Cytogenetic analysis of nuclear abnormalities in the erythrocytes of gecko lizards (Phyllopezus periosus) collected in a semi-arid region of northeast Brazil: Possible effects of natural background radioactivity.

High natural-background radioactivity levels occur in the semi-arid region of the State of Rio Grande do Norte, northeastern Brazil. We have studied the lizard Phyllopezus periosus, an endemic species of the Brazilian caatinga with saxicolous habitat, as a bioindicator of environmental quality. Specimens were collected in three areas, an environmental protection area and two areas recognized as having high natural background radiation, one of these being a mining area. Level of metals and gamma radiation emitters present in the water sources potentially used by the lizards were measured. The biological endpoints assessed were micronuclei and nuclear abnormalities in blood samples. Significant differences in background radioactivity levels were found among the assessed areas. Statistically significant differences in micronuclei and nuclear abnormality frequencies were seen, among the study areas and a relationship between radioactivity level and genetic damage was observed.

Lizards as model organisms to evaluate environmental contamination and biomonitoring.

Environmental contamination is reaching ever higher levels and affecting several animal populations, including humans. In this context, studies are being developed to monitor and evaluate this environmental problem using bioindicators organisms, in addition to testing the toxicity of contaminants in the laboratory. In this perspective, reptiles are ideal animals for these types of studies, considering that they are ectothermic and have a slower metabolism directly influencing their recovery power, and therefore, they are more sensitive to xenobiotic effects. Among reptiles, lizards are animals that adapt to various environmental conditions, even being found in areas with arid characteristics. Therefore, a literature review was conducted in this study regarding the use of lizards as models for ecotoxicological studies, including biomonitoring, carried out in the last 10 years, with the aim of evaluating them as bioindicators in Brazilian semi-arid region. Studies were found involving ten lizard families, among which the most investigated was Lacertidae. The studies were classified into two categories: organic contaminants (pesticides, petroleum by-products, and explosives) and inorganic contaminants (metals such as zinc, lead and aluminum, and radionuclides). Contaminants directly contributed to DNA damage and to increasing the frequency of micronuclei in exposed animals, histopathological effects, and oxidative stress. The performed analysis highlights the usefulness of lizards as environmental biomonitors. However, the response profile is dependent on the exposure level and route, in addition to the environmental scenario analyzed. Therefore, future studies aimed at evaluating environmental contaminants are required under exposure conditions more related to the environmental reality to be studied.