RESUMEN
Lipids are an alternative energy source for cells and provide structural integrity in cell membrane and their metabolism is regulated with the use of different pathways, such as integrin signalling, oxidative stress, mechanical stress, and pH changes. All of those processes take place in the oral mucosa which is subject to different environmental impacts. In this study, porcine buccal pouch mucosal cells (pBPMCs) were used during long-term primary in vitro culture. The cultured cells were collected at 7, 15 and 30 days of IVC and subsequently transferred to RNA isolation. In the results of the following microarray analysis, we analyzed the genes detected, belonging to ontology groups, such as "cellular lipid metabolic process", "response to lipid" and "response to lipopolysaccharides. All of the genes involved in these ontological groups were expressed at higher levels at 7 days of IVC and substantially decreased in expression at days 15 and 30 of primary culture. We observed new genes, which may be recognized as markers in regulation of lipid metabolism in mucosal cells in vitro. The results suggested that the biochemical mechanism-involved lipids were accompanied by increased enzymatic activation and synthesis of crucial growth factors reaching high activity at day 7 of culture, which is also well documented as a stage of tissue regeneration period within oral mucosa. Therefore, this "biochemical fingerprint" may be an additional checkpoint of the integrity, resistance and easy adaptability of oral tissues, which are important conditions of success in tissue engineering and grafting for tissue reconstruction.
Asunto(s)
Expresión Génica , Metabolismo de los Lípidos , Lipopolisacáridos , Mucosa Bucal/citología , Animales , Células Cultivadas , Mejilla , Análisis de Secuencia por Matrices de Oligonucleótidos , Cultivo Primario de Células , PorcinosRESUMEN
The aim of the study was to determine the side effects of asparaginase administration during treatment protocol for childhood non-Hodgkin's lymphoma (NHL). Drug adverse reactions occurred in 20/66 of patients (30,3%) treated in 9 centres in Poland between 1993 and 1998. The most common side effects were coagulation disturbances in 12/66 of the children (18,2%), which occurred due to reduced production of important coagulation factors. Six patients (9,1%) developed impairment of liver function (9,1%). Drug toxicity caused the modifications of treatment protocol in 12/66 (18,2%) of patients, mainly in the induction phase; 3 children died due to relapse of disease.
Asunto(s)
Antineoplásicos/efectos adversos , Asparaginasa/efectos adversos , Linfoma no Hodgkin/tratamiento farmacológico , Adolescente , Anafilaxia/inducido químicamente , Antineoplásicos/administración & dosificación , Asparaginasa/administración & dosificación , Trastornos de la Coagulación Sanguínea/inducido químicamente , Enfermedad Hepática Inducida por Sustancias y Drogas , Niño , Preescolar , Diabetes Mellitus/inducido químicamente , Femenino , Humanos , Hiperlipidemias/inducido químicamente , Lactante , Masculino , Pancreatitis/inducido químicamente , Polonia , Estudios Retrospectivos , Convulsiones/inducido químicamente , Factores de TiempoRESUMEN
Sixty children with MDS treated in six centres of the Polish Paediatric Leukaemia/Lymphoma Study Group in the period 1975-1999 were included in the study. In 20 children RAEB-T, in 13 RA, in 21 RAEB and in 6 CMML were diagnosed. Our own and literature data showed that BMT is the best therapy for children with MDS. We need a new comprehensive protocol for the diagnosis and treatment of children with MDS in Poland.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Síndromes Mielodisplásicos/tratamiento farmacológico , Adolescente , Niño , Preescolar , Supervivencia sin Enfermedad , Femenino , Humanos , Lactante , Masculino , Síndromes Mielodisplásicos/fisiopatología , Polonia , Estudios Retrospectivos , Factores de Tiempo , Resultado del TratamientoRESUMEN
The aim of this study was to analyse the effect of LMB-89 protocol and surgical procedure at initial laparotomy on the outcome in children with abdominal B-cell NHL. The initial surgery intervention was: complete resection (20% pts), subtotal resection (20%), partial resection (4%), biopsy (36%). Postoperative complications occurred in 5 children. Complete recovery (CR) was achieved in 92% pts. There were 4% non responder patients. Two patients died before CR evaluation (tumour lysis syndrome; bleeding and multi organ failure after initial surgery). One patient died in CCR from sepsis probably influenced by the previous local operation. 10.8% patients relapsed. The estimate EFS for all patients with AB-NHL is 81%, 85% for stage III and 73% for stage IV. Major surgery in advanced stages is not recommended since it delays chemotherapy and fails to improve overall survival.
Asunto(s)
Neoplasias Abdominales/cirugía , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Linfoma de Células B/cirugía , Neoplasias Abdominales/tratamiento farmacológico , Neoplasias Abdominales/patología , Adolescente , Protocolos de Quimioterapia Combinada Antineoplásica/administración & dosificación , Quimioterapia Adyuvante , Niño , Preescolar , Ciclofosfamida/administración & dosificación , Citarabina/administración & dosificación , Supervivencia sin Enfermedad , Doxorrubicina/administración & dosificación , Etopósido/administración & dosificación , Femenino , Humanos , Hidrocortisona/administración & dosificación , Lactante , Laparotomía , Leucovorina/administración & dosificación , Linfoma de Células B/tratamiento farmacológico , Linfoma de Células B/patología , Masculino , Metotrexato/administración & dosificación , Prednisona/administración & dosificación , Factores de Riesgo , Factores de Tiempo , Resultado del Tratamiento , Vincristina/administración & dosificaciónRESUMEN
The aim of the study was an evaluation of circulatory system functions in patients with Wilms' tumour who underwent the multidrug chemotherapy with anthracyclines. The investigation was carried out on 43 patients after chemotherapy between 1994-1997, from 4 centres of paediatric oncology (in Gdansk, Poznan, Lublin and Bydgoszcz). All patients were divided into two groups. The children from the first group received anthracyclines without Cardioxan protection. The patients from the second group had been treated with both anthracyclines and Cardioxan. The cardiotoxicity was estimated in relation to the total dose of anthracyclines. Comparing the side effects of chemotherapy in both groups there were no essential differences in bone marrow suppression and in hepatotoxic symptoms.
Asunto(s)
Antibióticos Antineoplásicos/efectos adversos , Fármacos Cardiovasculares/administración & dosificación , Corazón/efectos de los fármacos , Neoplasias Renales/tratamiento farmacológico , Razoxano/administración & dosificación , Tumor de Wilms/tratamiento farmacológico , Adolescente , Antibióticos Antineoplásicos/farmacología , Fármacos Cardiovasculares/farmacología , Niño , Preescolar , Quimioterapia Combinada , Ecocardiografía , Femenino , Humanos , Lactante , Masculino , Razoxano/farmacología , Estudios Retrospectivos , Análisis de Supervivencia , Factores de Tiempo , Resultado del TratamientoRESUMEN
Fourty children with MDS treated in seven centres of The Polish Children's Leukemia Lymphoma Study Group in period 1975-1998y were included to the study. In 16 children RAEB-T, in 2 CMML in 10 RA and in 12 RAEB were diagnosed. Our and literature data showed that BMT is the best therapy for children with MDS. For children, who don't have a donor for BMT. Roacutan therapy seems to be the most effective.
Asunto(s)
Trasplante de Médula Ósea , Síndromes Mielodisplásicos/terapia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Niño , Preescolar , Citarabina/administración & dosificación , Femenino , Factor Estimulante de Colonias de Granulocitos y Macrófagos/uso terapéutico , Humanos , Lactante , Masculino , Mercaptopurina/administración & dosificación , Resultado del Tratamiento , Ácido Valproico/administración & dosificaciónRESUMEN
The aim of the study was to evaluate the results of treatment of 46 children with non B non-Hodgkin's Lymphoma registered in 7 centers of Polish Paediatric Leukaemia/Lymphoma Study Group from 1993 to 1998. The patients were treated according to BFM-90 protocol based on German regimen. The overall probability of event-free survival for the all children after 4 years of follow-up was 71%, for patients in stage III--65%, stage IV--70%. The achieved results were not as positive as in the BFM Study Group, what was related to: the great number of children with advanced stage of disease (54.3%), the late final diagnosis, the great number of recurrences (22.5%) and deaths caused by the toxicity of medication (6.5%) (infections, drug toxicity).
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Linfoma no Hodgkin/tratamiento farmacológico , Adolescente , Asparaginasa/administración & dosificación , Niño , Preescolar , Ciclofosfamida/administración & dosificación , Citarabina/administración & dosificación , Daunorrubicina/administración & dosificación , Femenino , Estudios de Seguimiento , Humanos , Lactante , Linfoma no Hodgkin/mortalidad , Masculino , Mercaptopurina/administración & dosificación , Metotrexato/administración & dosificación , Estadificación de Neoplasias , Polonia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamiento farmacológico , Leucemia-Linfoma Linfoblástico de Células Precursoras/mortalidad , Prednisona/administración & dosificación , Estudios Retrospectivos , Tasa de Supervivencia , Insuficiencia del Tratamiento , Vincristina/administración & dosificaciónRESUMEN
Fourty two children with myelodysplastic syndrome (MDS) treated in seven centres of The Polish Paediatric Leukaemia/Lymphoma Study Group in period 1975-1998 were included in the study. In 16 children RAEB-T, in 3 CMML, in 10 RA and in 13 RAEB were diagnosed. BMT is the best therapy for children with MDS. For children, who have not a donor for BMT, Roacutan therapy seems to be the most effective.