[Effect of kontrikal on the dynamics of proteolytic system indices in postischemic toxemia]. / Vliianie kontrikala na dinamiku pokazatelei sistemy proteoliza pri postishemicheskoi toksemii.

It was shown in Wistar male rats that the development of tourniquet shock was followed by an increase of proteolytic activity in the blood by 3 times, activity of aspartate aminotransferase (AST) by 3 times, that of alanine aminotransferase (ALT) by 6 times, contents of urea and residual nitrogen by 2.5-3 times; level of alpha 1-protease inhibitor (alpha 1-PI) decreased by 4 times and that of alpha 2-macroglobulin (alpha 2MG) by 2.5 times. At administration of contrykal (10,000 U/kg) proteolytic activity increased only by 32.5%, content of alpha 1-PI decreased only by 10-20% and level of alpha 2-MG did not differ from that in healthy animals. Activity of AST and ALT remained high, and contents of urea and residual nitrogen were near-normal.

[Serum antiprotease activity following parenteral administration of proteolytic enzymes, histamine and acetylcholine]. / Antiproteaznaia aktivnost' syvorotki krovi pri parenteral'nom vvedenii proteoliticheskikh fermentov, gistamina i atsetilkholina.

State of tryptic and antienzymatic activities of rabbit blood serum was studied after parenteral administration of trypsin, chymotrypsin, histamine, acetylcholine and kallikrein. Treatment with chymotrypsine led to the most favorable alterations in animals as compared with trypsin and kallikrein effects. Activity of trypsin was increased and the antienzymatic activity was decreased in blood serum after administration of histamine and acetylcholine. This suggests that antiprotease drugs may be used in therapy of diseases, accompanied by increase in content of histamine and acetylcholine.

[Effect of proteolysis inhibitors on serum proteinase activity and its antitryptic activity in postischemic toxemia]. / Vliianie ingibitorov proteoliza na aktivnost' proteinaz syvorotki krovi i ee antitripticheskuiu aktivnost' pri postishemicheskom toksemii.

Parenteral administration to animals of the antikallikrein serum containing 200gamma/ml of antibodies to the pancreatic kallikrein (10 ml/kg), ingitryl (1.5 Un/kg) and of tracilol (10 000 Un/kg) exerts in post-ischemic toxemia an inhibiting effect on the protamine-splitting blood serum activity and does not affect the benzoylarginine-paranitroanilide rate of splitting. Changes of the blood serum ability to bind trypsin and manifestation of the protamine-splitting activity during the first 9 hours of observation are of an undulating nature and the changes of these characteristics are reciprocal.

[Pancreatic enzymatic activity in the blood and urine in tumors of varying localization]. / Aktivnost' pankreaticheskikh fermentov v krovi i moche pri opukholiakh razlichnoi lokalizatsii.

Under study was the activity of proteinases and their inhibitors, amylase and lipase of the blood serum and urine in 15 healthy subjects and 40 patients with tumors of different localization, and in 8 patients subjected to surgical interventions. There was found a reliable increase of total antitryptic activity in tumors of different localization and extensive operative procedures, while an increase of proteinase activity of blood serum in gastric cancer.

[Quantitative characteristics of serum antitryptic activity]. / Kil'kisna kharakterystyka antytryptychnoï iemnosti syrovatky krovi

The method suggested includes incubation of trypsin with native blood serum and subsequent quantitative determination of trypsin, that did not react with inhibitor, by means of the antitryptic precipitating serum. The method is based on the principle of radial immunodiffusion. In native serum that was not subjected to dilution the antitryptic capacity is 210.0 +/- 27.3 microng/ml in people, 238.7 +/- 33.1 microng/ml in rabbits. A distinct dynamics of changes in the antitryptic capacity of blood serum is observed with experimental hypertrypsinemia.

[Identification of proteases of staphylococci by means of an antitrypsin precipitating serum]. / Identifikatsiia proteaz stafilokokkov s pomoshch'iu antitripticheskoi pretsipitiruiushchei syvorotki

Specific antitrypsin precipitating sera obtained by intraconjunctival immunization of rabbits with crystalline trypsin was used to reveal in the supernant of staphylococcal cultures protease having antigenic character common with that of trypsin. This is confirmed by the trypsin accumulation in the process of staphylococcus cultivation, by the inhibition of the proteolytic activity of the antitrypsin precipitating sera and by the production of protein-precipitate as a result of incubation of the supernant with the antitrypsin precipitating sera.