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1.
Basic Res Cardiol ; 115(6): 62, 2020 09 25.
Artículo en Inglés | MEDLINE | ID: mdl-32975669

RESUMEN

Neuraminidase (NEU)1 forms a multienzyme complex with beta-galactosidase (ß-GAL) and protective-protein/cathepsin (PPC) A, which cleaves sialic-acids from cell surface glycoconjugates. We investigated the role of NEU1 in the myocardium after ischemia/reperfusion (I/R). Three days after inducing I/R, left ventricles (LV) of male mice (3 months-old) displayed upregulated neuraminidase activity and increased NEU1, ß-GAL and PPCA expression. Mice hypomorphic for neu1 (hNEU1) had less neuraminidase activity, fewer pro-inflammatory (Lin-CD11b+F4/80+Ly-6Chigh), and more anti-inflammatory macrophages (Lin-CD11b+F4/80+Ly-6Clow) 3 days after I/R, and less LV dysfunction 14 days after I/R. WT mice transplanted with hNEU1-bone marrow (BM) and hNEU1 mice with WT-BM showed significantly better LV function 14 days after I/R compared with WT mice with WT-BM. Mice with a cardiomyocyte-specific NEU1 overexpression displayed no difference in inflammation 3 days after I/R, but showed increased cardiomyocyte hypertrophy, reduced expression and mislocalization of Connexin-43 in gap junctions, and LV dysfunction despite a similar infarct scar size to WT mice 14 days after I/R. The upregulation of NEU1 after I/R contributes to heart failure by promoting inflammation in invading monocytes/macrophages, enhancing cardiomyocyte hypertrophy, and impairing gap junction function, suggesting that systemic NEU1 inhibition may reduce heart failure after I/R.


Asunto(s)
Insuficiencia Cardíaca/etiología , Hipertrofia Ventricular Izquierda/etiología , Macrófagos/enzimología , Monocitos/enzimología , Infarto del Miocardio/complicaciones , Daño por Reperfusión Miocárdica/complicaciones , Miocitos Cardíacos/enzimología , Neuraminidasa/deficiencia , Disfunción Ventricular Izquierda/etiología , Animales , Catepsina A/metabolismo , Conexina 43/metabolismo , Modelos Animales de Enfermedad , Femenino , Uniones Comunicantes/enzimología , Uniones Comunicantes/patología , Insuficiencia Cardíaca/enzimología , Insuficiencia Cardíaca/inmunología , Insuficiencia Cardíaca/fisiopatología , Hipertrofia Ventricular Izquierda/enzimología , Hipertrofia Ventricular Izquierda/inmunología , Hipertrofia Ventricular Izquierda/fisiopatología , Macrófagos/inmunología , Masculino , Ratones de la Cepa 129 , Ratones Endogámicos C57BL , Ratones Transgénicos , Monocitos/inmunología , Infarto del Miocardio/enzimología , Infarto del Miocardio/inmunología , Infarto del Miocardio/patología , Daño por Reperfusión Miocárdica/enzimología , Daño por Reperfusión Miocárdica/inmunología , Daño por Reperfusión Miocárdica/patología , Miocitos Cardíacos/patología , Neuraminidasa/genética , Disfunción Ventricular Izquierda/enzimología , Disfunción Ventricular Izquierda/inmunología , Disfunción Ventricular Izquierda/fisiopatología , Función Ventricular Izquierda , Remodelación Ventricular , beta-Galactosidasa/metabolismo
2.
J Psychiatr Res ; 105: 95-102, 2018 10.
Artículo en Inglés | MEDLINE | ID: mdl-30216787

RESUMEN

Schizophrenia and major depression are associated with alterations in peripheral inflammatory markers, and anti-inflammatory therapy has been proposed as a promising add-on approach in the pharmacologic treatment of both disorders. Second-generation atypical antipsychotics are currently first-line drugs in the treatment of schizophrenia and are also used as augmentation strategies in treatment-resistant major depression. Furthermore, these drugs have been reported to exhibit distinct metabolic side effects and to influence inflammatory processes. In this study, we used ex vivo stimulation of primary human peripheral blood mononuclear cells (PBMC) from healthy blood donors with atypical antipsychotics olanzapine or aripiprazole to examine effects on cytokine production independent from metabolic side effects and disease status. Both olanzapine and aripiprazole stimulation decreased mRNA levels of IL-1ß, IL-6, and TNF-α and resulted in diminished protein concentrations of IL-6 and TNF-α in conditioned medium of stimulated PBMC. A multiplex approach revealed additional downregulation of IL-2; MIP-1ß and IP-10 secretion. Similarly, olanzapine and aripiprazole stimulation of the human monocytic cell line THP-1 resulted in a significant decrease in expression and secretion of IL-1ß and TNF-α. Our results suggest that atypical antipsychotics directly influence immune cell function and thereby highlight the importance to factor in potential side effects of drugs routinely used in treatment of schizophrenia and major depression on inflammatory processes when considering anti-inflammatory drug therapy as an additional treatment option.


Asunto(s)
Antipsicóticos/farmacología , Aripiprazol/farmacología , Citocinas/efectos de los fármacos , Inflamación , Leucocitos Mononucleares/efectos de los fármacos , Olanzapina/farmacología , Humanos , Células THP-1
3.
Vascul Pharmacol ; 103-105: 16-28, 2018 04.
Artículo en Inglés | MEDLINE | ID: mdl-29371126

RESUMEN

Inflammation plays an important role in atherosclerosis, a notion supported by the beneficial effects of the IL-1ß inhibitor canakinumab in the CANTOS trial. Sialic acids (Sias), components of the surface glycocalyx, regulate intercellular and intermolecular interactions. We investigated the expression of the Sia cleaving enzyme neuraminidase-1 (NEU1) in atherosclerotic plaques and its potential role in inflammatory processes. In isolated mononuclear blood cells from patients with myocardial infarction, NEU1 expression was increased compared to healthy controls. High expression of NEU1 in macrophages located on the intima layer, in calcified regions and the adventitia of the plaque was observed in human carotid arteries' atherectomies. IL-1ß and LPS induced NEU1 expression in THP-1 monocytic cells. Lentiviral NEU1-overexpression in THP-1-cells enhanced expression of CD80, TNF-α, IL-1ß, number of multinuclear cells, phagocytosis and chemotaxis indicative for M1 monocyte/macrophage polarization. CRISPR/Cas9-mediated knock-out of NEU1 in THP-1-cells did not affect differentiation of monocytes to macrophages but attenuated LPS- and IL-1ß -induced TNF-α and IL-1ß expression. SiRNA-mediated knock-down of NEU1 in M1-macrophages differentiated from primary human CD14+ monocytes reduced the expression of TNF-α and IL-1ß. Thus, in monocytes/macrophages, LPS, NEU1 and IL-1ß act in a positive feedback loop as enhancers of inflammation and may therefore promote atherosclerosis and plaque instability.


Asunto(s)
Aterosclerosis/patología , Inflamación/patología , Neuraminidasa/genética , Placa Aterosclerótica/patología , Aterosclerosis/genética , Arterias Carótidas/patología , Estudios de Casos y Controles , Diferenciación Celular/genética , Técnicas de Silenciamiento del Gen , Humanos , Inflamación/genética , Interleucina-1beta/metabolismo , Leucocitos Mononucleares/patología , Lipopolisacáridos/toxicidad , Macrófagos/patología , Monocitos/patología , Infarto del Miocardio/patología , Placa Aterosclerótica/genética
4.
Vascul Pharmacol ; 100: 26-33, 2018 01.
Artículo en Inglés | MEDLINE | ID: mdl-28919014

RESUMEN

In the vascular system, the endothelial surface layer (ESL) as the inner surface of blood vessels affects mechanotransduction, vascular permeability, rheology, thrombogenesis, and leukocyte adhesion. It creates barriers between endothelial cells and blood and neighbouring cells. The glycocalyx, composed of glycoconjugates and proteoglycans, is an integral component of the ESL and a key element in inter- and intracellular communication and tissue homeostasis. In pathophysiological conditions (atherosclerosis, infection, ischemia/reperfusion injury, diabetes, trauma and acute lung injury) glycocalyx-degrading factors, i.e. reactive oxygen and nitrogen species, matrix metalloproteinases, heparanase and sialidases, damage the ESL, thereby impairing endothelial functions. This leads to increased capillary permeability, leucocyte-endothelium interactions, thrombosis and vascular inflammation, the latter further driving glycocalyx destruction. The present review highlights current knowledge on the vasculoprotective role of the ESL, with specific emphasis on its remodelling in inflammatory vascular diseases and discusses its potential as a novel therapeutic target to treat vascular pathologies.


Asunto(s)
Células Endoteliales/metabolismo , Glicocálix/metabolismo , Inflamación/metabolismo , Enfermedades Vasculares/metabolismo , Remodelación Vascular , Animales , Antiinflamatorios/uso terapéutico , Fármacos Cardiovasculares/uso terapéutico , Células Endoteliales/efectos de los fármacos , Células Endoteliales/patología , Glicocálix/efectos de los fármacos , Glicocálix/patología , Humanos , Inflamación/tratamiento farmacológico , Inflamación/patología , Inflamación/fisiopatología , Mediadores de Inflamación/metabolismo , Transducción de Señal , Enfermedades Vasculares/tratamiento farmacológico , Enfermedades Vasculares/patología , Enfermedades Vasculares/fisiopatología , Remodelación Vascular/efectos de los fármacos
5.
Eur Heart J ; 38(5): 349-361, 2017 02 01.
Artículo en Inglés | MEDLINE | ID: mdl-28201733

RESUMEN

Aims: The benefit of the ß1-adrenergic receptor (ß1-AR) agonist dobutamine for treatment of acute heart failure in peripartum cardiomyopathy (PPCM) is controversial. Cardiac STAT3 expression is reduced in PPCM patients. Mice carrying a cardiomyocyte-restricted deletion of STAT3 (CKO) develop PPCM. We hypothesized that STAT3-dependent signalling networks may influence the response to ß-AR agonist treatment in PPCM patients and analysed this hypothesis in CKO mice. Methods and Results: Follow-up analyses in 27 patients with severe PPCM (left ventricular ejection fraction ≤25%) revealed that 19 of 20 patients not obtaining dobutamine improved cardiac function. All seven patients obtaining dobutamine received heart transplantation (n = 4) or left ventricular assist devices (n = 3). They displayed diminished myocardial triglyceride, pyruvate, and lactate content compared with non-failing controls. The ß-AR agonist isoproterenol (Iso) induced heart failure with high mortality in postpartum female, in non-pregnant female and in male CKO, but not in wild-type mice. Iso induced heart failure and high mortality in CKO mice by impairing fatty acid and glucose uptake, thereby generating a metabolic deficit. The latter was governed by disturbed STAT3-dependent signalling networks, microRNA-199a-5p, microRNA-7a-5p, insulin/glucose transporter-4, and neuregulin/ErbB signalling. The resulting cardiac energy depletion and oxidative stress promoted dysfunction and cardiomyocyte loss inducing irreversible heart failure, which could be attenuated by the ß1-AR blocker metoprolol or glucose-uptake-promoting drugs perhexiline and etomoxir. Conclusions: Iso impairs glucose uptake, induces energy depletion, oxidative stress, dysfunction, and death in STAT3-deficient cardiomyocytes mainly via ß1-AR stimulation. These cellular alterations may underlie the dobutamine-induced irreversible heart failure progression in PPCM patients who frequently display reduced cardiac STAT3 expression.


Asunto(s)
Agonistas de Receptores Adrenérgicos beta 1/efectos adversos , Agonistas de Receptores Adrenérgicos beta 1/toxicidad , Cardiomiopatías/inducido químicamente , Dobutamina/efectos adversos , Insuficiencia Cardíaca/tratamiento farmacológico , Trastornos Puerperales/tratamiento farmacológico , Factor de Transcripción STAT3/fisiología , Adulto , Animales , Glucemia/metabolismo , Femenino , Humanos , Isoproterenol/farmacología , Masculino , Ratones Noqueados , MicroARNs/fisiología , Mitocondrias Cardíacas/metabolismo , Miocardio/metabolismo , Miocitos Cardíacos/metabolismo , Periodo Periparto , Nucleótidos de Purina/metabolismo , Distribución Aleatoria , Especies Reactivas de Oxígeno/metabolismo , Receptor ErbB-4/metabolismo , Factor de Transcripción STAT3/antagonistas & inhibidores , Factor de Transcripción STAT3/deficiencia , Disfunción Ventricular Izquierda/inducido químicamente
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