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Reduced MK801 binding in neocortical neurons after AAV-mediated transfections with NMDA-R1 antisense cDNA.

Shafron, D R; Simpkins, C E; Jebelli, B; Day, A L; Meyer, E M.

Brain Res ; 784(1-2): 325-8, 1998 Feb 16.

Artículo en Inglés | MEDLINE | ID: mdl-9518673

RESUMEN

Two adeno-associated virus (AAV)-derived plasmids were constructed with portions of the N-methyl-d-aspartic acid-R1 (NMDA-R1) receptor subunit downstream from the AAV p40-(pJDT95dlk-aR1) or cytomegalovirus (CMV) promoter (pTRUF3-aR1) in an antisense orientation. Each plasmid drove expression of antisense NMDA-R1 in primary rat neocortical neuronal cultures 4 days after transfection as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Transfection with pTRUF3-aR1 (2x4 microgram) but not with pJDT95dlk-aR1 decreased neuronal [3H]MK-801 binding in a dose-dependent manner.

Asunto(s)

Corteza Cerebral/metabolismo , Citomegalovirus/genética , Maleato de Dizocilpina/metabolismo , Vectores Genéticos/genética , Neuronas/metabolismo , Oligonucleótidos Antisentido/farmacología , Receptores de N-Metil-D-Aspartato/genética , Receptores de N-Metil-D-Aspartato/metabolismo , Animales , Células Cultivadas , Corteza Cerebral/citología , Ratones , Plásmidos/genética , Reacción en Cadena de la Polimerasa , Ratas , Transfección

RESUMEN

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