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1.
Nat Commun ; 13(1): 6898, 2022 11 12.
Artículo en Inglés | MEDLINE | ID: mdl-36371405

RESUMEN

Stress can cause overconsumption of palatable high caloric food. Despite the important role of stress eating in obesity and (binge) eating disorders, its underlying neural mechanisms remain unclear. Here we demonstrate in mice that stress alters lateral hypothalamic area (LHA) control over the ventral tegmental area (VTA), thereby promoting overconsumption of palatable food. Specifically, we show that glutamatergic LHA neurons projecting to the VTA are activated by social stress, after which their synapses onto dopamine neurons are potentiated via AMPA receptor subunit alterations. We find that stress-driven strengthening of these specific synapses increases LHA control over dopamine output in key target areas like the prefrontal cortex. Finally, we demonstrate that while inducing LHA-VTA glutamatergic potentiation increases palatable fat intake, reducing stress-driven potentiation of this connection prevents such stress eating. Overall, this study provides insights in the neural circuit adaptations caused by stress that drive overconsumption of palatable food.


Asunto(s)
Área Hipotalámica Lateral , Área Tegmental Ventral , Ratones , Animales , Neuronas Dopaminérgicas , Sinapsis , Receptores AMPA
2.
Mol Psychiatry ; 27(4): 2068-2079, 2022 04.
Artículo en Inglés | MEDLINE | ID: mdl-35177825

RESUMEN

Forebrain dopamine-sensitive (dopaminoceptive) neurons play a key role in movement, action selection, motivation, and working memory. Their activity is altered in Parkinson's disease, addiction, schizophrenia, and other conditions, and drugs that stimulate or antagonize dopamine receptors have major therapeutic applications. Yet, similarities and differences between the various neuronal populations sensitive to dopamine have not been systematically explored. To characterize them, we compared translating mRNAs in the dorsal striatum and nucleus accumbens neurons expressing D1 or D2 dopamine receptor and prefrontal cortex neurons expressing D1 receptor. We identified genome-wide cortico-striatal, striatal D1/D2 and dorso/ventral differences in the translating mRNA and isoform landscapes, which characterize dopaminoceptive neuronal populations. Expression patterns and network analyses identified novel transcription factors with presumptive roles in these differences. Prostaglandin E2 (PGE2) was a candidate upstream regulator in the dorsal striatum. We pharmacologically explored this hypothesis and showed that misoprostol, a PGE2 receptor agonist, decreased the excitability of D2 striatal projection neurons in slices, and diminished their activity in vivo during novel environment exploration. We found that misoprostol also modulates mouse behavior including by facilitating reversal learning. Our study provides powerful resources for characterizing dopamine target neurons, new information about striatal gene expression patterns and regulation. It also reveals the unforeseen role of PGE2 in the striatum as a potential neuromodulator and an attractive therapeutic target.


Asunto(s)
Dinoprostona , Misoprostol , Animales , Cuerpo Estriado/metabolismo , Dinoprostona/metabolismo , Dinoprostona/farmacología , Dopamina/metabolismo , Neuronas Dopaminérgicas/metabolismo , Exones , Expresión Génica , Ratones , Misoprostol/metabolismo , Misoprostol/farmacología , ARN Mensajero/metabolismo , Receptores de Dopamina D1/metabolismo
3.
Neurobiol Learn Mem ; 173: 107265, 2020 09.
Artículo en Inglés | MEDLINE | ID: mdl-32531423

RESUMEN

Kleefstra syndrome is a disorder caused by a mutation in the EHMT1 gene characterized in humans by general developmental delay, mild to severe intellectual disability and autism. Here, we characterized cumulative memory in the Ehmt1+/- mouse model using the Object Space Task. We combined conventional behavioral analysis with automated analysis by deep-learning networks, a session-based computational learning model, and a trial-based classifier. Ehmt1+/- mice showed more anxiety-like features and generally explored objects less, but the difference decreased over time. Interestingly, when analyzing memory-specific exploration, Ehmt1+/- show increased expression of cumulative memory, but a deficit in a more simple, control memory condition. Using our automatic classifier to differentiate between genotypes, we found that cumulative memory features are better suited for classification than general exploration differences. Thus, detailed behavioral classification with the Object Space Task produced a more detailed behavioral phenotype of the Ehmt1+/- mouse model.


Asunto(s)
Conducta Animal/fisiología , Anomalías Craneofaciales/fisiopatología , Conducta Exploratoria/fisiología , Cardiopatías Congénitas/fisiopatología , Discapacidad Intelectual/fisiopatología , Memoria/fisiología , Animales , Deleción Cromosómica , Cromosomas Humanos Par 9/genética , Anomalías Craneofaciales/genética , Aprendizaje Profundo , Modelos Animales de Enfermedad , Cardiopatías Congénitas/genética , N-Metiltransferasa de Histona-Lisina/genética , Discapacidad Intelectual/genética , Masculino , Ratones
4.
Proc Natl Acad Sci U S A ; 116(14): 7077-7082, 2019 04 02.
Artículo en Inglés | MEDLINE | ID: mdl-30877244

RESUMEN

Extensive evidence indicates that the basolateral amygdala (BLA) interacts with other brain regions in mediating stress hormone and emotional arousal effects on memory consolidation. Brain activation studies have shown that arousing conditions lead to the activation of large-scale neural networks and several functional connections between brain regions beyond the BLA. Whether such distal interactions on memory consolidation also depend on BLA activity is not as yet known. We investigated, in male Sprague-Dawley rats, whether BLA activity enables prelimbic cortex (PrL) interactions with the anterior insular cortex (aIC) and dorsal hippocampus (dHPC) in regulating glucocorticoid effects on different components of object recognition memory. The glucocorticoid receptor (GR) agonist RU 28362 administered into the PrL, but not infralimbic cortex, immediately after object recognition training enhanced 24-hour memory of both the identity and location of the object via functional interactions with the aIC and dHPC, respectively. Importantly, posttraining inactivation of the BLA by the noradrenergic antagonist propranolol abolished the effect of GR agonist administration into the PrL on memory enhancement of both the identity and location of the object. BLA inactivation by propranolol also blocked the effect of GR agonist administration into the PrL on inducing changes in neuronal activity within the aIC and dHPC during the postlearning consolidation period as well as on structural changes in spine morphology assessed 24 hours later. These findings provide evidence that BLA noradrenergic activity enables functional interactions between the PrL and the aIC and dHPC in regulating stress hormone and emotional arousal effects on memory.


Asunto(s)
Androstanoles/farmacología , Complejo Nuclear Basolateral/metabolismo , Corteza Cerebral/metabolismo , Glucocorticoides/metabolismo , Memoria/efectos de los fármacos , Red Nerviosa/metabolismo , Receptores de Glucocorticoides/agonistas , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Receptores de Glucocorticoides/metabolismo
5.
Nature ; 563(7731): 397-401, 2018 11.
Artículo en Inglés | MEDLINE | ID: mdl-30405240

RESUMEN

Dopamine modulates medial prefrontal cortex (mPFC) activity to mediate diverse behavioural functions1,2; however, the precise circuit computations remain unknown. One potentially unifying model by which dopamine may underlie a diversity of functions is by modulating the signal-to-noise ratio in subpopulations of mPFC neurons3-6, where neural activity conveying sensory information (signal) is amplified relative to spontaneous firing (noise). Here we demonstrate that dopamine increases the signal-to-noise ratio of responses to aversive stimuli in mPFC neurons projecting to the dorsal periaqueductal grey (dPAG). Using an electrochemical approach, we reveal the precise time course of pinch-evoked dopamine release in the mPFC, and show that mPFC dopamine biases behavioural responses to aversive stimuli. Activation of mPFC-dPAG neurons is sufficient to drive place avoidance and defensive behaviours. mPFC-dPAG neurons display robust shock-induced excitations, as visualized by single-cell, projection-defined microendoscopic calcium imaging. Finally, photostimulation of dopamine terminals in the mPFC reveals an increase in the signal-to-noise ratio in mPFC-dPAG responses to aversive stimuli. Together, these data highlight how dopamine in the mPFC can selectively route sensory information to specific downstream circuits, representing a potential circuit mechanism for valence processing.


Asunto(s)
Reacción de Prevención/fisiología , Dopamina/metabolismo , Sustancia Gris Periacueductal/citología , Sustancia Gris Periacueductal/fisiología , Corteza Prefrontal/citología , Corteza Prefrontal/fisiología , Animales , Señalización del Calcio , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Vías Nerviosas , Ratas , Ratas Long-Evans , Relación Señal-Ruido , Análisis de la Célula Individual , Cola (estructura animal)
6.
Drug Alcohol Depend ; 142: 314-24, 2014 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-25060961

RESUMEN

BACKGROUND: Persistent drug seeking despite harmful consequences is a defining characteristic of addiction. Recent preclinical studies have demonstrated the occurrence of this hallmark feature of addictive behaviour in rodents. For example, it has been shown that the ability of an aversive conditioned stimulus (CS) to suppress cocaine seeking was diminished after an extended self-administration history. The present study aimed to optimize the experimental conditions to examine conditioned suppression of sucrose and cocaine seeking in rats, and its dependence on the longevity of self-administration experience. METHODS: We investigated whether conditioned suppression depends on the intensity and quantity of footshocks during conditioning. In addition, the effects of CS omission, extinction and reconditioning were investigated, as well as the influence of the CS interval sequence on conditioned suppression. We also compared conditioned suppression after a limited and extended sucrose or cocaine self-administration history. RESULTS: We found that conditioned suppression depended on the intensity rather than the quantity of footshocks, whereby a higher footshock intensity was necessary to induce suppression of cocaine seeking compared to sucrose seeking. Conditioned suppression was most pronounced when the test started with presentation of the aversive CS, and conditioned suppression could be extinguished and reacquired. In addition, conditioned suppression of cocaine, but not sucrose seeking was reduced after extended self-administration experience. CONCLUSIONS: These data provide a detailed analysis of conditioned suppression of cocaine and sucrose seeking. Importantly, we confirm the usefulness of conditioned suppression to study persistent drug seeking after prolonged drug self-administration.


Asunto(s)
Reacción de Prevención/fisiología , Cocaína/administración & dosificación , Condicionamiento Operante/fisiología , Inhibidores de Captación de Dopamina/administración & dosificación , Comportamiento de Búsqueda de Drogas/fisiología , Animales , Reacción de Prevención/efectos de los fármacos , Conducta Adictiva , Conducta Animal/efectos de los fármacos , Conducta Animal/fisiología , Conducta Compulsiva , Condicionamiento Operante/efectos de los fármacos , Comportamiento de Búsqueda de Drogas/efectos de los fármacos , Masculino , Ratas , Ratas Wistar , Esquema de Refuerzo , Autoadministración
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