Smart control of soil water and salt content for improving irrigation management of tomato crop field: Kairouan area.

A good assessment of soil water and salt content is required for sustainable irrigation with brackish/saline water. The use of the Internet of Things (IoT) has been initiated for the tomato crop (Savera variety) as part of the PRIMA MEDITOMATO project. An experiment was carried out between February and June 2022 at a farmer's site. For continuous soil water and salt content assessment, TEROS (11/12) probes were implemented at depths of 0, 10, 20, 30, and 60 cm. The data logging process was performed by a ZL6 device and delivered by the ZENTRA Cloud web application (METER GROUPE Company). For the accuracy of the introduced sensors, calibration tests were first processed. Results of the calibration of the probes in the laboratory and in situ showed linear relationships between the humidity values measured by ZL6 (θZL6) and those determined by the gravimetric method, with high correlation coefficients (R2) of 0.86 and 0.96, respectively. There were also strong linear relationships between the ECbulk(ZL6) and the ECe measured on saturated paste extract with high correlation coefficients (R2) of 0.96 and 0.95. Corrected data, according to the determined linear regression equations, present the real-time assessment of soil water and salt content over the entire growth stage of tomatoes. The results of this monitoring showed that soil water content remained close to its status at field capacity (32%) at the beginning of the assessment and increased with the intensification of irrigation, reaching 46 and 54% at 20 and 30 cm, respectively, around mid-April. The salinity level was greater with depth. Indeed, it was low in topsoil with the increase in irrigation frequency and higher at 30 and 60 cm toward the end of the tomato cycle. According to this study, real-time data given by ZENTRA Cloud allows us to adjust irrigation management on time.

The nutritional composition of six plant species after irrigation with treated wastewater and possible hazards by heavy metal accumulation.

A field experiment was conducted investigating the possibility of using treated wastewater (TWW) on sites affected by water scarcity in summer, waterlogging during the wet season, and salinity. A corresponding pot experiment was conducted comparable to the field experiment in Kalaât Landelous. The same plant species (Atriplex nummularia Lindl., Eucalyptus gomphocephala DC., Acacia cyanophylla Lindl., Casuarina glauca Sieber ex Spreng., Cupressus sempervirens L., and Pinus halepensis Mill.) were grown with the same treatments. While, in the field the plants, elemental composition cannot be linked to inputs by TWW, this was studied under controlled conditions. Additionally, a control was established lower in salinity receiving tap water. The effect of TWW irrigation on macro- and microelement uptake by the six plant species was studied. The treatments were high soil salinity under drained saline (DS) conditions, high salinity under waterlogged saline (WS), and a drained non-saline control (DNS: EC = 3.0 dS/m, pH = 8.4). TWW application under DS treatment increased Na, Cl, Ca, Mg, N, P, and K in most plant tissues compared to the control. TWW application in WS treatment resulted in an increase in heavy metals. Cu and Zn showed the highest bioaccumulation factor (BAF). The BAF in different plant tissues followed the order: Cu > Zn > Mn > Cd > Ni > Co > Pb. The plants accumulated significant amounts of metals in their roots.

Vicia-Micronucleus Test Application for Saline Irrigation Water Risk Assessment.

In view of climate change, increasing soil salinity is expected worldwide. It is therefore important to improve prediction ability of plant salinity effects. For this purpose, brackish/saline irrigation water from two areas in central and coastal Tunisia was sampled. The water samples were classified as C3 (EC: 2.01-2.24 dS m-1) and C4 (EC: 3.46-7.00 dS m-1), indicating that the water was questionable and not suitable for irrigation, respectively. The water samples were tested for their genotoxic potential and growth effects on Vicia faba seedlings. Results showed a decrease in mitotic index (MI) and, consequently, growth parameters concomitant to the appearance of micronucleus (MCN) and chromosome aberrations when the water salinity increased. Salt ion concentration had striking influence on genome stability and growth parameters. Pearson correlation underlined the negative connection between most ions in the water inappropriate for irrigation (C4) and MI as well as growth parameters. MI was strongly influenced by Mg2+, Na+, Cl-, and to a less degree Ca2+, K+, and SO42-. Growth parameters were moderately to weakly affected by K+ and Ca2+, respectively. Re-garding MCN, a very strong positive correlation was found for MCN and K+. Despite its short-term application, the Vicia-MCN Test showed a real ability to predict toxicity induced by salt ions confirming that is has a relevant role in hazard identification and risk assessment of salinity effects.

Genus Sophora: a comprehensive review on secondary chemical metabolites and their biological aspects from past achievements to future perspectives.

Sophora is deemed as one of the most remarkable genera of Fabaceae, and the third largest family of flowering plants. The genus Sophora comprises approximately 52 species, 19 varieties, and 7 forms that are widely distributed in Asia and mildly in Africa. Sophora species are recognized to be substantial sources of broad spectrum biopertinent secondary metabolites namely flavonoids, isoflavonoids, chalcones, chromones, pterocarpans, coumarins, benzofuran derivatives, sterols, saponins (mainly triterpene glycosides), oligostilbenes, and mainly alkaloids. Meanwhile, extracts and isolated compounds from Sophora have been identified to possess several health-promising effects including anti-inflammatory, anti-arthritic, antiplatelets, antipyretic, anticancer, antiviral, antimicrobial, antioxidant, anti-osteoporosis, anti-ulcerative colitis, antidiabetic, anti-obesity, antidiarrheal, and insecticidal activities. Herein, the present review aims to provide comprehensive details about the phytochemicals and biological effects of Sophora species. The review spotlighted on the promising phytonutrients extracted from Sophora and their plethora of bioactivities. The review also clarifies the remaining gaps and thus qualifies and supplies a platform for further investigations of these compounds.

Salinity decreases cadmium accumulation in Vicia faba.

The study investigates the effect of cadmium (Cd), salinity (NaCl), and combined stress on rhizosphere pH, growth parameters, membrane leakage, and genotoxicity in Vicia faba. Germinated seeds were exposed for 48 h to 0.01 mM Cd(NO3)2 (Cd), 50 mM NaCl (S50), 150 mM NaCl (S150), and Cd-NaCl (CdS50 and CdS150). An accumulation of Cd and Na was found essentially in Vicia roots under each single stress factor associated with variations in rhizosphere pH. Additional NaCl in metallic solution significantly dropped the rhizosphere pH and decreased Cd concentrations in roots by 2.3 and 3.8 times for CdS50 and CdS150, respectively. Growth parameters (root length and fresh and dry matters), mitotic activity, and micronucleus formation were not influenced by Cd and low concentration of NaCl when applied separately or together, while 150 mM of NaCl, alone or combined with Cd, affected negatively all the studied parameters, as well as chromosome and nucleus stability. V. faba seems to reduce the transport of Cd in saline conditions and therefore salinity (50 mM) may act as a protection against Cd accumulation.

Saline soil reclamation by agroforestry species under Kalaât Landelous conditions and irrigation with treated wastewater in Tunisia.

Irrigation with treated waste water (TWW) in combination with plantation of agroforest species was tested in the Kalaât Landelous region for the reclamation of salt affected soils. Five species (Atriplex nummularia, Eucalyptus gomphocephala, Acacia cyanophylla, Casuarina glauca, Pinus halepensis) were cultivated in saline soils that are affected by shallow, saline groundwater and were irrigated with TWW during the summer season. The results after 4 years of experimentation show a distinct decrease in soil pH and salinity accompanied by a decrease in Cl and Na concentrations. Irrigation decreased the heavy metal concentrations in the topsoil but an increase in deeper layers indicate to leaching due to TWW irrigation. The investigated plant species were differently affected in growth performance by salinity and TWW irrigation. Atriplex nummularia appeared to be the most resistant species and Pinus halepensis the most sensitive one to hydro-pedological conditions of the Kalaât Landelous plot. In conclusion, salt-tolerant plant species seem to be good candidates for the reclamation of salt-affected, waterlogged sites in combination with TWW irrigation, as the adaptations of such species seem to operate under different abiotic stress conditions.

Chromosomal and Nuclear Alterations in the Root Tip Cells of Vicia faba Induced by Sodium Chloride.

Despite the well-known toxic effect of chloride sodium (NaCl), only few studies analyze its genotoxic effect on crops. Micronucleus assay is an efficient tool for the measure of chromosomal DNA damages. Based on this test, the effects of salt on micronucleus induction, and on chromosome and nucleus changes, were investigated in Vicia faba root tips treated with NaCl (electrical conductivity = 25 dS/m) during 24 hours. V. faba meristematic cells showed a significant increase in the number of micronuclei compared with the control. Micronucleus size varied from small to large and appeared at different stages of mitosis. Moreover, different chromosomal aberrations like breaks, bridges, rings, vagrant, and lagging chromosomes, were observed in the metaphase, the anaphase, and the telophase. In addition, nuclear alterations in the interphase were characterized by the presence of binucleated cells, strap nuclei with lesions, hyperchromasia, buds, and nuclei with irregular shapes and lesions.

Synergistic health effects between chemical pollutants and electromagnetic fields.

Humans and ecosystems are exposed to highly variable and unknown cocktail of chemicals and radiations. Although individual chemicals are typically present at low concentrations, they can interact with each other resulting in additive or potentially synergistic mixture effects. This was also observed with products obtained by radiation actions such as sunlight or electromagnetic fields that can change the effects of chemicals, such as pesticides, and metal trace elements on health. Concomitant presence of various pesticides and their transformation products adds further complexity to chemical risk assessment since chronic inflammation is a key step for cancer promotion. Degradation of a parent molecule can produce several by-products which can trigger various toxic effects with different impacts on health and environment. For instance, the cocktail of sunlight irradiated sulcotrione pesticide has a greater cytotoxicity and genotoxicity than parent molecule, sulcotrione, and questions about the impact of photochemical process on environment. Adjuvants were shown to modify the biological features of pesticides. Addition of other elements, metals or biological products, can differently enhance cell toxicity of pesticides or electromagnetic radiations suggesting a synergy in living organisms. Electromagnetic fields spreading, pesticide by-products and mixtures monitoring become greater for environmental contamination evaluations.

Expression of stress-related genes in tomato plants exposed to arsenic and chromium in nutrient solution.

The molecular responses of hydroponically cultivated tomato plants to As(V) or Cr(VI) were assessed by transcript accumulation analysis of genes coding for products potentially involved in heavy metal tolerance. A quantitative real-time PCR experiment was performed with Hsp90-1, MT2- and GR1-like protein genes using RNA isolated from tomato roots or shoots treated for 24h with As(V) or Cr(VI) at concentrations ranging from 80 to 640 microM. Both transient metallic treatments induced Hsp90-1 transcript accumulation in tomato plants. MT2- and GR1-like transcripts accumulated in tomato roots treated with As(V) but were only slightly affected by Cr(VI) treatment. Tomatoes showed phenotypic symptoms to heavy metal toxicity when plants were exposed to Cr(VI) but not As(V). Plant lethality was observed at 1280 microM Cr(VI), indicating that tomatoes were more tolerant to As than Cr stress under the experimental conditions used here.