[Molecular-biological nature of morphological abnormalities induced by chronic irradiation in coniferous plants from the Chernobyl nuclear power plant exclusion zone: emphasis on a possible role of the cytoskeleton]. / Molekuliarno-biologicheskaia priroda morfologicheskikh anomalii sredi golosemennykh rastenii, indutsirovannykh khronicheskim oblucheniem v zone otchuzhdeniia Chernobyl'skoi AES: aktsent na vozmozhnuiu rol' tsitoskeleta.

Results of the analysis of morphological abnormalities in coniferous plants from the Chernobyl exclusion zone are described. It is shown that such features as total and individual protein content, genome organization and peculiarities of its expression, and karyotype are different for the control and morphologically abnormal needles. A possible role of the cytoskeleton structures in the abnormal morphogenesis in plants is discussed.

[Molecular and cellular aspects of development of arbuscular mycorrhizal symbiosis and its role in plant life]. / Molekuliarnye i kletochnye aspekti razvitiia arbuskuliarnykh mikoriznykh simbiozov i ikh znachenie v zhiznedeiatel'nosti rastenii.

Development of arbuscular mycorrhizal (AM) symbiosis with plant root system in term of molecular and cellular events have been analysed. A role of AM symbiosis in plant life has been discussed. Molecular methods for analysis of arbuscular mycorrhizal fungi have been described.

[Radiation-induced changes in the genome of pine seeds, forming under conditions of chronic irradiation in the Chernobyl exclusion zone]. / Radiatsionno-indutsirovannye izmeneniia v genome semian sosny, sformirovabshikhsiia v usloviiakh khronicheskogo oblucheniia v zone otchuzhdeniia ChAES.

Comparative study of long-term existing genome damages in the chronically irradiated pine seeds from different parts of Chernobyl zone have been performed by a pulsed field gel electrophoresis and hydroxyapatite chromatography. Some differences were established for DNA degradation kinetic from protoplasts with various accumulated doses of irradiation. Results obtained could testify the existence of long-living changes in the experimental plants genome.

[The characteristics of the protein composition of the anomalous needles of spruce (Picea abies) and pine (Pinus silvestris) from the 10-kilometer zone of the Chernobyl Atomic Electric Power Station]. / Osobennosti belkovogo sostava anomal'noi khvoi eli (Picea abies) i sosny (Pinus silvestris) iz 10-kilometrovoi zony Chernobyl'skoi AES.

Comparative analysis for different antibodies' binding sites at the proteins samples, which were isolated from control and morphologically abnormal coniferous plants tissues have been investigated. Monoclonal antibodies against cytoskeletal proteins (actin, alpha- and beta tubulin), hsp 70 and against phosphotyrosine were used. It was detected that investigated biochemical features reflect morphological similarities between different plant species. Some differences between cytoskeleton proteins content in control and abnormal tissues have been also indicated. It is assumed that abnormal morphogenesis may be determined by low-level chronic irradiation during plants growth and development.

[Plant metallothioneins]. / Metallotioneiny rastenii.

The review is devoted to the problem of metal-binding proteins (metallothioneins) in plant objects. The chronology of studies, dealt with search of metallothionein-like proteins in plant tissues is described. Data about the structure and function of metallothioneins, the features of metallothioneins-like gene expression in plants is shown. The role of metallothioneins as stress proteins, which are involved in formation of plant reaction on the influence of different stress factors, is discussed.

[Antimitotic compounds as modifiers of the radiation lesion of cultured cells]. / Antimitoticheskie soedineniia kak modifikatory luchevogo porazheniia kul'tiviruemykh kletok.

The features of antimitotic substances as radioprotectors were studied. In vitro experiments have demonstrated that taxol revealed radioprotective features concerning the process of polymerization of irradiated microtubules. These results were the basis for the use of taxol and some other substances with high affinity for cytoskeleton proteins as potential radiomodificators in vivo. Experiments with cultivated fibroblasts revealed that colchicine significantly enhances radioactive injuries of cells while taxol and phalloidin manifest their radioprotective features.

[Assembly of microtubules in vitro as affected by high temperatures]. / Osobennosti sborki mikrotrubochek in vitro pri deistvii povyshennykh temperatur.

The process of polymerization of microtubules isolated from bovine brain by two polymerization-depolymerization cycles has been investigated at temperatures 41 and 45 degrees C. The damages of the polymerization process using the registration of the optical solution density are shown. The electron microscopic analysis has shown the damages in the structure of microtubules formed at high temperatures.