Exploring the Biological Pathways of Siderophores and Their Multidisciplinary Applications: A Comprehensive Review.

Siderophores are a class of small molecules renowned for their high iron binding capacity, essential for all life forms requiring iron. This article provides a detailed review of the diverse classifications, and biosynthetic pathways of siderophores, with a particular emphasis on siderophores synthesized via nonribosomal peptide synthetase (NRPS) and non-NRPS pathways. We further explore the secretion mechanisms of siderophores in microbes and plants, and their role in regulating bioavailable iron levels. Beyond biological functions, the applications of siderophores in medicine, agriculture, and environmental sciences are extensively discussed. These applications include biological pest control, disease treatment, ecological pollution remediation, and heavy metal ion removal. Through a comprehensive analysis of the chemical properties and biological activities of siderophores, this paper demonstrates their wide prospects in scientific research and practical applications, while also highlighting current research gaps and potential future directions.

Anoikis-related gene signatures in colorectal cancer: implications for cell differentiation, immune infiltration, and prognostic prediction.

Colorectal cancer (CRC) is a malignant tumor originating from epithelial cells of the colon or rectum, and its invasion and metastasis could be regulated by anoikis. However, the key genes and pathways regulating anoikis in CRC are still unclear and require further research. The single cell transcriptome dataset GSE221575 of GEO database was downloaded and applied to cell subpopulation type identification, intercellular communication, pseudo time cell trajectory analysis, and receptor ligand expression analysis of CRC. Meanwhile, the RNA transcriptome dataset of TCGA, the GSE39582, GSE17536, and GSE17537 datasets of GEO were downloaded and merged into one bulk transcriptome dataset. The differentially expressed genes (DEGs) related to anoikis were extracted from these data sets, and key marker genes were obtained after feature selection. A clinical prognosis prediction model was constructed based on the marker genes and the predictive effect was analyzed. Subsequently, gene pathway analysis, immune infiltration analysis, immunosuppressive point analysis, drug sensitivity analysis, and immunotherapy efficacy based on the key marker genes were conducted for the model. In this study, we used single cell datasets to determine the anoikis activity of cells and analyzed the DEGs of cells based on the score to identify the genes involved in anoikis and extracted DEGs related to the disease from the transcriptome dataset. After dimensionality reduction selection, 7 marker genes were obtained, including TIMP1, VEGFA, MYC, MSLN, EPHA2, ABHD2, and CD24. The prognostic risk model scoring system built by these 7 genes, along with patient clinical data (age, tumor stage, grade), were incorporated to create a nomogram, which predicted the 1-, 3-, and 5-years survival of CRC with accuracy of 0.818, 0.821, and 0.824. By using the scoring system, the CRC samples were divided into high/low anoikis-related prognosis risk groups, there are significant differences in immune infiltration, distribution of immune checkpoints, sensitivity to chemotherapy drugs, and efficacy of immunotherapy between these two risk groups. Anoikis genes participate in the differentiation of colorectal cancer tumor cells, promote tumor development, and could predict the prognosis of colorectal cancer.

The clinical significance of complete process management for the quality control of horizontal rotational resection of a breast mass.

Objective: To explore the clinical outcomes of the complete process management of horizontal rotational resection of a breast mass. Methods: A retrospective study was conducted involving 638 patients who underwent horizontal rotational resection of breast tissue in the Department of Thyroid and Breast Surgery of the People's Hospital of China Medical University from August 2018 to August 2020 using the ultrasound Breast Imaging-Reporting and Data System (BI-RADS) classification of 4A and below. These patients were divided into the experimental group and the control group based on whether the surgery had been performed following the order of the complete process management. The time cutoff point for the two groups was June 2019. The propensity score matching method was used to implement 1:1 ratio matching according to age, mass size, location, ultrasound BI-RADS classification, and breast size (measured by basal diameter), and the patients in the two groups were compared for the duration of surgery (the time needed to performed the three-step 3D positioning), postoperative skin hematoma and ecchymosis, postoperative pathological malignancy rate, residual rate of the mass, and satisfaction rate. Results: After 278 pairs were matched, no statistically significant differences were found between the two groups in terms of demographics (P > 0.05). The duration of surgery in the experimental group was significantly shorter compared with the control group (7.90 ± 2.18 min vs. 10.20 ± 5.99 min, respectively; P < 0.05); the satisfaction score in the experimental group (8.33 ± 1.36) was higher compared with the control group (6.48 ± 1.22) (P < 0.05); the malignant and residual rates of mass in the experimental group were lower than those in the control group, i.e., 6 vs. 21 cases (P < 0.05), and 4 vs. 16 cases, respectively (P < 0.05); the incidence of skin hematoma and ecchymosis was lower in the experimental group, i.e., 3 vs. 21 cases (P < 0.05). Conclusion: Complete process management for horizontal rotational resection of a breast mass can shorten the duration of surgery, reduce the residual mass, postoperative bleeding, and postoperative malignancy rates, and improve the breast preservation rate and patient satisfaction. Accordingly, its popularization represents research value.

Horizontal Rotary Cutting Technique for Skin Injury Avoidance in Superficial Benign Breast Masses.

OBJECTIVE: This study aims to investigate the feasibility of the horizontal rotary-cut technique in the removal of superficial benign breast tumors with a ≤1.0 cm distance between the upper margin of the tumor and the skin. PATIENTS AND METHODS: A total of 69 patients with superficial benign breast tumors received horizontal rotary-cut surgery between July 2018 and June 2019 (horizontal group). The rotary cutter groove was in the true lateral position of the tumor and the ultrasonic probe was vertical to the rotary cutter groove. The patients were compared with 33 patients who underwent the traditional vertical rotary-cut surgery between July 2017 and June 2018 (traditional group) regarding the aspects of operation time, intraoperative bleeding volume, postoperative skin ecchymosis, skin damage, and tumor residue. The rotary cutter groove was directly below the tumor and the ultrasonic probe was parallel to the rotary cutter groove in the traditional vertical rotary-cut surgery. RESULTS: The operation time in the horizontal group was significantly shorter than in the traditional group (7.7 ± 1.1 minutes vs 9.5 ± 1.3 minutes, with t = -7.458 and p = 0.000) and there was significantly less skin damage in the horizontal group than in the traditional group (0 cases vs 3 cases, with p = 0.032). The differences in intraoperative bleeding and postoperative skin ecchymosis between the two groups were not statistically significant (6.0 ± 1.3 mL vs 6.5 ± 1.5 mL, with t = -1.853 and p = 0.067; 4 cases vs 2 cases, with χ 2 = 0.003 and p = 0.958). Ninety-seven patients attended follow-ups for 6-30 (16.5 ± 4.5) months. No residues or recurrences were observed under ultrasound reviews in either group. CONCLUSION: In superficial benign breast tumor removal, the horizontal rotary-cut breast technique can help avoid skin injury, shorten the operation time, and reduce tumor residue more effectively compared with the traditional vertical rotary-cut technique. It has certain popularization and application values.

Treatment strategy for chylous leakage after dissection of central lymph nodes in thyroid cancer.

OBJECTIVE: To explore the treatment strategies of chylous leakage after dissection of central lymph nodes in thyroid cancer. METHODS: Patients with chylous leakage after dissection of central lymph nodes in thyroid cancer were recruited in this study. All participants adopted a conservative treatment method of external fixation of neck brace + cotton ball packing and compression bandage + adequate negative pressure drainage and assisted low-fat diet. RESULTS: A total of two patients were included in this study. The results showed that the drainage rate of these two patients was reduced to less than 10 ml after 1-2 days of drainage. The maintenance treatment was continued for 10 days. The drainage volume of the patient did not increase during the three days after returning to normal diet. There was no recurrence of posterior lymphatic leakage. CONCLUSION: External fixation of neck brace + cotton ball packing and compression bandage + adequate negative pressure drainage is important for the treatment of mild to moderate chylotrhea after thyroid cancer surgery which was worthy of clinical application. KEY WORDS: Chylous leakage; Dissection of lymph nodes; Thyroid cancer; External fixation of neck brace; Postoperative complication.

Visualization of Fluoride Ions In Vivo Using a Gadolinium(III)-Coumarin Complex-Based Fluorescence/MRI Dual-Modal Probe.

A new Gadolinium(III)-coumarin complex, DO3A-Gd-CA, was designed and prepared as a dual-modal probe for simultaneous fluorescence and relaxivity responses to fluoride ions (F-) in aqueous media and mice. DO3A-Gd-CA was designed by using Gd(III) center as an MRI signal output unit and fluoride binding site, and the 4-(diethylamino)-coumarin-3-carboxylic acid (CA) as a fluorescence reporter. Upon the addition of fluoride ions to the solution of DO3A-Gd-CA, the liberation of the coordinated CA ligand led to a 5.7-fold fluorescence enhancement and a 75% increase in the longitudinal relaxivity (r1). The fluorescent detection limit for fluoride ions was determined to be 8 µM based on a 3σ/slope. The desirable features of the proposed DO3A-Gd-CA, such as high sensitivity and specificity, reliability at physiological pH and low cytotoxicity enable its application in visualization of fluoride ion in mice. The successful in vivo imaging indicates that DO3A-Gd-CA could be potentially used in biomedical diagnosis fields.

A gadolinium(iii) complex based dual-modal probe for MRI and fluorescence sensing of fluoride ions in aqueous medium and in vivo.

A novel Gd(iii) complex, Gd(TTA)3-DPPZ, was designed and assembled as a dual-modal probe for the simultaneous fluorescence and magnetic resonance imaging (MRI) detection of fluoride ions in aqueous media and in vivo. In this system, the Gd(iii) center is not only serving as a MRI signal output unit, but also as a binding site for fluoride ions. When appropriate equivalents of fluoride ions were added into the solution of Gd(TTA)3-DPPZ, the replacement of the coordination water led to a decrease of the longitudinal relaxivity (r1) as well as distinct spectroscopic changes, by which MRI/fluorescence dual-modal fluoride ion sensing was achieved. In the presence of fluoride ions, a 2-fold fluorescence emission enhancement of Gd(TTA)3-DPPZ, and a notable decrease of the UV-vis absorption spectrum were observed. The fluorescence detection limit for fluoride ions was established at 70 nM. Gd(TTA)3-DPPZ also exhibits about 75% decrease of the longitudinal relaxivity (r1) upon addition of fluoride ions in aqueous medium. The appropriate blood circulation time of Gd(TTA)3-DPPZ allows its potential application in MRI in vivo. The results demonstrated that Gd(TTA)3-DPPZ could serve as a potential MRI/fluorescence bimodal imaging agent for the specific and high-sensitive sensing of fluoride ions in vivo.

Fluoride-specific fluorescence/MRI bimodal probe based on a gadolinium(iii)-flavone complex: synthesis, mechanism and bioimaging application in vivo.

Molecular imaging is a powerful tool to visualize cellular processes and activity levels of biomarkers at the cellular and molecular level, in which a molecular probe is a prerequisite for the molecular imaging technique. The present work reports a bimodal probe for the fluorescence and magnetic resonance detection of fluoride ions (F-) in aqueous media and in vivo. The bimodal probe, EDTA-Gd-HF, was prepared by self-assembly of the EDTA-Gd complex with 3-hydroxyflavone (HF). In the system, HF plays the role of spectroscopic reporter and Gd(iii) serves as a MRI signal output unit as well as the fluoride ion binding site. Upon addition of fluoride ions into an aqueous solution of EDTA-Gd-HF, the replacement of one coordinated water molecule and then liberation of HF led to remarkable spectroscopic recovery and distinct changes to the longitudinal relaxivity (r1), by which fluorescence/MRI bimodal fluoride ion sensing was achieved. In vivo magnetic resonance imaging (MRI) in mice indicates that EDTA-Gd-HF can be potentially used in biomedical diagnosis and therapy fields.