RESUMEN
The dual-focus vision observed in eagles' eyes is an intriguing phenomenon captivates scientists since a long time. Inspired by this natural occurrence, the authors' research introduces a novel bifocal meta-device incorporating a polarized camera capable of simultaneously capturing images for two different polarizations with slightly different focal distances. This innovative approach facilitates the concurrent acquisition of underfocused and overfocused images in a single snapshot, enabling the effective extraction of quantitative phase information from the object using the transport of intensity equation. Experimental demonstrations showcase the application of quantitative phase imaging to artificial objects and human embryonic kidney cells, particularly emphasizing the meta-device's relevance in dynamic scenarios such as laser-induced ablation in human embryonic kidney cells. Moreover, it provides a solution for the quantification during the dynamic process at the cellular level. Notably, the proposed eagle-eye inspired meta-device for phase imaging (EIMPI), due to its simplicity and compact nature, holds promise for significant applications in fields such as endoscopy and headsets, where a lightweight and compact setup is essential.
RESUMEN
Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.
Asunto(s)
Roturas del ADN/efectos de la radiación , Reparación del ADN , ADN/biosíntesis , Fase G1/genética , Mitosis/genética , Animales , Línea Celular , ADN/genética , ADN/efectos de la radiación , Fase G1/efectos de la radiación , Humanos , Rayos Infrarrojos/efectos adversos , Rayos Láser/efectos adversos , Mitosis/efectos de la radiación , PotoroidaeRESUMEN
Multicolored water-soluble fluorescent CdTe quantum dots (QDs) have been synthesized by using Te, NaBH(4), and CdCl(2) as precursors and several mercapto-based compounds as modifiers. The effects of ratio of the precursor, type of modifier, refluxing time and initial pH value of the reaction on fluorescence intensity of CdTe QDs have been studied. XRD, HRTEM and fluorescence spectra were employed to characterize these samples. It is found that CdTe QDs with a crystalline size of 3-4nm are homogeneously embedded in the organic matrix. The maximum fluorescence emission peaks of CdTe QDs solutions shifted from 525nm to 591nm according to the refluxing time. The fingerprint details on the surface of smooth objects were clearly developed with CdTe QDs solution under irradiation of UV lights.
