (b) produced more vs. less socially desirable answers; and (c) were interviewed by a professional interviewer or an automated system. Respondents answered questions on sensitive topics more quickly than on nonsensitive topics, though patterns varied by question format (categorical, numerical, ordinal). Independent of question sensitivity, respondents gave less socially desirable answers more quickly when answering categorical and ordinal questions but more slowly when answering numeric questions. Respondents were particularly quicker to answer sensitive questions when asked by interviewers than by the automated system. Findings demonstrate that response times can be (differently) revealing about question and response sensitivity in a telephone survey.

When Do Misunderstandings Matter? Evidence From Survey Interviews About Smoking.

This paper examines when conceptual misalignments in dialog lead to consequential miscommunication. Two studies explore misunderstanding in survey interviews of the sort conducted by governments and social scientists, where mismeasurement can have real social costs. In 131 interviews about tobacco use, misalignment between respondents' and researchers' conceptions of ordinary expressions like "smoking" and "every day" was quantified by probing respondents' interpretations of survey terms and re-administering the survey questionnaire with standard definitions after the interview. Respondents' interpretations were surprisingly variable, and in many cases they did not match the conceptions that researchers intended them to use. More often than one might expect, this conceptual variability was consequential, leading to answers (and, in principle, to estimates of the prevalence of smoking and related attributes in the population) that would have been different had conceptualizations been aligned; for example, fully 12% of respondents gave a different answer about having smoked 100 cigarettes in their entire life when later given a standard definition. In other cases misaligned interpretations did not lead to miscommunication, in that the differences would not have led to different survey responses. Although clarification of survey terms during the interview sometimes improved conceptual alignment, this was not guaranteed; in this corpus some needed attempts at clarification were never made, some attempts did not succeed, and some seemed to make understanding worse. The findings suggest that conceptual misalignments may be more frequent in ordinary conversation than interlocutors know, and that attempts to detect and clarify them may not always work. They also suggest that at least some unresolved misunderstandings do not matter in the sense that they do not change the outcome of the communication-in this case, the survey estimates.

What About Their Performance Do Free Jazz Improvisers Agree Upon? A Case Study.

When musicians improvise freely together-not following any sort of script, predetermined harmonic structure, or "referent"-to what extent do they understand what they are doing in the same way as each other? And to what extent is their understanding privileged relative to outside listeners with similar levels of performing experience in free improvisation? In this exploratory case study, a saxophonist and a pianist of international renown who knew each other's work but who had never performed together before were recorded while improvising freely for 40 min. Immediately afterwards the performers were interviewed separately about the just-completed improvisation, first from memory and then while listening to two 5 min excerpts of the recording in order to prompt specific and detailed commentary. Two commenting listeners from the same performance community (a saxophonist and drummer) listened to, and were interviewed about, these excerpts. Some months later, all four participants rated the extent to which they endorsed 302 statements that had been extracted from the four interviews and anonymized. The findings demonstrate that these free jazz improvisers characterized the improvisation quite differently, selecting different moments to comment about and with little overlap in the content of their characterizations. The performers were not more likely to endorse statements by their performing partner than by a commenting listener from the same performance community, and their patterns of agreement with each other (endorsing or dissenting with statements) across multiple ratings-their interrater reliability as measured with Cohen's kappa-was only moderate, and not consistently higher than their agreement with the commenting listeners. These performers were more likely to endorse statements about performers' thoughts and actions than statements about the music itself, and more likely to endorse evaluatively positive than negative statements. But these kinds of statements were polarizing; the performers were more likely to agree with each other in their ratings of statements about the music itself and negative statements. As in Schober and Spiro (2014), the evidence supports a view that fully shared understanding is not needed for joint improvisation by professional musicians in this genre and that performing partners can agree with an outside listener more than with each other.

Listeners' and Performers' Shared Understanding of Jazz Improvisations.

This study explores the extent to which a large set of musically experienced listeners share understanding with a performing saxophone-piano duo, and with each other, of what happened in three improvisations on a jazz standard. In an online survey, 239 participants listened to audio recordings of three improvisations and rated their agreement with 24 specific statements that the performers and a jazz-expert commenting listener had made about them. Listeners endorsed statements that the performers had agreed upon significantly more than they endorsed statements that the performers had disagreed upon, even though the statements gave no indication of performers' levels of agreement. The findings show some support for a more-experienced-listeners-understand-more-like-performers hypothesis: Listeners with more jazz experience and with experience playing the performers' instruments endorsed the performers' statements more than did listeners with less jazz experience and experience on different instruments. The findings also strongly support a listeners-as-outsiders hypothesis: Listeners' ratings of the 24 statements were far more likely to cluster with the commenting listener's ratings than with either performer's. But the pattern was not universal; particular listeners even with similar musical backgrounds could interpret the same improvisations radically differently. The evidence demonstrates that it is possible for performers' interpretations to be shared with very few listeners, and that listeners' interpretations about what happened in a musical performance can be far more different from performers' interpretations than performers or other listeners might assume.

Down-regulation of the glucan synthase-like 6 gene (HvGsl6) in barley leads to decreased callose accumulation and increased cell wall penetration by Blumeria graminis f. sp. hordei.

The recent characterization of the polysaccharide composition of papillae deposited at the barley cell wall during infection by the powdery mildew pathogen, Blumeria graminis f. sp. hordei (Bgh), has provided new targets for the generation of enhanced disease resistance. The role of callose in papilla-based penetration resistance of crop species is largely unknown because the genes involved in the observed callose accumulation have not been identified unequivocally. We have employed both comparative and functional genomics approaches to identify the functional orthologue of AtGsl5 in the barley genome. HvGsl6 (the barley glucan synthase-like 6 gene), which has the highest sequence identity to AtGsl5, is the only Bgh-induced gene among the HvGsls examined in this study. Through double-stranded RNA interference (dsRNAi)-mediated silencing of HvGsl6, we have shown that the down-regulation of HvGsl6 is associated with a lower accumulation of papillary and wound callose and a higher susceptibility to penetration of the papillae by Bgh, compared with control lines. The results indicate that the HvGsl6 gene is a functional orthologue of AtGsl5 and is involved in papillary callose accumulation in barley. The increased susceptibility of HvGsl6 dsRNAi transgenic lines to infection indicates that callose positively contributes to the barley fungal penetration resistance mechanism.

Social Media Analyses for Social Measurement.

Demonstrations that analyses of social media content can align with measurement from sample surveys have raised the question of whether survey research can be supplemented or even replaced with less costly and burdensome data mining of already-existing or "found" social media content. But just how trustworthy such measurement can be-say, to replace official statistics-is unknown. Survey researchers and data scientists approach key questions from starting assumptions and analytic traditions that differ on, for example, the need for representative samples drawn from frames that fully cover the population. New conversations between these scholarly communities are needed to understand the potential points of alignment and non-alignment. Across these approaches, there are major differences in (a) how participants (survey respondents and social media posters) understand the activity they are engaged in; (b) the nature of the data produced by survey responses and social media posts, and the inferences that are legitimate given the data; and (c) practical and ethical considerations surrounding the use of the data. Estimates are likely to align to differing degrees depending on the research topic and the populations under consideration, the particular features of the surveys and social media sites involved, and the analytic techniques for extracting opinions and experiences from social media. Traditional population coverage may not be required for social media content to effectively predict social phenomena to the extent that social media content distills or summarizes broader conversations that are also measured by surveys.

Improving care for victims of violence in resource-poor settings such as Lusaka, Zambia: results of a low-budget intervention.

UNLABELLED: Health professionals from Zambia and Austria conducted a low-cost intervention in Lusaka, Zambia, intended to improve care outcomes for victims of interpersonal violence (IPEV). It was designed to build on existing health and social services infrastructures. During 1 year, 174 victims of IPEV seen at the Lusaka University Hospital emergency room were interviewed by medical students. An intervention included training for medical and social service personnel and distribution at key locations of printed materials on services available to IPEV victims. Postintervention data analysis revealed that victims of IPEV had improved understanding of available social services, and victims' confidence was increased about receiving additional help and articulating health concerns. Other benefits: improved visibility, networking among partner organisations; new descriptive data about IPEV victims; improved cross-cultural understanding among medical participants. RECOMMENDATION: low-cost interventions should continue to be explored to improve care for victims of IPEV in resource-poor settings.

Comprehension and engagement in survey interviews with virtual agents.

This study investigates how an onscreen virtual agent's dialog capability and facial animation affect survey respondents' comprehension and engagement in "face-to-face" interviews, using questions from US government surveys whose results have far-reaching impact on national policies. In the study, 73 laboratory participants were randomly assigned to respond in one of four interviewing conditions, in which the virtual agent had either high or low dialog capability (implemented through Wizard of Oz) and high or low facial animation, based on motion capture from a human interviewer. Respondents, whose faces were visible to the Wizard (and videorecorded) during the interviews, answered 12 questions about housing, employment, and purchases on the basis of fictional scenarios designed to allow measurement of comprehension accuracy, defined as the fit between responses and US government definitions. Respondents answered more accurately with the high-dialog-capability agents, requesting clarification more often particularly for ambiguous scenarios; and they generally treated the high-dialog-capability interviewers more socially, looking at the interviewer more and judging high-dialog-capability agents as more personal and less distant. Greater interviewer facial animation did not affect response accuracy, but it led to more displays of engagement-acknowledgments (verbal and visual) and smiles-and to the virtual interviewer's being rated as less natural. The pattern of results suggests that a virtual agent's dialog capability and facial animation differently affect survey respondents' experience of interviews, behavioral displays, and comprehension, and thus the accuracy of their responses. The pattern of results also suggests design considerations for building survey interviewing agents, which may differ depending on the kinds of survey questions (sensitive or not) that are asked.

Precision and Disclosure in Text and Voice Interviews on Smartphones.

As people increasingly communicate via asynchronous non-spoken modes on mobile devices, particularly text messaging (e.g., SMS), longstanding assumptions and practices of social measurement via telephone survey interviewing are being challenged. In the study reported here, 634 people who had agreed to participate in an interview on their iPhone were randomly assigned to answer 32 questions from US social surveys via text messaging or speech, administered either by a human interviewer or by an automated interviewing system. 10 interviewers from the University of Michigan Survey Research Center administered voice and text interviews; automated systems launched parallel text and voice interviews at the same time as the human interviews were launched. The key question was how the interview mode affected the quality of the response data, in particular the precision of numerical answers (how many were not rounded), variation in answers to multiple questions with the same response scale (differentiation), and disclosure of socially undesirable information. Texting led to higher quality data-fewer rounded numerical answers, more differentiated answers to a battery of questions, and more disclosure of sensitive information-than voice interviews, both with human and automated interviewers. Text respondents also reported a strong preference for future interviews by text. The findings suggest that people interviewed on mobile devices at a time and place that is convenient for them, even when they are multitasking, can give more trustworthy and accurate answers than those in more traditional spoken interviews. The findings also suggest that answers from text interviews, when aggregated across a sample, can tell a different story about a population than answers from voice interviews, potentially altering the policy implications from a survey.

Probabilistic shortest path tractography in DTI using Gaussian Process ODE solvers.

Tractography in diffusion tensor imaging estimates connectivity in the brain through observations of local diffusivity. These observations are noisy and of low resolution and, as a consequence, connections cannot be found with high precision. We use probabilistic numerics to estimate connectivity between regions of interest and contribute a Gaussian Process tractography algorithm which allows for both quantification and visualization of its posterior uncertainty. We use the uncertainty both in visualization of individual tracts as well as in heat maps of tract locations. Finally, we provide a quantitative evaluation of different metrics and algorithms showing that the adjoint metric (8] combined with our algorithm produces paths which agree most often with experts.

Jazz improvisers' shared understanding: a case study.

To what extent and in what arenas do collaborating musicians need to understand what they are doing in the same way? Two experienced jazz musicians who had never previously played together played three improvisations on a jazz standard ("It Could Happen to You") on either side of a visual barrier. They were then immediately interviewed separately about the performances, their musical intentions, and their judgments of their partner's musical intentions, both from memory and prompted with the audiorecordings of the performances. Statements from both (audiorecorded) interviews as well as statements from an expert listener were extracted and anonymized. Two months later, the performers listened to the recordings and rated the extent to which they endorsed each statement. Performers endorsed statements they themselves had generated more often than statements by their performing partner and the expert listener; their overall level of agreement with each other was greater than chance but moderate to low, with disagreements about the quality of one of the performances and about who was responsible for it. The quality of the performances combined with the disparities in agreement suggest that, at least in this case study, fully shared understanding of what happened is not essential for successful improvisation. The fact that the performers endorsed an expert listener's statements more than their partner's argues against a simple notion that performers' interpretations are always privileged relative to an outsider's.

The detection of platelet antibodies by simultaneous analysis of specific platelet antibodies and the monoclonal antibody-specific immobilization of platelet antigens: an interlaboratory comparison.

BACKGROUND: Glycoprotein (GP)-specific platelet (PLT) antibodies can cause allo- or autoimmune thrombocytopenia. Their detection is of high diagnostic value. The simultaneous analysis of specific PLT antibodies (SASPA) assay is based on simultaneous detection of various PLT-specific antibodies by flow cytometry and has entered routine use in our Mannheim institution. In this study, we performed an interlaboratory comparison investigation of PLT-specific antibodies using SASPA versus the "gold standard," the monoclonal antibody-specific immobilization of PLT antigen (MAIPA) assay. STUDY DESIGN AND METHODS: Sera from 194 patients with suspected PLT allo- or autoantibodies were tested against GPIIb/IIIa, IX, Ia/IIa, IV, and HLA Class I by SASPA (in Mannheim) and MAIPA (in Vienna). All data were reported blinded to those from the respective other method. Sensitivity studies included dilution studies with known antibodies against HPA-1a, -1b, -3b, -5b, and -15b and HLA Class I. RESULTS: Overall, results were concordant in 78.9%. The specificity and sensitivity of SASPA, based on the MAIPA results, were 97.3 and 86.3%, respectively, for the detection of alloantibodies. The respective results for the detection of autoantibodies were 95.3 and 44.9%. Serial dilution experiments with sera containing anti-HPA1a, -1b, -3b, -5b, and -15b and anti-HLA Class I revealed a higher sensitivity of the SASPA assay with all alloantibodies. CONCLUSION: In this first blind interlaboratory comparison, SASPA yielded similar results to those of MAIPA. The SASPA assay may be superior to the MAIPA assay for the detection of weak alloantibodies while simultaneous detection of a variety of antibody specificities or immunoglobulin classes and the need of fewer PLTs are obvious advantages.

Analysis of the (1,3)-beta-D-glucan synthase gene family of barley.

Callose consists mostly of (1,3)-beta-D-glucan and is synthesised in many plant tissues during growth and development, where it is believed to play a fundamental role in cell plate formation during cell division. Callose deposition also represents an important response to pathogen attack, wounding and to various abiotic stresses. Here, the transcription patterns of members of the callose synthase gene family from barley (Hordeum vulgare) were defined. Thus, fragments of six barley (1,3)-beta-D-glucan synthase-like (GSL) cDNAs were obtained by PCR amplification using primers designed to barley expressed sequence tag (EST) sequences. The HvGSL genes, designated HvGSL2 to HvGSL7, were mapped to individual loci that were distributed across the barley genome on chromosomes 3H, 4H, 6H and 7H. The HvGSL1 gene has been isolated and characterised previously. Transcript levels for all the genes were analysed by quantitative real-time PCR in a range of barley tissues and organs, at various developmental stages. High levels of transcript for many of the HvGSL genes were detected in endosperm during the early stages of grain development, when cellularisation of the endosperm was occurring and it is likely that many of the genes participate in this process. Transcripts of HvGSL1 and HvGSL5 mRNAs were significantly more abundant than other GSL mRNAs in the roots of young seedlings, while HvGSL7 mRNA was detected at relatively high levels along the length of two week old shoots.

Bioenergetic-based neuroprotection and glaucoma.

Primary open-angle glaucoma (POAG) is a pressure-sensitive optic neuropathy which results in the death of retinal ganglion cells and causes associated loss of vision. Presently, the only accepted treatment strategy is to lower the intraocular pressure; however, for some patients this is insufficient to prevent progressive disease. Although the pathogenesis of POAG remains unclear, there is considerable evidence that energy failure at the optic nerve head may be involved. Neuroprotection, a strategy which directly enhances the survival of neurons, is desirable, but remains clinically elusive. One particular form of neuroprotection involves the notion of enhancing the energy supply of neurons. These 'bioenergetic' methods of neuroprotection have proven successful in animal models of other neurodegenerative diseases and conditions, including Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis and traumatic brain injury, but have been relatively unexplored in glaucoma models. This review focuses on some of the potential approaches for bioenergetic neuroprotection in the retina, including increasing the energy buffering capacity of damaged cells, decreasing the permeability of the mitochondrial membrane pore and free radical scavenging.

Sterol binding assay using surface plasmon fluorescence spectroscopy.

We describe the design of a novel in vitro assay to study the interaction of soluble proteins with small hydrophobic sterol ligands. The sterol molecules are incorporated in an artificial membrane system in order to mimic their arrangement found in a biomembrane. The artificial membrane setup is monitored in real time by surface plasmon spectroscopy. Binding of fluorescently labeled soluble protein is observed by optical detection with surface plasmon enhanced fluorescence spectroscopy. By application of the novel assay, we demonstrate that four different oxidized sterol molecules are specifically recognized by the yeast protein Osh5p, a presumed oxysterol binding protein. Osh5p from yeast is the first oxysterol binding protein homologue for which oxysterol binding is shown with this new technique. With the design of our novel in vitro oxysterol binding assay, we have solved the technically challenging difficulty of presenting hydrophobic ligands to hydrophilic proteins in aqueous media.

Just how aligned are interlocutors' representations?

Conversational partners' representations may be less aligned than they appear even when interlocutors believe they have successfully understood each other, as data from a series of experiments on surveys about facts and behaviors suggest. Although the goal of a mechanistic psychology of dialogue is laudable, the ultimate model is likely to require far greater specification of individual and contextual variability.