Investigation of the role of distances from liquid crystal monomer (LCM) factories on distribution of LCMs in surface soil samples.

Liquid crystal monomers (LCMs), which are commonly used in electronic device screens, have attracted attention as a potential class of emerging organic pollutants with persistent, bioaccumulative, and toxic (PBT) properties. This study involved the collection of 54 surface soil samples around one LC industrial park at increasing spatial distances within 1 km, 1-3 km, and 3-5 km from the center of the LC industrial park. Our observations revealed the presence of LCMs in 46 of 54 surface soil samples examined. Of the 39 target LCMs, 36 were identified, comprising 14 non-fluorinated and 22 fluorinated LCMs. Nine LCMs were detected at frequencies exceeding 20%, with 3bcHdFB exhibiting the highest detection frequency of 59% in the soil samples. The total LCM concentrations across the 46 sampling locations varied from 0.0072 to 17.24 ng/g dw, with the highest total concentrations at sampling sites within 1 km of the liquid crystal plant, suggesting that manufacturing processes may be a potential source for LCM release into the environment. Differences were observed in the LCM contamination patterns among the three sampling areas. Additionally, we observed a decrease in the median LCM concentration with increasing distance from the center of the LC industrial park. However, no statistically significant differences (p > 0.05) in LCM concentrations were observed across the three distances assessed in this study. This may be owing to the limited variety of target compounds analyzed and the limited number of soil samples. Our results emphasize that further studies on the emissions and pollution characteristics of LCMs during production are warranted.

Emerging contaminants: A One Health perspective.

Environmental pollution is escalating due to rapid global development that often prioritizes human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the continuous introduction of new substances remains a major threat to both people and the planet. In response, global initiatives are focusing on risk assessment and regulation of emerging contaminants, as demonstrated by the ongoing efforts to establish the UN's Intergovernmental Science-Policy Panel on Chemicals, Waste, and Pollution Prevention. This review identifies the sources and impacts of emerging contaminants on planetary health, emphasizing the importance of adopting a One Health approach. Strategies for monitoring and addressing these pollutants are discussed, underscoring the need for robust and socially equitable environmental policies at both regional and international levels. Urgent actions are needed to transition toward sustainable pollution management practices to safeguard our planet for future generations.

Aquatic photolysis of high-risk fluorinated liquid crystal monomers: Kinetics, toxicity evaluation, and mechanisms.

Despite the frequent detection of fluorinated liquid-crystal monomers (FLCMs) in the environment, the level of understanding of their fate, toxicity, and transformation remains insufficient. Herein, we investigated the degradation kinetics and mechanism of an FLCM (4-cyano-3-fluorophenyl 4-ethylbenzoate, CEB-F) under ultraviolet (UV) photolysis in aquatic environment. Our findings demonstrated that the UV photolysis of CEB-F followed first-order kinetics. Photodegradation products were identified using liquid chromatography with mass spectrometry, and detailed reaction pathways were proposed. It is postulated that through the attack of reactive oxygen species, hydroxylation, and CO/C-F bond cleavage, CEB-F gradually degraded into small molecular compounds, releasing fluorine ions. Acute immobilization tests with Daphnia magna (D. magna) revealed significant acute toxicity of CEB-F, with LC50 values ranging from 1.023 to 0.0536 µM over 24 to 96 h, emphasizing the potential high risk of FLCMs in aquatic ecosystems if inadvertently discharged. Interestingly, we found that the toxicity of CEB-F photolysis reaction solutions was effectively reduced. Through catalase and acetylcholinesterase activities analysis along with molecular docking simulation, we proposed differences in the underlying toxicity mechanisms of CEB-F and its photolysis products to D. magna. These findings highlight the potential harmful effects of FLCMs on aquatic ecosystems and enrich our understanding of the photolysis behavior of FLCMs.

Establishment of compound database of emerging antioxidants and high-resolution mass spectrometry screening in lake sediment from Taihu Lake Basin, China.

Antioxidants are ubiquitous in various environmental samples, leading to increasing concern regarding their potential risk to environments or humans. However, there is dearth of information regarding the environmental fate of antioxidants and unknown/unexpected antioxidants in the environment. Here, we established a compound database (CDB) containing 320 current-used antioxidants by collecting the chemicals from EPA's functional use database and published documents. Physical-chemical characteristics of these antioxidants were estimated, and 19 ones were considered as persistent and bioaccumulative (P&B) substances. This CDB was further coupled with high resolution mass spectrometry (HRMS) technique, which was employed for suspect screening of antioxidants in extracts of sediments (n = 88) collected from Taihu Lake basin. We screened 119 HRMS features that can match 135 chemical formulas in the CDB, and 20 out of them exhibited the detection frequencies ≥ 90%. The total concentrations of suspect antioxidants in sediments ranged from 6.41 to 830 ng/g dw. Statistical analysis demonstrated that concentrations of suspect antioxidants in Taihu Lake were statistically significantly lower than those in Shihu and Jiulihu Lake, but greater than those from other small lakes. Collectively, this study provided a CDB that could be helpful for further monitoring studies of antioxidant in the environments, and also provided the first evidence regarding the ubiquity of antioxidants in aquatic environment of Taihu Lake basin.

Biotransformation of Organophosphate Diesters Characterized via In Vitro Metabolism and In Vivo Screening.

Organophosphate diesters (di-OPEs), as additives in industrial applications and/or transformation products of emerging environmental pollutants, such as organophosphate triesters (tri-OPEs), have been found in the environment and biological matrices. The metabolic fate of di-OPEs in biological media is of great significance for tracing the inherent and precursor toxicity variations. This is the first study to investigate the metabolism of a suite of di-OPEs by liver microsomes and to identify any metabolite of metabolizable di-OPEs in in vitro and in vivo samples. Of the 14 di-OPEs, 5 are significantly metabolizable, and their abundant metabolites with hydroxyl, carboxyl, dealkylated, carbonyl, and/or epoxide groups are tentatively identified. More than half of the di-OPEs are detectable in human serum and/or wild fish tissues, and dibenzyl phosphate (DBzP), bis(2,3-dibromopropyl) phosphate (BDBPP), and isopropyl diphenyl phosphate (ip-DPHP) are first reported at a detectable level in humans and wildlife. Using an in vitro assay and a known biotransformation rule-based integrated screening strategy, 2 and 10 suspected metabolite peaks of DEHP are found in human serum and wild fish samples, respectively, and are then identified as phase I and phase II metabolites of DEHP. This study provides a novel insight into fate and persistence of di-OPE and confirms the presence of di-OPE metabolites in humans and wildlife.

A critical review on organophosphate esters in drinking water: Analysis, occurrence, sources, and human health risk assessment.

Organophosphate esters (OPEs) are ubiquitous in the environment. Copious studies assessed OPEs in various environmental media. However, there is limited summative information about OPEs in drinking water. This review provides comprehensive data for the analytical methods, occurrence, sources, and risk assessment of OPEs in drinking water. In general, liquid-liquid extraction and solid-phase extraction are the most common methods in the extraction of OPEs from drinking water, while gas chromatography and liquid chromatography are the most commonly used instrumental methods for detecting OPEs in drinking water. On the basis of these techniques, a variety of methods on OPEs pretreatment and determination have been developed to know the pollution situation of OPEs. Studies on the occurrence of OPEs in drinking water show that the total concentrations of OPEs vary seasonally and regionally, with tris(1-chloro-2-isopropyl) phosphate and tris(2-chloroethyl) phosphate dominant among different kinds of drinking water. Source identification studies show that there are three main sources of OPEs in drinking water: 1) source water contamination; 2) residual in drinking water treatment process; 3) leakage from device or pipeline. Besides, risk assessments indicate that individual and total OPEs pose no or negligible health risk to human, but this result may be significantly underestimated. Finally, the current knowledge gaps on the research of OPEs in drinking water are discussed and some suggestions are provided for future environmental research.

Aerobic Microbial Transformation of Fluorinated Liquid Crystal Monomer: New Pathways and Mechanism.

Fluorinated liquid crystal monomers (FLCMs) have been suggested as emerging contaminants, raising global concern due to their frequent occurrence, potential toxic effects, and endurance capacity in the environment. However, the environmental fate of the FLCMs remains unknown. To fill this knowledge gap, we investigated the aerobic microbial transformation mechanisms of an important FLCM, 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3, 5-difluoro-4'-propylbiphenyl (DTMDPB), using an enrichment culture termed as BG1. Our findings revealed that 67.5 ± 2.1% of the initially added DTMDPB was transformed in 10 days under optimal conditions. A total of 14 microbial transformation products obtained due to a series of reactions (e.g., reductive defluorination, ether bond cleavage, demethylation, oxidative hydroxylation and aromatic ring opening, sulfonation, glucuronidation, O-methylation, and thiolation) were identified. Consortium BG1 harbored essential genes that could transform DTMDPB, such as dehalogenation-related genes [e.g., glutathione S-transferase gene (GST), 2-haloacid dehalogenase gene (2-HAD), nrdB, nuoC, and nuoD]; hydroxylating-related genes hcaC, ubiH, and COQ7; aromatic ring opening-related genes ligB and catE; and methyltransferase genes ubiE and ubiG. Two DTMDPB-degrading strains were isolated, which are affiliated with the genus Sphingopyxis and Agromyces. This study provides a novel insight into the microbial transformation of FLCMs. The findings of this study have important implications for the development of bioremediation strategies aimed at addressing sites contaminated with FLCMs.

The associations between high-levels of urine benzophenone-type UV filters (BPs) and changes in serum lipid concentrations.

Little is known about the potential health impacts of benzophenone-type UV filters (BPs) exposure among the general population. In our study conducted in Wuxi, China, we investigated the associations between the concentrations of eight BP-derivatives and five target lipid molecules. We collected basic information, serum, and urine samples from 120 residents aged 9 to 80 in Wuxi. We determined BPs in urine samples and lipid levels in serum samples. Generalized linear models were used to evaluate the differences in ln-transformed serum target lipids levels (µg/L) with different urine BPs quartiles compared to the lowest quartile. Benzophenone-4 (BP-4) had the highest detection rate (95.0%) and geometric mean concentration (1.96 µg/L) among all the BP-derivatives in our study population. The exposure levels of BPs were generally higher in females than in males. Participants in the 9-17 and 18-50 age groups exhibited greater levels of exposure to BPs than those in the 51-80 age group. We observed statistically significant changes in LysoPC (18:0), LysoPE (18:0), ΣLPL, and ΣTL concentrations between the highest and lowest quartiles of BP-4. Similar changes were found in LysoPE (18:0) concentration between the highest and lowest quartiles of ΣBP-3 and ΣBPs. High urine BP concentrations were associated with variations in our target serum lipids involved in neurological and metabolic disorders, and posed a potential health risk. Future studies are warranted to further validate and elucidate our findings.

Emerging organic contaminants of liquid crystal monomers: Environmental occurrence, recycling and removal technologies, toxicities and health risks.

Liquid crystal monomers (LCMs) are a family of synthetic organic chemicals applied in the liquid crystal displays (LCDs) of various electric and electronic products (e-products). Due to their unique properties (i.e., persistence, bioaccumulative potential, and toxicity) and widespread environmental distributions, LCMs have attracted increasing attention across the world. Recent studies have focused on the source, distribution, fate, and toxicity of LCMs; however, a comprehensive review is scarce. Herein, we highlighted the persistence and bioaccumulation potential of LCMs by reviewing their physical-chemical properties. The naming rules were suggested to standardize the abbreviations regarding LCMs. The sources and occurrences of LCMs in different environmental compartments, including dust, sediment, soil, leachate, air and particulate, human serum, and biota samples, were reviewed. It is concluded that the LCMs in the environment mainly originate from the usage and disassembly of e-products with LCDs. Moreover, the review of the potential recycling and removal technologies regarding LCMs from waste LCD panels suggests that a combination of natural attenuation and physic-chemical remediation should be developed for LCMs remediations in the future. By reviewing the health risks and toxicity of LCMs, it is found that a large gap exists in their toxicity and risk to organisms. The fate and toxicity investigation of LCMs, and further investigations on the effects on the human exposure risks of LCMs to residents, especially to occupational workers, should be considered in the future.

Covalent organic frameworks bearing Ni active sites for free radical-mediated photoelectrochemical organic transformations.

Photoelectrochemical (PEC) organic transformations occurring at anodes are a promising strategy for circumventing the sluggish kinetics of the oxygen evolution reaction. Here, we report a free radical-mediated reaction instead of direct hole transfer occurring at the solid/liquid interface for PEC oxidation of benzyl alcohol (BA) to benzaldehyde (BAD) with high selectivity. A bismuth vanadate (BiVO4) photoanode coated with a 2,2'-bipyridine-based covalent organic framework bearing single Ni sites (Ni-TpBpy) was developed to drive the transformation. Experimental studies reveal that the reaction at the Ni-TpBpy/BiVO4 photoanode followed first-order reaction kinetics, boosting the formation of surface-bound ·OH radicals, which suppressed further BAD oxidation and provided a nearly 100% selectivity and a rate of 80.63 µmol hour-1 for the BA-to-BAD conversion. Because alcohol-to-aldehyde conversions are involved in the valorizations of biomass and plastics, this work is expected to open distinct avenues for producing key intermediates of great value.

Uptake, accumulation and translocation of traditional and novel organophosphate esters by rice seedlings in the presence of micro(nano)-polystyrene plastics: Effects of concentration and size of particles.

Micro(nano)plastics (MNPs) and organophosphate esters (OPEs) are becoming ubiquitous as emerging pollutants. To data, the effects of MNPs on the uptake, accumulation and translocation of OPEs by rice plant are still unclear, especially for novel OPE species. In this study, the impacts of polystyrene MNPs of different sizes and concentrations on the uptake of eight OPEs (six traditional organophosphate triesters and two novel discovered aryl organophosphate triesters) by rice seedlings were investigated in hydroponic exposure experiments. The results showed that OPEs accumulated in a concentration-dependent manner in both the roots and shoots of rice seedlings. The impacts of MNPs on uptake by rice seedlings were concentration- and size-dependent by influencing the transpiration rate or activities of antioxidant enzymes. Especially, significant effects were usually found in exposure group of medium-size and high-concentration MNPs. MNPs had more obvious effects on OPE species with lower logKow in roots, whereas, more obvious effects on OPE species with higher logKow in shoots were observed. There was a significantly positive linear relationship between logTF and logKow (p < 0.001), and a significantly negative linear relationship between logRCF and logKow (p < 0.001), indicating that OPEs with higher hydrophobicity seemed to be more liable to be absorbed from solutions to roots, but difficult to further translocate to shoots. Without novel OPEs (bis-(2-ethylhexyl)-phenyl phosphate and tris(2,4-di-tert-butylphenyl)phosphate), better fits for a linear model for logKow and logRCF (or logTF) were exhibited, indicating differences between novel and traditional OPEs. This work highlights that the presence of MNPs could altered the characteristics of uptake, translocation and accumulation of OPEs in rice seedlings, and provides an important evidence for comprehensive control strategy of new pollutants.

Established and emerging organophosphate esters (OPEs) and the expansion of an environmental contamination issue: A review and future directions.

The list of organophosphate esters (OPEs) reported in the environment continues to expand as evidenced by the increasing number of OPE studies in the literature. However, there remains a general dearth of information on more recently produced and used OPEs that are proving to be emerging environmental contaminants. The present review summarizes the available studies in a systematic framework of the current state of knowledge on the analysis, environmental fate, and behavior of emerging OPEs. This review also details future directions to better understand emerging OPEs in the environment. Firstly, we make recommendations that the current structural/practical abbreviations and naming of OPEs be revised and updated. A chemical database (CDB) containing 114 OPEs is presently established based on the suspect list from the current scientific literature. There are 12 established OPEs and a total of 83 emerging OPEs that have been reported in human and/or biota samples. Of the emerging OPEs more than 80% have nearly 100% detection frequencies in samples of certain environmental media including indoor air, wastewater treatment plants, sediment, and fish. In contrast to OPEs considered established contaminants, most emerging OPEs have been identified more recently due to the more pervasive use of high-resolution mass spectrometry (HRMS) based approaches and especially gas or liquid chromatography coupled with HRMS-based non-target analysis (NTA) of environmental sample fractions. Intentional/unintentional industrial use and non-industrial formation are sources of emerging OPEs in the environment. Predicted physical-chemical properties in silico of newer, molecularly larger and more oligomeric OPEs strongly suggest that some compounds such as bisphenol A diphenyl phosphate (BPA-DPP) are highly persistent, bioaccumulative and/or toxic. Limited information on laboratory-based toxicity data has shown that some emerging OPEs elicit harmful effects such as cytotoxicity, development toxicity, hepatotoxicity, and endocrine disruption in exposed humans and mammals. Established, and to a much lesser degree emerging OPEs, have also been shown to transform and degrade in biota and possibly alter their toxicological effects. Research on emerging OPE contaminants is presently limited and more study is warranted on sample analysis methods, source apportionment, transformation processes, environmental behavior, biomarkers of exposure and toxicity.

Organophosphate esters (OPEs) in plastic food packaging: non-target recognition, and migration behavior assessment.

Organophosphate esters (OPEs) are widely used as plasticizers in plastic food packaging; however, the migration of OPEs from plastic to food is largely unstudied. We do not even know the specific number of OPEs that exist in the plastic food packaging. Herein, an integrated target, suspect, and nontarget strategy for screening OPEs was optimized using ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS). The strategy was used to analyze 106 samples of plastic food packaging collected in Nanjing city, China, in 2020. HRMS allowed full or tentative identification of 42 OPEs, of which seven were reported for the first time. Further, oxidation products of bis(2,4-di-tert-butylphenyl) pentaerythritol diphosphite (AO626) in plastics were identified, implying that the oxidation of organophosphite antioxidants (OPAs) could be an important indirect source of OPEs in plastics. The migration of OPEs was examined with four simulated foods. Twenty-six out of 42 OPEs were detected in at least one of the four simulants, particularly isooctane, in which diverse OPEs were detected at elevated concentrations. Overall, the study supplements the list of OPEs that humans could ingest as well as provides essential information regarding the migration of OPEs from plastic food packaging to food.

Plastic packaging-associated chemicals and their hazards - An overview of reviews.

Plastic packaging contains residues from substances used during manufacturing, such as solvents, as well as non-intentionally added substances (NIAS), such as impurities, oligomers, or degradation products. By searching peer-reviewed literature, we found that at least 10,259 chemicals were related to plastic packaging materials, which include chemicals used during manufacturing and/or present in final packaging items. We then summarized and discussed their chemical structures, analytical instruments, migration characteristics, and hazard categories where possible. For plastic packaging chemicals, examination of the literature reveals gas and liquid chromatography hyphenated to a variety of accurate mass analyzers based on the use of high-resolution mass spectrometry is usually used for the identification of unknown migrants coming from plastic packaging. Chemical migration from food packaging is affected by several parameters, including the nature and complexity of the food, contact time, temperature of the system, type of packaging contact layer, and properties of the migrants. A review of the literature reveals that information on adverse effects is only available for approximately 1600 substances. Among them, it appears that additives are more toxic than monomers to wildlife and humans. Neurotoxicity accounted for the highest proportion of toxicity of all types of chemicals, while benzenoids, organic acids, and derivatives were the most toxic types of chemicals. Furthermore, studies have demonstrated that hydrocarbon derivatives, organic nitrogen compounds, and organometallic compounds have the highest proportions of dermatotoxicity, and organohalogen compounds have the highest proportions of hepatotoxicity. The main contributors to skin sensitization are organic salts. This study provides a basis for comprehensively publicizing information on chemicals in plastics, and could be helpful to better understand their potential risks to the environment and humans.

Tissue-specific uptake and distribution of liquid crystal monomers (LCMs) in mice.

Increasing evidence indicated that liquid crystal monomers (LCMs) in liquid crystal displays can be released into the environment, and ubiquitously detected in environmental matrices and even human bodies. Yet databases regarding its uptake and distribution in mammals are lacking. In this study, four LCMs (namely 3dFB, 2OdF3B, 2teFT, and 6OCB) with various physiochemical properties and structures were selected as the target compounds. The LCMs were in vivo and in vitro exposed to mice and rat liver microsomes (RLM). LCMs were found in all mouse tissues, including brain. Pharmacokinetics parameters, Cmax-tissue/Cmax-blood, ranged from 27.5 to 214, indicating the preferential deposition of LCMs to tissues rather than blood. The LCMs distributed preferentially to lipophilic tissues, and relative mass contribution of LCMs from liver and adipose was 43-98 %. The physicochemical properties (i.e., Kow, molecular weight, and functional groups) had pronounced effect on distribution and accumulation of LCMs. The 2teFT with the highest Kow and molecular weight showed the relatively higher accumulation potential and half elimination time in all the tissues. The 6OCB containing cyano-group was more accumulative than the fluorinated 3dFB with the comparable Kow. In RLM assays, 2teFT and 6OCB were resistant to metabolic degradation. While 3dFB and 2OdF3B underwent rapid degradation with 93.7 % and 72.4 % being metabolized at 360 min. Findings in this study bear significant implications for the biomonitoring and overall risk evaluation of LCMs.

Characterizing the obesogenic and fatty liver-inducing effects of Acetyl tributyl citrate (ATBC) plasticizer using both in vivo and in vitro models.

The global incidence of obesity and non-alcoholic fatty liver disease (NAFLD) is rising rapidly in recent years. Environmental factors including usage of plastics and exposure to chemicals have been proposed as important contributors to the obesity pandemic. Acetyl tributyl citrate (ATBC) is a non-phthalate plasticizer widely used in food packaging, personal care products, medical devices and children's toys etc. Due to its high leakage rate from plastics, exposure risk of ATBC keeps increasing. Although there are some studies investigating the safety of ATBC on human health, these studies mainly focused on high dosages and information regarding ATBC safety at environmental-relevant low levels is still limited. In this study, we aimed to evaluate the safety of subchronic exposure to environmentally-relevant concentrations of ATBC. C57BL/6J mice were orally exposed to ATBC for 6 or 14 weeks. Results indicated that ATBC exposure increased the body weight gain, the body fat content and the size of adipocytes, induced liver steatosis in mice. Consistent with in vivo effects, ATBC treatment increased the intracellular lipid accumulation in vitro hepatocytes. Transcriptome sequencing, qRT-PCR analysis and western blotting revealed that ATBC exposure affected the expression of genes involved in de novo lipogenesis and lipid uptake. Therefore, based on our subchronic and in vitro results, it suggested that ATBC might be a potential environmental obesogen with metabolism-disturbing and fatty liver-inducing risk, and its application in many consumer products should be carefully re-evaluated.

Liquid Crystal Monomer: A Potential PPARγ Antagonist.

Liquid crystal monomers (LCMs) are a large family of artificial ingredients that have been widely used in global liquid crystal display (LCD) industries. As a major constituent in LCDs as well as the end products of e-waste dismantling, LCMs are of growing research interest with regard to their environmental occurrences and biochemical consequences. Many studies have analyzed LCMs in multiple environmental matrices, yet limited research has investigated the toxic effects upon exposure to them. In this study, we combined in silico simulation and in vitro assay validation along with omics integration analysis to achieve a comprehensive toxicity elucidation as well as a systematic mechanism interpretation of LCMs for the first time. Briefly, the high-throughput virtual screen and reporter gene assay revealed that peroxisome proliferator-activated receptor gamma (PPARγ) was significantly antagonized by certain LCMs. Besides, LCMs induced global metabolome and transcriptome dysregulation in HK2 cells. Notably, fatty acid ß-oxidation was conspicuously dysregulated, which might be mediated through multiple pathways (IL-17, TNF, and NF-kB), whereas the activation of AMPK and ligand-dependent PPARγ antagonism may play particularly important parts. This study illustrated LCMs as a potential PPARγ antagonist and explored their toxicological mode of action on the trans-omics level, which provided an insightful overview in future chemical risk assessment.

Target and suspect analysis of liquid crystal monomers in soil from different urban functional zones.

Recent studies have reported the occurrence of liquid crystal monomers (LCMs) in sediment, indoor dust, hand wipes, and human serum samples; however, information regarding their contamination status in soil is currently unavailable. The concentrations of 39 target LCMs were determined in n = 96 surface soil samples collected from five different urban functional zones including agricultural, scenic, industrial, commercial, and residential zones. We observed that 76 of 96 surface soil samples contained at least 19, 13, 16, 19, and 14 of the 39 target LCMs that were detectable in samples from agricultural, scenic, industrial, commercial, and residential zones, respectively. The LCMs in the samples from the agricultural zone exhibited the highest mean concentrations of 12.9 ng/g dry weight (dw), followed by those from commercial (5.23 ng/g dw), residential (3.30 ng/g dw), industrial (2.48 ng/g dw), and scenic zones (0.774 ng/g dw). Furthermore, strong and statistically significant (p < 0.05) correlations were observed for several pairs of LCMs (3cH2B vs. 5bcHdFB in the agricultural zone; 5bcHdFB vs. 2bcHdFB, 5bcHdFB vs. 3cH2B in the commercial zone; 5bcHdFB vs. 2bcHdFB in the industrial zone), indicating that they might have similar commercial applications and sources. Based on a newly established database containing 1173 LCMs, suspect screening was applied to discover other LCMs in these 96 soil samples using gas chromatograph coupled with quadrupole-time-of-flight mass spectrometry (GC-QTOF/MS). We tentatively identified 51 LCM formulas with 69 chemical structures. Collectively, this study provides the first evidence for the occurrence of LCMs in soil samples, and suggests that LCMs could be widely distributed across all five urban functional zones.

Tissue-specific accumulation, bioaccumulation, and depuration of liquid crystal monomers (LCMs) in adult zebrafish (Danio rerio).

This study investigated the properties of bioaccumulation, tissue-specific accumulation, and depuration of liquid crystal monomers (LCMs) in adult zebrafish (Danio rerio) exposed to a mixture of 39 LCMs for 19 days followed by depuration for 12 days. Tissue-specific accumulation of LCMs was examined at the time point of day 19, and we observed that the distribution of LCMs varied among different tissues with the following order of Σ39LCM concentrations, the intestine > brain > gill > liver > muscle. We also observed that the bioaccumulation potential of LCMs varied among LCM groups with different functional groups, and LCMs with the cyan group were prone to accumulate in zebrafish. Among the 39 target LCMs, nine exhibited bioconcentration factors (BCFs) >1000, indicating their great bioaccumulation potential in aquatic environments. The experimental BCFs of 22 LCMs in the present study were lower than the theoretical values predicted by the Estimation Programs Interface (EPI) Suite software developed by U.S. Environmental Protection Agency (USEPA), suggesting that their bioaccumulation potential might be overestimated by theoretical estimation techniques. Another interesting finding was the significant positive correlation relationship in both sexes of zebrafish (p < 0.01, r2 = 0.66 for male; p < 0.01, r2 = 0.41 for female) between logBCFww and logKow values of LCMs. Overall, this study provides fundamental information regarding the bioaccumulation potentials of LCMs, which could be helpful for further investigating the health risks of LCMs in aquatic environments.