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1.
J Cell Sci ; 137(14)2024 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-38940198

RESUMEN

TMEM16F (also known as ANO6), a Ca2+-activated lipid scramblase (CaPLSase) that dynamically disrupts lipid asymmetry, plays a crucial role in various physiological and pathological processes, such as blood coagulation, neurodegeneration, cell-cell fusion and viral infection. However, the mechanisms through which it regulates these processes remain largely elusive. Using endothelial cell-mediated angiogenesis as a model, here we report a previously unknown intracellular signaling function of TMEM16F. We demonstrate that TMEM16F deficiency impairs developmental retinal angiogenesis in mice and disrupts angiogenic processes in vitro. Biochemical analyses indicate that the absence of TMEM16F enhances the plasma membrane association of activated Src kinase. This in turn increases VE-cadherin phosphorylation and downregulation, accompanied by suppressed angiogenesis. Our findings not only highlight the role of intracellular signaling by TMEM16F in endothelial cells but also open new avenues for exploring the regulatory mechanisms for membrane lipid asymmetry and their implications in disease pathogenesis.


Asunto(s)
Anoctaminas , Células Endoteliales , Transducción de Señal , Animales , Anoctaminas/metabolismo , Anoctaminas/genética , Ratones , Humanos , Células Endoteliales/metabolismo , Familia-src Quinasas/metabolismo , Familia-src Quinasas/genética , Neovascularización Fisiológica , Fosforilación , Cadherinas/metabolismo , Antígenos CD/metabolismo , Antígenos CD/genética , Células Endoteliales de la Vena Umbilical Humana/metabolismo , Membrana Celular/metabolismo , Ratones Endogámicos C57BL , Ratones Noqueados , Angiogénesis , Proteínas de Transferencia de Fosfolípidos
2.
bioRxiv ; 2023 Aug 20.
Artículo en Inglés | MEDLINE | ID: mdl-37645870

RESUMEN

Dynamic loss of lipid asymmetry through the activation of TMEM16 Ca2+-activated lipid scramblases (CaPLSases) has been increasingly recognized as an essential membrane event in a wide range of physiological and pathological processes, including blood coagulation, microparticle release, bone development, pain sensation, cell-cell fusion, and viral infection. Despite the recent implications of TMEM16F CaPLSase in vascular development and endothelial cell-mediated coagulation, its signaling role in endothelial biology remains to be established. Here, we show that endothelial TMEM16F regulates in vitro and in vivo angiogenesis through intracellular signaling. Developmental retinal angiogenesis is significantly impaired in TMEM16F deficient mice, as evidenced by fewer vascular loops and larger loop areas. Consistent with our in vivo observation, TMEM16F siRNA knockdown in human umbilical vein endothelial cells compromises angiogenesis in vitro. We further discovered that TMEM16F knockdown enhances VE-cadherin phosphorylation and reduces its expression. Moreover, TMEM16F knockdown also promotes Src kinase phosphorylation at tyrosine 416, which may be responsible for downregulating VE-cadherin expression. Our study thus uncovers a new biological function of TMEM16F in angiogenesis and provides a potential mechanism for how the CaPLSase regulates angiogenesis through intracellular signaling.

3.
Elife ; 112022 06 07.
Artículo en Inglés | MEDLINE | ID: mdl-35670667

RESUMEN

TMEM16F, a Ca2+-activated phospholipid scramblase (CaPLSase), is critical for placental trophoblast syncytialization, HIV infection, and SARS-CoV2-mediated syncytialization, however, how TMEM16F is activated during cell fusion is unclear. Here, using trophoblasts as a model for cell fusion, we demonstrate that Ca2+ influx through the Ca2+ permeable transient receptor potential vanilloid channel TRPV4 is critical for TMEM16F activation and plays a role in subsequent human trophoblast fusion. GSK1016790A, a TRPV4 specific agonist, robustly activates TMEM16F in trophoblasts. We also show that TRPV4 and TMEM16F are functionally coupled within Ca2+ microdomains in a human trophoblast cell line using patch-clamp electrophysiology. Pharmacological inhibition or gene silencing of TRPV4 hinders TMEM16F activation and subsequent trophoblast syncytialization. Our study uncovers the functional expression of TRPV4 and one of the physiological activation mechanisms of TMEM16F in human trophoblasts, thus providing us with novel strategies to regulate CaPLSase activity as a critical checkpoint of physiologically and disease-relevant cell fusion events.


Asunto(s)
Anoctaminas/metabolismo , COVID-19 , Infecciones por VIH , Proteínas de Transferencia de Fosfolípidos/metabolismo , Calcio/metabolismo , Femenino , Humanos , Placenta/metabolismo , Embarazo , ARN Viral , SARS-CoV-2 , Canales Catiónicos TRPV/genética , Canales Catiónicos TRPV/metabolismo , Trofoblastos/metabolismo
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