Conjugation of dual-natural milk-derived proteins with fucoidan to prepare controllable glycosylation products via dielectric barrier discharge cold plasma.

This study reported that fibrillar bridges (whey protein isolate nanofibrils, WPNs) were used to associate the casein (CA) nanoparticles through the pH-driven method to obtain the self-assembled WPN-CA complexes. Then, a novel technology involving cold plasma (CP) was innovatively proposed to enhance the protective properties of complexes. The confirmation of structural transitions and interactions resulting from the adjustment of WPN-to-CA ratios (WtCs) led to the identification of the complexes named WPCA (WtC1.0:1). Next, the results showed a rapid conjugation between WPCA and fucoidan (FD) with a degree of grafting of 16.03 % after 10 min CP treatment. The coupling of WPCA with FD to form conjugates was confirmed by SDS-PAGE analysis, indicating covalent bonds' formation. FTIR spectroscopy revealed an augmentation in the intensity of the OH stretching vibration of the WPCA-FD conjugate, concomitant with a decrease in ß-turns and an elevation in ß-sheets content. Furthermore, the application of glycosylation treatment to WPCA-FD resulted in a noteworthy enhancement of both the thermal stability and antioxidant activity characteristics of WPCA. Our findings move a step forward, as CP-assisted Maillard reaction has shown potential as an efficient and energy-saving method to enhance the functional properties of milk-derived proteins in the food industry.

Mechanisms of Traditional Chinese medicine/natural medicine in HR-positive Breast Cancer: A comprehensive Literature Review.

ETHNOPHARMACOLOGICAL RELEVANCE: With the emergence of endocrine resistance, the survival and good prognosis of HR-positive breast cancer (HR + BC) patients are threatened. As a common complementary and alternative therapy in cancer treatment, traditional Chinese medicine (TCM) has been widely used, and its internal mechanisms have been increasingly explored. AIM OF THE REVIEW: In this review, the development status and achievements in understanding of the mechanisms related to the anti-invasion and anti-metastasis effects of TCM against HR + BC and the reversal of endocrine drug resistance by TCM in recent years have been summarized to provide ideas for antitumour research on the active components of TCM/natural medicine. METHODS: We searched the electronic databases PubMed, Web of Science, and China National Knowledge Infrastructure database (CNKI) (from inception to July 2023) with the key words "HR-positive breast cancer" or "HR-positive breast carcinoma", "HR + BC" and "traditional Chinese medicine", "TCM", or "natural plant", "herb", etc., with the aim of elucidating the intrinsic mechanisms of traditional Chinese medicine and natural medicine in the treatment of HR + BC. RESULTS: TCM/natural medicine monomers and formulas can regulate the expression of related genes and proteins through the PI3K/AKT, JAK2/STAT3, MAPK, Wnt and other signalling pathways, inhibit the proliferation and metastasis of HR + BC tumours, play a synergistic role in combination with endocrine drugs, and reverse endocrine drug resistance. CONCLUSION: The wide variety of TCM/natural medicine components makes the research and development of new methods of TCM for BC treatments more selective and innovative. Although progress has been made on research on TCM/natural medicine, there are still many problems in clinical and basic experimental designs, and more in-depth scientific explorations and research are still needed.

Compounds targeting ferroptosis in breast cancer: progress and their therapeutic potential.

In recent years, there has been a significant increase in the incidence of Breast cancer (BC), making it the most common cancer among women and a major threat to women's health. Consequently, there is an urgent need to discover new and effective strategies for treating BC. Ferroptosis, a novel form of cell death characterized by the accumulation of iron-dependent lipid reactive oxygen species, has emerged as a distinct regulatory pathway separate from necrosis, apoptosis, and autophagy. It is widely recognized as a crucial factor in the development and progression of cancer, offering a promising avenue for BC treatment. While significant progress has been made in understanding the mechanisms of ferroptosis in BC, drug development is still in its early stages. Numerous compounds, including phytochemicals derived from dietary sources and medicinal plants, as well as synthetic drugs (both clinically approved medications and laboratory reagents), have shown the ability to induce ferroptosis in BC cells, effectively inhibiting tumor growth. This comprehensive review aims to examine in detail the compounds that target ferroptosis in BC and elucidate their potential mechanisms of action. Additionally, the challenges associated with the clinical application of ferroptosis-inducing drugs are discussed, offering valuable insights for the development of novel treatment strategies for BC.

Potential risk of tamoxifen: gut microbiota and inflammation in mice with breast cancer.

Objective: Tamoxifen is an effective anti-tumor medicine, but evidence has been provided on tamoxifen-related inflammation as well as its impact on gut microbiota. In this study, we aimed to investigate tamoxifen-induced gut microbiota and inflammation alteration. Methods: We established a BC xenograft mouse model using the MCF-7 cell line. 16S rRNA gene sequencing was used to investigate gut microbiota. qRT-PCR, western blotting, and cytometric bead array were used to investigate inflammation-related biomarkers. Various bioinformatic approaches were used to analyze the data. Results: Significant differences in gut microbial composition, characteristic taxa, and microbiome phenotype prediction were observed between control, model, and tamoxifen-treated mice. Furthermore, protein expression of IL-6 and TLR5 was up-regulated in tamoxifen-treated mice, while the mRNA of Tlr5 and Il-6, as well as protein expression of IL-6 and TLR5 in the model group, were down-regulated in the colon. The concentration of IFN-γ, IL-6, and IL12P70 in serum was up-regulated in tamoxifen-treated mice. Moreover, correlation-based clustering analysis demonstrated that inflammation-negatively correlated taxa, including Lachnospiraceae-UCG-006 and Anaerotruncus, were enriched in the model group, while inflammation-positively correlated taxa, including Prevotellaceae_UCG_001 and Akkermansia, were enriched in the tamoxifen-treated group. Finally, colon histologic damage was observed in tamoxifen-treated mice. Conclusion: Tamoxifen treatment significantly altered gut microbiota and increased inflammation in the breast cancer xenograft mice model. This may be related to tamoxifen-induced intestinal epithelial barrier damage and TLR5 up-regulation.

Inattentional blindness to unexpected hazard in augmented reality head-up display assisted driving: The impact of the relative position between stimulus and augmented graph.

OBJECTIVE: An augmented reality head-up display (AR-HUD) is a promising technology in assisted driving. It provides additional information in the driving environment. However, considering the registration problem related to the limitations of interactive technology, we suspect that an AR-HUD may not be able to recognize unpredictable stimuli in a timely manner, inducing inattentional blindness to these non-augmented stimuli. Actually, non-augmented stimuli may accidentally have a brief superimposition to AR graphics. This condition may also influence the rate of inattentional blindness accordingly. Thus, this study examined the problem of inattentional blindness in AR-HUD systems that may result from the immaturity of AR technology. METHOD: We investigated the impact of AR graphic position (peripheral AOI v.s. central AOI) and the relative position of the AR graphic on unpredictable stimuli (on-HUD hazard v.s. off-HUD hazard) on the occurrence of inattentional blindness. Thirty Participants watched an AR-augmented driving video that included four augmented conditions. Participants were instructed to respond to four critical events (speeding, running of red lights, unexpected pedestrians or motorcycles). The rate of inattentional blindness and response time were recorded. We only analyzed data on unexpected pedestrian and motorcycle incidents. RESULTS: The relative position of the AR graphic on unpredictable stimuli and AR graphic positions significantly affected the rate of inattentional blindness and response time. Drivers had a higher rate of inattentional blindness to the unpredictable stimulus briefly superimposed on the AR graphic (i.e., on-HUD hazard) in the peripheral visual field (i.e., peripheral AOI). Also, drivers exhibited a higher rate of inattentional blindness to the unpredictable stimuli outside the AR graphic (i.e., off-HUD hazard) in the central visual field (i.e., central AOI). CONCLUSION: The study is expected to be beneficial for furthering the design of an AR-HUD-assisted system to reduce inattentional blindness in driving. Our results found that in the peripheral visual field, unpredictable stimuli accidentally superimposed on the AR graphic (i.e., on-HUD hazard) lead to a higher probability of ignoring the accidental events and seemed to require a longer response time for drivers. This study illustrated that inattentional blindness to non-augmented stimuli is also influenced by the AR graphic position when AR technology fails to augment them in a timely manner. An important recommendation emerging from this work is to consider the design of AR graphics according to the AR graphic positions and stimulus types to reduce the occurrence of inattentional blindness.

The expression of androgen receptor in triple-negative breast cancer and the effect of a traditional Chinese medicine formula on disease-free survival.

Background: Androgen receptor (AR) is becoming an important factor in the pathogenesis of breast cancer. Traditional Chinese medicine (TCM) is widely used in treating breast cancer patients. Triple-negative breast cancer (TNBC) is a subtype of breast cancer, which has worse prognosis than other subtypes. Herein, through this retrospective study, we summarize the therapeutic implications of AR and TCM in TNBC. Methods: The clinical and pathological data of TNBC patients who had undergone surgery at The First Affiliated Hospital of Zhejiang Chinese Medical University from 2017 to 2019 were collected and examined. The t-test, chi-square test, logistic regression model, and Kaplan-Meier survival estimates were used to analyze the data. Results: We identified 823 early breast cancer patients from January 2017 to December 2019, of whom 92 (11.2%) were pathologically confirmed to have TNBC. We excluded 5 patients according to the inclusion and exclusion criteria. In relation to the remaining 87 patients, 33 (37.9%) were AR positive. In the TNBC patients, positive AR expression was correlated with an older age (P=0.006), a higher weight (P=0.006), and lower Ki-67 expression (P=0.031). After a median follow-up time of 37 months (range, 24-60 months), 13 cases of relapse and metastasis (14.9%) were observed. We found that relapse and metastasis were correlated with being unmarried [P=0.004; hazard ratio (HR) =0.105; 95% confidence interval (95% CI): 0.023-0.487], nonporous (P=0.046; HR =0.209; 95% CI: 0.045-0.971), and negative AR expression (P=0.042; HR =1.223; 95% CI: 0.049-1.012). The AR-positive TNBC patients had better disease-free survival (DFS) than the AR-negative TNBC patients 2-5 years after surgery (P<0.05). TCM was an effective treatment for TNBC (P<0.001; HR =51.682; 95% CI: 6.660-401.025). In the AR-negative group, patients who received the TCM treatment tended to have a better DFS than those who did not receive the TCM treatment (P<0.001; HR =34.832; 95% CI: 4.448-272.756); however, no such difference was found in the AR-positive group. Conclusions: The TNBC patients with positive AR tended to have a low expression of Ki-67 and a better prognosis than AR negative TNBC patients. TCM is an effective treatment and has slight side effects.

Deoxynivalenol Biosynthesis in Fusarium pseudograminearum Significantly Repressed by a Megabirnavirus.

Deoxynivalenol (DON) is a mycotoxin widely detected in cereal products contaminated by Fusarium. Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1) is a double-stranded RNA virus infecting Fusarium pseudograminearum. In this study, it was revealed that the amount of DON in F. pseudograminearum was significantly suppressed by FpgMBV1 through a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay. A total of 2564 differentially expressed genes were identified by comparative transcriptomic analysis between the FpgMBV1-containing F. pseudograminearum strain FC136-2A and the virus-free strain FC136-2A-V-. Among them, 1585 genes were up-regulated and 979 genes were down-regulated. Particularly, the expression of 12 genes (FpTRI1, FpTRI3, FpTRI4, FpTRI5, FpTRI6, FpTRI8, FpTRI10, FpTRI11, FpTRI12, FpTRI14, FpTRI15, and FpTRI101) in the trichothecene biosynthetic (TRI) gene cluster was significantly down-regulated. Specific metabolic and transport processes and pathways including amino acid and lipid metabolism, ergosterol metabolic and biosynthetic processes, carbohydrate metabolism, and biosynthesis were regulated. These results suggest an unrevealing mechanism underlying the repression of DON and TRI gene expression by the mycovirus FpgMBV1, which would provide new methods in the detoxification of DON and reducing the yield loss in wheat.

Novel sonification designs: Compressed, iconic, and pitch-dynamic auditory icons boost driving behavior.

With the development of connected vehicles, in-vehicle auditory alerts enable drivers to effectively avoid hazards by quickly presenting critical information in advance. Auditory icons can be understood quickly, evoking a better user experience. However, as collision warnings, the design and application of auditory icons still need further exploration. Thus, this study aims to investigate the effects of internal semantic mapping and external acoustic characteristics (compression and dynamics design) on driver performance and subjective experience. Thirty-two participants (17 females) experienced 15 types of warnings - (3 dynamics: mapping 0 vs. 1 vs. 2) × (5 warning types: original iconic vs. original metaphorical vs. compressed iconic vs. compressed metaphorical auditory icon vs. earcon) - in a simulator. We found that compression design was effective for rapid risk avoidance, which was more effective in iconic and highly pitch-dynamic sounds. This study provides additional ideas and principles for the design of auditory icon warnings.

GeneChip expression profiling identified OLFML2A as a potential therapeutic target in TNBC cells.

Background: An elevated level of olfactomedin-like-2A (OLFML2A) is unfavorable for female breast cancer patients. Patients with a high mRNA level of OLFML2A receive a poor prognosis. Therefore, we speculate that inhibiting the expression of this gene may be beneficial to breast cancer patients. We previously found that silencing the OLFML2A gene by using mRNA interference significantly inhibited proliferation and migration in triple-negative breast cancer (TNBC) cells. Methods: Cell activity and proliferation were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Celigo analyses. Cell migration and invasion were determined by wound-healing and transwell invasion assays. The mechanism of the inhibition of a small hairpin RNA that targets OLFML2A (shOLFML2A) was determined by using a GeneChip array, real-time quantitative PCR (RT-qPCR), and western blot analysis. Results: Gene silencing by shOLFML2A induces apoptosis by promoting S phase arrest in TNBC cells. In addition, shOLFML2A decreased the progression of epithelial-mesenchymal transition (EMT). Additionally, microarray analysis showed that shOLFML2A significantly upregulated 428 genes and downregulated 712 genes. These significantly changed genes regulated DNA synthesis, chromosome alignment, microtubules and the cytoskeleton, cell movement, the cell cycle, cell necrosis, and apoptosis because they promoted G2/M DNA damage checkpoint regulation and p53 signaling, and because they inhibited integrin, hepatocyte growth factor (HGF), nerve growth Factor (NGF), and other tumor-promoting signaling pathways. Conclusions: shOLFML2A reduces cell proliferation, migration, and invasion and promotes cell apoptosis. Therefore, the results of the present study suggest that OLFML2A is a potential therapeutic target for TNBC.

The first report of Lasiodiplodia theobromae causing leaf spot on Illicium verum in China.

Star anise (Illicium verum) production is an important industry in parts of southern China (Wang et al. 2011). The production of star anise (cultivar Dahong) was seriously affected by a leaf spot disease during the summer of 2020 in Rong County, Guangxi province, China. Approximately 20% to 30% of the trees (n = 200, 15-16 years old) had obvious leaf spots on more than 80% of the leaves. Local growers had to cut down the seriously diseased trees. Symptomatic samples were collected and sent to us at the end of August 2021. A single brown-edged round gray spot appeared on each leaf. The spot was ~20 mm in diameter. The margin was cut into 5 mm pieces, then disinfested with 3% NaOCl for 30 s, 75% ethanol for 30 s and sterile deionized water for 1 min before dried and placed on potato dextrose agar (PDA) medium at 25°C in the dark. A total of 25 fungal isolates were obtained (isolation rate 82%). Genomic DNAs was extracted from the mycelia of these isolates and three diagnostic regions including ITS1/ITS4 (White et al. 1990), EF1-728F/EF1-986R (Carbone and Kohn 1999) and Bt2a/Bt2b (Glass and Donaldson 1995) were amplified. The colony morphology on PDA and the sequences of the five isolates BJ20-1, BJ20-4, BJ20-5, BJ20-7, BJ20-8 were identical. Fungal colonies had light gray mycelium and black pigment on PDA. The average colony growth rate was 4.25±0.31 cm per day and no spores were produced. Sequences of the representative isolate BJ20-1 were deposited in GenBank (Accession nos. OK483326, OL547596 and OL547597). BLASTn search indicated high identity 99.6%, 98.58% and 100% to ITS (AY640255), EF1-α (AY640258), and ß-tubulin (KU887532) of Lasiodiplodia theobromae, respectively. Combined phylogenetic analysis using MEGAX (Kumar et al. 2018) clustered BJ20-1 and L. theobromae CBS164.96 in one clade. To test pathogenicity, 2-years-old healthy I. verum trees (cultivar Dahong) maintained in a greenhouse were inoculated. Leaves were surface sterilized with 70% ethanol. One PDA plug (5mm in diameter) was placed on each wound acupunctured with a sterile needle pick. Ten PDA plugs with mycelial of BJ20-1 growing 7 days on it were inoculated on five leaves. Four sterile PDA plugs placed on two leaves served as controls. All the plugs were removed from the leaves the day after inoculation. The experiment was repeated three times. At 1 day post-inoculation (DPI), brown expanding lesions were observed on the inoculated leaves. At 7 DPI, a mature ellipse of necrosis formed with 18±4 mm in diameter with black pycnidia in the center. Conidia were observed in the pycnidia. The immature conidia were thick-walled, hyaline, aseptate and ellipsoid, measuring 20 - 25.2 × 11 - 13 µm (n = 25). The mature conidia were dark brown with one central septum, measuring 24.3 - 27 × 13 - 14 µm (n = 25). At 10 DPI, the control leaves remained asymptomatic. Re-isolation was successful from the spot on the inoculated leaves. The colony morphology and molecular identification of the re-isolations were all the same as that of BJ20-1. In conclusion, the morphological and molecular evidence consistently indicated these isolates were L. theobromae. Koch's postulates were fulfilled that L. theobromae was pathogenic on star anise. Although L. theobromae has been reported to cause leaf spot disease on Camellia sinensis (Bao et al, 2021), Kadsura longipedunculata (Fan et al, 2020) and Broussonetia papyrifera (Luo et al, 2020), etc., this is the first report of L. theobromae causing leaf spot on I. verum in China. Due to the leaf spot disease resulting in serious yield reduction on star anise, accurate pathogen identification in this study would significantly improve the control of the leaf spot disease on star anise.

Amorphophalli Rhizoma inhibits breast cancer growth, proliferation, migration, and invasion via the PI3K/AKT pathway.

ETHNOPHARMACOLOGICAL RELEVANCE: Amorphophalli Rhizoma (APR) is widely used as an adjuvant treatment for advanced and metastatic triple-negative breast cancer (TNBC), but its effects, potential active ingredients, and mechanism of action on estrogen receptor-positive (ER+) and human epidermal growth factor receptor-positive (HER2+) breast cancer cells were not reported. AIM OF THE STUDY: The present study investigated the effects and mechanism of APR on ER+ and HER2+ breast cancer cells. MATERIALS AND METHODS: Rotary evaporation was used to prepare different extracts of APR. Cell activity was assessed using the cell counting kit-8 (CCK-8) method. Wound healing assays were used to assess cell migration, and a cell invasion assay was performed using a Transwell chamber with Matrigel matrix. A xenograft model was used to analyze the inhibitory effects of APR on tumor growth. Bioinformatics analyses were used to explore the potential mechanism of APR in breast cancer. RT-qPCR and Western blotting were performed to reveal the molecular mechanism. RESULTS: The ethyl acetate extract of APR showed the strongest tumor inhibitory effect on ER+ and HER2+ breast cancer cells compared to petroleum ether or N-butanol extracts. APR inhibited ER+ and HER2+ breast cancer cell growth, proliferation, migration, and invasion via the phosphatidylinositol-3 kinase (PI3K)/AKT pathway. CONCLUSIONS: APR had a significant inhibitory effect on ER+ and HER2+ breast cancer cells via the PI3K/AKT signaling pathway. Therefore, APR may be useful for preventing ER+ and HER2+ breast tumor growth, proliferation, migration, and invasion.

Preparation of Carbon-Covered Phosphorus-Modified Alumina with Large Pore Size and Adsorption of Rhodamine B.

In this study, phosphorus-modified alumina with large pore size was synthesized through a coprecipitation method. The carbon-covered, phosphorus-modified alumina with large pores was prepared by impregnating with glucose and carbonizing to further improve the adsorption of organic dyes. The morphology and structure of these composites were characterized by various analysis methods, and Rhodamine B (RhB) adsorption was also examined in aqueous media. The results showed that the specific surface area and pore size of the phosphorus-modified alumina sample AP7 (prepared with a P/Al molar ratio of 0.07) reached 496.2 m2·g-1 and 21.9 nm, while the specific surface area and pore size of the carbon-covered phosphorus-modified alumina sample CAP7-27 (prepared by using AP7 as a carrier for glucose at a glucose/Al molar ratio of 0.27) reached 435.3 m2·g-1 and 21.2 nm. The adsorption experiment of RhB revealed that CAP7-27 had not only an equilibrium adsorption capacity of 198 mg·g-1, but also an adsorption rate of 162.5 mg·g-1 in 5 min. These superior adsorption effects can be attributed to the similar pore structures of CAP7-27 with those of alumina and the specific properties with those of carbon materials. Finally, the kinetic properties of these composites were also studied, which were found to be consistent with a pseudo-second-order kinetic model and Langmuir model for isothermal adsorption analysis. This study indicates that the prepared nanomaterials are expected to be promising candidates for efficient adsorption of toxic dyes.

Synthesis and Characterization of a New Aluminosilicate Molecular Sieve from Aluminosilica Perhydrate Hydrogel.

A novel structure aluminosilicate molecular sieve, named BUCT-3, was prepared by dynamic hydrothermal synthesis, and the critical factor to obtain the new structure is using an active silicon and aluminum source, aluminosilica perhydrate hydrogel. Meanwhile, only high content of O-O bonds can ensure the pure phase of BUCT-3. Through the characterization of x-ray powder diffraction (XRD), Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), and so on, some structure and morphology information of BUCT-3 molecular sieves as well as the special silicon and aluminum source was obtained. It's worth noticing that the O-O bonds of reactants can be reserved in the products, and thus, help us to get a new structure with cell parameters a = 8.9645 Å, b = 15.2727 Å, c = 11.3907 Å, α = 90°, ß = 93.858°, γ = 90°. The crystal system is monoclinic. Though the thermostability of BUCT-3 is not satisfactory, its potential application derived from O-O bonds cannot be neglected.

Removal of Phosphorus from Wastewater by Different Morphological Alumina.

In this work, an organic-free method was used to synthesize different morphological boehmite by controlling the crystallization temperature, and alumina adsorbents were obtained by baking the boehmites at 500 °C. The alumina adsorbents were characterized by X-ray diffraction (XRD), High resolution transmission electron microscope (HRTEM), Fourier transform infrared (FT-IR), N2 adsorption/desorption analysis, and their phosphorus adsorption properties were comparatively investigated by a series of experiments. The results showed that the self-prepared alumina adsorbents were lamellar and fibrous material, while the industrial adsorbent was a granular material. The lamellar alumina adsorbents had the largest specific surface area and showed better phosphorus adsorption capacity. The maximum adsorption capacity could reach up to 588.2 mg·g-1; and only 0.8 g·L-1 of lamellar alumina adsorbent is needed to treat 100 mg·L-1 phosphorus solution under the Chinese level 1 discharge standard (0.5 mg·L-1). Further investigation suggests that the lamellar alumina adsorbent kept high adsorption capacity in various solution environments.

Synthesis and characterization of germanosilicate molecular sieves: GeO2/SiO2 ratio, H2O/TO2 ratio and temperature.

Many synthesis parameters can influence zeolite crystallization, which include the molar ratio of reagents, water content, temperature, the selection of extraframework cations (organic or inorganic template) and so on. In this paper, two new materials, BUCT-1 with cuboid morphology and BUCT-2 with plate-like morphology were obtained based on the synthesis conditions of ITQ-17 by adjusting the GeO2/SiO2 ratio, H2O/TO2 ratio and temperature. The influence of the three factors on crystal size, crystallinity and phase selection and transformation was carefully discussed. Therein, phase selection and transformation is determined by their synergistic effects; while the influence on crystal size and crystallinity is different for different materials, which is caused by the differences in their structure, or in other words, the building unit, and chemical composition. In addition, the structure of BUCT-2 has already been solved as the stacking of sti layers, and it was identified as pure germanate molecular sieves. Meanwhile, through the characterization of XRD, ICP-AES, IR and STA, some topological information on BUCT-1, such as unit cell parameters, pore size and connectivity, was predicted and the work to present its final structure is still going on.