Modification of sorghum starch as a function of pullulanase hydrolysis and infrared treatment.

Papain-pretreated sorghum grains were modified by using pullulanase and infrared (IR) irradiation to decrease starch digestibility. An optimum synergistic effect was found under conditions of pullulanase (1 U/ml/5h) and IR (220 oC/3 min) treatment, which produced modified corneous endosperm starch with 0.022 hydrolysis rate, 42.58 hydrolysis index, and 0.468 potential digestibility. The modification increased amylose content and crystallinity up to 31.31 % and 62.66 %, respectively. However, the starch modification decreased its swelling power, solubility index, and pasting properties. FTIR revealed an increase in the ratio of 1047/1022 and a decrease in 1022/995, indicating the formation of a more orderly structure. The debranching effect of pullulanase was stabilized by the IR radiation amplifying its effect on starch digestibility. Therefore, the combination of debranching and infrared treatment could be an efficient method to produce 'tailor-made' starch, that can be further utilized in food industries to manufacture food for target population.

Functional modification of grain proteins by dual approaches: Current progress, challenges, and future perspectives.

Protein modification is a practical strategy to enhance the functional characteristics of proteins and broaden their commercial applications. Various chemical (e.g., pH-shifting, deamidation, succinylation), physical (e.g., sonication, high-speed shearing), or biological (e.g., microbial transglutaminase cross-linking, enzymatic hydrolysis) modification methods have frequently been employed to improve the functionality of native grain proteins. However, progress in intensification has led to the emergence of advanced methodologies, which involve the combination of modification techniques, generally known as "Dual Modification". This paper aims to comprehensively review the most recent researches focusing on the effects of dual modification on the functionality of grain proteins. Particular emphasis is given to elucidate the impact of this technique on physicochemical and structural properties. Furthermore, existing challenges and limitations associated with the utilization of this approach are highlighted, and prospects are proposed.

In situ thermal modification of kafirin using infrared radiations and microwaves.

BACKGROUND: Kafirin is a prolamin protein located in the corneous endosperm of sorghum. The conventional thermal processing of kafirin reduces its solubility, which limits its utilization in the food industry. Therefore, the study was aimed to investigate the effect of in situ thermal modification of kafirin using two different electromagnetic thermal treatments, namely infrared (IR) and microwave (MW) radiation, on the physicochemical, structural, thermal, and antioxidant properties. RESULTS: The results demonstrated that both the thermal modifications improved yield, purity, and solubility of the kafirin with a decrease in hydrophobicity. However, IR-treated samples showed higher solubility (910.67 g kg-1 ) and lower hydrophobicity (387.67). The IR modifications also improved the ratio of α helix/ß sheets to a great extent. The alterations in the disulfide content were concomitant with the improvement in the thermal stability of kafirin. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed variations in the band intensities of ß- and γ-kafirin, indicating alterations in the kafirin subunits. Morphological examination of kafirin revealed surface withering and agglomeration. Notably, IR treatment improved the antioxidant activity more efficiently (from 32.11% to 74.05%). CONCLUSION: Although both the IR and MW treatments modified kafirin, the effect seemed to be more pronounced in IR modification. The IR-modified kafirin had better solubility and lesser hydrophobicity than MW-modified kafirin. The physicochemical and structural changes induced by IR treatment improved the biological activity of kafirin, in terms of antioxidant activity. Therefore, it was concluded that the in situ IR modification of kafirin can alter its characteristic properties, improving its potential as a food ingredient. © 2021 Society of Chemical Industry.