RESUMEN
DEK is an abundant and ubiquitous chromatin protein. Here we investigate whether DEK is regularly distributed in the chromatin of human HeLa cells. We show that DEK appears to be excluded from the heterochromatic compartment. However, DEK seems to colocalize with a subfraction of chromatin bearing acetylated histone H4. We examined certain DNA sequences in specifically immunoprecipitated chromatin for four selected human genes. We found that most of the investigated gene sequences were moderately enriched in immunoprecipitated chromatin. In contrast, a promoter-proximal element of the human TOP1 gene was highly enriched in the chromatin immunoprecipitates. This enrichment was lost when cells were treated with alpha-amanitin showing that DEK binds to this particular site only when the TOP1 gene is actively expressed. Our conclusion is that DEK could serve as an architectural protein at the promoter or enhancer sites of a subfraction of human genes.
Asunto(s)
Cromatina/genética , Cromatina/metabolismo , Proteínas Cromosómicas no Histona/genética , Proteínas Cromosómicas no Histona/metabolismo , Proteínas Oncogénicas/genética , Proteínas Oncogénicas/metabolismo , Regiones Promotoras Genéticas/genética , Células 3T3 , Animales , Sitios de Unión , Células HeLa , Humanos , Ratones , Proteínas de Unión a Poli-ADP-Ribosa , Unión ProteicaRESUMEN
DEK is an abundant chromatin protein in metazoans reaching copy numbers of several millions/nucleus. Previous work has shown that human DEK, a protein of 375 amino acids, has two functional DNA-binding domains, of which one resides in a central part of the molecule and contains sequences corresponding to the scaffold attachment factor-box (SAF-box) domain as found in a growing number of nuclear proteins. Isolated SAF-box peptides (amino acids 137-187) bind weakly to DNA in solution, but when many SAF-box peptides are brought into close proximity on the surface of Sephadex beads, cooperative effects lead to a high affinity to DNA. Furthermore, a peptide (amino acids 87-187) that includes a sequence on the N-terminal side of the SAF-box binds efficiently to DNA. This peptide prefers four-way junction DNA over straight DNA and induces supercoils in relaxed circular DNA just like the full-length DEK. Interestingly, however, the 87-187 amino acid peptide introduces negative supercoils in contrast to the full-length DEK, which is known to introduce positive supercoils. We found that two adjacent regions (amino acids 68-87 and 187-250) are necessary for the formation of positive supercoils. Our data contribute to the ongoing characterization of the abundant and ubiquitous DEK chromatin protein.
Asunto(s)
Proteínas Cromosómicas no Histona/química , Proteínas Oncogénicas/química , Secuencia de Aminoácidos , Sitios de Unión , Cromatina/metabolismo , Proteínas Cromosómicas no Histona/metabolismo , ADN/metabolismo , ADN Cruciforme/metabolismo , ADN Superhelicoidal/química , Humanos , Datos de Secuencia Molecular , Proteínas Oncogénicas/metabolismo , Péptidos/metabolismo , Proteínas de Unión a Poli-ADP-Ribosa , Estructura Terciaria de ProteínaRESUMEN
The protein DEK is an abundant and ubiquitous chromatin protein in multicellular organisms (not in yeast). It is expressed in more than a million copies/nucleus of rapidly proliferating mammalian cells. DEK has two DNA binding modules of which one includes a SAP box, a sequence motif that DEK shares with a number of other chromatin proteins. DEK has no apparent affinity to specific DNA sequences, but preferentially binds to superhelical and cruciform DNA, and induces positive supercoils into closed circular DNA. The available evidence strongly suggests that DEK could function as an architectural protein in chromatin comparable to the better known classic architectural chromatin proteins, the high-mobility group or HMG proteins.
Asunto(s)
Cromatina/genética , Proteínas Cromosómicas no Histona/genética , Proteínas Oncogénicas/genética , Animales , Proteínas Cromosómicas no Histona/química , Proteínas Cromosómicas no Histona/metabolismo , Mapeo Cromosómico , Cromosomas Humanos Par 6/genética , Proteínas del Grupo de Alta Movilidad/genética , Humanos , Leucemia Mieloide Aguda/genética , Nucleoproteínas/genética , Nucleoproteínas/metabolismo , Proteínas Oncogénicas/química , Proteínas Oncogénicas/metabolismo , Oncogenes , Proteínas de Unión a Poli-ADP-Ribosa , Translocación GenéticaRESUMEN
DEK was originally described as a proto-oncogene protein and is now known to be a major component of metazoan chromatin. DEK is able to modify the structure of DNA by introducing supercoils. In order to find interaction partners and functional domains of DEK, we performed yeast two-hybrid screens and mutational analyses. Two-hybrid screening yielded C-terminal fragments of DEK, suggesting that DEK is able to multimerize. We could localize the domain to amino acids 270 to 350 and show that multimerization is dependent on phosphorylation by CK2 kinase in vitro. We also found two DNA binding domains of DEK, one on a fragment including amino acids 87 to 187 and containing the SAF-box DNA binding motif, which is located between amino acids 149 and 187. This region is sufficient to introduce supercoils into DNA. The second DNA binding domain is located between amino acids 270 and 350 and thus overlaps the multimerization domain. We show that the two DNA-interacting domains differ in their binding properties and in their abilities to respond to CK2 phosphorylation.
Asunto(s)
Proteínas Cromosómicas no Histona/metabolismo , Proteínas Oncogénicas/metabolismo , Secuencia de Aminoácidos , Sitios de Unión , Quinasa de la Caseína II , Proteínas Cromosómicas no Histona/química , Proteínas Cromosómicas no Histona/fisiología , ADN Superhelicoidal/metabolismo , Dimerización , Células HeLa , Humanos , Proteínas Oncogénicas/química , Proteínas Oncogénicas/fisiología , Fosforilación , Proteínas de Unión a Poli-ADP-Ribosa , Proteínas Serina-Treonina Quinasas/metabolismo , Estructura Terciaria de Proteína/fisiología , Proto-Oncogenes Mas , Técnicas del Sistema de Dos HíbridosRESUMEN
Pax transcription factors are found in animals, from simple sponges to insects and vertebrates. The defining feature of Pax proteins is the DNA-binding paired domain (PD), which consists of two helix-turn-helix subdomains, joined with a linker region. Despite high specificity in vivo, the paired domains of different Pax proteins bind similar consensus DNA sequences in vitro. Using bandshift techniques, we show here that the paired domain of the Acropora millepora PaxD protein, which unambiguously belongs to the Pax3/7 group, does not bind to three defined paired domain-binding sites. Domain swapping experiments and site-directed mutagenesis identified two amino acid residues in the linker region of the paired domain as critical to DNA binding; G70 and S71 are highly conserved in Pax proteins, but differ in PaxD (L70 and N71). The PaxD data thus highlight the importance of the linker region, and particularly G70 and S71, in DNA binding by Pax proteins.