Ensemble learning based assessment of the role of transcription factors in gene expression.

Cancer cells are formed when the associated, active genes fail to function the way they are meant to function. Multiple genes collectively control cell growth by activating a proper set of genes. Regulation of gene expression is controlled through the combined effort of multiple regulatory elements. Transcription of each gene is affected differently according to the combinatorial patterns of regulatory elements bound in the nearby regions. Identifying and analysing such patterns will give a better insight into the cell function. The main focus of this study is on developing a computational model to predict the functional role of transcriptional factors residing between divergent gene pairs. Acute Myeloid Leukaemia (AML) gene expression data from GEO and the two TFs EP300 and CTCF binding data calibrated in k562 cell line from ENCODE consortium are taken as a case study.

Monitoring chlorine residual and trihalomethanes in the chlorinated seawater effluent of a nuclear power plant.

Periodic sampling of the discharged seawater effluent from Madras Atomic Power Station (Kalpakkam, Tamil Nadu, India) was carried out during 2013-2017 to assess the residual chlorine and trihalomethanes content in the outfall discharge water. The variations in dissolved oxygen, temperature, and pH were correlated with the residual chlorine and trihalomethanes content in the discharged effluent. The difference in temperature (ΔT) between influent and effluent seawater samples ranged from 1.95 to 11.0 °C (6.47 ± 1.87). More than 95% of the ΔT values were within the guideline value of 7 °C. The discharge water was associated with a marginal reduction in dissolved oxygen and a marginal increase in conductivity values. The total residual chlorine content in the discharged seawater at outfall ranged from 0.06 to 0.42 (0.16 ± 0.08) mg/L, which was within the stipulated values of 0.5 mg/L. Trihalomethanes values ranged from 0.04 to 65.03 (13.06 ± 14.38) µg/L. In addition to bromoform as the major constituent, occurrence of significant amount chloroform of was occasionally observed in the discharge water.