Neurotrophins and Trk receptors in the developing and adult ovary of Coturnix coturnix japonica.

NGF, BDNF, NT-3 and their specific receptors TrkA, TrkB and TrkC are known to be involved in the development and maintenance of vertebrates' nervous system. However, these molecules play a role also in non-neuronal tissue, such as in the reproductive system. In this study we investigated the presence and localization of neurotrophins and Trk receptors to unravel their potential role in the developing and adult ovary of Japanese quail, a model species well suited for reproduction studies. Western blotting analysis on ovaries of three month old quails in the period of egg laying showed the presence of pro and mature forms of neurotrophins and splice variants of Trk receptors. Immunohistochemical investigation reported that in embryonic ovaries from the 9th day of incubation to the hatching NGF and NT-3 were observed in the cortical and medullar areas respectively, whereas Trk receptors were observed in both areas. In adult ovary, all NTs were detected in glandular stromal cells, NGF and NT-3 also in the nervous component. Regarding follicle components, NGF and BDNF were observed in oocytes and follicular cells. All TrK receptors were present in nervous components and only TrkA in glandular stromal cells. In follicles, TrkA was present in oocyte cytoplasm and TrkB in theca cells. The results suggest an involvement of the neurotrophin system in the quail ovary physiology, promoting the oocyte development and follicular organization in the embryo, as well as oocyte and follicular maturation in adults.

The first report of massive infestation with Lipoptena Cervi (Diptera: Hippoboscidae) in Roe Deer (Capreolus Capreolus) in Iasi county, N-E of Romania / O primeiro relatório de infestação maciça com Lipoptena Cervi (Diptera: Hippoboscidae) em Roe Deer (Capreolus capreolus) no condado de Iasi, NE da Romênia

Investigations of four roe deer corpses were carried out from May until October 2014, in the Veterinary Forensic Laboratory and in the Parasitic Diseases Clinic, in the Iasi Faculty of Veterinary Medicine. The roe deer were harvested by shooting during the trophy hunting season. The clinical examination of the shot specimens revealed the presence of a highly consistent number of extremely mobile apterous insects, spread on the face, head, neck, lateral body parts, abdominal regions, inguinal, perianal and, finally, all over the body. The corpses presented weakening, anemia and cutaneous modification conditions. Several dozen insects were prelevated in a glass recipient and preserved in 70º alcoholic solution in order to identify the ectoparasite species. The morphological characteristics included insects in the Diptera order, Hippoboscidae family, Lipoptena cervi species. These are highly hematophagous insects that by severe weakening are affecting the game health and trophy quality. Histological investigations of the skin revealed some inflammatory reactions caused by ectoparasite Lipoptena cervi. Lipoptena cervi was identified for the first time in Iasi County, Romania.(AU)

As investigações de quatro cadáveres de veados foram realizadas desde maio até outubro de 2014, no Laboratório Forense de Veterinária e na Clínica de Doenças Parasitárias, da Faculdade de Medicina Veterinária de Iasi. Os veados foram abatidos por tiro durante a temporada de caça troféu. O exame clínico dos espécimes revelou a presença de um número muito consistente de insetos ápteros extremamente móveis, espalhados pela face, pela cabeça, pelo pescoço, pelas partes do corpo laterais, pelas regiões abdominal, inguinal, perianal e, finalmente, por todo o corpo. Os cadáveres apresentavam condições de enfraquecimento, anemia e alterações cutâneas. Várias dezenas de insetos foram separados em um recipiente de vidro e preservados em solução alcoólica de 70º, a fim de se identificarem as espécies de ectoparasitos. As características morfológicas incluem os insetos da ordem Diptera, família Hippoboscidae, espécie Lipoptena Cervi. Estes são insetos hematófagos que estão enfraquecendo e afetando a qualidade de saúde de animais de caça e troféu. Investigações histológicas da pele revelaram algumas reações inflamatórias causadas pelo ectoparasita cervi Lipoptena. Lipoptena cervi foi identificado pela primeira vez no condado de Iasi, na Romênia.(AU)

WITHDRAWN: Neurotrophins and specific receptors in the oviduct tracts of Japanese quail (Coturnix coturnix japonica).

The Publisher regrets that this article is an accidental duplication of an article that has already been published in Ann. Anat., 210 (2017) 112 ­ 120>, http://dx.doi.org/10.1016/j.aanat.2016.04.034. The duplicate article has therefore been withdrawn. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.

Neurotrophins and specific receptors in the oviduct tracts of Japanese quail (Coturnix coturnix japonica).

Neurotrophins (NGF, BDNF and NT-3) and their specific receptors (TrkA, TrkB and TrkC) were studied in the oviduct of egg laying quails. Neurotrophins (NTs) are mainly involved in the development and maintenance of neuronal populations in the central and peripheral nervous system, but also in reproductive system. In this survey, we first studied the morphological organization of the quail oviduct, distinguished in infundibulum, magnum, isthmus, uterus and vagina, and then we analyzed the expression and localization of NTs and Trks receptors in the whole tracts. By western blotting we detected that the investigated NTs and Trks receptors are expressed in all oviductal tracts. By immunohistochemistry we were able to define the distribution of NTs and Trks. Specifically, NGF, BDNF and NT3 were localized in lining and ductal epithelial cells, and NGF was also detected in secretory cells of tubular glands and in nervous fibers of vessel wall. TrkA and TrkB were present in the lining and ductal epithelium; TrkA and TrkC were present in nervous fibers of vessel wall in all oviductal tracts. Furthermore, we also observed NGF and BDNF co-localized with TrkA and TrkB in cells of the lining and ductal epithelium, suggesting an autocrine mechanism of action.

Expression of Cyclooxygenase-2 in naturally occurring bovine cutaneous fibropapillomas.

Bovine cutaneous fibropapillomas are benign skin tumours characterized by epithelial and dermal proliferation and induced by Bovine papillomaviruses (BPVs). Cyclooxygenase (COX) 1 and 2 are enzymes involved in pathological conditions, such as inflammation and epithelial carcinogenesis. Here we investigated biochemically and immunohistochemically COX-2 expression in bovine cutaneous fibropapillomas. Eight of twelve fibropapillomas (67%) showed COX-2 positive immunosignal mostly in the cytoplasm of the basal cell layer, while the normal skin did not stain. Biochemical analysis confirmed the expression of COX-2 in tumour samples. This study shows COX-2 expression in cutaneous fibropapillomas, suggesting a contribution in epithelial tumour development.

Characterization of the relations between morphology and physiological status of the pineal gland in connection with the somatic development level in turkeys reared in Romania / Caracterização das relações entre morfologia e estado fisiológico da glândula pineal em conexão com o nível de desenvolvimento somático em perus criados na România

This research started from the premises of the existence of some possible relationships between indole and pineal peptide hormones and the somatic development, with participation of hypothalamic-pituitary complex. Experimental factors, which were the subject of the present paper, influenced the dynamics of corporal mass and fodder consumption, leading to the occurrence of some important structural modifications at the level of pineal gland. The exposure of the individuals to continuous light (photic pinealectomy) produces increases in corporal mass, showing the involvement of the pineal gland in neuro-endocrine-metabolic reactions, which contributes to the maintenance of homeostatic balance, including somatic ones. Biological material was represented by a number of 50 individuals belonging to B.U.T. Big 6 hybrid, reared on soil, on a permanent litter, which could assure the expanding of knowledge area regarding the relation between rearing technology, modulation of some microclimate parameters and growing performances. Were also realised cytometric and hystometric muscular determinations.

A pesquisa começou a partir da premissa de que provavelmente existam relações entre indol e hormônios peptídicos pineal e o desenvolvimento somático, com a participação do complexo hipotálamo-hipófise. Fatores experimentais que foram objeto do presente trabalho influenciaram a dinâmica da massa corporal e o consumo de forragem, o que leva à ocorrência de algumas modificações estruturais importantes no nível da glândula pineal. A exposição dos indivíduos à luz contínua (Pinealectomia photic) leva ao aumento de massa corporal, mostrando o envolvimento da glândula pineal em reações neuroendócrino metabólicas, inclusive somáticas, que contribuem na manutenção do equilíbrio homeostático. O material biológico foi representado por um número de 50 indivíduos pertencentes a MAS Big híbrido 6, criados em solo, em uma ninhada permanente, o que poderia garantir a expansão da área de conhecimento a respeito da relação entre a tecnologia de criação, modulação de alguns parâmetros microclimáticos e performances de crescimento. Também foram realizadas por citometria e histometria determinações musculares.