[Treatment of severe hepatic insufficiency by perfusion of patients' blood through a suspension of cryopreserved hepatocytes]. / Lechenie tiazheloi pechenochnoi nedostatochnosti perfuziei krovi bol'nogo cherez vzves' kriokonservirovannykh gepatotsitov.

The results of hemoperfusion through a suspension of cryoconserved hepatocytes in 21 patients with severe hepatic insufficiency are analysed. It is shown that the manipulation had a beneficial effect on the clinical and biochemical indices, which was manifested by diminished jaundice and skin pruritus, increased physical activity, improved appetite; reduced levels of bilirubin, transaminase, alkaline phosphatase, bile acids, and cholesterol. The information gained is evidence that the method can be included in the complex treatment of patients with severe hepatic insufficiency.

[Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin]. / Poluchenie suspenzii izolirovannykh gepatotsitov s pomoshch'iu otechestvennogo preparata kollalitina.

Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.

[Dynamic activity of the splenic lymphocytes during the modelling of chronic denervation-delymphatization of the kidneys in mice]. / Dinamika aktivnosti limfotsitov selezenki pri modelirovanii khronicheskoi denervatsii--delimfatizatsii pochek u myshei.

In experiments on mice CBA the activity of spleen lymphocytes was investigated in sham-operated mice and in mice with denervation-delymphatization of the both kidneys. The activity of lymphocytes was determined by registration of amounts of colony-formed elements by the method of Till and McCulloch. Periodic increase of the lymphocytes activity has been observed in mice with denervation-delymphatization kidneys. It is supposed that the periodic activation of lymphoid system, induced by denervation-delymphatization, may be one of triggering mechanisms of transplant rejection.

[Tissue enzymatic study in the perfusate of cadaveric kidneys for assessing their functional state]. / Izuchenie tkanevykh fermentov v perfuzate trupnykh pochek dlia otsenki ikh funktsional'nogo sostoianiia.

A correlation was found between the level of the tissue enzyme activity in cadaveric kidneys perfusates and the degree of their functional adequacy in the early posttransplantation period. According to the data obtained the level of the activity of leucin aminopeptidase and gamma-glutamyltranspeptidase in the perfusates may be of importance for prognostication of a delayed function of the kidneys after transplantation. An elevated level of activity, in addition to leucin adminopeptidase and gamma-glutamyltranspeptidase, lactatedehydrogenase, aspartataminotransferase, muramidase, acid and alkali phosphatases suggests the transplanted kidney to be not functioning.