Exploring biodegradative efficiency: a systematic review on the main microplastic-degrading bacteria.

Introduction: Microplastics (MPs) are widely distributed in the environment, causing damage to biota and human health. Due to their physicochemical characteristics, they become resistant particles to environmental degradation, leading to their accumulation in large quantities in the terrestrial ecosystem. Thus, there is an urgent need for measures to mitigate such pollution, with biological degradation being a viable alternative, where bacteria play a crucial role, demonstrating high efficiency in degrading various types of MPs. Therefore, the study aimed to identify bacteria with the potential for MP biodegradation and the enzymes produced during the process. Methods: The methodology used followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) protocol. Results and Discussion: The research yielded 68 eligible studies, highlighting bacteria from the genera Bacillus, Pseudomonas, Stenotrophomonas, and Rhodococcus as the main organisms involved in MP biodegradation. Additionally, enzymes such as hydrolases and alkane hydroxylases were emphasized for their involvement in this process. Thus, the potential of bacterial biodegradation is emphasized as a promising pathway to mitigate the environmental impact of MPs, highlighting the relevance of identifying bacteria with biotechnological potential for large-scale applications in reducing MP pollution.

Plant Essential Oils as Biopesticides: Applications, Mechanisms, Innovations, and Constraints.

The advent of the "Green Revolution" was a great success in significantly increasing crop productivity. However, it involved high ecological costs in terms of excessive use of synthetic agrochemicals, raising concerns about agricultural sustainability. Indiscriminate use of synthetic pesticides resulted in environmental degradation, the development of pest resistance, and possible dangers to a variety of nontarget species (including plants, animals, and humans). Thus, a sustainable approach necessitates the exploration of viable ecofriendly alternatives. Plant-based biopesticides are attracting considerable attention in this context due to their target specificity, ecofriendliness, biodegradability, and safety for humans and other life forms. Among all the relevant biopesticides, plant essential oils (PEOs) or their active components are being widely explored against weeds, pests, and microorganisms. This review aims to collate the information related to the expansion and advancement in research and technology on the applications of PEOs as biopesticides. An insight into the mechanism of action of PEO-based bioherbicides, bioinsecticides, and biofungicides is also provided. With the aid of bibliometric analysis, it was found that ~75% of the documents on PEOs having biopesticidal potential were published in the last five years, with an annual growth rate of 20.51% and a citation per document of 20.91. Research on the biopesticidal properties of PEOs is receiving adequate attention from European (Italy and Spain), Asian (China, India, Iran, and Saudi Arabia), and American (Argentina, Brazil, and the United States of America) nations. Despite the increasing biopesticidal applications of PEOs and their widespread acceptance by governments, they face many challenges due to their inherent nature (lipophilicity and high volatility), production costs, and manufacturing constraints. To overcome these limitations, the incorporation of emerging innovations like the nanoencapsulation of PEOs, bioinformatics, and RNA-Seq in biopesticide development has been proposed. With these novel technological interventions, PEO-based biopesticides have the potential to be used for sustainable pest management in the future.

Halophytes for the sustainable remediation of heavy metal-contaminated sites: Recent developments and future perspectives.

Increasing land degradation by high level of metal wastes is of prime concern for the global research communities. In this respect, halophytes having specific features like salt glands, exclusion of excess ions, heavy metals (HMs) compartmentalization, large pool of antioxidants, and associations with metal-tolerant microbes are of great promise in the sustainable clean-up of contaminated sites. However, sustainable clean-up of HMs by a particular halophyte plant species is governed considerably by physico-chemical characteristics of soil and associated microbial communities. The present review has shed light on the superiority of halophytes over non-halophytes, mechanisms of metal-remediation, recent developments and future perspectives pertaining to the utilization of halophytes in management of HM-contaminated sites with the aid of bibliometric analysis. The results revealed that the research field is receiving considerable attention in the last 5-10 years by publishing ∼50-90% documents with an annual growth rate of 15.41% and citations per document of 29.72. Asian (viz., China, India, and Pakistan) and European (viz., Spain, Portugal, Belgium, Argentina) countries have been emerged as the major regions conducting and publishing extensive research on this topic. The investigations conducted both under in vitro and field conditions have reflected the inherent potential of halophyte as sustainable research tool for successfully restoring the HM-contaminated sites. The findings revealed that the microbial association with halophytes under different challenging conditions is a win-win approach for metal remediation. Therefore, exploration of new halophyte species and associated microorganisms (endophytic and rhizospheric) from different geographical locations, and identification of genes conferring tolerance and phytoremediation of metal contaminants would further advance the intervention of halophytes for sustainable ecological restoration.

Pesticide contamination in agro-ecosystems: toxicity, impacts, and bio-based management strategies.

Continuous rise in application of pesticides in the agro-ecosystems in order to ensure food supply to the ever-growing population is of greater concern to the human health and the environment. Once entered into the agro-ecosystem, the fate and transport of pesticides is determined largely by the nature of pesticides and the soil attributes, in addition to the soil-inhabiting microbes, fauna, and flora. Changes in the soil microbiological actions, soil properties, and enzymatic activities resulting from pesticide applications are the important factors substantially affecting the soil productivity. Disturbances in the microbial community composition may lead to the considerable perturbations in cycling of major nutrients, metals, and subsequent uptake by plants. Indiscriminate applications are linked with the accumulation of pesticides in plant-based foods, feeds, and animal products. Furthermore, rapid increase in the application of pesticides having long half-life has also been reported to contaminate the nearby aquatic environments and accumulation in the plants, animals, and microbes surviving there. To circumvent the negative consequences of pesticide application, multitude of techniques falling in physical, chemical, and biological categories are presented by different investigators. In the present study, important findings pertaining to the pesticide contamination in cultivated agricultural soils; toxicity on soil microbes, plants, invertebrates, and vertebrates; effects on soil characteristics; and alleviation of toxicity by bio-based management approaches have been thoroughly reviewed. With the help of bibliometric analysis, thematic evolution and research trends on the bioremediation of pesticides in the agro-ecosystems have also been highlighted.

Projected Impacts of Climate Change on the Range Expansion of the Invasive Straggler Daisy (Calyptocarpus vialis) in the Northwestern Indian Himalayan Region.

Human-induced climate change modifies plant species distribution, reorganizing ecologically suitable habitats for invasive species. In this study, we identified the environmental factors that are important for the spread of Calyptocarpus vialis, an emerging invasive weed in the northwestern Indian Himalayan Region (IHR), along with possible habitats of the weed under current climatic scenarios and potential range expansion under several representative concentration pathways (RCPs) using MaxEnt niche modeling. The prediction had a high AUC (area under the curve) value of 0.894 ± 0.010 and a remarkable correlation between the test and expected omission rates. BIO15 (precipitation seasonality; 38.8%) and BIO1 (annual mean temperature; 35.7%) had the greatest impact on the probable distribution of C. vialis, followed by elevation (11.7%) and landcover (6.3%). The findings show that, unlike the current situation, "high" and "very high" suitability areas would rise while less-suited habitats would disappear. All RCPs (2.6, 4.5, 6.0, and 8.5) indicate the expansion of C. vialis in "high" suitability areas, but RCP 4.5 predicts contraction, and RCPs 2.6, 6.0, and 8.5 predict expansion in "very high" probability areas. The current distribution of C. vialis is 21.59% of the total area of the state, with "medium" to "high" invasion suitability, but under the RCP 8.5 scenario, it might grow by 10% by 2070. The study also reveals that C. vialis may expand its niche at both lower and higher elevations. This study clarifies how bioclimatic and topographic factors affect the dispersion of invasive species in the biodiverse IHR. Policymakers and land-use managers can utilize the data to monitor C. vialis hotspots and develop scientifically sound management methods.

Effect of rainfall variability on tree phenology in moist tropical deciduous forests.

Plants in their life cycle go through a series of life processes. These phenological changes are influenced by different climatic conditions. Abiotic factors like temperature, precipitation, and photoperiodism affect the onset and offset of particular phenophase in the plant periodic cycle. In this study, we tested the influence of precipitation on the forest phenology at two sites of Dudhwa National Park (DNP), Uttar Pradesh and Simlipal National Park (SNP), Odisha, India. DNP and SNP receive an annual average rainfall of 1093.5 mm and 1500 mm, respectively, of which most rainfall (~ 90%) occurs during June-September. Normalized Difference Vegetation Index (NDVI) was measured for 2 years 2015 and 2018, with 2015 being a drought year and 2018 being a normal rainfall year. NDVI was analyzed at different temporal scales of months, season, and years using the t test (Welch's two-tailed) and General Linear Mixed Model (GLMM). Effect of drought (2015) and normal (2018) rainfall year was not significant at both the sites, whereas season, year*season interaction, season*rainfall interaction, and year*season*rainfall interaction were found significant at DNP (P < 0.05, ICC = 0.68, marginal R2 = 0.81; conditional R2 = 0.94). At SNP, rainfall, year, season, and their interaction were non-significant, whereas several months showed a significant effect on the NDVI values for both sites. Winter and monsoon season in DNP, and post-monsoon season in SNP, showed a significant effect on the NDVI patterns. Thus, the effect of precipitation stress in the deciduous forests was evident at small intervals of observation. Tree phenology compensated for differences when observed from a higher temporal scale of a year. There existed a mechanism in trees to tide over adverse conditions and maintain the phenology over longer intervals of time. The resilience and vulnerability of such forest ecosystems against abiotic factors and extreme events would be instrumental in climate change adaptation strategies. Tree phenology can be used as an indicator of forest health and resilience.

A global review of rubber plantations: Impacts on ecosystem functions, mitigations, future directions, and policies for sustainable cultivation.

The growing global need for latex is driving rubber plantation (RP) expansion since the last century, with >2 Mha of cultivation area being established in the last decade. Southeast Asia is the hotspot for rubber cultivation at other land-use costs. Although rubber cultivation has improved the economic status of farmers, it has altered the habitat's ecology and ecosystem functions (EF). However, studies on the impacts of RP on EF are limited, and a clear overview is not available. To bridge this gap, we conducted an inclusive review of the EF of RP, including soil carbon storage, aboveground biomass (AGB) and belowground biomass (BGB), litter production and decomposition, respiration, and biodiversity (plants, animals, soil fauna, and microbes). We compared the EF in RP (monoculture) with those in forests because the conversion of forests to RP is prevalent in the tropics and because most RP studies used forests as reference ecosystems. We found RP generally have lower EF than forests. The impacts of RP on some EF are more severe (e.g., AGB, BGB, and plant diversity), causing decreases of >55%, and the effects are consistently negative irrespective of plantation age. However, including agroforestry or polyculture, integrated pest management, cover cropping, mulching, and composting can improve the EF in RP to some extent. We highlighted research gaps, particularly substantial research gaps concerning the influence of plant diversity treatments (i.e., agroforestry) performed in RP on EF. Additionally, more empirical data on the significance of spatial and temporal levels are required, such as how the impact on EF could vary with climate and RP age, as we showed some examples where EF differs spatially and temporally. More importantly, further research on plantation management to offset EF losses is needed. Finally, we emphasized knowledge gaps and suggested future directions and policies for improving EF in RP.

Spatio-temporal variability in soil CO2 efflux and regulatory physicochemical parameters from the tropical urban natural and anthropogenic land use classes.

Urban ecosystems, the heterogeneous and rapidly changing landscape, showed a considerable impact on the global C cycle. However, studies encompassing the spatial differences in urban land uses on soil C dynamics are limited in tropical ecosystems. In this study, seasonal and temporal variability in soil CO2 efflux (SCE) and its regulatory physicochemical variables under five urban land use classes viz., Bare (BAR), Agriculture (AGR), Plantation (PLT), Grassland (GRA) and Lawns (LAW) were assessed from 2014 to 2016. Bare land use was considered as the reference for observing the variation for different land uses. Seasonal measurements of SCE, soil temperature, moisture content, pH, ammonium-N, nitrate-N and microbial biomass C (MBC) were performed whereas soil organic C (SOC), soil N, and soil physical properties were measured annually. Our results showed a significant (P < 0.01) increase in SCE by 89%, 117%, 132% and 166% for land use types from BAR to AGR, PLT, GRA and LAW, respectively. The results revealed a two-fold increase in SCE from anthropogenically managed urban lawns as compared to bare soil. PLT and LAW land use classes showed higher SOC and N contents. SCE was found positively correlated with temperature, moisture, SOC, soil N and MBC whereas negatively correlated with ammonium-N and nitrate-N (at P < 0.05) for the overall dataset. Soil moisture, temperature, SOC, porosity and pH were identified as the major determinant of urban SCE by explaining 63% of the variability in overall SCE. Further, temperature for BAR and LAW; moisture for PLT; ammonium-N for GRA; and nitrate-N for AGR were identified as the major regulators of SCE for different land use classes. The findings revealed that the interaction of soil temperature and moisture with nutrient availability regulates overall and seasonal variability in SCE in an urban ecosystem. Since these variables are highly affected by climate change, thus, the soil C source-sink relationships in tropical urban ecosystems may further change and induce a positive global warming potential from urban ecosystems.

Exploring temple floral refuse for biochar production as a closed loop perspective for environmental management.

Religious faith and ritual activities lead to significant floral offerings production and its disposal as waste to the nearby open lands and water bodies. These activities result into various social and environmental nuisances because of their high organic content. Alternatively, it can be used as valuable resources for various biochemical and thermo-chemical processes. Floral refuse has been utilized in natural dye extraction, however, the residual solid refuse is of significant environmental concern due to its nutrient rich nature. This study explores the potential utilization of solid residue of temple floral refuse after natural dye extraction by thermo-chemical decomposition of it. The slow pyrolysis of solid residue was performed at 350 °C and 500 °C, and the biochar yield of 42 and 36% was obtained, respectively. TGA-DTG analysis was performed to observe the thermo-chemical behaviour of floral refuse. The biochar products were further characterized by FTIR, SEM, EDX, BET, XRD, and RAMAN spectroscopy to observe the impact of pyrolysis temperature (PT) on the resulting material, i.e. biochar and its possible application measures. EDX results revealed the presence of various macro-nutrients such as C, N, P, K Ca and Mg in different proportions which showed its soil amelioration potential. Moreover, based on the SEM and BET results, biochar prepared at 500 °C was further explored for adsorption of methylene blue dye at various dose and pH conditions. Based on Langmuir (R2 = 0.98) and Freundlich (R2 = 0.97) isotherms, it is found as a potential adsorbent material for removal of methylene blue dye. The results revealed that biochar conversion of colour extracted floral refuse can be a vital option for quick and efficient management of it in a closed loop approach.

Emerging trends in photodegradation of petrochemical wastes: a review.

Various human activities like mining and extraction of mineral oils have been used for the modernization of society and well-beings. However, the by-products such as petrochemical wastes generated from such industries are carcinogenic and toxic, which had increased environmental pollution and risks to human health several folds. Various methods such as physical, chemical and biological methods have been used to degrade these pollutants from wastewater. Advance oxidation processes (AOPs) are evolving techniques for efficient sequestration of chemically stable and less biodegradable organic pollutants. In the present review, photocatalytic degradation of petrochemical wastes containing monoaromatic and poly-aromatic hydrocarbons has been studied using various heterogeneous photocatalysts (such as TiO2, ZnO and CdS. The present article seeks to offer a scientific and technical overview of the current trend in the use of the photocatalyst for remediation and degradation of petrochemical waste depending upon the recent advances in photodegradation of petrochemical research using bibliometric analysis. We further outlined the effect of various heterogeneous catalysts and their ecotoxicity, various degradation pathways of petrochemical wastes, the key regulatory parameters and the reactors used. A critical analysis of the available literature revealed that TiO2 is widely reported in the degradation processes along with other semiconductors/nanomaterials in visible and UV light irradiation. Further, various degradation studies have been carried out at laboratory scale in the presence of UV light. However, further elaborative research is needed for successful application of the laboratory scale techniques to pilot-scale operation and to develop environmental friendly catalysts which support the sustainable treatment technology with the "zero concept" of industrial wastewater. Nevertheless, there is a need to develop more effective methods which consume less energy and are more efficient in pilot scale for the demineralization of pollutant.

Assessment of ground and surface water quality along the river Varuna, Varanasi, India.

Multivariate statistical techniques were employed for monitoring of ground-surface water interactions in rivers. The river Varuna is situated in the Indo-Gangetic plain and is a small tributary of river Ganga. The study area was monitored at seven sampling sites for 3 years (2010-12), and eight physio-chemical parameters were taken into account for this study. The data obtained were analysed by multivariate statistical techniques so as to reveal the underlying implicit information regarding proposed interactions for the relevant area. The principal component analysis (PCA) and cluster analysis (CA), and the results of correlations were also studied for all parameters monitored at every site. Methods used in this study are essentially multivariate statistical in nature and facilitate the interpretation of data so as to extract meaningful information from the datasets. The PCA technique was able to compress the data from eight to three parameters and captured about 78.5% of the total variance by performing varimax rotation over the principal components. The varifactors, as yielded from PCA, were treated by CA which grouped them convincingly into three groups having similar characteristics and source of contamination. Moreover, the loading of variables on significant PCs showed correlations between various ground water and surface water (GW-SW) parameters. The correlation coefficients calculated for various physiochemical parameters for ground and surface water established the correlations between them. Thus, this study presents the utility of multivariate statistical techniques for evaluation of the proposed interactions and effective future monitoring of potential sites.