RESUMEN
Limited information is available on methods to reduce concentrations of the opium alkaloid noscapine in poppy seeds. A series of experiment were conducted using poppy seeds to evaluate the impact of thermal treatments, water rinsing, and baking on noscapine concentrations. A sample set of commercially available poppy seeds (n=15) was screened for noscapine using liquid chromatography-tandem mass spectrometry. The mean and median noscapine concentrations for poppy seed samples above the limit of quantitation (LOQ) was 89.9 and 28.4 mg kg-1, respectively. Six out of 15 samples were less than the LOQ. Poppy seed samples containing a mean noscapine concentration of 121 mg kg-1 were subjected to dry heat treatments ranging from 120-200 °C and a 5 min rinse with water. Baking experiments were also done by incorporating the poppy seeds into a muffin batter and baking in an oven at 200 °C. The dry heat treatment experiments showed that noscapine degraded at 160-200 °C, with a 50% loss of noscapine observed after 3.44 ± 0.46 min at 200 °C. Although the mean concentration of noscapine decreased when a muffin containing poppy seeds was baked at 200 °C for 16 min, these changes were not statistically significant (P>0.05). Rinsing the poppy seeds with water did not have a significant effect on noscapine concentrations. Together, these data allow for better characterization of potential dietary exposure to noscapine and indicate that certain thermal treatments can be effective for reduction of noscapine in poppy seeds.
RESUMEN
Limited information exists on the effectiveness of potential treatments to reduce levels of opium alkaloids that may be present in seeds from poppy (Papaver somniferum L.). Poppy seeds containing morphine at relatively lower (14.7 mg kg-1) and higher (210.0 mg kg-1) concentrations were subjected to dry heat and steam treatments, water washing, and baking. Sample extracts were then analyzed using liquid chromatography-tandem mass spectrometry for the opium alkaloids morphine, codeine, and thebaine. The results indicated that thermal treatment promoted opium alkaloid degradation in poppy seed samples, with a 50% loss of morphine observed after 30-40 min at 200 °C. Water washing reduced concentrations of opium alkaloids in poppy seeds by approximately 50-80%, while steam treatment resulted in reduction of morphine in only one sample type. Importantly, baking had no significant effect on concentrations of opium alkaloids. Overall, these results indicate that opium alkaloids may not be significantly affected by baking or steam application and that poppy seeds may require water washing or extended thermal treatment to promote reduction of these compounds.
