[Analysis of the stability of some properties of tick-borne encephalitis virus (strain 205) upon passaging in mice]. / Analiz stabil'nosti nekotorykh svoistv virusa kleshchevogo éntsefalita (shtamm 205) pri passirovanii na myshakh.

Comparative analysis of the characteristics of tick-borne encephalitis (TBE) strain 205 used for the production of vaccine against TBE and of its variants obtained by passages in mouse brain showed the stability of such properties as infective activity, neurovirulence, sensitivity to physical (heating to 50 C) and chemical (sodium deoxycholate treatment) factors. At the same time increased neurovirulence of variants 205/M10 and 205/M20, which undergone through 10 and 20 passages in white mouse brain, for low-sensitive Syrian hamsters was revealed. Use of a panel of 5 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein E and of 4 monoclonal antibodies to protein NS3 helped differentiate between not only strains 205 and Sofyin, but between variants of strain 205 as well.

[Epitope specificity and protective activity of monoclonal antibodies to Venezuelan equine encephalomyelitis virus]. / Epitopnaia spetsifichnost' i protektivnaia aktivnost' monoklonal'nykh antitel k virusu venesuél'skogo éntsefalomielita loshadei.

Epitope specificity and protective activity of 5 types of monoclonal antibodies (MAB) to strain TM-14 of Venezuelan equine encephalomyelitis (VEE) virus were studied. The competing VEE A4 and VEE B5 MAB were shown to recognize the conformation-dependent epitope of glycoprotein E2 third site and to possess an extremely high protective activity. VEE C6 MAB were active in all the studied biological tests (hemagglutination inhibition, neutralization tests, passive defence) and were directed to glycoprotein E2 site six. Competitive radioimmunoassay demonstrated that VEE A5 MAB also interacted with E2-6 site and by the sum of characteristics detected a new epitope of this site. VEE G1 MAB specific to glycoprotein E1 were characterized by protective, but not neutralizing activity, and competed with MAB to sites E2-2 and E2-6. This means that VEE G1 MAB detect a heretofore not described epitope E1 overlapping with two E2 sites.

[The biological and immunochemical properties of monoclonal antibodies to the Venezuelan equine encephalomyelitis virus]. / Biologicheskie i immunokhmicheskie svoistva monoklonal'nykh antitel k virusu venesuél'skogo éntsefalomielita loshadei.

Five strains of hybrid cells producing highly active monoclonal antibodies (MCA) to Venezuelan equine encephalomyelitis (VEE) virus were generated. VEEC6 MCA had high virus-neutralizing and hemagglutinating activities attesting to their direction to the "critical" site of E2 glycoprotein. MCA VEEA5 directed to the same glycoprotein did not overlap spatially with the previous one. MCA VEEB5 and VEEA4 interacted with conformational virus determinants taking part in hemagglutination.

[Action of doxorubicin and a doxorubicin-heparin complex on the growth and metastasis of experimental tumors and the hemostatic system indices]. / Deistvie doksorubitsina i kompleksa doksorubitsin--geparin na rost i metastazirovanie éksperimental'nykh opukholei i nekotorye pokazateli sistemy gemostaza.

The electrophoretic and spectral analyses have been used to show the possibility to form a complex consisting of doxorubicin and adriamycin with heparin, the molar ratio being 6:1 and pH 4.8-7.4. Doxorubicin and adriamycin had procoagulant properties but the doxorubicin-heparin complex showed an anticoagulant activity. In experiments on rats with the Pliss lymphosarcoma and sarcoma 45 the doxorubicin-heparin complex depressed more efficiently the tumour growth and metastasis spreading. The combination of doxorubicin and the doxorubicin heparin complex with the trypsin-heparin complex which imitate the hyperfunction of anticoagulative system markedly increased the antitumour effects.